Abstract:
BACKGROUND:Acute bacterial meningitis (ABM) is a serious disease that needs rapid diagnosis for an accurate treatment. The most important etiological agents are: Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae type b. Overall pathogen detection rate in patients with ABM in Chile is 83%. AIM:To evaluate a Polymerase Chain Reaction (PCR) protocol for simultaneous detection of several pathogens in patients with ABM. MATERIAL AND METHODS:We designed and evaluated a multiplex PCR protocol for simultaneous specific genes identifications of S pneumoniae ( inverted exclamation markytA and ply genes), N meningitidis (ctrA, crgA) and H influenzae (bexA) in cerebrospinal fluid (CSF) samples from pediatric patients with suspected diagnosis of ABM. Sensitivity, specificity and minimum detection levels of DNA were determined. Amplifications ofrDNA 16S gene was done to confirm extraction of bacterial DNA. RESULTS:Ninety nine CSF samples were studied, 90 from children with fever and negative CSF culture, and 9 from ABM and positive culture patients. The PCR protocol had a sensitivity of 89%, specificity of 100%, positive predictive value 100% and negative predictive value 99%. CONCLUSIONS:We observed a high concordance (89%) between bacteriological cultures and the PCR protocol results. This diagnostic tool could increase identification of agents in specific settings such as patients previously treated with antibiotics.
journal_name
Rev Med Chiljournal_title
Revista medica de Chileauthors
Reyes S M,Torres T JP,Prado J V,Vidal A Rdoi
/S0034-98872008000300009subject
Has Abstractpub_date
2008-03-01 00:00:00pages
338-46issue
3eissn
0034-9887issn
0717-6163pii
S0034-98872008000300009journal_volume
136pub_type
杂志文章abstract::A 68 years old male presented with right hypochondrium abdominal pain and jaundice with no other clinical finding. CAT Scan and Ultrasonography showed right lobe bile duct dilatation. Magnetic Resonance Cholangiopancreatography gave an outstanding vision of a restricted right lobe bile duct dilatation associated to ch...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
abstract:BACKGROUND:Patients on chronic hemodialysis have a lower immune response to vaccination against hepatitis B virus (HBV) than the general population. AIM:To identify factors that may interfere with immunization against hepatitis B virus (HBV) in Brazilian hemodialysis patients and analyze the evolution of the level of ...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/S0034-98872012000700008
更新日期:2012-07-01 00:00:00
abstract:BACKGROUND:Petcoke is a remnant of the oil refining process that contains Ni and other heavy metals. Undesired human exposure to these compounds may result from petcoke combustion in plants located in the vicinity of the cities. AIM:To compare levels of urinary Ni in schoolchildren residing in cities exposed and not e...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:/S0034-98872008000800013
更新日期:2008-08-01 00:00:00
abstract:BACKGROUND:Scientific Ethics is the theory and praxis of decisions. Philosophical Ethics is presented as the theory and praxis of the good. As the good differs among cultures, Philosophical Ethics is dependent on the endo-cultural good conception. The decision (included that one of adhesion or not to a world vision) de...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:2001-05-01 00:00:00
abstract:BACKGROUND:The implementation of the marketing strategies in public hospitals provides management advantages and improves the relationship between customers and staff. AIM:To analyze the application of marketing strategies in a public hospital, from the perspective of the staff. MATERIAL AND METHODS:A structured surv...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/s0034-98872006000300014
更新日期:2006-03-01 00:00:00
abstract::In this issue of the journal Lanas et al report an estimation of population attributable risk (PAR) for myocardial infarction (MI) derived from different risk factors in Chile. Cigarette smoking, dyslipidemia and hypertension accounted for 71% of total PAR. Mortality from MI has decreased in Chile, but epidemiologic s...
journal_title:Revista medica de Chile
pub_type: 评论,社论
doi:/S0034-98872008000500001
更新日期:2008-05-01 00:00:00
abstract::Increased cholesterol levels above 200 mg/dl, LDL levels above 130 mg/dl and total cholesterol/HDL ratio above 4.5 in males and above 5.0 in females are recognized as indicators of increased risk of atherosclerosis. Risk associated to increased triglyceride levels (above 200 mg/dl) must be judged in relation to associ...
journal_title:Revista medica de Chile
pub_type: 杂志文章,评审
doi:
更新日期:1991-09-01 00:00:00
abstract:BACKGROUND:Neonatal alloimmune thrombocytopenia (NAIT) is a result of fetomaternal incompatibility. Platelet destruction is caused by a maternal antibody directed against a fetal platelet antigen inherited from the father and lacking on the mother's platelets. The incidence and features of transplacental alloimmunizati...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1997-12-01 00:00:00
abstract::We describe findings in 188 patients with Berger's disease, the most frequent primary glomerulopathy in our renal biopsy material (25%). Diagnosis was made by finding IgA and dense mesangial deposits with immunofluorescence and electronmicroscopy, respectively. Patient's age ranged from 3 to 64 years (mean 27), 72 wer...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1990-02-01 00:00:00
abstract::Considering that a high proportion of the Chilean general population smokes, the Chilean Society of Respiratory Diseases in collaboration with the Chilean Societies of Cardiology and, Endocrinology and Diabetes, formed an interdisciplinary group, who issued a set of recommendations for the treatment of the smoker, met...
journal_title:Revista medica de Chile
pub_type: 杂志文章,实务指引
doi:10.4067/s0034-98872017001101471
更新日期:2017-11-01 00:00:00
abstract::We report a 52 years old male admitted for fever lasting one month, dry cough, headache and malaise. Initial laboratory work up showed an AST of 172 U/l, and ALT of 252 U/l, a GGT of 353 U/l and alkaline phosphatases of 952 U/l. An abdominal CAT scan disclosed a mild hepatosplenomegaly. A liver biopsy showed a granulo...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:2002-11-01 00:00:00
abstract:BACKGROUND:Dopamine agonists (DA) effectively reduce tumor size of macroprolactinomas, with the consequent improvement of eventual visual impairment. AIM:To study the visual outcomes in patients with macroprolactinoma treated with DA. MATERIAL AND METHODS:Retrospective cohort study which included patients with macrop...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/S0034-98872013000600001
更新日期:2013-06-01 00:00:00
abstract::Both epidemiological and clinical evidence suggest a relationship between the prenatal environment and the risk of developing diseases during adulthood. The first observations about this relationship showed that prenatal growth retardation or stress conditions during fetal life were associated to cardiovascular, metab...
journal_title:Revista medica de Chile
pub_type: 杂志文章,评审
doi:10.4067/s0034-98872006000100015
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Acute appendicitis is the most common non obstetric surgical emergency during pregnancy. AIM:To asses our experience in the diagnosis and management of acute appendicitis occurring during pregnancy. PATIENTS AND METHODS:Data from all pregnant patients who were subjected to an appendectomy for a suspected a...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/s0034-98872006000200002
更新日期:2006-02-01 00:00:00
abstract::This article deals with the history and evolution of student's scientific journals in Latin-America, their beginnings, how many still exist and which is their future projection. Relevant events show the growth of student's scientific journals in Latin-America and how are they working together to improve their quality....
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:/S0034-98872010001200016
更新日期:2010-11-01 00:00:00
abstract::Microbiology starts in Chile at the end of the XIXth century as part of the effort to develop basic sciences at the University of Chile Medical School. Publications by Vicente Izquierdo on gonorrheae and Alejandro del Río on the organisms of dysenteriae were pioneer works in this area. The field was progressively deve...
journal_title:Revista medica de Chile
pub_type: 历史文章,杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::We compared 2 techniques for determination of plasma levels of phenytoin, liquid-gas chromatography (LGC) and homogeneous enzymatic immunoassay (EI) in samples from 72 patients receiving monotherapy with this drug. Determinations were also made in samples of normal plasma with known concentrations of phenytoin. Good c...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1989-08-01 00:00:00
abstract::Environmental factors may influence the development of Inflammatory Bowel Disease and modify its natural history. The objective of this review is to evaluate current evidence about environmental factors associated with the disease. A better knowledge about the pathogenesis of the disease can lead to better treatment s...
journal_title:Revista medica de Chile
pub_type: 杂志文章,评审
doi:10.4067/s0034-98872019000200212
更新日期:2019-02-01 00:00:00
abstract::A 62 years old male with a slowly progressive focal myopathy is presented. He had noticed weakness in the lower limbs for three years and weakness in the upper limbs for one year. He had bilateral atrophy of quadriceps and biceps muscles, absence of knee jerks and hypertrophy of the calves. Needle EMG showed myopathic...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::Polycystic ovarian syndrome (PCOS) is an endocrine and metabolic dysfunction, highly prevalent in women in their reproductive years. Hyperandrogenism, oligo-ovulation, polycystic ovarian morphology are the main features of this syndrome. PCOS is a genetic disorder with a multifactorial etiology and has a strong link w...
journal_title:Revista medica de Chile
pub_type: 杂志文章,评审
doi:10.4067/s0034-98872017000700907
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abstract:BACKGROUND:Diagnostic scores have been developed as simple, user-friendly, cost-effective instruments to improve the early diagnosis of acute appendicitis. AIM:To assess the yield of a diagnostic score for acute appendicitis, measured by trained health-related non-physician professional. PATIENTS AND METHODS:A prospe...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/s0034-98872006000100005
更新日期:2006-01-01 00:00:00
abstract::We evaluated pharmacokinetic parameters and bioavailability of 4 sustained release theophylline preparations. A crossover design was used in 12 healthy males aged 22 to 27 years old. Each individual received 250 mg iv followed by 250 mg orally of each preparation, and then 400 mg rapid acting aminophylline. A 7 day pe...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1990-11-01 00:00:00
abstract:BACKGROUND:Hürthle cells can be found in non malignant thyroidal diseases such as Basedow Graves and Hashimoto diseases. When Hürthle cells comprise more the 75% of cellularity, they become a neoplasm. There are malignant variants of these neoplasms, constituted by follicular and papillary Hürthle cell carcinomas. AIM...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract::Pancreatic choristoma is the occurrence of normal pancreatic tissue in an abnormal location without any anatomic continuity with the main body of the gland. Although heterotopia is uncommon in the gallbladder and biliary tract, anecdotic cases of gastric mucosa, liver, adrenal gland and pancreas among other tissues ha...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:/S0034-98872007001000013
更新日期:2007-10-01 00:00:00
abstract:BACKGROUND:With the aim of contributing to he Healthy Goal 2010 of reducing significantly the prevalence of childhood obesity we developed and implemented during 2003 and 2004, a school-based obesity prevention intervention which included nutrition education and the promotion of physical activity. AIM:To report the re...
journal_title:Revista medica de Chile
pub_type: 杂志文章,多中心研究
doi:/S0034-98872008000100003
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Systemic fungal infections and specifically invasive aspergillosis (IA) are associated with a high morbi-mortality rate in patients with hematologic malignancies. Itraconazole kinetic studies show that plasma levels are not satisfactory, even though there is a reduction of the severity in clinical cases. AI...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:/S0034-98872011000900003
更新日期:2011-09-01 00:00:00
abstract::We report a 44 years old male, presenting with an eight months history of right lower quadrant pain, diarrhea and weight loss. Colonoscopy showed a proliferative and ulcerated lesion in the cecum, with necrotic areas. Barium enema showed an extensive irregular stenosis with rigidity of cecum and ascending colon. The e...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract:BACKGROUND:Glutathione peroxidase (GP) can be used as a marker of oxidative stress in infectious diseases. AIM:To evaluate the association between the levels of glutathione peroxidase (GP) and the manifestations and complications of dengue. PATIENTS AND METHODS:Between April 2003 and December 2004, 161 patients with ...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:10.4067/s0034-98872007000600008
更新日期:2007-06-01 00:00:00
abstract::There is a close link between hypertension and atherosderosis. Hypertension causes atherosclerotic damage of several organs, called target organs and the risk factors for hypertension and atherosderosis are very similar. The risk of mortality associated to hypertension increases with blood pressure values below the cu...
journal_title:Revista medica de Chile
pub_type: 杂志文章,评审
doi:/S0034-98872010000300015
更新日期:2010-03-01 00:00:00
abstract:BACKGROUND:The few studies in Chile assessing the nutritional status of indigenous children show a high prevalence of stunting, excess weight and feeding problems. AIM:To compare anthropometric indices in children from indigenous and non indigenous ancestry. SUBJECTS AND METHODS:School children aged 6 to 8 years old,...
journal_title:Revista medica de Chile
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00