当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Brome mosaic virus coat protein inhibits viral RNA synthesis in vitro.

Brome mosaic virus coat protein inhibits viral RNA synthesis in vitro.

Abstract：

:In vitro, the coat protein of brome mosaic virus (BMV) inhibited RNA synthesis by replicase extracted from BMV-infected barley leaves. The inhibition was due to the interaction of coat protein with BMV RNA. Under the same conditions, no inhibition by TMV coat protein or bovine serum albumin was detected. For the complete inhibition of RNA synthesis in vitro, a coat protein:RNA ratio of 620:1 was required, and their preincubation before addition to the reaction mixture was essential. If the coat protein was added to the reaction mixture during incubation, synthesis continued for a few minutes at the level of the control (omission of coat protein), then decreased gradually, and stopped 6 min after the addition of coat protein. These results suggest that the inhibitory effect of coat protein on RNA synthesis in vitro is due to the interference with the binding site of replicase by partial reassembly of nucleoprotein and that this phenomenon may be a cause of cross protection.

journal_name

Virology

journal_title

Virology

authors

Horikoshi M,Nakayama M,Yamaoka N,Furusawa I,Shishiyama J

doi

10.1016/0042-6822(87)90232-7

subject

Has Abstract

pub_date

1987-05-01 00:00:00

pages

15-9

issue

1

eissn

0042-6822

issn

1096-0341

pii

0042-6822(87)90232-7

journal_volume

158

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Identification of RNA ligands that bind hepatitis C virus polymerase selectively and inhibit its RNA synthesis from the natural viral RNA templates.

    abstract：:To identify the potential RNA inhibitors of HCV polymerase, we have isolated high-affinity RNA ligands specific to hepatitis C virus (HCV) NS5B protein from a combinatorial RNA library using the Systematic Evolution of Ligands by EXponential enrichment (SELEX) procedure. Thirty-seven selected ligands were classified i...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(02)00095-8

    authors： Vo NV,Oh JW,Lai MM

    更新日期：2003-03-15 00:00:00

  • The genome of the archaeal virus SIRV1 has features in common with genomes of eukaryal viruses.

    abstract：:The virus SIRV1 of the extremely thermophilic archaeon Sulfolobus has a double-stranded DNA genome similar in architecture to the genomes of eukaryal viruses of the families Poxviridae, Pycodnaviridae, and Asfarviridae: the two strands of the 32,301 bp long linear genome are covalently connected forming a continuous p...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0776

    authors： Blum H,Zillig W,Mallok S,Domdey H,Prangishvili D

    更新日期：2001-03-01 00:00:00

  • Canine parvovirus capsid assembly and differences in mammalian and insect cells.

    abstract：:We examined the assembly processes of the capsid proteins of canine parvovirus (CPV) in mammalian and insect cells. In CPV-infected cells empty capsids assembled within 15 min, and then continued to form over the following 1 h, while full (DNA-containing) capsids were detected only after 60 min, and those accumulated ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0734

    authors： Yuan W,Parrish CR

    更新日期：2001-01-20 00:00:00

  • Antigen specificity of CD4 T cell response in the central nervous system of mice infected with mouse hepatitis virus.

    abstract：:Previously, we showed that the transmembrane (M) and surface (S) glycoproteins were recognized by splenic CD4 T lymphocytes harvested from mice infected intraperitoneally with mouse hepatitis virus, strain JHM (MHV-JHM), whereas only the S protein was recognized by splenocytes derived from mice with MHV-induced chroni...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8819

    authors： Xue S,Perlman S

    更新日期：1997-11-10 00:00:00

  • Map location of lactate dehydrogenase-elevating virus (LDV) capsid protein (Vp1) gene.

    abstract：:Lactate dehydrogenase-elevating virus (LDV) is currently classified within the Togaviridae family. In an effort to obtain further information on the characteristics of this virus, we have begun to sequence the viral RNA genome and to map the virion structural protein genes. A sequence of 1064 nucleotides, which repres...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90546-4

    authors： Godeny EK,Speicher DW,Brinton MA

    更新日期：1990-08-01 00:00:00

  • Immunogenicity of a plant-derived edible rotavirus subunit vaccine transformed over fifty generations.

    abstract：:Major efforts have been put forth for the development of effective rotavirus vaccines including transgenic plant vaccines. Previous studies have reported that rotavirus VP7 maintains its neutralizing immunity when it is transformed into the potato genome. The present study was aimed at investigating the hereditary sta...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.07.045

    authors： Li JT,Fei L,Mou ZR,Wei J,Tang Y,He HY,Wang L,Wu YZ

    更新日期：2006-12-05 00:00:00

  • Amino acid sequence analysis of bovine rotavirus B223 reveals a unique outer capsid protein VP4 and confirms a third bovine VP4 type.

    abstract：:The nucleotide and deduced amino acid sequence of the gene 4 of bovine rotavirus strain B223 is described. The open reading frame is predicted to encode a VP4 of 772 amino acids, shorter than described for any other rotavirus strain sequenced to date. B223 VP4 shows 70 to 73% similarity to other rotavirus VP4 proteins...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90191-q

    authors： Hardy ME,Gorziglia M,Woode GN

    更新日期：1992-11-01 00:00:00

  • Histones as a target for influenza virus matrix protein M1.

    abstract：:Matrix protein M1 purified from influenza A and B viruses has been analyzed for its ability to specifically interact with cellular proteins by immune coprecipitation and by an in vitro binding assay on nitrocellulose on PVDF membranes. When M1 was mixed with lysates of uninfected cells there was selective binding of h...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8700

    authors： Zhirnov OP,Klenk HD

    更新日期：1997-09-01 00:00:00

  • Molecular and serological characterization of SARS-CoV-2 infection among COVID-19 patients.

    abstract：BACKGROUND:SARS-CoV-2 is a novel coronavirus and the cause of COVID-19. More than 80% of COVID-19 patients exhibit mild or moderate symptoms. In this study, we investigated the dynamics of viral load and antibodies against SARS-CoV-2 in a longitudinal cohort of COVID-19 patients with severe and mild/moderate diseases. ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2020.09.008

    authors： Li L,Liang Y,Hu F,Yan H,Li Y,Xie Z,Huang L,Zhao J,Wan Z,Wang H,Shui J,Cai W,Tang S

    更新日期：2020-12-01 00:00:00

  • Neutralizing monoclonal antibodies to human immunodeficiency virus type 1 do not inhibit viral transcytosis through mucosal epithelial cells.

    abstract：:HIV-1 transcytosis has been proposed as a potential mechanism allowing the virus to cross the epithelium during mucosal transmission. Epitopes of the HIV-1 envelope involved in this process have not been identified yet. Here, we assessed a large panel of HIV neutralizing antibodies recognizing well-characterized epito...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.09.006

    authors： Chomont N,Hocini H,Gody JC,Bouhlal H,Becquart P,Krief-Bouillet C,Kazatchkine M,Bélec L

    更新日期：2008-01-20 00:00:00

  • Synthesis of a full-length infectious cDNA clone of cucurbit aphid-borne yellows virus and its use in gene exchange experiments with structural proteins from other luteoviruses.

    abstract：:A full-length cDNA of cucurbit aphid-borne yellows virus (CABYV) has been constructed and expressed either as an in vitro transcript, under control of a bacteriophage T7 RNA polymerase promoter, or in vivo, under control of the cauliflower mosaic virus 35S promoter in an agroinfection vector. The biological activity o...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.9945

    authors： Prüfer D,Wipf-Scheibel C,Richards K,Guilley H,Lecoq H,Jonard G

    更新日期：1995-12-01 00:00:00

  • Cloning of the genome of a densovirus and rescue of infectious virions from recombinant plasmid in the insect host Spodoptera littoralis.

    abstract：:We have cloned an infectious genome of the Junonia coenia densonucleosis virus (JcDNV) into the bacterial plasmid pBR322. The viral genome could be rescued from the recombinant plasmid pBRJ by transfection of pBRJ DNA to sensitive Spodoptera littoralis larvae. pBRJ DNA produced a typical viral infection and a comparab...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90308-e

    authors： Jourdan M,Jousset FX,Gervais M,Skory S,Bergoin M,Dumas B

    更新日期：1990-11-01 00:00:00

  • Grapevine viroid 1B, a new member of the apple scar skin viroid group contains the left terminal region of tomato planta macho viroid.

    abstract：:GV1B is one of five viroids that have recently been purified from grapevines. GV1B has now been sequenced and its 363 nucleotide residues can potentially form the typical rod-like structure of viroids with 67% of nucleotides base-paired. GV1B has highest sequence similarity with grapevine yellow speckle viroid (GYSV; ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90450-9

    authors： Koltunow AM,Rezaian MA

    更新日期：1989-06-01 00:00:00

  • Cucumber mosaic virus-induced RNA replicase: solubilization and partial purification of the particulate enzyme.

    abstract：:Cucumber seedlings infected with cucumber mosaic virus (CMV) contain a virus-induced RNA-dependent RNA polymerase (RNA replicase) in both soluble and particulate fractions of plant extracts; no such activity is found in uninfected plants. The extensive purification and characterization of the soluble RNA replicase has...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(81)90131-8

    authors： Gill DS,Kumarasamy R,Symons RH

    更新日期：1981-08-01 00:00:00

  • Insights into the control of geminiviral promoters.

    abstract：:Geminiviruses constitute one of the largest groups of plant viruses, having characteristic twinned geminate particles encapsidating small circular single-stranded DNA molecules. Geminiviral promoters are generally located within the intergenic region, although promoters have also been detected within the genes. Simila...

    journal_title：Virology

    pub_type： 杂志文章,评审

    doi：10.1016/j.virol.2016.04.033

    authors： Borah BK,Zarreen F,Baruah G,Dasgupta I

    更新日期：2016-08-01 00:00:00

  • Antigenic and sequence variation in the C-terminal unique domain of the Epstein-Barr virus nuclear antigen EBNA-1.

    abstract：:The Epstein-Barr virus (EBV) nuclear antigen EBNA-1 is essential for viral genome maintenance in vitro and may be the only EBV protein expressed by the majority of latently infected cells in vivo. EBNA-1 may therefore be critical to the evasion of host immunity which allows persistent infection. EBNA-1 includes a poly...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1183

    authors： Wrightham MN,Stewart JP,Janjua NJ,Pepper SD,Sample C,Rooney CM,Arrand JR

    更新日期：1995-04-20 00:00:00

  • Isolation of a Friend recombinant polytropic virus with a T-cell-restricted leukemogenicity.

    abstract：:Hematopoietic malignant cells of various types were isolated from ICFW mice inoculated as newborn or adult with F-MuLV and grafted into recipient mice. After repeated in vivo cell transplants, several recombinant polytropic viruses (also termed MCF or dualtropic viruses) were isolated from tumors by limiting dilution....

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90360-4

    authors： Della-Valle V,Wendling F,Sitbon M,Varlet P,Mathieu-Mahul D,Larsen CJ

    更新日期：1990-10-01 00:00:00

  • HIV-1 Vpu's lipid raft association is dispensable for counteraction of the particle release restriction imposed by CD317/Tetherin.

    abstract：:HIV-1 Vpu antagonizes the block to particle release mediated by CD317 (BST-2/HM1.24/Tetherin) via incompletely understood mechanisms. Vpu and CD317 partially reside in cholesterol-rich lipid rafts where HIV-1 budding preferentially occurs. Here we find that lipid raft association of ectopically expressed or endogenous...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.12.008

    authors： Fritz JV,Tibroni N,Keppler OT,Fackler OT

    更新日期：2012-03-01 00:00:00

  • Biochemical studies of the maturation of herpesvirus nucleocapsid species.

    abstract：:Three distinct species of nucleocapsids of equine herpesvirus type-1, designated as either light (L), intermediate (I), or heavy (H) on the basis of their densities in Renografin-76 density gradients (Perdue et al. 1975), were characterized with respect to their amino acid content, DNA content, and role in the maturat...

    journal_title：Virology

    pub_type： 杂志文章

    doi：

    authors： Perdue ML,Cohen JC,Randall CC,O'Callaghan DJ

    更新日期：1976-10-01 00:00:00

  • Vaccinia virus-mediated inhibition of host protein synthesis involves neither degradation nor underphosphorylation of components of the cap-binding eukaryotic translation initiation factor complex eIF-4F.

    abstract：:Recent reports indicated that vaccinia virus late mRNAs contain a unique 5' poly(A) leader sequence and that the in vitro translation of these mRNAs may be relatively cap-independent. These observations led us to examine the possibility that the mechanism of inhibition of host protein synthesis by vaccinia virus, like...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90556-5

    authors： Schnierle BS,Moss B

    更新日期：1992-06-01 00:00:00

  • A system for the analysis of BKV non-coding control regions: application to clinical isolates from an HIV/AIDS patient.

    abstract：:The human polyomavirus BK virus (BKV) is an important opportunistic pathogen whose disease prevalence continues to increase with the growing immunocompromised population. To date, the major determinant of replication in cell culture has not been formally proven. BKV exists as archetype virus and rearranged variants, w...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.08.032

    authors： Broekema NM,Abend JR,Bennett SM,Butel JS,Vanchiere JA,Imperiale MJ

    更新日期：2010-11-25 00:00:00

  • Deep sequencing of H7N8 avian influenza viruses from surveillance zone supports H7N8 high pathogenicity avian influenza was limited to a single outbreak farm in Indiana during 2016.

    abstract：:In mid-January 2016, an outbreak of H7N8 high-pathogenicity avian influenza virus (HPAIV) in commercial turkeys occurred in Indiana. Surveillance within the 10km control zone identified H7N8 low-pathogenicity avian influenza virus (LPAIV) in nine surrounding turkey flocks but no other HPAIV-affected premises. We seque...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2017.04.025

    authors： Lee DH,Torchetti MK,Killian ML,Swayne DE

    更新日期：2017-07-01 00:00:00

  • The prolonged culture of human immunodeficiency virus type 1 in primary lymphocytes increases its sensitivity to neutralization by soluble CD4.

    abstract：:Primary strains of human immunodeficiency virus type 1 (HIV-1) are known to adapt to replication in cell lines in vitro by becoming sensitive to soluble CD4 (sCD4) and neutralizing antibodies (NAb). T-cell lines favor isolation of variants that use CXCR4 as a co-receptor, while primary isolates predominantly use CCR5....

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2003.12.012

    authors： Pugach P,Kuhmann SE,Taylor J,Marozsan AJ,Snyder A,Ketas T,Wolinsky SM,Korber BT,Moore JP

    更新日期：2004-03-30 00:00:00

  • Herpes simplex virus 1 infection is required to produce ICP27 immunoreactive triplet forms when ribosomal aminoacyl-tRNA translocation is blocked by cycloheximide.

    abstract：:Infected cell protein (ICP) 27 is an essential herpes simplex virus type 1 (HSV-1) phosphoprotein required for optimal viral DNA and early or late gene synthesis. Three slow-migrating immunoreactive species were detected using multiple anti-ICP27 antibodies following HSV-1 infection of HEp-2 and Vero cells in the pres...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.04.011

    authors： Sanfilippo CM,Lombardozzi RC,Chirimuuta FN,Blaho JA

    更新日期：2004-07-01 00:00:00

  • A restricted component of the Epstein-Barr virus early antigen complex is structurally related to ribonucleotide reductase.

    abstract：:The 85-kDa polypeptide previously shown to be associated with the restricted (R) component of the EBV-induced early antigen (EA) complex was subjected to amino acid sequencing analysis. This was accomplished by cyanogen bromide cleavage and by separation of individual peptides by high-pressure liquid chromatography em...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90331-x

    authors： Goldschmidts W,Luka J,Pearson GR

    更新日期：1987-03-01 00:00:00

  • Coronavirus E protein forms ion channels with functionally and structurally-involved membrane lipids.

    abstract：:Coronavirus (CoV) envelope (E) protein ion channel activity was determined in channels formed in planar lipid bilayers by peptides representing either the transmembrane domain of severe acute respiratory syndrome CoV (SARS-CoV) E protein, or the full-length E protein. Both of them formed a voltage independent ion cond...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2012.07.005

    authors： Verdiá-Báguena C,Nieto-Torres JL,Alcaraz A,DeDiego ML,Torres J,Aguilella VM,Enjuanes L

    更新日期：2012-10-25 00:00:00

  • Ceramide formation mediated by acid sphingomyelinase facilitates endosomal escape of caliciviruses.

    abstract：:Our recent results demonstrated that bile acids facilitate virus escape from the endosomes into the cytoplasm for successful replication of porcine enteric calicivirus (PEC). We report a novel finding that bile acids can be substituted by cold treatment for endosomal escape and virus replication. This endosomal escape...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2015.04.022

    authors： Shivanna V,Kim Y,Chang KO

    更新日期：2015-09-01 00:00:00

  • Tyrosine sulfation of varicella-zoster virus envelope glycoprotein gpl.

    abstract：:Sulfation is a common post-translational modification of secreted and membrane proteins, with the sulfate attached to tyrosine residues or to glycan side-chains. I have shown that varicella-zoster virus (VZV) envelope glycoproteins gpI, gpII, and gpIII can be labeled with [35S]sulfate. The predominant VZV glycoprotein...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1576

    authors： Edson CM

    更新日期：1993-11-01 00:00:00

  • Interaction of HCMV UL84 with C/EBPalpha transcription factor binding sites within oriLyt is essential for lytic DNA replication.

    abstract：:Human cytomegalovirus (HCMV) lytic DNA replication is initiated at the cis-acting oriLyt region and requires six core replication proteins along with UL84 and IE2. Although UL84 is thought to be the replication initiator protein, little is known about its interaction with oriLyt. We have now performed chromatin immuno...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.06.035

    authors： Kagele D,Gao Y,Smallenburg K,Pari GS

    更新日期：2009-09-15 00:00:00

  • Walleye dermal sarcoma virus rv-cyclin inhibits NF-kappaB-dependent transcription.

    abstract：:The retroviral cyclin protein (rv-cyclin) of walleye dermal sarcoma virus contains two known functional domains, a cyclin box motif and a carboxy terminal transcription activation domain (AD). The AD contacts TATA-binding protein-associated factor 9 (TAF9), and this action is necessary for both positive and negative r...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.12.026

    authors： Quackenbush SL,Linton A,Brewster CD,Rovnak J

    更新日期：2009-03-30 00:00:00