Low density contrast agents for x-ray phase contrast imaging: the use of ambient air for x-ray angiography of excised murine liver tissue.

Abstract:

:We report a new preparative method for providing contrast through reduction in electron density that is uniquely suited for propagation-based differential x-ray phase contrast imaging. The method, which results in an air or fluid filled vasculature, makes possible visualization of the smallest microvessels, roughly down to 15 microm, in an excised murine liver, while preserving the tissue for subsequent histological workup. We show the utility of spatial frequency filtering for increasing the visibility of minute features characteristic of phase contrast imaging, and the capability of tomographic reconstruction to reveal microvessel structure and three-dimensional visualization of the sample. The effect of water evaporation from livers during x-ray imaging on the visibility of blood vessels is delineated. The deformed vascular tree in a cancerous murine liver is imaged.

journal_name

Phys Med Biol

authors

Laperle CM,Hamilton TJ,Wintermeyer P,Walker EJ,Shi D,Anastasio MA,Derdak Z,Wands JR,Diebold G,Rose-Petruck C

doi

10.1088/0031-9155/53/23/017

subject

Has Abstract

pub_date

2008-12-07 00:00:00

pages

6911-23

issue

23

eissn

0031-9155

issn

1361-6560

pii

S0031-9155(08)83775-X

journal_volume

53

pub_type

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