Abstract：
:Poly(ADP-ribose) polymerase (PARP) inhibitors are approved for treatment of cancers with BRCA1 or BRCA2 defects. In this study, we prepared and characterized a very long-acting PARP inhibitor. Synthesis of a macromolecular prodrug of talazoparib (TLZ) was achieved by covalent conjugation to a PEG40kDa carrier via a β-eliminative releasable linker. A single injection of the PEG~TLZ conjugate was as effective as ~30 daily oral doses of TLZ in growth suppression of homologous recombination-defective tumors in mouse xenografts. These included the KT-10 Wilms tumor with a PALB2 mutation, the BRCA1-deficient MX-1 triple-negative breast cancer, and the BRCA2-deficient DLD-1 colon cancer; the prodrug did not inhibit an isogenic DLD-1 tumor with wild-type BRCA2. Although the half-life of PEG~TLZ and released TLZ in the mouse was only ~1 day, the exposure of released TLZ from a single safe, effective dose of the prodrug exceeded that of oral TLZ given daily over one month. μPET/CT imaging showed high uptake and prolonged retention of an 89Zr-labeled surrogate of PEG~TLZ in the MX-1 BRCA1-deficient tumor. These data suggest that the long-lasting anti-tumor effect of the prodrug is due to a combination of its long t1/2, the high exposure of TLZ released from the prodrug, increased tumor sensitivity upon continued exposure, and tumor accumulation. Using pharmacokinetic parameters of TLZ in humans, we designed a long-acting PEG~TLZ for humans that may be superior in efficacy to daily oral TLZ and would be useful for treatment of PARP inhibitor-sensitive cancers in which oral medications are not tolerated.
journal_name
Cancer Resjournal_title
Cancer researchauthors
Fontaine SD,Ashley GW,Houghton PJ,Kurmasheva RT,Diolaiti M,Ashworth A,Peer CJ,Nguyen R,Figg WD,Beckford-Vera DR,Santi DVdoi
10.1158/0008-5472.CAN-20-1741subject
Has Abstractpub_date
2020-12-15 00:00:00eissn
0008-5472issn
1538-7445pii
0008-5472.CAN-20-1741pub_type
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