Abstract：
:Isoorientin (ISO), a natural flavonoid compound, has been identified in several plants and its biological activity is determined and the study on lowering uric acid has not been reported. In view of the current status of treatment of hyperuricemia, we evaluated the hypouricemic effects of ISO in vivo and in vitro, and explored the underlying mechanisms. Yeast extract-induced hyperuricemia animal model as well as hypoxanthine and xanthine oxidase (XOD) co-induced high uric acid L-O2 cell model and enzymatic experiments in vitro were selected. The XOD activity and uric acid (UA) level were inhibited after the treatment of ISO in vitro and in vivo. Furthermore, serum creatinine (CRE) and blood urea nitrogen (BUN) levels were also significantly reduced and liver damage was recovered in pathological histology after the ISO administration in hyperuricemia animal model. The results of mechanism illustrated that protein expressions such as XOD, toll-like receptor 4 (TLR4), cathepsin B (CTSB), NLRP3, and its downstream caspase-1 as well as interleukin-18 (IL-18) were markedly downregulated by ISO intervention in vitro and in vivo. Our results suggest that ISO exerts a urate-lowering effect through inhibiting XOD activity and regulating TLR4-NLRP3 inflammasome signal pathway, thus representing a promising candidate therapeutic agent for hyperuricemia. Both animal models and in vitro experiments suggested that ISO may effectively lower uric acid produce. The mechanism might be the inhibition of XOD activity and NLRP3 inflammasome of upregulation.
journal_name
J Nat Medjournal_title
Journal of natural medicinesauthors
An MF,Wang MY,Shen C,Sun ZR,Zhao YL,Wang XJ,Sheng Jdoi
10.1007/s11418-020-01464-zsubject
Has Abstractpub_date
2021-01-01 00:00:00pages
129-141issue
1eissn
1340-3443issn
1861-0293pii
10.1007/s11418-020-01464-zjournal_volume
75pub_type
杂志文章abstract：:Two new diterpenoids, 14α-hydroxy-9α,13α-epoxylabd-5(6)-en-7-on-16,15-olide (1) and 13ξ-hydroxylabd-5(6),8(9)-dien-7-on-16,15-olide (2) along with six known diterpenoids were isolated from the aerial parts of Leonotis leonurus R. Br. These structures were determined on the basis of spectroscopic analyses. Some of the ...
journal_title：Journal of natural medicines
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更新日期：2015-07-01 00:00:00
abstract：:Nardostachys jatamansi (NJ) has been used in the treatment of inflammatory diseases. However, it is not clear how NJ produces anti-inflammatory effects. In the present study, using an experimental model of lipopolysaccharide (LPS)-induced endotoxin shock, the protective effects and mechanisms of action of NJ were inve...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-010-0458-x
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journal_title：Journal of natural medicines
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journal_title：Journal of natural medicines
pub_type： 杂志文章
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更新日期：2019-01-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-020-01457-y
更新日期：2021-01-01 00:00:00
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journal_title：Journal of natural medicines
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更新日期：2009-04-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
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更新日期：2016-01-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
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更新日期：2010-10-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-013-0765-0
更新日期：2014-01-01 00:00:00
abstract：:Aged garlic extract (AGE), a garlic preparation rich in water-soluble cysteinyl moieties, has been reported to have multiple beneficial effects on cardiovascular disease including inhibition of platelet aggregation. However, the mode of AGE action on platelets has not been clear. In this study, we examined the effect ...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-016-1055-4
更新日期：2017-01-01 00:00:00
abstract：:The binding properties of recombinant antibody fragments, such as affinity and specificity, determine their usefulness for therapeutic and analytical applications. Anti-paclitaxel single-chain variable fragment clone 1C2 (anti-PT scFv1C2) was expressed using Escherichia coli cell and Bombyx mori larvae expression syst...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-017-1136-z
更新日期：2018-01-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-010-0429-2
更新日期：2010-10-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-015-0890-z
更新日期：2015-07-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-011-0531-0
更新日期：2011-07-01 00:00:00
abstract：:Oligostilbenoids are a group of natural products derived from the oxidative coupling of C6-C2-C6 units found in some plant families. A structurally diverse chemical pool is produced after the successive regioselective and stereoselective oligomerization of resveratrol. This review describes the current status and know...
journal_title：Journal of natural medicines
pub_type： 杂志文章,评审
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更新日期：2020-09-01 00:00:00
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journal_title：Journal of natural medicines
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journal_title：Journal of natural medicines
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journal_title：Journal of natural medicines
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journal_title：Journal of natural medicines
pub_type： 杂志文章
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journal_title：Journal of natural medicines
pub_type： 杂志文章
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更新日期：2012-04-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-011-0609-8
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journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-012-0707-2
更新日期：2013-07-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
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更新日期：2009-01-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
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更新日期：2012-07-01 00:00:00
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journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-007-0218-8
更新日期：2008-04-01 00:00:00
abstract：:(E)-methyl-4-hydroxy-3,5-dimethoxycinnamate, (E)-methyl-4-hydroxy-3-methoxycinnamate, (E)-methyl-4-hydroxycinnamate, (Z)-methyl-4-hydroxycinnamate, calycanthine, ursolic acid, 2-hydroxy-3-methylanthraquinone, 4-methoxybenzoic acid, 3,4,5-trimethoxybenzoic acid, methyl 4-hydroxybenzoate and allantoin were isolated from...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-008-0227-2
更新日期：2008-07-01 00:00:00
abstract：:Phellodendri Cortex (Obaku in Japanese) and Coptidis Rhizoma (Oren), both of which contain berberine, have been used to prepare the kampo formula orengedokuto to treat inflammatory diseases, including dermatitis, gastric ulcers, and gastritis. These drugs are blended differently in other formulas, such as the use of P...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-017-1107-4
更新日期：2017-10-01 00:00:00
abstract：:Several studies have indicated the involvement of oxidative stress and high glucose-induced cell death in the development of diabetic neuropathy. Satureja khuzestanica has been recommended in the literature as a remedy for the treatment of diabetes, and also contains antioxidant agents. Here, we investigated the possi...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-012-0646-y
更新日期：2013-01-01 00:00:00
