Effects of shakuyakukanzoto and its absorbed components on twitch contractions induced by physiological Ca2+ release in rat skeletal muscle.


:Shakuyakukanzoto (SKT) is a kampo medicine composed of equal proportions of Glycyrrhizae radix (G. radix) and Paeoniae radix (P. radix). A double-blind study reported that SKT significantly ameliorated painful muscle cramp in cirrhosis patients without the typical severe side effects of muscle weakness and central nervous system (CNS) depression. Previous basic studies reported that SKT and its active components induced relaxation by a direct action on skeletal muscle and that SKT did not depress CNS functions; however, why SKT has a lower incidence of muscle weakness remains unknown. In the present study, we investigated which components are absorbed into the blood of rats after a single oral administration of SKT to identify the active components of SKT. We also investigated the effects of SKT and its components on the twitch contraction induced by physiological Ca(2+) release. Our study demonstrated that SKT and five G. radix isolates, which are responsible for the antispasmodic effect of SKT, did not inhibit the twitch contraction in contrast to dantrolene sodium, a direct-acting peripheral muscle relaxant, indicating that the mechanisms of muscle contraction of SKT and dantrolene in skeletal muscle differ. These findings suggest that SKT does not reduce the contractile force in skeletal muscle under physiological conditions, i.e., SKT may have a low risk of causing muscle weakness in clinical use. Considering that most muscle relaxants and anticonvulsants cause various harmful side effects such as weakness and CNS depression, SKT appears to have a benign safety profile.


J Nat Med


Kaifuchi N,Omiya Y,Kushida H,Fukutake M,Nishimura H,Kase Y




Has Abstract


2015-07-01 00:00:00












  • The renoprotective effect of shichimotsukokato on hypertension-induced renal dysfunction in spontaneously hypertensive rats.

    abstract::Antihypertensive treatment is highly important to prevent the progression of chronic kidney disease. Shichimotsukokato (SKT), a traditional Japanese medicine (i.e., Kampo formula), lowered systolic blood pressure (SBP) in experimental animal models of hypertension. However, its mechanism of action has not been fully e...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Ma Y,Fujimoto M,Watari H,Kimura M,Shimada Y

    更新日期:2016-04-01 00:00:00

  • Anti-inflammatory activity of diterpenes from Croton stellatopilosus on LPS-induced RAW264.7 cells.

    abstract::An acyclic diterpene (plaunotol; 1) and two furanoditerpenes (plaunolide, 2 and plaunol E, 3), were isolated from Croton stellatopilosus leaves, and assessed for their inhibitory activity on nitric oxide (NO) production by lipopolysaccharide (LPS)-induced RAW264.7 cells. Plaunotol, plaunolide and plaunol E exhibited i...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Premprasert C,Tewtrakul S,Plubrukarn A,Wungsintaweekul J

    更新日期:2013-01-01 00:00:00

  • Non-alkaloidal composition of Ephedra Herb is influenced by differences in habitats.

    abstract::Ephedra Herb is a crude drug defined as the terrestrial stem of Ephedra sinica, E. intermedia, or E. equisetina. It is often used to treat headaches, bronchial asthma, nasal inflammation, and the common cold. In this study, we isolated characteristic non-alkaloidal constituents from the extracts and identified them in...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Oshima N,Yamashita T,Uchiyama N,Hyuga S,Hyuga M,Yang J,Hakamatsuka T,Hanawa T,Goda Y

    更新日期:2019-01-01 00:00:00

  • Preventive effect of Terminalia bellirica on obesity and metabolic disorders in spontaneously obese type 2 diabetic model mice.

    abstract::Visceral obesity induces insulin resistance and is recognized as an important risk factor for metabolic syndrome (MS). Therefore, inhibition of lipid absorption from the intestine is regarded as an effective way of preventing MS. Terminalia bellirica is extensively used in Ayurvedic medicine in India and neighboring c...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Makihara H,Shimada T,Machida E,Oota M,Nagamine R,Tsubata M,Kinoshita K,Takahashi K,Aburada M

    更新日期:2012-07-01 00:00:00

  • Naringenin suppresses neutrophil infiltration into adipose tissue in high-fat diet-induced obese mice.

    abstract::Recruitment of immune cells to adipose tissue is altered dramatically in obesity, which results in chronic inflammation of the adipose tissue that leads to metabolic disorders, such as insulin resistance and type 2 diabetes mellitus. The regulation of immune cell infiltration into adipose tissue has prophylactic and t...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Tsuhako R,Yoshida H,Sugita C,Kurokawa M

    更新日期:2020-01-01 00:00:00

  • Constituents of the lichen Peltigera dolichorrhiza.

    abstract::A new aromatic derivative, 5-(3,4-dihydroxyphenyl)-3-methylcyclohex-2-enone (1), and three known compounds, peltigerine, solorinine, and phenyl butanoid, were isolated from the MeOH extract of the lichen Peltigera dolichorrhiza (Peltigeraceae). Their structures were determined by 1D and 2D nuclear magnetic resonance a...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Yoshikawa K,Kunioka E,Kokudo N,Hashimoto T

    更新日期:2010-07-01 00:00:00

  • Establishment of a methodology for identifying Paeoniae Radix based on metallomic analysis.

    abstract::We aimed to establish a methodology for identifying Paeonia samples based on metallomic analysis. We prepared 66 batches of samples (16 batches of crude drugs and 50 batches of cultivars, comprising 64 batches of Paeonia lactiflora and 2 batches of P. veitchii) collected from Japan and China (Inner Mongolia and elsewh...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Shimada K,Nakamura Y,Kawase M,Komatsu K,Saito T,Takahashi K

    更新日期:2014-04-01 00:00:00

  • Sesquiterpenes from Curcuma comosa.

    abstract::From the dried rhizomes of Curcuma comosa cultivating in Thailand, 26 known sesquiterpenes were isolated: zederone, zederone epoxide, furanodienone, isofuranodienone, 1(10)Z,4Z-furanodiene-6-one, glechomanolide, dehydrocurdione, neocurdione, curdione, 7 alpha-hydroxyneocurdione, 7 beta-hydroxycurdione, germacrone-1(10...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Qu Y,Xu F,Nakamura S,Matsuda H,Pongpiriyadacha Y,Wu L,Yoshikawa M

    更新日期:2009-01-01 00:00:00

  • Pharmacological explanation for the medicinal use of Juniperus excelsa in hyperactive gastrointestinal and respiratory disorders.

    abstract::Crude extract of Juniperus excelsa (JeExt), which tested positive for the presence of anthraquinone, flavonoids, saponins, sterols, terpenes and tannin, exhibited a protective effect against castor oil-induced diarrhoea in mice at 100-1000 mg/kg. In rabbit jejunum preparations, JeExt (0.01-1.0 mg/mL) caused relaxation...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Khan M,Khan AU,Najeeb-ur-Rehman,Gilani AH

    更新日期:2012-04-01 00:00:00

  • Coumarins from the roots of Ligusticum multivittatum.

    abstract::Five new coumarins, (+)-peujaponisin (1) and multivittans A-D (2-5), were isolated from the roots of Ligusticum multivittatum Franch., and their structures were established by spectral means. ...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Kondo T,Taniguchi M,Shibano M,Wang NH,Baba K

    更新日期:2008-01-01 00:00:00

  • Study on selected trace elements and heavy metals in some popular medicinal plants from Sudan.

    abstract::This study reports on the determination of 11 elements in 33 medicinal plants from Sudan and discusses a possible correlation between their curative effects and their trace elements content. Further, a possible accumulation of adverse heavy metals could be excluded. A total of 11 elements (cadmium, lead, mercury, tin,...

    journal_title:Journal of natural medicines

    pub_type: 信件


    authors: Ebrahim AM,Eltayeb MH,Khalid H,Mohamed H,Abdalla W,Grill P,Michalke B

    更新日期:2012-10-01 00:00:00

  • Possible attenuation of nitric oxide expression in anti-inflammatory effect of Ziziphus jujuba in rat.

    abstract::The hydroalcoholic extract of fruits of Ziziphus jujuba (ZJ) was investigated for its anti-inflammatory effect using acute and chronic models of inflammation in rat. Wistar albino rats of either sex were employed in the present study (n = 6). Acute inflammation was induced by subplantar administration of carrageenan (...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Goyal R,Sharma PL,Singh M

    更新日期:2011-07-01 00:00:00

  • Resveratrol oligomer structure in Dipterocarpaceaeous plants.

    abstract::Oligostilbenoids are a group of natural products derived from the oxidative coupling of C6-C2-C6 units found in some plant families. A structurally diverse chemical pool is produced after the successive regioselective and stereoselective oligomerization of resveratrol. This review describes the current status and know...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章,评审


    authors: Ito T

    更新日期:2020-09-01 00:00:00

  • The potential benefits of a garlic and hydrochlorothiazide combination as antihypertensive and cardioprotective in rats.

    abstract::The purpose of the present study was to investigate the protective effects of combined therapy of garlic homogenate and hydrochlorothiazide (HCTZ) in animals with hypertension and myocardial damage. Three weeks of high fructose (10% w/v) in fluid to albino rats resulted in hypertension. They were subsequently administ...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Asdaq SM,Inamdar MN

    更新日期:2011-01-01 00:00:00

  • Composition of the endophytic filamentous fungi isolated from the tea plant Camellia sinensis.

    abstract::It has been found by ribosomal DNA analysis that the endophytic filamentous fungi isolated from the tea plant Camellia sinensis (Theaceae) are composed of six groups; one Fusarium sp., one Penicillium sp., two Schizophyllum sp., and two Diaporthe sp.. ...

    journal_title:Journal of natural medicines

    pub_type: 社论


    authors: Agusta A,Ohashi K,Shibuya H

    更新日期:2006-07-01 00:00:00

  • Antithrombosis activity of protocatechuic and shikimic acids from functional plant Pinus densiflora Sieb. et Zucc needles.

    abstract::Pine needle extract (PE) and fermented pine needle extract (FPE) have been reported to show various biological and pharmacological activities such as antioxidant, anti-inflammatory, anti-bacterial, anti-cholesterol, gastrointestinal motility control, and fibrinolytic effect. The aims of our research were to isolate fi...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Park J,Lee B,Choi H,Kim W,Kim HJ,Cheong H

    更新日期:2016-07-01 00:00:00

  • Biological effects of Ocimum gratissimum L. are due to synergic action among multiple compounds present in essential oil.

    abstract::Species of genus Ocimum (Lamiaceae) are economically important due to their essential oils and utilization as medicine for many disorders, including the central nervous system. A previous study showed seasonal variations in chemical profile and in central nervous system activities of essential oil from Ocimum gratissi...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Galindo LA,Pultrini Ade M,Costa M

    更新日期:2010-10-01 00:00:00

  • Aged garlic extract suppresses platelet aggregation by changing the functional property of platelets.

    abstract::Aged garlic extract (AGE), a garlic preparation rich in water-soluble cysteinyl moieties, has been reported to have multiple beneficial effects on cardiovascular disease including inhibition of platelet aggregation. However, the mode of AGE action on platelets has not been clear. In this study, we examined the effect ...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Morihara N,Hino A

    更新日期:2017-01-01 00:00:00

  • Effect of phenylalanine on Taxol production and antioxidant activity of extracts of suspension-cultured hazel (Corylus avellana L.) cells.

    abstract::Taxol is produced by a few microorganisms and plants such as yew (Taxus sp.). Recent researches have shown that hazel (Corylus avellana L.) is also able to produce Taxol. In the present study, effects of different concentrations of phenylalanine (Phe) on the production of Taxol, antioxidant activity, and cytotoxic eff...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Bemani E,Ghanati F,Rezaei A,Jamshidi M

    更新日期:2013-07-01 00:00:00

  • Mangostanaxanthones III and IV: advanced glycation end-product inhibitors from the pericarp of Garcinia mangostana.

    abstract::Advanced glycation end-products (AGEs) are associated with a non-enzymatic reaction between the amino group of a protein and the carbonyl group of a sugar during hyperglycemia. The precipitation of AGEs in different tissues leads to many complications, such as endothelial dysfunction, cardiovascular complications, ath...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Abdallah HM,El-Bassossy HM,Mohamed GA,El-Halawany AM,Alshali KZ,Banjar ZM

    更新日期:2017-01-01 00:00:00

  • Attenuating effect of Fufang Xueshuantong Capsule on kidney function in diabetic nephropathy model.

    abstract::Fufang Xueshuantong Capsule (FXST) can reduce urinary albumin and whole blood viscosity in early diabetic nephropathy (DN) patients. This research aimed to investigate the effect of FXST on kidney function in DN rats and to identify the underlying molecular mechanisms. We performed Illumina RatRef-12 Expression BeadCh...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Zhang Q,Xiao X,Li M,Li W,Yu M,Zhang H,Sun X,Mao L,Xiang H

    更新日期:2013-01-01 00:00:00

  • Identification of anti-inflammatory constituents in Phellodendri Cortex and Coptidis Rhizoma by monitoring the suppression of nitric oxide production.

    abstract::Phellodendri Cortex (Obaku in Japanese) and Coptidis Rhizoma (Oren), both of which contain berberine, have been used to prepare the kampo formula orengedokuto to treat inflammatory diseases, including dermatitis, gastric ulcers, and gastritis. These drugs are blended differently in other formulas, such as the use of P...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Fujii A,Okuyama T,Wakame K,Okumura T,Ikeya Y,Nishizawa M

    更新日期:2017-10-01 00:00:00

  • Cytotoxic abietane and kaurane diterpenoids from Celastrus orbiculatus.

    abstract::Celastrus orbiculatus is a medicinal plant belonging to the Celastraceae family. In this survey on the secondary metabolites of plants for obtaining antitumor substances, the chemical constituents of the stems of C. orbiculatus were investigated. Nortriptonoterpene (1), a new C19-norabietane diterpenoid, together with...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Jiang W,Shan TZ,Xu JJ,Chen WJ,Miao L,Lv MY,Tao L,Liu YQ

    更新日期:2019-09-01 00:00:00

  • Inhibitory effects of devil's claw (secondary root of Harpagophytum procumbens) extract and harpagoside on cytokine production in mouse macrophages.

    abstract::Successive oral administration (50 mg/kg) of a 50% ethanolic extract (HP-ext) of devil's claw, the secondary root of Harpagophytum procumbens, showed a significant anti-inflammatory effect in the rat adjuvant-induced chronic arthritis model. HP-ext dose-dependently suppressed the lipopolysaccharide (LPS)-induced produ...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Inaba K,Murata K,Naruto S,Matsuda H

    更新日期:2010-04-01 00:00:00

  • Chemical constituents from leaves of Palicourea coriacea (Rubiaceae).

    abstract::(E)-methyl-4-hydroxy-3,5-dimethoxycinnamate, (E)-methyl-4-hydroxy-3-methoxycinnamate, (E)-methyl-4-hydroxycinnamate, (Z)-methyl-4-hydroxycinnamate, calycanthine, ursolic acid, 2-hydroxy-3-methylanthraquinone, 4-methoxybenzoic acid, 3,4,5-trimethoxybenzoic acid, methyl 4-hydroxybenzoate and allantoin were isolated from...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: da Silva VC,de Carvalho MG,Alves AN

    更新日期:2008-07-01 00:00:00

  • Four new cucurbitane-type triterpenes from Momordica charantia L. with their cytotoxic activities and protective effects on H2O2-damaged pancreatic cells.

    abstract::Four new cucurbitane-type triterpenes were isolated from the fruit of Momordica charantia L. The structures of the new compounds were identified based on HR-ESI-MS and 1D- and 2D-NMR spectroscopic methods. The cytotoxicity of the isolated compounds was evaluated using three human cancer cell lines, HeLa, Caco2, and U8...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Yue J,Sun Y,Xu J,Zhang X,Zhao Y

    更新日期:2020-01-01 00:00:00

  • The protective potential of Yucca schidigera (Sarsaponin 30) against nitrite-induced oxidative stress in rats.

    abstract::The present study was designed to determine the protective effects of Yucca schidigera (Ys) against oxidative damage induced by acute nitrite intoxication as well as the histopathological evaluation of Ys in rats. The rats were divided into three groups each containing 12 rats: control (C); nitrite intoxication (N); Y...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Cigerci IH,Fidan AF,Konuk M,Yuksel H,Kucukkurt I,Eryavuz A,Sozbilir NB

    更新日期:2009-07-01 00:00:00

  • Satureja khuzestanica attenuates apoptosis in hyperglycemic PC12 cells and spinal cord of diabetic rats.

    abstract::Several studies have indicated the involvement of oxidative stress and high glucose-induced cell death in the development of diabetic neuropathy. Satureja khuzestanica has been recommended in the literature as a remedy for the treatment of diabetes, and also contains antioxidant agents. Here, we investigated the possi...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Kaeidi A,Esmaeili-Mahani S,Abbasnejad M,Sheibani V,Rasoulian B,Hajializadeh Z,Pasban-Aliabadi H

    更新日期:2013-01-01 00:00:00

  • A new diterpenoid from the leaves of Clerodendron trichotomum.

    abstract::A new diterpenoid was isolated from the leaves of Clerodendron trichotomumThunb. (Verbenaceae) along with one each of a known diterpenoid, phenylethanoid glycoside, and sterol and two known flavonoids. Their chemical structures were characterized on the basis of spectroscopic data and X-ray analysis. In addition, thei...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Ono M,Furusawa C,Matsumura K,Noguchi S,Yasuda S,Okawa M,Kinjo J,Eto M,Yamaguchi K,Yoshimitsu H,Nohara T

    更新日期:2013-04-01 00:00:00

  • New phenanthrene and 9, 10-dihydrophenanthrene derivatives from the stems of Dendrobium officinale with their cytotoxic activities.

    abstract::Two new phenanthrene and 9, 10-dihydrophenanthrene derivatives (1-2) with six known congeners (3-8) were isolated from the extraction of stems of Dendrobium officinale. Compounds 1 and 2 were based on carbon skeleton in which phenanthrene and 9, 10-dihydrophenanthrene moiety were linked with a phenylpropane unit throu...

    journal_title:Journal of natural medicines

    pub_type: 杂志文章


    authors: Zhao GY,Deng BW,Zhang CY,Cui YD,Bi JY,Zhang GG

    更新日期:2018-01-01 00:00:00