Metabonomic characterization of aging and investigation on the anti-aging effects of total flavones of Epimedium.

abstract：:Protein-protein interactions are important for the majority of biological processes. A significant number of computational methods have been developed to predict protein-protein interactions using protein sequence, structural and genomic data. Vast experimental data is publicly available on the Internet, but it is sca...

journal_title：Molecular bioSystems

authors： Saha I,Zubek J,Klingström T,Forsberg S,Wikander J,Kierczak M,Maulik U,Plewczynski D

更新日期：2014-04-01 00:00:00

abstract：:Cervical cancer is a clinical and pathological heterogeneity disease, which requires different types of treatments and leads to a variety of outcomes. In clinical practice, only some patients benefit from chemotherapy treatment. Identifying patients who will be responsive to chemotherapy could increase their survival ...

journal_title：Molecular bioSystems

authors： Hou Y,Yin M,Sun F,Zhang T,Zhou X,Li H,Zheng J,Chen X,Li C,Ning X,Lou G,Li K

更新日期：2014-08-01 00:00:00

abstract：:The identification of genetic markers (e.g. genes, pathways and subnetworks) for cancer has been one of the most challenging research areas in recent years. A subset of these studies attempt to analyze genome-wide expression profiles to identify markers with high reliability and reusability across independent whole-tr...

journal_title：Molecular bioSystems

authors： Farahmand S,Goliaei S,Ansari-Pour N,Razaghi-Moghadam Z

更新日期：2016-03-01 00:00:00

abstract：:Genome sequencing technology has generated a vast amount of genomic and epigenomic data, and has provided us a great opportunity to study gene functions on a global scale from an epigenomic view. In the last decade, network-based studies, such as those based on PPI networks and co-expression networks, have shown good ...

journal_title：Molecular bioSystems

authors： Li W,Wang M,Sun J,Wang Y,Jiang R

更新日期：2017-10-24 00:00:00

abstract：:The pro-inflammatory cytokines interleukin 1 (IL-1) and 6 (IL-6) are crucially involved in the regulation of a multitude of physiological processes, in particular coordinating the immune response upon bacterial infection and tissue injury. Both interleukins induce complex signalling cascades and trigger the production...

journal_title：Molecular bioSystems

authors： Ryll A,Samaga R,Schaper F,Alexopoulos LG,Klamt S

更新日期：2011-12-01 00:00:00

abstract：:The significant increase in the demand for purified protein for crystallization and structural studies has made necessary the development of multi-sample methods for identifying solution conditions that affect protein stability and aggregation. Conditions that stabilize proteins can improve protein purification and cr...

journal_title：Molecular bioSystems

authors： Senisterra GA,Finerty PJ Jr

更新日期：2009-03-01 00:00:00

abstract：:Sponge metagenomes are accessible genetic sources containing genes and gene clusters responsible for the biosynthesis of sponge-derived bioactive natural products. In this study, we obtained the clone pDC112, producing turbomycin A and 2,2-di(3-indolyl)-3-indolone, based on the functional screening of the metagenome l...

journal_title：Molecular bioSystems

authors： Takeshige Y,Egami Y,Wakimoto T,Abe I

更新日期：2015-05-01 00:00:00

abstract：:Conventional methods for detecting single nucleotide polymorphisms (SNPs), including direct DNA sequencing, pyrosequencing, and melting curve analysis, are to a great extent limited by their requirement for particular detection instruments. To overcome this limitation, we established a novel SNP detection technique ut...

journal_title：Molecular bioSystems

authors： Imai K,Ogai Y,Nishizawa D,Kasai S,Ikeda K,Koga H

更新日期：2007-08-01 00:00:00

abstract：:The investigation of the structure and dynamics of signal transduction systems through data-based mathematical models in ordinary differential equations or other paradigms has proven to be a successful approach in recent times. Extending this concept, we here analysed the use of kinetic models based on power-law terms...

journal_title：Molecular bioSystems

authors： Vera J,Rath O,Balsa-Canto E,Banga JR,Kolch W,Wolkenhauer O

更新日期：2010-11-01 00:00:00

abstract：:Cellular heterogeneity and stochastic fluctuation play key roles in biological processes. Single molecule approaches have the key advantage of avoiding ensemble averaging, allowing the observation of transient intermediates and heterogeneity (both static and dynamic). Thus they have revolutionised the way many biologi...

journal_title：Molecular bioSystems

authors： Ying L

更新日期：2007-06-01 00:00:00

abstract：:The non-classical zinc finger protein, Neural Zinc Finger Factor-1, contains six Cys2His2Cys domains. All three cysteines and the second histidine directly bind Zn(II). Using a combination of mutagenesis, metal coordination and DNA binding studies, we report that the first histidine is involved in a functionally impor...

journal_title：Molecular bioSystems

authors： Besold AN,Amick DL,Michel SL

更新日期：2014-07-01 00:00:00

abstract：:Abundant information about the nucleotide sequence of the human genome has become readily available and it is now necessary to develop methods for the identification of genes that are involved in important cellular, developmental and disease-related processes. Identification methods based on the activities of hammerhe...

journal_title：Molecular bioSystems

authors： Sano M,Kato Y,Taira K

更新日期：2005-05-01 00:00:00

abstract：:Low transfection efficiency and inactivation by serum are the major drawbacks for cationic polymers when used as non-viral gene vectors. Here, a series of N-succinyl-chitosan-graft-polyethylenimine (NSC-g-PEI) copolymers with different compositions were synthesized through grafting low molecular weight PEI (800 Da) to...

journal_title：Molecular bioSystems

authors： Lu B,Sun YX,Li YQ,Zhang XZ,Zhuo RX

更新日期：2009-06-01 00:00:00

abstract：:Plant stress hormones, such as jasmonates (JAs) and ethylene (ET) are essential in plant defence against stress conditions. JAs are used in cosmetics and food flavouring, and the recently demonstrated anti-cancer activity of JAs highlights their potential in health protection. It reinforces the need for a better under...

journal_title：Molecular bioSystems

authors： Schweighofer A,Meskiene I

更新日期：2008-08-01 00:00:00

abstract：:The search for molecules with activity against Mycobacterium tuberculosis (Mtb) is employing many approaches in parallel including high throughput screening and computational methods. We have developed a database (CDD TB) to capture public and private Mtb data while enabling data mining and collaborations with other r...

journal_title：Molecular bioSystems

authors： Ekins S,Bradford J,Dole K,Spektor A,Gregory K,Blondeau D,Hohman M,Bunin BA

更新日期：2010-05-01 00:00:00

abstract：:Glucocorticoids (GCs) have been proved to be an important pathogenic factor of some neuropsychiatric disorders. Usually, a classical injury model based on corticosterone-induced cytotoxicity of differentiated rat pheochromocytoma (PC12) cells was used to stimulate the state of GC damage of hippocampal neurons and inve...

journal_title：Molecular bioSystems

authors： Zhang H,Zheng H,Zhao G,Tang C,Lu S,Cheng B,Wu F,Wei J,Liang Y,Ruan J,Song H,Su Z

更新日期：2016-03-01 00:00:00

abstract：:A major mode of signal transduction in bacteria is the two-component system, which involves phosphorylation of an output-generating receiver protein by a signal-sensing histidine kinase. This differs from the more common one-component system--where both signal sensing and output generation are performed by the same pr...

journal_title：Molecular bioSystems

authors： Seshasayee AS,Luscombe NM

更新日期：2011-11-01 00:00:00

abstract：:Single molecule observation of fluorescence resonance energy transfer can be used to provide insight into the structure and dynamics of proteins. Using a straightforward triple-colour labelling strategy, we present a measurement and analysis scheme that can simultaneously study multiple regions within single intrinsic...

journal_title：Molecular bioSystems

authors： Milles S,Koehler C,Gambin Y,Deniz AA,Lemke EA

更新日期：2012-10-01 00:00:00

abstract：:Tankyrases (TNKS), key transmitters in the Wnt signaling pathway which is very conservative in evolution, are vital targets as they are overexpressed widely in many cancers. In this work, 5 inhibitors with novel structures have been discovered and validated using the ligand-based (pharmacophore) virtual screening, doc...

journal_title：Molecular bioSystems

authors： Pu Y,Zhang S,Chang Z,Zhang Y,Wang D,Zhang L,Li Y,Zuo Z

更新日期：2017-01-31 00:00:00

abstract：:Precise quantification of endogenous protein-protein interactions across live cells would be a major boon to biology. Such precise measurement is theoretically possible with fluorescence lifetime imaging microscopy (FLIM) but requires first properly addressing multiple biological, instrumental, statistical, and photop...

journal_title：Molecular bioSystems

authors： Walther KA,Papke B,Sinn MB,Michel K,Kinkhabwala A

更新日期：2011-02-01 00:00:00

abstract：:Primarily used for medicinal purposes in the past, biologically active small molecules have been increasingly employed to explore complex biological processes in the era of "chemical genetics". Since the contributions of this small molecule approach to biology have been extensive, we limit the focus of our review to t...

journal_title：Molecular bioSystems

authors： Wehenkel M,Hong JT,Kim KB

更新日期：2008-04-01 00:00:00

abstract：:The chemical composition of the cervical mucus (CM), its physical characteristics and the volume of secretion change cyclically throughout the menstrual cycle. The aim of this study was to identify the constitutive protein composition of CM of fertile women and the changes in the CM proteome throughout the menstrual c...

journal_title：Molecular bioSystems

authors： Grande G,Milardi D,Vincenzoni F,Pompa G,Biscione A,Astorri AL,Fruscella E,De Luca A,Messana I,Castagnola M,Marana R

更新日期：2015-06-01 00:00:00

abstract：:Plant aquaporins belong to a large superfamily of conserved proteins called the major intrinsic proteins (MIPs). There is limited information about the diversity of MIPs and their water transport capacity in broccoli (Brassica oleracea) plants. In this study, the cDNAs of isoforms of Plasma Membrane Intrinsic Proteins...

journal_title：Molecular bioSystems

authors： Muries B,Faize M,Carvajal M,Martínez-Ballesta Mdel C

更新日期：2011-04-01 00:00:00

abstract：:We report the successful application of selective excitation sculptured TOCSY NMR (SXS-TOCSY) to identify individual solution components from a heterogeneous system using selectively acquired (1)H NMR spin system patterns. SXS-TOCSY application is illustrated by detection of the simultaneous presence of poly-beta-(1,6...

journal_title：Molecular bioSystems

authors： Wagstaff JL,Sadovskaya I,Vinogradov E,Jabbouri S,Howard MJ

更新日期：2008-02-01 00:00:00

abstract：:High levels of DNA and RNA released by apoptotic and necrotic cells circulate in the blood of cancer patients. In the present study we determined the applicability of the quantification of nucleic acids and their genetic alterations as minimally invasive tool for breast cancer screening. The relative concentrations of...

journal_title：Molecular bioSystems

authors： Schwarzenbach H,Müller V,Milde-Langosch K,Steinbach B,Pantel K

更新日期：2011-10-01 00:00:00

abstract：:We recently reported a chemical genetic method for generating bivalent inhibitors of protein kinases. This method relies on the use of the DNA repair enzyme O(6)-alkylguanine-DNA alkyltransferase (AGT) to display an ATP-competitive inhibitor and a ligand that targets a secondary binding domain. With this method potent...

journal_title：Molecular bioSystems

authors： Hill ZB,Perera BG,Maly DJ

更新日期：2011-02-01 00:00:00

abstract：:The adaptation of the cellular network to functional changes, timing and patterning of gene expression is regulated by binding of transcription factors to gene regulatory elements, which in turn depends on co-occurring histone modifications. These two layers influence each other, enabling a further level of regulatory...

journal_title：Molecular bioSystems

authors： Schueler M,Zhang Q,Schlesinger J,Tönjes M,Sperling SR

更新日期：2012-02-01 00:00:00

abstract：:Structural insight into DNA quadruplex structures formed by oligodeoxyribonucleotides 3'TG5'-5'GGGT3' (QS55) and 5'TG3'-3'GGGT5' (QS33) is presented. NMR analysis reveals that QS33 forms a parallel-like four-fold symmetric quadruplex, while QS55 possesses a two-fold symmetry and is characterized by a tetrameric antipa...

journal_title：Molecular bioSystems

authors： Galeone A,Mayol L,Virgilio A,Virno A,Randazzo A

更新日期：2008-05-01 00:00:00

abstract：:Blockage of the interactions between immunologic checkpoint protein PD-1 and its ligand PD-L1 showed efficacy for cancer treatment. X-ray structures have captured static conformational snapshots of PD-1 and revealed that the CC' loop adopts an open conformation in the apo-protein but turns into a closed form and inter...

journal_title：Molecular bioSystems

authors： Liu W,Huang B,Kuang Y,Liu G

更新日期：2017-05-02 00:00:00

abstract：:The selective inhibition for JAK2 over the other JAK family kinases (JAK1, JAK3 and TYK2) has shared an immense challenge due to high conservatism. In this paper, the highly JAK2 selective mechanism of the thienopyridine derivative was identified at the molecular level, based on insights into the inhibitory effects of...

journal_title：Molecular bioSystems

authors： Li JJ,Cheng P,Tu J,Zhai HL,Zhang XY

更新日期：2016-02-01 00:00:00