Abstract:
:Cells from newborn rat hindlimb show greatly enhanced myogenicity when grown on surfaces coated with poly-L-lysine followed by laminin (PLL/Lam) instead of the collagens routinely used. Coating with poly-L-lysine (PLL) alone or with PLL followed by collagen does not enhance myogenicity. Both myogenic and nonmyogenic cells, as distinguished by a monoclonal antibody specific for desmin, attach equally well to collagen- and laminin-coated surfaces, but there is a two- to five-fold increase in the number of myogenic cells on PLL/Lam by 72 hr, followed by increased myotube formation. To determine whether this increase in myogenic cells was a consequence of a selective increase in proliferation on PLL/Lam, incorporation of 5-bromodeoxyuridine into DNA followed by labeling with anti-BrdUrd antibody was used as an index of cell proliferation. The results indicate that desmin is expressed in replicating rat myoblasts, and that replication of myogenic cells is greatly enhanced on laminin compared to collagen. The rate of replication of nonmyogenic cells is the same on both substrates. Addition of 10 micrograms/ml laminin to the medium of cells seeded on PLL or collagen has no effect on myogenicity. We conclude that a laminin substrate enhances skeletal myogenesis in vitro by promoting selectively the replication of myoblasts. Cultures prepared from fetuses at 17 and 19 days gestation also show enhanced myogenicity when grown on PLL/Lam, while those from 15-day fetuses do not. Growth and development of fetal myoblasts on collagen were very poor, whereas myoblasts from the newborn rat do proliferate and differentiate on this substrate. Thus myogenic cells at different stages of fetal and neonatal development may require and respond to different extracellular environments. Myotube formation in the E63 clone of L8 rat myoblasts is inhibited by PLL/Lam.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Foster RF,Thompson JM,Kaufman SJdoi
10.1016/0012-1606(87)90327-7subject
Has Abstractpub_date
1987-07-01 00:00:00pages
11-20issue
1eissn
0012-1606issn
1095-564Xpii
0012-1606(87)90327-7journal_volume
122pub_type
杂志文章abstract::Nonmuscle myosin-II is a key motor protein that drives cell shape change and cell movement. Here, we analyze the function of nonmuscle myosin-II during Drosophila embryonic myogenesis. We find that nonmuscle myosin-II and the adhesion molecule, PS2 integrin, colocalize at the developing muscle termini. In the paradigm...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0452
更新日期:2001-11-15 00:00:00
abstract::TGF-beta (TGFβ) family mediated Smad signaling is involved in mesoderm and endoderm specifications, left-right asymmetry formation and neural tube development. The TGFβ1/2/3 and Activin/Nodal signal transduction cascades culminate with activation of SMAD2 and/or SMAD3 transcription factors and their overactivation are...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.09.025
更新日期:2014-12-01 00:00:00
abstract::In warm-blooded vertebrates it is generally accepted that after early stages of development new neurons are not added to the retina. Contrary to this belief, we show here that hatched chickens have a zone of proliferating cells at the peripheral margin of the retina, similar to that of fish and amphibians. We found th...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9640
更新日期:2000-04-15 00:00:00
abstract::The distribution of the catalytic and regulatory subunits of the cAMP-dependent protein kinase between cytoplasm and nucleus was determined during the development of Dictyostelium discoideum. In vegetative amoebae approximately 2% of the subunits were in the nucleus. During development there was an approximately 5-fol...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90221-6
更新日期:1986-05-01 00:00:00
abstract::The C-X-C motif ligand 14 (CXCL14) is a recently discovered chemokine that is highly conserved in vertebrates and expressed in various embryonic and adult tissues. CXCL14 signaling has been implicated to function as an antiangiogenic and anticancer agent in adults. However, its function during development is unknown. ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.01.008
更新日期:2017-03-01 00:00:00
abstract::Complement components have been implicated in a wide variety of functions including neurogenesis, proliferation, cell migration, differentiation, cancer, and more recently early development and regeneration. Following our initial observations indicating that C3a/C3aR signaling induces chick retina regeneration, we ana...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.05.019
更新日期:2017-08-01 00:00:00
abstract::Pax3/7 paired homeodomain transcription factors are important markers of muscle stem cells. Pax3 is required upstream of myod for lateral dermomyotomal cells in the amniote somite to form particular muscle cells. Later Pax3/7-dependent cells generate satellite cells and most body muscle. Here we analyse early myogenes...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.10.009
更新日期:2007-02-15 00:00:00
abstract::Oocyte maturation (meiosis reinitiation) in starfish is induced by the natural hormone 1-methyladenine (1-MeAde). Oocytes of Evasterias troschelii contain 0.43 pmole cyclic AMP/mg protein and 0.47 pmole cyclic GMP/mg protein. Upon stimulation by 1-MeAde the oocytes undergo a moderate (10-30%) decrease in their cAMP co...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90166-7
更新日期:1987-06-01 00:00:00
abstract::Directed cell movement is integral to both embryogenesis and hematopoiesis. In the adult, the chemokine family of secreted proteins signals migration of hematopoietic cells through G-coupled chemokine receptors. We detected embryonic expression of chemokine receptor messages by RT-PCR with degenerate primers at embryo...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9405
更新日期:1999-09-15 00:00:00
abstract::Nematodes provide a useful experimental system with which to investigate the evolution of development at the cellular, genetic, and molecular levels. Building on an understanding of vulval development in Caenorhabditis elegans, analysis of vulval development has been extended to a number of other species in three fami...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1006/dbio.1996.0035
更新日期:1996-02-01 00:00:00
abstract::Hedgehog (Hh) signaling is thought to occur in primary cilia, but the molecular basis of Gli2 and Gli3 activation by Hh signaling in cilia is unknown. Similarly, how ciliary gene mutations result in reduced Gli3 processing that generates a repressor is also not clear. Here we show that Hh signaling inhibits Gli2 and G...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.06.035
更新日期:2017-09-01 00:00:00
abstract::A fundamental issue during nervous system development is how individual cells are formed from the undefined precursors. Differentiated neurons and glia, two major cell types mediating neuronal function, are acquired from immature precursors via a series of explicit controls exerted by transcription factors such as pro...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2012.07.018
更新日期:2012-10-01 00:00:00
abstract::In Xenopus gastrulation, the involuting mesodermal and non-involuting ectodermal cells remain separated from each other, undergoing convergent extension. Here, we show that Eph-ephrin signaling is crucial for the tissue separation and convergence during gastrulation. The loss of EphA4 function results in aberrant gast...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.12.012
更新日期:2011-02-15 00:00:00
abstract::Expression of cardiac troponin I (TnIcardiac) and slow skeletal troponin I (TnIslow) genes was analyzed at the mRNA and protein level in the developing rat heart. TnIslow mRNA was detectable by in situ hybridization in the embryonic cardiac tube as early as the 13-somite stage (Embryonic Day 10). In contrast, TnIcardi...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1074
更新日期:1993-03-01 00:00:00
abstract::Mutations at the murine steel (Sl) locus encoding the ligand for the c-kit receptor result in defects in gametogenesis, hematopoiesis, and melanogenesis. Steel Panda (Slpan) is an allele at the Sl locus obtained by an X-ray mutagenesis protocol. Slpan/Slpan homozygotes are mildly anemic black-eyed whites with pigmente...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1115
更新日期:1993-05-01 00:00:00
abstract::Inverse gradients of transcriptional repressors antagonize the transcriptional effector response to morphogens. However, the role of such inverse regulation might not manifest solely from lack of repressors. Sonic hedgehog (Shh) patterns the forebrain by being expressed ventrally; however, absence of antagonizing Gli3...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.03.011
更新日期:2019-06-01 00:00:00
abstract::In the developing retina, as in other regions of the CNS, neural progenitors give rise to individual cell types during discrete temporal windows. Pax6 is expressed in retinal progenitor cells (RPCs) throughout the course of retinogenesis, and has been shown to be required during early retinogenesis for generation of m...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.09.030
更新日期:2017-12-01 00:00:00
abstract::Fertilization-related sperm mitochondrial movement occurs at a rate comparable to other actin-myosin-driven movements and is inhibited by cytochalasin B and N-ethyl maleimide in Ascidia ceratodes sperm. F-actin was demonstrated in the tails and mitochondria using NBD-phallacidin fluorescence. Both actin and myosin wer...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90229-x
更新日期:1984-12-01 00:00:00
abstract::In this report we describe the development of a sensitive assay for gene expression in zebrafish embryos using beta-lactamase as a reporter gene. We show that injection of a green fluorescent substrate for beta-lactamase allows the detection of reporter gene expression in live embryos. The beta-lactamase enzyme cataly...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8999
更新日期:1998-11-15 00:00:00
abstract::When a posterior fragment of the chick's marginal zone (PM) was exchanged with equal sized lateral marginal zone fragment (LM), of the same blastoderm, its capacity to initiate an ectopic primitive streak (PS) was found to be both size and stage dependent. Good correlation was demonstrated between the areas of PM frag...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90091-2
更新日期:1989-07-01 00:00:00
abstract::Vitelline coats (VCs) of Phallusia mammillata were isolated and purified following homogenization of live eggs in order to investigate the molecular basis of sperm-egg recognition. Clean VCs were partly solubilized by sonication in H2O and the soluble fraction (SFVC), derived from the outer surface of VCs, was used fo...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90272-5
更新日期:1991-12-01 00:00:00
abstract::Hair shafts are produced from stem cells located in the bulge. Our knowledge of the genetic pathways regulating cell fate acquisition in the immediate descendents of these stem cells, and fate maintenance in their committed progeny, is still incomplete. One pathway involved in fate maintenance within the hair matrix i...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.11.021
更新日期:2009-02-15 00:00:00
abstract::In normal development the veg1 tier of the sixth cleavage Strongylocentrotus purpuratus embryo contributes progeny to both ectodermal lineages and portions of the archenteron. Treatment with 18 mM LiCl specifically affects this lineage allocation, reducing or eliminating the veg1 contribution to ectoderm. Less frequen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8615
更新日期:1997-07-15 00:00:00
abstract::Animal development is controlled by gene regulation networks that are composed of sequence-specific transcription factors (TF) and cell signaling molecules (ST). Although housekeeping genes have been reported to show clustering in the animal genomes, whether the genes comprising a given regulatory network are physical...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.01.009
更新日期:2008-04-15 00:00:00
abstract::We have cloned a partial cDNA of chicken glial cell line-derived neurotrophic factor (GDNF) and systematically examined its expression pattern as well as that of GDNF-binding components (GDNF family receptor alpha-1 and 2: GFRalpha-1 and 2) and a common signal transduction receptor (c-ret protooncogene: RET) during ve...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9543
更新日期:2000-01-01 00:00:00
abstract::Vascular smooth muscle of the head derives from neural crest, but developmental mechanisms and early transcriptional drivers of the vSMC lineage are not well characterized. We find that in early development, the transcription factor foxc1b is expressed in mesenchymal cells that associate with the vascular endothelium....
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.06.005
更新日期:2019-09-01 00:00:00
abstract::During the development of locomotion circuits it is essential that motoneurons with distinct subtype identities select the correct trajectories and target muscles. In vertebrates, the generation of motoneurons and myelinating glia depends on Olig2, one of the five Olig family bHLH transcription factors. We investigate...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.06.027
更新日期:2012-09-15 00:00:00
abstract::Pancreatic development depends on the transcription factor Pax6, which controls islet cell differentiation and hormone production. To understand the regulation of Pax6 pancreatic expression, we have identified a minimal Pax6 pancreatic enhancer and show that it contains a composite binding site for Meis and Pbx homeop...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.06.030
更新日期:2006-12-15 00:00:00
abstract::GATA4, a transcription factor expressed in the proximal small intestine but not in the distal ileum, maintains proximal-distal distinctions by multiple processes involving gene repression, gene activation, and cell fate determination. Friend of GATA (FOG) is an evolutionarily conserved family of cofactors whose member...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.07.022
更新日期:2008-10-01 00:00:00
abstract::The adult fungiform taste papilla is a complex of specialized cell types residing in the stratified squamous tongue epithelium. This unique sensory organ includes taste buds, papilla epithelium and lateral walls that extend into underlying connective tissue to surround a core of lamina propria cells. Fungiform papilla...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.07.022
更新日期:2013-10-01 00:00:00