Abstract:
:Gas chromatography-flame ionization detection (GC-FID) was used to assess the chemical profile and quantification of cannabinoids to identify the differences, if existing, in the chemical constituents of in vitro propagated plants (IVP), conventionally grown plants (VP) and indoor grown mother plants (MP-Indoor) of a high THC yielding variety of Cannabis sativa L. during different developmental stages of growth. In general, THC content in all groups increased with plant age up to a highest level during the budding stage where the THC content reached a plateau before the onset of senescence. The pattern of changes observed in the concentration of other cannabinoids content with plants age has followed a similar trend in all groups of plants. Qualitatively, cannabinoids profiles obtained using GC-FID, in MP-indoor, VP and IVP plants were found to be similar to each other and to that of the field grown mother plant (MP field) of C. sativa. Minor differences observed in cannabinoids concentration within and among the groups were not found to be statistically significant. Our results confirm the clonal fidelity of IVP plants of C. sativa and suggest that the biochemical mechanism used in this study to produce the micropropagated plants does not affect the metabolic content and can be used for the mass propagation of true to type plants of this species for commercial pharmaceutical use.
journal_name
Planta Medjournal_title
Planta medicaauthors
Chandra S,Lata H,Mehmedic Z,Khan IA,ElSohly MAdoi
10.1055/s-0029-1240628subject
Has Abstractpub_date
2010-05-01 00:00:00pages
743-50issue
7eissn
0032-0943issn
1439-0221journal_volume
76pub_type
杂志文章相关文献
PLANTA MEDICA文献大全abstract::Silybum marianum Gaertn. (Milk thistle) has been used since ancient times for the relief of liver diseases characterized by intense oxidative stress such as inflammatory liver disease and cirrhosis. As oxidative stress by hyperglycemia is involved in micro- and macrovascular complications of type 2 diabetes, our aim w...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-0042-113135
更新日期:2017-01-01 00:00:00
abstract::The anti-inflammatory activity of alpha-viniferin, a trimer of resveratrol, has been demonstrated in an animal model of carrageenin-induced paw edema, and inhibitory effects of the compound on cyclooxygenase (COX) and inducible nitric oxide synthase (iNOS) have been investigated in order to understand the mode of the ...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2003-42787
更新日期:2003-08-01 00:00:00
abstract::Paeoniae Radix (the roots of Paeonia lactiflora Pallas) is a crude drug that is used in Asia and Europe to improve blood flow. We studied its vasodilator effect and mechanisms of action in vitro. The extract from Paeoniae Radix (PRE) relaxed prostaglandin F2a-precontracted aortic ring preparations of isolated rat aort...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-957934
更新日期:1996-10-01 00:00:00
abstract::Callus induction starting from the rhizome tissue of plants of three CURCUMA species: CURCUMA ZEDOARIA Roscoe, C. DOMESTICA Valeton, and C. AROMATICA Salisbury (Zingiberaceae) was performed. Successful callus formation was obtained in all three species using Murashige-Skoog's (MS) medium supplemented with 1-naphthalen...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-962344
更新日期:1988-02-01 00:00:00
abstract::Juniperus (Cupressaceae, Pinales) plants are widely distributed in the Qinghai-Tibet Plateau of China. The leaves and twigs of at least 8 Juniperus species (J. pingii, J. pingii var. wilsonii, J. squamata, J. recurva var. coxii, J. saltuaria, J. indica, J. tibetica and J. convallium var. microsperma) have been used as...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/a-1194-0471
更新日期:2020-11-01 00:00:00
abstract::Croton caudatus is an important species in Dai folk medicine. Some other Croton species, in particular, C. kongensis, C. cascarilloides, C. crassifolius, C. lachnocarpus and C. olivaceus are often marketed as C. caudatus, and thus, the therapeutic effects of C. caudatus are not achieved. The respective morphological f...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2007-967197
更新日期:2007-06-01 00:00:00
abstract::Halimium halimifolium (Hh) is a shrub used in Algerian folk medicine to treat gastrointestinal pain. An UHPLC-PDA-ESI/MSn method was developed to identify the metabolic profile of the traditionally used infusion (Hh-A) from the aerial parts. The structures of flavanols were confirmed by NMR analysis after the isolatio...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/a-0953-6007
更新日期:2019-08-01 00:00:00
abstract::The aerial parts of DODONAEA VISCOSA afforded a new diterpenoid, dodonic acid, the structure and stereochemistry of which have been established by chemical and spectroscopic means. ...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2007-969764
更新日期:1984-10-01 00:00:00
abstract::The chloroform extract of the stems of Encelia ventorum yielded a new benzopyranbenzofuran dimer. The structure was assigned from (1)H-NMR and MS data as 4[2-(5-acetyl-6-hydroxybenzofuranyl)]-2-[6(7-methoxy-2, 2-dimethylbenzo-1 -pyranyl)]-pentan-2-ol. ...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2007-969992
更新日期:1983-04-01 00:00:00
abstract::Transformed root cultures of ipecac (Cephaelis ipecacuanha A. Richard), one of the recalcitrant woody plant species for Agrobacterium-mediated transformation, were established by co-culturing of in vitro petiole segments with Agrobacterium rhizogenes ATCC 15 834. Southern blot analysis of the established roots reveale...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2003-45149
更新日期:2003-11-01 00:00:00
abstract::A new tetrahydrofuran lignan, named henricine ( 1), was isolated from the stems of SCHISANDRA HENRYI. High resolution COSY spectrum was used in the structural elucidation. ...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2007-969265
更新日期:1986-12-01 00:00:00
abstract::Urinary metabolites of scutellarin, the main effective constituent of breviscapine, a cerebrovascular and cardiovascular drug consisting of total flavonoids of Erigeron breviscapus, were investigated in rats. Two major metabolites were isolated from the urine of rats following oral administration of scutellarin and id...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2007-967128
更新日期:2007-04-01 00:00:00
abstract::A new minor diterpenoid alkaloid, caeruline, was isolated from DELPHINIUM CAERULEUM Jacq. ex Camb. Its structure was elucidated as 1 on the basis of spectral evidence. Along with this new compound, three known diterpenoid alkaloids were also isolated from this plant and identified as tatsiensine, delcosine, and lycoct...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-959611
更新日期:1993-02-01 00:00:00
abstract::The roots of HAPLOPAPPUS FREEMONTII afforded in addition to the known 8-oxo-beta-cyperone two further derivatives. The structures were elucidated by highfield (1)H-NMR spectroscopy. ...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2007-969205
更新日期:1986-10-01 00:00:00
abstract::Cannabis (Cannabis sativa L.) is an annual herbaceous plant that belongs to the family Cannabaceae. Trans-Δ9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD) are the two major phytocannabinoids accounting for over 40% of the cannabis plant extracts, depending on the variety. At the University of Mississippi, differ...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-0043-124088
更新日期:2018-03-01 00:00:00
abstract::We previously reported that total coumarins, the major active components of Urtica dentata Hand, exhibited substantial protection against the development of autoimmune diabetes. Diabetic nephropathy is one of the most serious complications of diabetes and is closely correlated with end-stage renal disease. We used the...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-0035-1557866
更新日期:2015-10-01 00:00:00
abstract::ECa 233, a standardized extract of Centella asiatica, has been found to exhibit various positive neurological effects and to have a good safety profile. The present study aimed to explore the disposition kinetics of ECa 233, containing madecassoside (53.1 %) and asiaticoside (32.3 %), in rats. The extract was intraven...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-0042-122344
更新日期:2017-05-01 00:00:00
abstract::Rutaecarpine is an alkaloid originally isolated from the unripe fruit of Evodia rutaecarpa. Recently, rutaecarpine has been characterized to have an anti-inflammatory activity through cyclooxygenase-2 inhibition. In the present studies, the effects of rutaecarpine on liver cytochrome P450 s (P450s) and P450 s involved...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2004-827207
更新日期:2004-08-01 00:00:00
abstract::Phytoestrogens are discussed as candidate substances to treat symptoms related to estrogen deficiency. In in vitro experiments, the naturally occurring flavonoid 6-(1,1-dimethylallyl)naringenin (6-DMAN) emerged as one of the most potent phytoestrogenic substances. 6-DMAN is not as well characterized as other flavonoid...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2004-827178
更新日期:2004-07-01 00:00:00
abstract::The methanol extract of the defatted roots of Asparagus adscendens Roxb. yielded beta-sitosterol beta-D-glucoside, two new spirostanol glycosides (asparanin C and asparanin D) and two new furostanol glycosides (asparoside C and asparoside D). ...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2007-970018
更新日期:1982-09-01 00:00:00
abstract::We report the effects of a fraction (F1) obtained from Himanthalia elongata on spontaneous locomotor activity, hypermotility, motor coordination, rectal temperature, pentobarbitone-induced hypnosis, pentylenetetrazole-induced convulsions and analgesic activity in the writhing and hot-plate tests. This fraction caused ...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-959655
更新日期:1993-06-01 00:00:00
abstract::Ischemic preconditioning, which is mediated by cell signaling molecules, protects the heart from ischemia-reperfusion injury by limiting the infarct size. Oleuropein, the main polyphenolic constituent of olives, reduced the infarct size in normal and cholesterol-fed rabbits when it was administered at a nutritional do...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-0034-1383306
更新日期:2015-06-01 00:00:00
abstract::(3R,6R)-4-methyl-6-(1-methylethyl)-3-phenylmethylperhydro-1,4-oxazine-2,5-dione (1) was isolated from the fruiting bodies of Isaria japonica as an apoptosis-inducing agent. The complete structural assignment of the compound was accomplished on the basis of spectroscopic methods and chemical transformations. Compound 1...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2002-26744
更新日期:2002-04-01 00:00:00
abstract::From an extract of the whole plant of Ligularia virgaurea ssp. oligocephala, three new eremophilane sesquiterpene lactones, 10alpha-hydroxy-1-oxoeremophila-7(11),8(9)-dien-12,8-olide (1), 6alpha,10alpha-dihydroxy-1-oxoeremophila-7(11),8(9)-dien-12,8-olide (2), 6beta,10beta-dihydroxyeremophila-7(11),8(9)-dien-12,8-olid...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2004-818983
更新日期:2004-05-01 00:00:00
abstract::The known alkaloids (+/-)-stylopine, (+/-)-sinactine, protopine, (+)-bulbocapnine, (-)-domesticine, (+)-corydine and (+)-isoboldine were found in the mixture of alkaloids from Corydalis marschalliana (Papaveraceae), growing in Bulgaria. The alkaloids dehydronantenine, (+)-nantenine and oxonantenine were isolated for t...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2007-971718
更新日期:1981-03-01 00:00:00
abstract::From the roots of Aconitum crassicaule, two new diterpenoid alkaloids, crassicaulisine and crassicaulidine, were isolated. The structure of crassicaulisine, C (24)H (39)O (7)N, has been elucidated as 3 on the basis of chemical and spectral data, while the structure of crassicaulidine, C (24)H (39)O (8)N, has been show...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2007-969945
更新日期:1983-01-01 00:00:00
abstract::Anti-sera against a complement-activating pectin (AR-2IIb), which was purified from the roots of Angelica acutiloba Kitagawa, were obtained by immunization of rabbits, and a polyclonal anti-AR-2IIb antibody of the IgG class was purified by affinity chromatography on AR-2IIb-immobilized Sepharose and Protein G-Sepharos...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-959524
更新日期:1994-10-01 00:00:00
abstract::Antroquinonol ( 1), an ubiquinone derivative, was isolated from the solid-state fermented mycelium of Antrodia camphorata (Polyporaceae, Aphyllophorales), a parasitic fungus indigenous to Taiwan. The structure of compound 1 was elucidated by the analysis of their spectroscopic data. Its cytotoxic activities were evalu...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2007-990232
更新日期:2007-10-01 00:00:00
abstract::A quantitative analysis using (1)H-NMR (Q-NMR) has been developed for the determination of strychnine and brucine in Strychnos nux-vomica seeds and stems. The advantages of the method are that no reference alkaloids are needed for calibration curves, the quantification could be directly realized on a crude extract, st...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2003-818014
更新日期:2003-12-01 00:00:00
abstract::The essential oil composition of three Zingiberaceae widely used as medicinal aromatic plants from S. Tomé and Príncipe: Aframomum danielli (Hook. f.) K. Schum., Curcuma longa L. and Zingiber officinale Rosc. was studied. Two samples of the essential oils from fruit of A. danielli and from rhizomes of the other two sp...
journal_title:Planta medica
pub_type: 信件
doi:10.1055/s-2001-16494
更新日期:2001-08-01 00:00:00