Dual effect of glucose on cytoplasmic Ca2+ in single pancreatic beta-cells.

abstract：:Lysenin, a hemolytic protein derived from the body fluid of earthworm, was incorporated into artificial bilayer membranes. Upon insertion, it formed a voltage-dependent large conductance channel in asolectin bilayers in a sphingomyelin-dependent manner. The channel had low ion-selectivity. Single-channel conductance w...

journal_title：Biochemical and biophysical research communications

authors： Ide T,Aoki T,Takeuchi Y,Yanagida T

更新日期：2006-07-21 00:00:00

abstract：:Certain physiological events in anterior pituitary tissue are regulated by protein kinase C that is relatively resistant to the inhibitor, H7. In the present studies we have shown that two H7-resistant protein kinase C activities may be isolated from (pituitary) gonadotroph-derived alpha T3-1 cells using hydroxyapatit...

journal_title：Biochemical and biophysical research communications

authors： Simpson J,Johnson MS,Clegg RA,Mitchell R

更新日期：1996-01-26 00:00:00

abstract：:Metallothionein (MT) is effective in the prevention of diabetic cardiomyopathy, and hypoxia-inducible factor-1 (HIF-1) is known to control vascular endothelial growth factor (VEGF) gene expression and regulate angiogenesis in diabetic hearts. We examined whether or not MT affects HIF-1 activity in the heart of diabeti...

journal_title：Biochemical and biophysical research communications

authors： Feng W,Wang Y,Cai L,Kang YJ

更新日期：2007-08-17 00:00:00

abstract：:The present study demonstrates that human platelets possess a specific L-arginine transport system able to provide adequate amounts of L-arginine for endogenous nitric oxide production. L-arginine uptake takes place through a saturable high affinity carrier-mediated Na(+)-independent process which is significantly inh...

journal_title：Biochemical and biophysical research communications

authors： Vasta V,Meacci E,Farnararo M,Bruni P

更新日期：1995-01-26 00:00:00

abstract：:Hepcidin is a main regulator of iron metabolism, of which abnormal expression affects intestinal absorption and reticuloendothelial sequestration of iron by interacting with ferroportin. It is also noted that abnormal iron accumulation is one of the key factors to facilitate promotion and progression of cancer includi...

journal_title：Biochemical and biophysical research communications

authors： Toki Y,Sasaki K,Tanaka H,Yamamoto M,Hatayama M,Ito S,Ikuta K,Shindo M,Hasebe T,Nakajima S,Sawada K,Fujiya M,Torimoto Y,Ohtake T,Kohgo Y

更新日期：2016-08-05 00:00:00

abstract：:In this study terminal cisternae vesicles from rabbit skeletal muscle were fused into planar bilayers and the effect of calmodulin on single Ca2+ release channel currents was investigated. In the presence of 10(-7) and 10(-9) M free [Ca2+], nanomolar concentrations of calmodulin activated the channel by increasing the...

journal_title：Biochemical and biophysical research communications

authors： Buratti R,Prestipino G,Menegazzi P,Treves S,Zorzato F

更新日期：1995-08-24 00:00:00

abstract：:Treatment of the rat C6 glioma cell line with interleukin-1 beta (IL-1 beta) resulted in an accumulation of cytosolic phospholipase A2 (cPLA2) in mRNA level. The increase of cPLA2 in mRNA level was observed at 6 hours after treatment of IL-1 beta and reached to the maximal level at 12 hours. The accumulation also rema...

journal_title：Biochemical and biophysical research communications

authors： Ozaki M,Morii H,Qvist R,Watanabe Y

更新日期：1994-11-30 00:00:00

abstract：:The conversion of the endogenous cellular prion protein to an abnormally folded isoform is a hallmark of transmissible spongiform encephalopathies. It occurs when a misfolded prion protein contacts the cellular PrP. Among the molecular partners suggested to be involved in the misfolding process, the glycosaminoglycans...

journal_title：Biochemical and biophysical research communications

authors： Martin R,Chantepie S,Chapuis J,Le-Duc A,Maftah A,Papy-Garcia D,Laude H,Petit JM,Gallet PF

更新日期：2011-10-28 00:00:00

abstract：:Pyrrolidine dithiocarbamate (PDTC) is a synthetic antioxidant molecule, which has been recently proposed as an antitumoral agent on the basis of its capability of inducing apoptosis. We investigated the effect of PDTC on the proliferation and survival of the promyelocitic cell line HL-60. Concentration as low as 10 mi...

journal_title：Biochemical and biophysical research communications

authors： Della Ragione F,Cucciolla V,Borriello A,Della Pietra V,Manna C,Galletti P,Zappia V

更新日期：2000-02-24 00:00:00

abstract：:Unveiling the mechanism of the relapse of acute lymphoblastic leukemia (ALL) is the key to improve the prognosis of ALL and remains a huge challenge. Glycan-based interactions play a vital role in immune surveillance, cell-cell adhesion and cell-matrix interaction, contributing to treatment failure in tumor. However, ...

journal_title：Biochemical and biophysical research communications

authors： Yi L,Hu Q,Zhou J,Liu Z,Li H

更新日期：2019-02-26 00:00:00

abstract：:Small interfering RNAs (siRNAs) have become the most powerful and widely used gene silencing reagents for reverse functional genomics and molecular therapeutics. The key challenge for achieving effective gene silencing in particular for the purpose of the therapeutics is primarily dependent on the effectiveness and sp...

journal_title：Biochemical and biophysical research communications

authors： Hung CF,Lu KC,Cheng TL,Wu RH,Huang LY,Teng CF,Chang WT

更新日期：2006-08-04 00:00:00

abstract：:Peripheral blood monocytes (PBMC) promote vascular inflammation and atherosclerosis. Chlamydia pneumoniae (Cp) infection of PBMC is found in atherosclerotic patients, appears refractory to antibiotics, and may predispose to vascular damage. In Cp-infected human PBMC we analyzed the role of cyclooxygenase-2 (Cox-2) for...

journal_title：Biochemical and biophysical research communications

authors： Rupp J,Berger M,Reiling N,Gieffers J,Lindschau C,Haller H,Dalhoff K,Maass M

更新日期：2004-07-30 00:00:00

abstract：OBJECTIVE:Screening and identifying the gene mutation of EXT1, EXT2 and EXT3 associated with multiple exostosis (ME) and the expression in tumor tissues. METHODS:Nine patients with multiple exostosis were collected and genomic DNA was extracted. Polymerase chain reaction (PCR) amplification and direct sequencing techn...

journal_title：Biochemical and biophysical research communications

authors： Wu ZY,Wang Y,Wang JW,Chen YZ,Guo Y

更新日期：2018-11-10 00:00:00

abstract：:Akt/PKB plays a pivotal role in cell survival and proliferation. Previously, we reported that UV-irradiation induces extensive cell death in Akt2(-/-) mouse embryonic fibroblasts (MEFs) while Akt1(-/-) MEFs show cell cycle arrest. Here, we find that Akt1(-/-) MEFs exhibit phenotypic changes characteristics of senescen...

journal_title：Biochemical and biophysical research communications

authors： Jee HJ,Kim HJ,Kim AJ,Bae YS,Bae SS,Yun J

更新日期：2009-06-05 00:00:00

abstract：:We have constructed a hybrid multicomponent dioxygenase gene cluster in which the bphA1 gene, coding for ISP alpha subunit of biphenyl dioxygenase from Pseudomonas pseudoalcaligenes KF707, has been replaced by the bedC1 gene encoding the corresponding subunit of benzene dioxygenase from P. putida ML2. Escherichia coli...

journal_title：Biochemical and biophysical research communications

authors： Tan HM,Cheong CM

更新日期：1994-10-28 00:00:00

abstract：:Conventional ESR spectra of 16-doxyl-stearic acid bound to bovine and human serum albumin were recorded at different temperatures in order to investigate the status of spin-labeled fatty acid in the interior of the protein globule. A computer spectrum simulation of measured spectra, performed by non-linear least-squar...

journal_title：Biochemical and biophysical research communications

authors： Gurachevsky A,Shimanovitch E,Gurachevskaya T,Muravsky V

更新日期：2007-09-07 00:00:00

abstract：:The DNA segment harboring the promoter region and the exon 1 of the human tyrosinase gene has been cloned and characterized. Sequence analysis reveals the amino-terminal half of tyrosinase molecule including a signal peptide, of which six amino acid residues are not represented in the tyrosinase cDNA, pHT gamma 1 [Shi...

journal_title：Biochemical and biophysical research communications

authors： Takeda A,Tomita Y,Okinaga S,Tagami H,Shibahara S

更新日期：1989-08-15 00:00:00

abstract：:The genome of Saccharomyces cerevisia encodes four mismatch repair MutL proteins and these proteins form three heterocomplexes: Mlh1-Mlh2, Mlh1-Mlh3, and Mlh1-Pms1. Only, the Mlh1-Mlh3 heterocomplex has been implicated specifically in promotion of meiotic crossing-over. In this report, we show that yeast Mlh3 co-immun...

journal_title：Biochemical and biophysical research communications

authors： Wang TF,Kung WM

更新日期：2002-08-30 00:00:00

abstract：:Resonance energy transfer (RET) is widely used to detect proximity between biomolecules. In transparent solution the maximum donor-to-acceptor distance for RET is about 70 A. We measured the effects of metallic silver island films on RET from the intrinsic tryptophan of a protein to a bound probe as the acceptor. Thes...

journal_title：Biochemical and biophysical research communications

authors： Malicka J,Gryczynski I,Kusba J,Shen Y,Lakowicz JR

更新日期：2002-06-21 00:00:00

abstract：:Parkinson's disease (PD) is one of the most prevalent neurodegenerative diseases but its etiology is unclear. Alpha-synuclein (alpha-SN) is a major component of Lewy bodies and Lewy neurites, and its missense mutations, A30P and A53T, cause familial PD. In PD, alpha-SN-positive glial inclusions are distributed mainly ...

journal_title：Biochemical and biophysical research communications

authors： Kohno R,Sawada H,Kawamoto Y,Uemura K,Shibasaki H,Shimohama S

更新日期：2004-05-21 00:00:00

abstract：:The NUCKS gene is located on human chromosome 1q32.1 and consists of seven exons and six introns. The gene lacks a TATA box but contains two Inr elements, two GC boxes, and one consensus-binding site for E2F-1. NUCKS is expressed in all human adult and foetal tissues investigated, and has all the features of being a h...

journal_title：Biochemical and biophysical research communications

authors： Grundt K,Haga IV,Aleporou-Marinou V,Drosos Y,Wanvik B,Østvold AC

更新日期：2004-10-22 00:00:00

abstract：:Myeloperoxidase (MPO) is a glycosylated heme-containing enzyme present in the azurophilic granules of normal human polymorphonuclear neutrophils. This enzyme plays a major role in the microbicidal activity of the host defense system by catalyzing the formation of the potent oxidant, hypochlorous acid. Although the ami...

journal_title：Biochemical and biophysical research communications

authors： Taylor KL,Uhlinger DJ,Kinkade JM Jr

更新日期：1992-09-30 00:00:00

abstract：:In regions of adult neurogenesis, neural progenitor cells (NPCs) are found in close proximity to blood vessels within a so-called 'vascular niche'. Neurogenesis is linked to angiogenesis via certain growth factors. We propose that angiopoietin-1 (Ang1), which is similar to VEGF, has a unique role in neurogenesis indep...

journal_title：Biochemical and biophysical research communications

authors： Bai Y,Cui M,Meng Z,Shen L,He Q,Zhang X,Chen F,Xiao J

更新日期：2009-01-09 00:00:00

abstract：:The ATPase activity of F1 isolated from the thermophilic bacterium PS3 is stimulated at 30 degrees C by the anionic detergents cholate or deoxycholate. Maximal activity obtained with these detergents (35 mumol/min X mg) is similar to the activity reported for the optimal temperature, 75 degrees C. The activity is line...

journal_title：Biochemical and biophysical research communications

authors： Norling B

更新日期：1986-05-14 00:00:00

abstract：:Mannoproteins (MPs) are a major component of yeast cell walls and consist of high levels of mannose in covalent complexes with proteins. MPs complexly enhance the immune system. We previously isolated a mutant yeast, K48L3, with a higher yield of MP from its cell wall than wild-type Saccharomyces cerevisiae, YPH499. W...

journal_title：Biochemical and biophysical research communications

authors： Yoon BH,Lee SM,Chang HI,Ha CH

更新日期：2019-11-19 00:00:00

abstract：:The mechanism by which lithium (Li(+)) inhibits the protein kinase glycogen synthase kinase-3 (GSK-3) is unknown. Here, we demonstrate that Li(+) is a competitive inhibitor of GSK-3 with respect to magnesium (Mg(2+)), but not to substrate or ATP. This mode of inhibition is conserved between mammalian and Dictyostelium...

journal_title：Biochemical and biophysical research communications

authors： Ryves WJ,Harwood AJ

更新日期：2001-01-26 00:00:00

abstract：BACKGROUND:In diabetic retinopathy, prolonged high-level blood glucose induced significant impairments among various retinal tissues, including retinal pigment epithelial (RPE) cells. In an in vitro model of human RPE cells, we evaluated whether 7,8-Dihydroxyflavone (DHF) may effectively prevent high glucose-induced di...

journal_title：Biochemical and biophysical research communications

authors： Yu X,Liu Q,Wang X,Liu H,Wang Y

更新日期：2018-01-01 00:00:00

abstract：:Changes in phospholipid and fatty acid profile are hallmarks of cancer progression. Increase in peripheral benzodiazepine receptor expression has been implicated in breast cancer. The benzodiazepine, Ro5-4864, increases cell proliferation in some breast cancer cell lines. Biosynthesis of phosphatidylcholine (PC) has b...

journal_title：Biochemical and biophysical research communications

authors： Akech J,Sinha Roy S,Das SK

更新日期：2005-07-22 00:00:00

abstract：:The peptide deformylase in bacteria is involved in removal of N-formyl group from newly synthesized proteins. The gene encoding this enzyme from Mycobacterium tuberculosis was cloned and expressed in Escherichia coli. The enzyme activity of the recombinant protein (mPDF) was insensitive to modulation by common monoval...

journal_title：Biochemical and biophysical research communications

authors： Saxena R,Chakraborti PK

更新日期：2005-07-01 00:00:00

abstract：:In T lymphocyte, activation of Kv1.3 channel, the major voltage-dependent K(+) channel, is an essential step for cell proliferation in immune responses. Here, effects of anti-CD3 and anti-CD28 antibodies on Kv1.3 current were examined in three types of human T lymphocyte derived cell lines, Jurkat E6-1, p56lck-kinase ...

journal_title：Biochemical and biophysical research communications

authors： Matsushita Y,Ohya S,Itoda H,Kimura T,Suzuki Y,Yamamura H,Imaizumi Y

更新日期：2008-09-12 00:00:00