Abstract：
:The ability to predict endothelial cell migration rates may aid in the design of biomaterials that endothelialize following implantation. However, the complexity of the signaling response to migration-promoting stimuli such as sphingosine 1-phosphate (S1P) makes such predictions quite challenging. A number of signaling pathways impact S1P-mediated cell migration, including the Akt and Src pathways, which both affect activation of the small GTPase Rac. Rac activation promotes the formation of lamellipodia, and thus should be intimately linked to cell migration rates. In immortalized endothelial cells, expression of proteins that inhibit Akt, Src, and Rac (PTEN, CSK, and beta2-chimaerin, respectively) was decreased using RNA interference, resulting in increases in the basal level of activation of Akt, Src, and Rac. Cells were scrape-wounded and stimulated with 1 microM S1P. The timecourse of Akt, Src, and Rac activation was followed over 2 h in the perturbed cells, while migration into the scrape wound was measured over 6 h. Rac activation at 120 min post-stimulation was highly correlated with the mean migration rate of cells, but only in cells stimulated with S1P. Using partial least squares regression, the migration rate of cells into the scrape wound was found to be highly correlated with the magnitude of the early Akt peak (e.g., 5-15 min post-stimulation). These results demonstrated that biochemical measurements might be useful in predicting rates of endothelial cell migration.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Alford SK,Wang Y,Feng Y,Longmore GD,Elbert DLdoi
10.1007/s10439-010-0014-6subject
Has Abstractpub_date
2010-08-01 00:00:00pages
2775-90issue
8eissn
0090-6964issn
1573-9686journal_volume
38pub_type
杂志文章abstract：:A comparison was made between turbulence calculated by subtracting an ensemble average from the instantaneous velocity and calculations made with a high pass digital filter. Velocity was measured with a laser Doppler anemometer in vitro in the region of a normal porcine aortic valve and in patients with a hot film ane...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02584215
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abstract：:It would often be desirable to obtain the respiratory rate during everyday conditions without obtaining an additional respiratory trace. This study investigates the agreement between respiratory rate assessed from the electrocardiogram (ECG) and the reference respiratory rate derived from a nasal/oral airflow (AF). Na...
journal_title：Annals of biomedical engineering
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doi：10.1007/s10439-008-9580-2
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abstract：:Traumatic falls are a prevalent and costly threat to elderly adults. Accurate risk assessment is necessary for reducing incidence of falls. The objective of this study was to test the feasibility of a balance impairment detection model using tasks of sample categorization and falls risk estimation. Model design includ...
journal_title：Annals of biomedical engineering
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doi：10.1007/s10439-005-2867-7
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abstract：:Continuum-level finite element models (FEMs) of the humerus offer the ability to evaluate joint replacement designs preclinically; however, experimental validation of these models is critical to ensure accuracy. The objective of the current study was to quantify experimental full-field strain magnitudes within osteoar...
journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-014-1237-8
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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doi：
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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doi：10.1007/s10439-008-9494-z
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journal_title：Annals of biomedical engineering
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doi：10.1007/s10439-013-0915-2
更新日期：2014-03-01 00:00:00
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journal_title：Annals of biomedical engineering
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doi：10.1007/BF02410288
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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更新日期：1997-01-01 00:00:00
