Abstract：
:Human stem cells hold significant potential for the treatment of various diseases. However, their use as a therapy is hampered because of limited understanding of the mechanisms by which they respond to environmental stimuli. Efforts to understand extracellular biophysical cues have demonstrated the critical roles of geometrical and mechanical signals in determining the fate of stem cells. The goal of this study was to explore the interplay between cell polarity and matrix stiffness in stem cell lineage specification. We hypothesize that confining cells to asymmetric extracellular matrix islands will impart polarity at a single-cell level and will interact with mechanical signals to define the lineage of stem cells. To test these hypotheses, we employed microcontact printing to create patterned symmetric and asymmetric hydrogel islands of soft and hard surface stiffness. Human mesenchymal stem cells (hMSCs) were confined to these islands at the single-cell level and given the ability to differentiate along adipogenic or osteogenic routes. Our results demonstrated that cell polarity defines the lineage specification of hMSCs only on islands with low stiffness. Insight gained from this study provides a rational basis for designing stem cell cultures to enhance tissue engineering and regenerative medicine strategies.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Piroli ME,Jabbarzadeh Edoi
10.1007/s10439-018-2008-8subject
Has Abstractpub_date
2018-06-01 00:00:00pages
888-898issue
6eissn
0090-6964issn
1573-9686pii
10.1007/s10439-018-2008-8journal_volume
46pub_type
杂志文章abstract：:We propose a multifrequency energy transmission scheme for annular phased arrays in deep-body hyperthermia. The problem of frequency dependent resonance effects reported in hyperthermia phantom calibration studies, leading to skewed energy deposition patterns and unwanted energy maxima (hot spots), is addressed by com...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1430749
更新日期：2002-01-01 00:00:00
abstract：:Local hemodynamics are an important factor in atherosclerosis, from the development of early lesions, to the assessment of stroke risk, to determining the ultimate fate of a mature plaque. Until recently, our understanding of arterial fluid dynamics and their relationship to atherosclerosis was limited by the use of i...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章,评审
doi：10.1114/1.1467679
更新日期：2002-04-01 00:00:00
abstract：:Tissue engineering strategies have utilized a wide spectrum of synthetic and naturally-derived scaffold materials. Synthetic scaffolds are better defined and offer the ability to precisely and reproducibly control their properties, while naturally-derived scaffolds typically have inherent biological and structural pro...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章,评审
doi：10.1007/s10439-014-1192-4
更新日期：2015-03-01 00:00:00
abstract：:Interstitial fluid flow, critical for macromolecular transport, was recently shown to drive fibroblast differentiation and perpendicular cell and matrix alignment in 3D collagen cultures. Here we explore the mechanisms underlying this flow-induced cell and collagen alignment. Cell and matrix alignment was assessed fro...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-005-9067-3
更新日期：2006-03-01 00:00:00
abstract：:Mechanical properties of the cardiac tissue play an important role in normal heart function. The goal of this study was to determine the passive mechanical properties of all heart chambers through a paired comparison study in an ovine model. Ovine heart was used due its physiological and anatomical similarities to hum...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-016-1658-7
更新日期：2016-11-01 00:00:00
abstract：:It would often be desirable to obtain the respiratory rate during everyday conditions without obtaining an additional respiratory trace. This study investigates the agreement between respiratory rate assessed from the electrocardiogram (ECG) and the reference respiratory rate derived from a nasal/oral airflow (AF). Na...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-008-9580-2
更新日期：2008-12-01 00:00:00
abstract：:Newborns with single ventricle physiology are usually palliated with a multi-staged procedure. When cardiovascular complications e.g., collateral vessel formation occur during the inter-stage periods, further treatments are required. An 8-month-old patient, who underwent second stage (i.e., bi-directional Glenn, BDG) ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-014-1113-6
更新日期：2015-06-01 00:00:00
abstract：:Biodegradable implants have demonstrated clinical success in simple applications (e.g., absorbable sutures) and have shown great potential in many other areas of interventional medicine, such as localized drug delivery, engineered tissue scaffolding, and structural implants. For endovascular stenting and musculoskelet...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-015-1477-2
更新日期：2016-02-01 00:00:00
abstract：:In this study, we introduce the fast wavelet transform as a method for characterizing maturational changes in electrocortical activity, respiratory activity, and blood pressure in fetal lambs in early (110-122 days), mid (123-135 days), and late (136-144 days) third trimester (term 145 days). Each recording was 2 hr i...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02584457
更新日期：1995-09-01 00:00:00
abstract：:Microstructural characterization of cardiac tissue and its remodeling in disease is a crucial step in many basic research projects. We present a comprehensive approach for three-dimensional characterization of cardiac tissue at the submicrometer scale. We developed a compression-free mounting method as well as labelin...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-015-1465-6
更新日期：2016-05-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-020-02565-2
更新日期：2021-01-01 00:00:00
abstract：:We compare the influence of time-frequency methods on analysis of time-varying renal autoregulation properties. Particularly, we examine if detection probabilities are similar for amplitude and frequency modulation for a modulated simulation signal among five time-frequency approaches, and if time-varying changes in s...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-012-0625-1
更新日期：2013-01-01 00:00:00
abstract：:This study presents a novel approach to modeling the electrocardiogram (ECG): the Gaussian pulse decomposition. Constituent waves of the ECG are decomposed into and represented by Gaussian pulses using an iterative algorithm: the chip away decomposition (ChAD) algorithm. At each iteration, a nonlinear minimization met...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02648039
更新日期：1997-03-01 00:00:00
abstract：:Peripheral nerve injuries present challenges to regeneration. Currently, the gold standard for nerve repair is an autograft that results in another region of the body suffering nerve damage. Previously, bioactive borate glass (BBG) has been studied in clinical trials to treat patients with non-healing wounds, and we h...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-016-1689-0
更新日期：2016-12-01 00:00:00
abstract：:In this paper, an intelligent proportional-integral-derivative (PID) control method is introduced to the robotic testing system for the biomechanical study of human musculoskeletal joints. For the testing system, the robot is a highly nonlinear and heavily coupled complicated system, and the human spinal specimen also...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1023/b:abme.0000030759.80354.e8
更新日期：2004-06-01 00:00:00
abstract：:A computer-based feedback system has been developed to adjust the concentration of oxygen in the inspired gas of a patient under artificial respiration. The system uses a proportional plus integral controller and feedback of arterial oxygen saturation to adjust the inspired oxygen fraction. The effectiveness of the co...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02368173
更新日期：1992-01-01 00:00:00
abstract：:A wide range of length and time scales are relevant to pharmacology, especially in drug development, drug design and drug delivery. Therefore, multiscale computational modeling and simulation methods and paradigms that advance the linkage of phenomena occurring at these multiple scales have become increasingly importa...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章,评审
doi：10.1007/s10439-016-1563-0
更新日期：2016-09-01 00:00:00
abstract：:Obesity is a rising issue especially in the United States that can lead to heart problems, type II diabetes, and respiratory problems. Since the 1970s, obesity rates in the United States have more than doubled in adults and children. Recent evidence suggests that exposure to certain chemicals, termed "obesogens," in u...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-016-1752-x
更新日期：2017-07-01 00:00:00
abstract：:Functional electrical stimulation (FES) to lower extremity (LE) muscles is used by individuals post-stroke as an alternative to mechanical orthotic devices during gait or as a training modality during rehabilitation. Technological developments which improve the feasibility, accessibility and effectiveness of FES syste...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章,评审
doi：10.1007/s10439-014-1148-8
更新日期：2015-02-01 00:00:00
abstract：:The nose is the front line defender of the respiratory system. Unsteady simulations in three-dimensional models have been developed to study transport patterns in the human nose and its overall air-conditioning capacity. The results suggested that the healthy nose can efficiently provide about 90% of the heat and the ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-005-2513-4
更新日期：2005-04-01 00:00:00
abstract：:Icariin (Ic) has been demonstrated as a potent osteoinductive compound for bone tissue engineering. However, toxic side effects of the drug and poor biocompatibility of drug delivery systems (DDSs) still limit its application for bone repair in the clinic. To overcome these disadvantages and utilize the osteoinductivi...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-017-1900-y
更新日期：2017-11-01 00:00:00
abstract：:Experimental studies have shown that cardiac fibroblasts are electrically inexcitable, but can contribute to electrophysiology of myocardium in various manners. The aim of this computational study was to give insights in the electrophysiological role of fibroblasts and their interaction with myocytes. We developed a m...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-007-9405-8
更新日期：2008-01-01 00:00:00
abstract：:Cochlear implantation consists in electrically stimulating the auditory nerve by inserting an electrode array inside the cochlea, a bony structure of the inner ear. In the absence of any visual feedback, the insertion results in many cases of damages of the internal structures. This paper presents a feasibility study ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-020-02671-1
更新日期：2020-10-30 00:00:00
abstract：:The toxic or therapeutic effect of an inhaled aerosol is highly dependent upon the site and extent of deposition in the lung. A novel MRI-based method was used to quantify the spatial distribution of particles in the rat lung. Rats were exposed to 0.95 μm-diameter iron oxide particles in a controlled manner (N = 6) or...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-013-0745-2
更新日期：2013-05-01 00:00:00
abstract：:We propose a new method for guiding catheter ablation procedures to abolish sites of origin of arrhythmias. This method models both cardiac electrical activity and current pulses delivered from the tip of the ablation catheter with a single equivalent moving dipole (SEMD). The SEMD parameters are obtained from analysi...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1567281
更新日期：2003-05-01 00:00:00
abstract：:A rigorous analysis of blood flow must be based on the branching pattern and vascular geometry of the full vascular circuit of interest. It is experimentally difficult to reconstruct the entire vascular circuit of any organ because of the enormity of the vessels. The objective of the present study was to develop a nov...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-005-5758-z
更新日期：2005-08-01 00:00:00
abstract：BACKGROUND:Common-mode noise degrades cardiovascular signal quality and diminishes measurement accuracy. Filtering to remove noise components in the frequency domain often distorts the signal. METHOD:Two adaptive noise canceling (ANC) algorithms were tested to adjust weighted reference signals for optimal subtraction ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-007-9294-x
更新日期：2007-07-01 00:00:00
abstract：:Transient homotypic adhesions between flowing leukocytes and those previously adherent on the vessel wall has been proposed to amplify the accumulation of leukocytes at sites of inflammation. While adhesion of leukocytes to the vessel wall (primary capture) is mediated primarily by P-selectin on the endothelium and P-...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-008-9468-1
更新日期：2008-04-01 00:00:00
abstract：:The nonlinear dynamic properties of action potential encoding were studied in mechanosensory neurons innervating the slits of a slit-sense organ in the tropical wandering spider, Cupiennius salei. The organ contains two types of neurons that are morphologically similar but have different dynamic properties. Type A neu...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1355278
更新日期：2001-03-01 00:00:00
abstract：:In this paper, a novel cell stretcher design that mimics the real-time stretch of the heart wall is introduced. By culturing cells under stretched conditions that mimics the mechanical aspects of the native cardiac environment, better understanding on the role of biomechanical signaling on cell development can be achi...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-014-0976-x
更新日期：2014-05-01 00:00:00
