Granulocyte-colony-stimulatory factor: a strong inhibitor of natural killer cell function.

Abstract:

BACKGROUND:The human cytokine granulocyte-colony stimulatory factor (G-CSF) has found widespread application in the medical treatment of neutropenia and to mobilize hematopoietic stem cells used for transplantation. So far, the effect of G-CSF on natural killer (NK) cells has not been fully investigated. STUDY DESIGN AND METHODS:The effect of G-CSF on the phenotype, cytokine secretion profile, and cytotoxicity of NK cells was assessed. NK cells incubated in vitro in presence of G-CSF for 48 hours as well as NK cells isolated from peripheral blood of G-CSF-mobilized stem cell donors (in vivo) were used. RESULTS:In vitro, G-CSF caused a strongly altered phenotype in NK cells with 49% down regulation of NKp44 frequency. Furthermore, the expression of the activating receptors NKp46 and NKG2D decreased 40 and 64%, respectively. The expression of KIR2DL1 and KIR2DL2 decreased by 46% each. In cytotoxicity assays, the lytic capacity of G-CSF-exposed NK cells is reduced by up to 68% in vitro and up to 83% in vivo. Accordingly, granzyme B levels of in vivo G-CSF-exposed NK cells were reduced by up to 87% in comparison to nonstimulated NK cells. Cytokine production of in vitro and in vivo incubated NK cells was strongly decreased for interferon-γ, tumor necrosis factor-α, and granulocyte macrophage colony-stimulating factor as well as interleukin (IL)-6 and IL-8. Furthermore, we observed a reduction in proliferation and a positive feedback loop that increased the expression of the G-CSF receptor. CONCLUSION:G-CSF was demonstrated to be a strong inhibitor of NK cells activity and may prevent their graft-versus-leukemia effect after transplantation.

journal_name

Transfusion

journal_title

Transfusion

authors

Schlahsa L,Jaimes Y,Blasczyk R,Figueiredo C

doi

10.1111/j.1537-2995.2010.02820.x

subject

Has Abstract

pub_date

2011-02-01 00:00:00

pages

293-305

issue

2

eissn

0041-1132

issn

1537-2995

pii

TRF2820

journal_volume

51

pub_type

杂志文章
  • Further evaluation of a new standard of efficacy for stored platelets.

    abstract:BACKGROUND:The proposal to assess the viability capabilities of platelets (PLTs) collected, treated, or stored in a developmental system against "fresh" PLTs from the same subject poses several important methodologic issues pertaining to the timing and manner of the collecting and separating the fresh PLTs. This study ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2005.00177.x

    authors: AuBuchon JP,Herschel L,Roger J

    更新日期:2005-07-01 00:00:00

  • A randomized, double-blind comparison of donor tolerance of 400 mL, 200 mL, and sham red cell donation.

    abstract:BACKGROUND:Volume replacement could allow the safe collection of twice the normal amount of red cells in a standard donation. Studies in small numbers of donors have shown that a temporary decrease in red cell mass is well tolerated when donors give twice the usual amount (170-225 mL) of red cells in a standard 405- to...

    journal_title:Transfusion

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1046/j.1537-2995.1996.36896374369.x

    authors: Smith KJ,James DS,Hunt WC,McDonough W,Quintana R

    更新日期:1996-08-01 00:00:00

  • Hemostatic profile under fluid resuscitation during rivaroxaban anticoagulation: an in vitro survey.

    abstract:BACKGROUND:Uncontrollable bleeding is the leading cause of death in traumatically injured patients. The extent to which direct factor Xa inhibitors interfere with the applied resuscitation measures is presently unknown. STUDY DESIGN AND METHODS:In this study, we investigated the effect of the resuscitation fluids sali...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.14933

    authors: Helin TA,Zuurveld M,Manninen M,Meijers JCM,Lassila R,Brinkman HJM

    更新日期:2018-12-01 00:00:00

  • Validation of use of the Nageotte hemocytometer to count low levels of white cells in white cell-reduced platelet components.

    abstract:BACKGROUND:Determination of the white cell (WBC) count in WBC-reduced platelet components requires methods that have a detection limit in the range of approximately 5.0 x 10(2) to 5.0 x 10(4) per mL. STUDY DESIGN AND METHODS:With a 50-microL Nageotte hemocytometer and bright-field microscopy (200x magnification), stud...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1994.34194098600.x

    authors: Moroff G,Eich J,Dabay M

    更新日期:1994-01-01 00:00:00

  • Freeze-dried plasma stability under prehospital field conditions.

    abstract:BACKGROUND:This study evaluated the effect of routine, uncontrolled, Israeli field storage conditions on the stability and efficacy of Lyo-Plas N freeze-dried plasma (FDP). We evaluated clotting factors V, VIII, and XI; proteins S and C; fibrinogen; partial thromboplastin time (PTT); antithrombin III (ATIII); von Wille...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15533

    authors: Zur M,Glassberg E,Gorenbein P,Epstein E,Eisenkraft A,Misgav M,Avramovich E

    更新日期:2019-11-01 00:00:00

  • The symbolic roots of blood donation.

    abstract:BACKGROUND:Throughout history, blood has been embedded with powerful and paradoxical connotations. In the last decades, a more rational conception based on scientific knowledge has emerged, portraying blood as a "disenchanted" and utilitarian object, hence more apt for donation between strangers. This study aims to exa...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12477

    authors: Charbonneau J,Tran NY

    更新日期:2013-12-01 00:00:00

  • Human T-lymphotropic virus and transfusion safety: does one size fit all?

    abstract::Human T-cell leukemia viruses (HTLV-1 and HTLV-2) are associated with a variety of human diseases, including some severe ones. Transfusion transmission of HTLV through cellular blood components is undeniable. HTLV screening of blood donations became mandatory in different countries to improve the safety of blood suppl...

    journal_title:Transfusion

    pub_type: 杂志文章,评审

    doi:10.1111/trf.13329

    authors: Marano G,Vaglio S,Pupella S,Facco G,Catalano L,Piccinini V,Liumbruno GM,Grazzini G

    更新日期:2016-01-01 00:00:00

  • Lung transplantation complicated by graft-versus-host disease and confounded by incidental transfusion-associated macrochimerism.

    abstract:BACKGROUND:Passenger lymphocyte-mediated graft-versus-host disease (GVHD) in solid organ transplantation (SOT-GVHD) is considered a rare complication, particularly among recipients of lung allografts. The risk of transfusion-associated GVHD (TA-GVHD) in solid organ transplants is also considered rare. The suspicion of ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2008.01835.x

    authors: Pavenski K,Kamel-Reid S,Wei C,Cserti-Gazdewich CM

    更新日期:2008-10-01 00:00:00

  • The role of the paternal health history in cord blood banking.

    abstract:BACKGROUND:Umbilical cord blood (UCB) transplantation is becoming more widely used, yet ethical and policy issues regarding consent and health history persist. Whereas most UCB banks do not require paternal consent or paternal health history (PHH), both are obtained at this institution whenever possible. This study ass...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2002.00188.x

    authors: Askari S,Miller J,Clay M,Moran S,Chrysler G,McCullough J

    更新日期:2002-10-01 00:00:00

  • Maternal human platelet antigen-1a antibody level correlates with the platelet count in the newborns: a retrospective study.

    abstract:BACKGROUND:Maternal plasma and/or serum levels of anti-HPA-1a at delivery were compared to neonatal platelet (PLT) counts. STUDY DESIGN AND METHODS:Samples from HPA-1bb women who gave birth to children with thrombocytopenia or had anamnestic information about a previous child with neonatal alloimmune thrombocytopenia ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2007.01063.x

    authors: Killie MK,Husebekk A,Kaplan C,Taaning E,Skogen B

    更新日期:2007-01-01 00:00:00

  • Creating a uniform donor medical history questionnaire.

    abstract::Blood Centers of California, Inc. (BCC) is a consortium of 19 independent blood centers that collectively draw approximately 800,000 units of whole blood each year. Over the past several years, a committee of BCC, composed primarily of medical and nursing directors at member institutions, developed a Uniform Donor Med...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1991.31491213302.x

    authors: Kolins J,Silvergleid AJ

    更新日期:1991-05-01 00:00:00

  • Detection of dengue, chikungunya, and Zika RNA in blood donors from Southeast Asia.

    abstract:BACKGROUND:Chikungunya (CHIKV), dengue (DENV), and Zika (ZIKV) viruses are of concern due to the potential of transfusion transmission in blood, especially in regions such as Southeast Asia where the viruses are endemic. The recent availability of nucleic acid testing (NAT) to screen blood donations on an automated pla...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.16110

    authors: Stanley J,Chongkolwatana V,Duong PT,Kitpoka P,Stramer SL,Dung NTT,Grimm KE,Pojanasingchod A,Suksomboonvong P,Galel SA

    更新日期:2020-10-07 00:00:00

  • Molecular analysis of inactive and active RHD alleles in native Congolese cohorts.

    abstract:BACKGROUND:In Africa, RHD alleles have not been fully characterized. The purpose of this study was to identify inactive and active RHD alleles at the molecular level in Congolese cohorts. STUDY DESIGN AND METHODS:Blood samples were collected from people living in central Congo populated by Teke ethnic group. A total o...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2009.02161.x

    authors: Touinssi M,Chapel-Fernandes S,Granier T,Bokilo A,Bailly P,Chiaroni J

    更新日期:2009-07-01 00:00:00

  • Donor body mass index is an important factor that affects peripheral blood progenitor cell yield in healthy donors after mobilization with granulocyte-colony-stimulating factor.

    abstract:BACKGROUND:The use of hematopoietic progenitor cell (HPC) transplantation has rapidly expanded in recent years. Currently, several sources of HPCs are available for transplantation including peripheral blood HPCs (PBPCs), cord blood cells, and marrow cells. Of these, PBPC collection has become the major source of HPCs....

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12238

    authors: Chen J,Burns KM,Babic A,Carrum G,Kennedy M,Segura FJ,Garcia S,Potts S,Leveque C

    更新日期:2014-01-01 00:00:00

  • Transfusion-transmitted HBV infection in an endemic area: the necessity of more sensitive screening for HBV carriers.

    abstract:BACKGROUND:By NAT, HBV DNA is occasionally detectable in blood donors with past HBV infection but negative for HBsAg. Whether or not these donors can cause transfusion-transmitted HBV infections is uncertain. STUDY DESIGN AND METHODS:To determine whether or not donors with past HBV infection but negative for HbsAg can...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2002.00274.x

    authors: Wang JT,Lee CZ,Chen PJ,Wang TH,Chen DS

    更新日期:2002-12-01 00:00:00

  • Rare detection of hepatitis B and hepatitis C virus genomes by polymerase chain reaction in seronegative donors with elevated alanine aminotransferase.

    abstract:BACKGROUND:Since screening for antibody to hepatitis C virus (HCV) was introduced in 1990, posttransfusion hepatitis has been reduced to nearly background levels. This has led to reconsideration of the value of testing donated blood for elevated alanine aminotransferase (ALT). The contribution of ALT testing in detecti...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1994.34894353458.x

    authors: Sankary TM,Yang G,Romeo JM,Ulrich PP,Busch MP,Rawal BD,Vyas GN

    更新日期:1994-08-01 00:00:00

  • Evaluation and improvement of blood donor educational materials: results from a multicenter randomized controlled trial.

    abstract:BACKGROUND:Blood collection centers are charged with creating donor educational materials (DnEM) that are easily understood across all prospective donor populations, while addressing mandates and recommendations from regulatory agencies and professional standard setting organizations. Donors must have sufficient inform...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15866

    authors: Wehrli G,Rossmann SN,Waxman DA,Katz LM

    更新日期:2020-08-01 00:00:00

  • Evaluation of the properties of components prepared and stored after holding of whole blood units for 8 and 24 hours at ambient temperature.

    abstract:BACKGROUND:The capability of holding whole blood (WB) units at ambient temperature, overnight, should help in platelet (PLT) concentrate preparation logistics. We summarize the results of a study conducted in the early 1990s that compared, in particular, PLT and red blood cell (RBC) in vivo viability properties followi...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2010.02958.x

    authors: Moroff G,AuBuchon JP,Pickard C,Whitley PH,Heaton WA,Holme S

    更新日期:2011-01-01 00:00:00

  • Storage time affects umbilical cord blood viability.

    abstract:BACKGROUND:Cryopreserved umbilical cord blood (CB) is increasingly used as a cell source to reconstitute marrow in hematopoietic stem cell transplant patients. Delays in cryopreservation may adversely affect cell viability, thereby reducing their potential for engraftment after transplantation. STUDY DESIGN AND METHOD...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12481

    authors: Guttridge MG,Soh TG,Belfield H,Sidders C,Watt SM

    更新日期:2014-05-01 00:00:00

  • The controversy of unmodified versus citrated blood transfusion in the early 20th century.

    abstract::Many strides were made in the field of blood transfusion before and during World War I. However, it was not until after the war that one of the most exciting and controversial episodes in the field of blood transfusion occurred. Review of the literature revealed the 1920s to be a tumultuous era filled with conflicting...

    journal_title:Transfusion

    pub_type: 历史文章,杂志文章

    doi:10.1046/j.1537-2995.1984.24585017829.x

    authors: Wain SL

    更新日期:1984-09-01 00:00:00

  • Red cell autoantibodies in patients with acquired immune deficiency syndrome.

    abstract::Mild-to-profound anemia, thrombocytopenia, and rarely neutropenia have been observed in patients with the acquired immune deficiency syndrome (AIDS). To investigate a possible immune mechanism, blood samples from 28 hospitalized AIDS patients, four asymptomatic homosexual men, four homosexual men with the AIDS-related...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1986.26587020112.x

    authors: McGinniss MH,Macher AM,Rook AH,Alter HJ

    更新日期:1986-09-01 00:00:00

  • Hypersensitivity transfusion reactions to platelet concentrate: a retrospective analysis of the French hemovigilance network.

    abstract:BACKGROUND:Among labile blood products, platelet concentrates (PCs) are the leading cause of hypersensitivity transfusion reactions (HTRs). These reactions often lead to interruption of PC transfusion and can result in a prolonged transfusion process leading to significant morbidity and use of premedication and close m...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15275

    authors: Mertes PM,Tacquard C,Andreu G,Kientz D,Gross S,Malard L,Drouet C,Carlier M,Gachet C,Sandid I,Boudjedir K

    更新日期:2020-03-01 00:00:00

  • Adoption trends of point of issue Verax PGD rapid test for bacterial screening of platelets between 2013 and 2018 among hospitals supplied by the American Red Cross and impact on platelet availability.

    abstract:BACKGROUND:Point-of-issue tests, such as the Verax Pan Genera Detection (PGD) test, can be used to mitigate the occurrence of septic reactions. Little is known about how widespread the adoption of the PGD test is across US hospitals. STUDY DESIGN/METHODS:The Red Cross hemovigilance database was used to determine the n...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15817

    authors: Rios J,Westra J,Dy B,Young PP

    更新日期:2020-07-01 00:00:00

  • Acquisition of cytomegalovirus infection in infants following exchange transfusion: a prospective study.

    abstract::A prospective study of newborn infants who required exchange transfusion was undertaken to evaluate the risk of transmission of cytomegalovirus (CMV). Buffy coat-, urine- and saliva-saturated throat swabs for viral cultures and serum specimens for CMV complement-fixing (CF) antibody were obtained from 45 infant-mother...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1980.20380214901.x

    authors: Kumar A,Nankervis GA,Cooper AR,Gold E,Kumar ML

    更新日期:1980-05-01 00:00:00

  • Effects of immediate or delayed addition of platelet additive solution on the in vitro quality of apheresis platelets.

    abstract:BACKGROUND:There is little knowledge how different hold times of hyperconcentrated platelet (PLT) suspensions (HPSs) before the addition of platelet additive solution (PAS) might affect PLT quality. We compared the in vitro quality of single-donor PLT concentrates (SDPs) with immediate or delayed PAS addition and studi...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2011.03435.x

    authors: Ringwald J,Tully S,Geier C,Hauck B,Weiss D,Callaert M,Eckstein R

    更新日期:2012-06-01 00:00:00

  • Serological complications of a major ABO incompatible bone marrow transplantation in a Polynesian with aplastic anemia.

    abstract::A 24-year-old, blood-group O Polynesian woman with multiple red blood cell antibodies received a group A, HLA-compatible, sex-matched bone marrow transplantation as treatment for severe aplastic anemia. High retransplantation titers of ABO isohemagglutinins were successfully reduced by repeated plasmaphereses. Despite...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1982.22182154221.x

    authors: Ockelford PA,Hill RS,Nelson L,Blacklock HA,Woodfield DG,Matthews JR

    更新日期:1982-01-01 00:00:00

  • Treatment of hyperkalemia with exchange transfusion.

    abstract::Exchange transfusions using whole blood less than 24 hours old and deglycerolized red cells reconstituted with fresh-frozen plasma were performed in two premature infants for the treatment of hyperkalemia and cardiac arrhythmia. Improvement of their condition was immediate. ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1985.25485273824.x

    authors: Lorch V,Jones FS,Hoersten IR

    更新日期:1985-07-01 00:00:00

  • Removal of Yersinia enterocolitica from AS-1 red cells.

    abstract::The growth of Yersinia enterocolitica in AS-1 red cells was investigated so as to study the organism's proliferation kinetics and to evaluate the effect of prestorage white cell (WBC) reduction on bacterial multiplication. Twenty-four 2-unit pools of ABO-compatible whole blood were prepared and inoculated with Y. ente...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1992.32792391043.x

    authors: Buchholz DH,AuBuchon JP,Snyder EL,Kandler R,Edberg S,Piscitelli V,Pickard C,Napychank P

    更新日期:1992-09-01 00:00:00

  • Factor VIII concentrate prepared from blood donors stimulated by intranasal administration of a vasopressin analogue.

    abstract::With an interval of eight weeks between collections, blood was drawn twice from 120 blood donors. At one of the donations, 0.25 ml of synthetic vasopressin, (DDAVP, 1300 micrograms/ml), was administered intranasally 60 minutes prior to collection of the blood. No drug was given at the other donation. The yield of fact...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1982.22382224947.x

    authors: Mikaelsson M,Nilsson IM,Vilhardt H,Wiechel B

    更新日期:1982-05-01 00:00:00

  • Incorporation of plasticizer into red cells during storage.

    abstract::The development of flexible plastic blood bags has permitted effective blood component production and therapy. However, the plasticizer di(2-ethylhexyl)phthalate (DEHP), whose toxicity in humans is still undefined, is known to leach from the plastic into stored blood. Despite the availability of bags made of plastics ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1984.24685066808.x

    authors: Rock G,Tocchi M,Ganz PR,Tackaberry ES

    更新日期:1984-11-01 00:00:00