Abstract:
BACKGROUND:The human cytokine granulocyte-colony stimulatory factor (G-CSF) has found widespread application in the medical treatment of neutropenia and to mobilize hematopoietic stem cells used for transplantation. So far, the effect of G-CSF on natural killer (NK) cells has not been fully investigated. STUDY DESIGN AND METHODS:The effect of G-CSF on the phenotype, cytokine secretion profile, and cytotoxicity of NK cells was assessed. NK cells incubated in vitro in presence of G-CSF for 48 hours as well as NK cells isolated from peripheral blood of G-CSF-mobilized stem cell donors (in vivo) were used. RESULTS:In vitro, G-CSF caused a strongly altered phenotype in NK cells with 49% down regulation of NKp44 frequency. Furthermore, the expression of the activating receptors NKp46 and NKG2D decreased 40 and 64%, respectively. The expression of KIR2DL1 and KIR2DL2 decreased by 46% each. In cytotoxicity assays, the lytic capacity of G-CSF-exposed NK cells is reduced by up to 68% in vitro and up to 83% in vivo. Accordingly, granzyme B levels of in vivo G-CSF-exposed NK cells were reduced by up to 87% in comparison to nonstimulated NK cells. Cytokine production of in vitro and in vivo incubated NK cells was strongly decreased for interferon-γ, tumor necrosis factor-α, and granulocyte macrophage colony-stimulating factor as well as interleukin (IL)-6 and IL-8. Furthermore, we observed a reduction in proliferation and a positive feedback loop that increased the expression of the G-CSF receptor. CONCLUSION:G-CSF was demonstrated to be a strong inhibitor of NK cells activity and may prevent their graft-versus-leukemia effect after transplantation.
journal_name
Transfusionjournal_title
Transfusionauthors
Schlahsa L,Jaimes Y,Blasczyk R,Figueiredo Cdoi
10.1111/j.1537-2995.2010.02820.xsubject
Has Abstractpub_date
2011-02-01 00:00:00pages
293-305issue
2eissn
0041-1132issn
1537-2995pii
TRF2820journal_volume
51pub_type
杂志文章相关文献
TRANSFUSION文献大全abstract:BACKGROUND:The proposal to assess the viability capabilities of platelets (PLTs) collected, treated, or stored in a developmental system against "fresh" PLTs from the same subject poses several important methodologic issues pertaining to the timing and manner of the collecting and separating the fresh PLTs. This study ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2005.00177.x
更新日期:2005-07-01 00:00:00
abstract:BACKGROUND:Volume replacement could allow the safe collection of twice the normal amount of red cells in a standard donation. Studies in small numbers of donors have shown that a temporary decrease in red cell mass is well tolerated when donors give twice the usual amount (170-225 mL) of red cells in a standard 405- to...
journal_title:Transfusion
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1537-2995.1996.36896374369.x
更新日期:1996-08-01 00:00:00
abstract:BACKGROUND:Uncontrollable bleeding is the leading cause of death in traumatically injured patients. The extent to which direct factor Xa inhibitors interfere with the applied resuscitation measures is presently unknown. STUDY DESIGN AND METHODS:In this study, we investigated the effect of the resuscitation fluids sali...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14933
更新日期:2018-12-01 00:00:00
abstract:BACKGROUND:Determination of the white cell (WBC) count in WBC-reduced platelet components requires methods that have a detection limit in the range of approximately 5.0 x 10(2) to 5.0 x 10(4) per mL. STUDY DESIGN AND METHODS:With a 50-microL Nageotte hemocytometer and bright-field microscopy (200x magnification), stud...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1994.34194098600.x
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:This study evaluated the effect of routine, uncontrolled, Israeli field storage conditions on the stability and efficacy of Lyo-Plas N freeze-dried plasma (FDP). We evaluated clotting factors V, VIII, and XI; proteins S and C; fibrinogen; partial thromboplastin time (PTT); antithrombin III (ATIII); von Wille...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15533
更新日期:2019-11-01 00:00:00
abstract:BACKGROUND:Throughout history, blood has been embedded with powerful and paradoxical connotations. In the last decades, a more rational conception based on scientific knowledge has emerged, portraying blood as a "disenchanted" and utilitarian object, hence more apt for donation between strangers. This study aims to exa...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12477
更新日期:2013-12-01 00:00:00
abstract::Human T-cell leukemia viruses (HTLV-1 and HTLV-2) are associated with a variety of human diseases, including some severe ones. Transfusion transmission of HTLV through cellular blood components is undeniable. HTLV screening of blood donations became mandatory in different countries to improve the safety of blood suppl...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/trf.13329
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Passenger lymphocyte-mediated graft-versus-host disease (GVHD) in solid organ transplantation (SOT-GVHD) is considered a rare complication, particularly among recipients of lung allografts. The risk of transfusion-associated GVHD (TA-GVHD) in solid organ transplants is also considered rare. The suspicion of ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2008.01835.x
更新日期:2008-10-01 00:00:00
abstract:BACKGROUND:Umbilical cord blood (UCB) transplantation is becoming more widely used, yet ethical and policy issues regarding consent and health history persist. Whereas most UCB banks do not require paternal consent or paternal health history (PHH), both are obtained at this institution whenever possible. This study ass...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2002.00188.x
更新日期:2002-10-01 00:00:00
abstract:BACKGROUND:Maternal plasma and/or serum levels of anti-HPA-1a at delivery were compared to neonatal platelet (PLT) counts. STUDY DESIGN AND METHODS:Samples from HPA-1bb women who gave birth to children with thrombocytopenia or had anamnestic information about a previous child with neonatal alloimmune thrombocytopenia ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2007.01063.x
更新日期:2007-01-01 00:00:00
abstract::Blood Centers of California, Inc. (BCC) is a consortium of 19 independent blood centers that collectively draw approximately 800,000 units of whole blood each year. Over the past several years, a committee of BCC, composed primarily of medical and nursing directors at member institutions, developed a Uniform Donor Med...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1991.31491213302.x
更新日期:1991-05-01 00:00:00
abstract:BACKGROUND:Chikungunya (CHIKV), dengue (DENV), and Zika (ZIKV) viruses are of concern due to the potential of transfusion transmission in blood, especially in regions such as Southeast Asia where the viruses are endemic. The recent availability of nucleic acid testing (NAT) to screen blood donations on an automated pla...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.16110
更新日期:2020-10-07 00:00:00
abstract:BACKGROUND:In Africa, RHD alleles have not been fully characterized. The purpose of this study was to identify inactive and active RHD alleles at the molecular level in Congolese cohorts. STUDY DESIGN AND METHODS:Blood samples were collected from people living in central Congo populated by Teke ethnic group. A total o...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2009.02161.x
更新日期:2009-07-01 00:00:00
abstract:BACKGROUND:The use of hematopoietic progenitor cell (HPC) transplantation has rapidly expanded in recent years. Currently, several sources of HPCs are available for transplantation including peripheral blood HPCs (PBPCs), cord blood cells, and marrow cells. Of these, PBPC collection has become the major source of HPCs....
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12238
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:By NAT, HBV DNA is occasionally detectable in blood donors with past HBV infection but negative for HBsAg. Whether or not these donors can cause transfusion-transmitted HBV infections is uncertain. STUDY DESIGN AND METHODS:To determine whether or not donors with past HBV infection but negative for HbsAg can...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2002.00274.x
更新日期:2002-12-01 00:00:00
abstract:BACKGROUND:Since screening for antibody to hepatitis C virus (HCV) was introduced in 1990, posttransfusion hepatitis has been reduced to nearly background levels. This has led to reconsideration of the value of testing donated blood for elevated alanine aminotransferase (ALT). The contribution of ALT testing in detecti...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1994.34894353458.x
更新日期:1994-08-01 00:00:00
abstract:BACKGROUND:Blood collection centers are charged with creating donor educational materials (DnEM) that are easily understood across all prospective donor populations, while addressing mandates and recommendations from regulatory agencies and professional standard setting organizations. Donors must have sufficient inform...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15866
更新日期:2020-08-01 00:00:00
abstract:BACKGROUND:The capability of holding whole blood (WB) units at ambient temperature, overnight, should help in platelet (PLT) concentrate preparation logistics. We summarize the results of a study conducted in the early 1990s that compared, in particular, PLT and red blood cell (RBC) in vivo viability properties followi...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.02958.x
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Cryopreserved umbilical cord blood (CB) is increasingly used as a cell source to reconstitute marrow in hematopoietic stem cell transplant patients. Delays in cryopreservation may adversely affect cell viability, thereby reducing their potential for engraftment after transplantation. STUDY DESIGN AND METHOD...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12481
更新日期:2014-05-01 00:00:00
abstract::Many strides were made in the field of blood transfusion before and during World War I. However, it was not until after the war that one of the most exciting and controversial episodes in the field of blood transfusion occurred. Review of the literature revealed the 1920s to be a tumultuous era filled with conflicting...
journal_title:Transfusion
pub_type: 历史文章,杂志文章
doi:10.1046/j.1537-2995.1984.24585017829.x
更新日期:1984-09-01 00:00:00
abstract::Mild-to-profound anemia, thrombocytopenia, and rarely neutropenia have been observed in patients with the acquired immune deficiency syndrome (AIDS). To investigate a possible immune mechanism, blood samples from 28 hospitalized AIDS patients, four asymptomatic homosexual men, four homosexual men with the AIDS-related...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1986.26587020112.x
更新日期:1986-09-01 00:00:00
abstract:BACKGROUND:Among labile blood products, platelet concentrates (PCs) are the leading cause of hypersensitivity transfusion reactions (HTRs). These reactions often lead to interruption of PC transfusion and can result in a prolonged transfusion process leading to significant morbidity and use of premedication and close m...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15275
更新日期:2020-03-01 00:00:00
abstract:BACKGROUND:Point-of-issue tests, such as the Verax Pan Genera Detection (PGD) test, can be used to mitigate the occurrence of septic reactions. Little is known about how widespread the adoption of the PGD test is across US hospitals. STUDY DESIGN/METHODS:The Red Cross hemovigilance database was used to determine the n...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15817
更新日期:2020-07-01 00:00:00
abstract::A prospective study of newborn infants who required exchange transfusion was undertaken to evaluate the risk of transmission of cytomegalovirus (CMV). Buffy coat-, urine- and saliva-saturated throat swabs for viral cultures and serum specimens for CMV complement-fixing (CF) antibody were obtained from 45 infant-mother...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1980.20380214901.x
更新日期:1980-05-01 00:00:00
abstract:BACKGROUND:There is little knowledge how different hold times of hyperconcentrated platelet (PLT) suspensions (HPSs) before the addition of platelet additive solution (PAS) might affect PLT quality. We compared the in vitro quality of single-donor PLT concentrates (SDPs) with immediate or delayed PAS addition and studi...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2011.03435.x
更新日期:2012-06-01 00:00:00
abstract::A 24-year-old, blood-group O Polynesian woman with multiple red blood cell antibodies received a group A, HLA-compatible, sex-matched bone marrow transplantation as treatment for severe aplastic anemia. High retransplantation titers of ABO isohemagglutinins were successfully reduced by repeated plasmaphereses. Despite...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1982.22182154221.x
更新日期:1982-01-01 00:00:00
abstract::Exchange transfusions using whole blood less than 24 hours old and deglycerolized red cells reconstituted with fresh-frozen plasma were performed in two premature infants for the treatment of hyperkalemia and cardiac arrhythmia. Improvement of their condition was immediate. ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1985.25485273824.x
更新日期:1985-07-01 00:00:00
abstract::The growth of Yersinia enterocolitica in AS-1 red cells was investigated so as to study the organism's proliferation kinetics and to evaluate the effect of prestorage white cell (WBC) reduction on bacterial multiplication. Twenty-four 2-unit pools of ABO-compatible whole blood were prepared and inoculated with Y. ente...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1992.32792391043.x
更新日期:1992-09-01 00:00:00
abstract::With an interval of eight weeks between collections, blood was drawn twice from 120 blood donors. At one of the donations, 0.25 ml of synthetic vasopressin, (DDAVP, 1300 micrograms/ml), was administered intranasally 60 minutes prior to collection of the blood. No drug was given at the other donation. The yield of fact...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1982.22382224947.x
更新日期:1982-05-01 00:00:00
abstract::The development of flexible plastic blood bags has permitted effective blood component production and therapy. However, the plasticizer di(2-ethylhexyl)phthalate (DEHP), whose toxicity in humans is still undefined, is known to leach from the plastic into stored blood. Despite the availability of bags made of plastics ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1984.24685066808.x
更新日期:1984-11-01 00:00:00