Abstract:
BACKGROUND:Since screening for antibody to hepatitis C virus (HCV) was introduced in 1990, posttransfusion hepatitis has been reduced to nearly background levels. This has led to reconsideration of the value of testing donated blood for elevated alanine aminotransferase (ALT). The contribution of ALT testing in detecting seronegative infection was evaluated by the performance of polymerase chain reaction (PCR) for hepatitis B virus (HBV) or HCV in plasma from ALT-elevated blood units. STUDY DESIGN AND METHODS:Testing was performed on 375 units of plasma, derived from an equivalent of 47,500 blood donations, with a highly sensitive hemi-nested PCR procedure. Using a triplet of primers directed at the conserved regions of HBV DNA and 5'-noncoding regions of HCV RNA, the hemi-nested PCR assay can reliably amplify 10 viral molecules to levels detectable in ethidium bromide-stained agarose gels. Pools of plasma from groups of four donors were screened with hemi-nested PCR. For any reactive pools, the plasma from individual donors was retested twice on different aliquots. RESULTS:Two of 375 units, both with midrange ALT elevation, were repeatedly reactive in hemi-nested PCR (one each for HBV DNA and HCV RNA). However, samples from the two suspect donors tested 9 and 5 months later revealed no seroconversion, elevated ALT, or viral genomes in hemi-nested PCR. CONCLUSION:The lack of confirmed HBV or HCV infection in this study representing an estimated 47,500 voluntary blood donations suggests that routine ALT testing for further prevention of posttransfusion hepatitis after exclusion of HBV- and/or HCV-seropositive blood may be superfluous.
journal_name
Transfusionjournal_title
Transfusionauthors
Sankary TM,Yang G,Romeo JM,Ulrich PP,Busch MP,Rawal BD,Vyas GNdoi
10.1046/j.1537-2995.1994.34894353458.xsubject
Has Abstractpub_date
1994-08-01 00:00:00pages
656-60issue
8eissn
0041-1132issn
1537-2995journal_volume
34pub_type
杂志文章相关文献
TRANSFUSION文献大全abstract:BACKGROUND:An optimal cooling rate is critical for cryopreserving cord blood stem cells, so it is generally achieved by using controlled-rate freezers (CRFs). The cooling rate can be interrupted by many factors during cooling; therefore, a proper response to the interruption in cryopreservation is crucial to prevent th...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12774
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:When bacteria are found, after a platelet transfusion, in the recipient's blood as well as in the platelet concentrate (PC), a causal relationship is normally suspected, with the PC as the causative agent. The other alternative, that the patient has bacteremia and contaminated the PC, is less well documented...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1995.351096026371.x
更新日期:1995-10-01 00:00:00
abstract:BACKGROUND:Human immunodeficiency virus 1 (HIV-1) subtype B is predominant in Spain. However, the recent arrival of immigrant populations has increased the prevalence of non-B subtypes and circulating recombinant forms. The objective of this study was to determine the prevalence of HIV-1 subtypes and transmitted drug-r...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14195
更新日期:2017-09-01 00:00:00
abstract::A 20-year-old man with chronic granulomatous disease (CGD) and who was receiving granulocyte transfusions for a refractory liver abscess was studied to compare the kinetics of 111In-labeled granulocytes with those of two functional granulocyte assays, nitroblue tetrazolium reduction and chemiluminescence. Transfused g...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1987.27187121467.x
更新日期:1987-01-01 00:00:00
abstract::The whole blood electrical conductivity method for predonation hematocrit determinations was studied. Forty capillary and venous blood samples were tested concurrently with electrical conductivity and centrifugation methods. The electrical conductivity method demonstrated acceptable accuracy but was less precise than ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1985.25385219908.x
更新日期:1985-05-01 00:00:00
abstract:INTRODUCTION:There is an increasing awareness to integrate patient blood management (PBM) within routine surgical care. Limited information about the implementation of PBM in colorectal cancer surgery is available. This is curious, as preoperative anemia, associated with increased morbidity, is highly prevalent in colo...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14807
更新日期:2018-10-01 00:00:00
abstract:BACKGROUND:There is evidence that physicians lack adequate transfusion medicine knowledge. To design needs-based educational interventions to address this gap, a validated assessment tool is required. Previously published exams have not been created or validated using rigorous psychometric methods. STUDY DESIGN AND ME...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12425
更新日期:2014-05-01 00:00:00
abstract:BACKGROUND:RBCs modified with cyanuric chloride activated methoxy-PEG (CmPEG; 5000 Da) are less immunogenic than untreated RBCs, and their use thus may reduce the risk of alloimmunization in chronically transfused patients. STUDY DESIGN AND METHODS:To further examine the potential utility of CmPEG-RBCs, the effects of...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2001.41101225.x
更新日期:2001-10-01 00:00:00
abstract::The development of flexible plastic blood bags has permitted effective blood component production and therapy. However, the plasticizer di(2-ethylhexyl)phthalate (DEHP), whose toxicity in humans is still undefined, is known to leach from the plastic into stored blood. Despite the availability of bags made of plastics ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1984.24685066808.x
更新日期:1984-11-01 00:00:00
abstract:BACKGROUND:Iron supplements reduce the risk of iron depletion in blood donors. Recent initiatives have sought to increase use of donor iron supplementation (IS), but a baseline description of blood donor supplement practices is needed. STUDY DESIGN AND METHODS:Presenting blood donors received a questionnaire inquiring...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15407
更新日期:2019-09-01 00:00:00
abstract:BACKGROUND:Although Creutzfeldt-Jakob disease (CJD) has been shown to be transmissible through blood components in rodent models, no human blood-to-blood transmission has been documented. If blood transmission were possible in humans, persons with hemophilia in the United States would be at higher risk of contracting C...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1998.38998409000.x
更新日期:1998-09-01 00:00:00
abstract::Ultraviolet B (UVB) irradiation of platelet concentrates (PCs) may prevent the development of posttransfusion HLA alloimmunization. As irradiation performed in a blood center or a hospital will probably be associated with a variable postirradiation delay before transfusion, the ability to store PCs after UVB irradiati...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1991.31691306243.x
更新日期:1991-07-01 00:00:00
abstract:BACKGROUND:Treating hemorrhage with blood transfusions in subjects previously hemodiluted with different colloidal plasma expanders, using fresh autologous blood or blood that has been stored for 2 weeks, allows identifying the interaction between type of plasma expander and differences in blood storage. STUDY DESIGN ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2012.03679.x
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:The anti-CD20 monoclonal antibody rituximab has immune-modulatory effects similar to intravenous immunoglobulin (IVIG). We performed a systematic review and meta-analysis to determine the efficacy and safety of rituximab in autoimmune diseases that are also treated with IVIG. STUDY DESIGN AND METHODS:The mo...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14841
更新日期:2018-11-01 00:00:00
abstract:BACKGROUND:Storage of pooled platelet concentrates (PCs) with yields above 3.0 x 10(11) platelets per unit in a 1-L PL-732 polyolefin container for 5 days often results in a drop in pH to below 6.0. Recently, new oxygen-permeable platelet containers (1-L PL-2410, 1-L and 1.5-L Compoflex) have been developed. The maxima...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1997.37597293876.x
更新日期:1997-05-01 00:00:00
abstract:BACKGROUND:Successful hematopoietic stem cell transplantation using stored umbilical cord blood (CB) largely depends on cell dose and quality of CB units. The aim of this study was to assess the degree of early apoptosis, in addition to cell viability and hematopoietic potential, in umbilical CB units after storage. S...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.13120
更新日期:2015-08-01 00:00:00
abstract:BACKGROUND:Hemolytic transfusion reactions are a rare, yet serious complication of red blood cell (RBC) transfusion. Patients with sickle cell disease (SCD) are at an increased risk for such reactions, because they are prone to make alloantibodies against transfused RBCs, complicating this integral part of their diseas...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15010
更新日期:2019-02-01 00:00:00
abstract:BACKGROUND:Red blood cell (RBC) alloimmunization is a concern for patients who receive multiple or chronic transfusions. Alloimmunization prevalence in transfused patients with bone marrow failure syndrome (BMFS) is unknown. This study aimed to determine physician practice for RBC antigen matching, immunization rates, ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.13608
更新日期:2016-06-01 00:00:00
abstract::Sixty English-language articles on the motivation and recruitment of blood donors and nondonors are reviewed and evaluated. Apparently researchers have been finding essentially the same results for approximately twenty years. Motivations to donate are: altruism/humanitarian, personal or family credit, social pressure,...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1977.17277151916.x
更新日期:1977-03-01 00:00:00
abstract::Intravenous immune globulin (IVIG) therapy has shown great success in a number of autoimmune and inflammatory conditions and its use continues to increase worldwide. There is growing awareness of significant side effects of high-dose IVIG: however, particularly severe hemolysis in patients that are non-group O. It has...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/trf.13097
更新日期:2015-07-01 00:00:00
abstract:BACKGROUND:Umbilical cord blood (UCB) can be used as hematopoietic stem cell source for transplantation. The success of a transplantation is highly correlated with the number of total nucleated cells (TNCs) and CD34+ cells in the UCB. Certain obstetric factors increase the yield of stem cells in the UCB. It is necessar...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2007.01617.x
更新日期:2008-05-01 00:00:00
abstract:BACKGROUND:Autologous umbilical cord blood (AutoUCB) has historically been cryopreserved for potential use in hematopoietic transplantation. Increasingly, private AutoUCB banking is performed for therapies unavailable today. A Phase I trial using AutoUCB treatment for early pediatric Type 1 diabetes afforded us an oppo...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2011.03556.x
更新日期:2012-10-01 00:00:00
abstract::Alanine aminotransferase (ALT) phenotype and serum activities were determined in 200 random volunteer blood donors. Serum enzyme activities were not significantly affected by the ALT phenotype (p greater than 0.1). Studies on 500 random volunteer blood donors showed significant differences in serum ALT levels between ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1982.22482251212.x
更新日期:1982-07-01 00:00:00
abstract:BACKGROUND:Red blood cell (RBC) alloantibodies to nonself antigens may develop after transfusion or pregnancy, leading to morbidity and mortality in the form of hemolytic transfusion reactions or hemolytic disease of the newborn. A better understanding of the mechanisms of RBC alloantibody induction, or strategies to m...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12217
更新日期:2014-01-01 00:00:00
abstract::We report a case of "naturally-occurring" (nonred blood cell stimulated) anti-K1. The patient had never received a blood transfusion. Red blood cell antibody screening panels showed agglutination with K:1 red blood cells at room temperature and not in the anti-globulin test. Testing with 2-mercaptoethanol showed the a...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1978.18478251244.x
更新日期:1978-07-01 00:00:00
abstract:BACKGROUND:Recent retrospective studies suggest myelodysplastic syndromes (MDSs) are more common than previously recognized and patients who develop transfusional dependence may be at risk for increased comorbid complications. STUDY DESIGN AND METHODS:A retrospective review was undertaken of Medicare claims focusing o...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2012.03626.x
更新日期:2012-10-01 00:00:00
abstract:BACKGROUND:Preoperative autologous blood donation (PABD) has been used to reduce the need for allogeneic RBC transfusion, decreasing risk and conserving supply. A frozen PABD program for heart transplant patients was instituted at the Mayo Clinic in 1988, but participation has steadily declined. The aims of this study ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2003.00357.x
更新日期:2003-04-01 00:00:00
abstract:BACKGROUND:Proteomics methods may be used to analyze changes occurring in stored blood products. These data sets can identify processes leading to storage-associated losses of blood component quality such as the platelet (PLT) storage lesion (PSL). The optimal strategy to perform such analyses to obtain the most inform...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2007.01546.x
更新日期:2008-03-01 00:00:00
abstract:BACKGROUND:Antibodies to Mia , MUT, and Mur are among the most frequently identified alloantibodies in Southeast Asia. Understanding the characteristics of these antibodies in terms of induction and evanescence would aid in optimizing methods for their detection. STUDY DESIGN AND METHODS:Antibody testing results betwe...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14538
更新日期:2018-05-01 00:00:00
abstract:BACKGROUND:To minimize the bacterial contamination rate in blood collected from donors, a study was designed to evaluate the suitability of a single-use chlorhexidine-alcohol antiseptic for donor arm preparation at all blood collection venues in Australia. STUDY DESIGN AND METHODS:A prospective study of bacterial load...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2004.03362.x
更新日期:2004-08-01 00:00:00