Abstract:
BACKGROUND:Autologous umbilical cord blood (AutoUCB) has historically been cryopreserved for potential use in hematopoietic transplantation. Increasingly, private AutoUCB banking is performed for therapies unavailable today. A Phase I trial using AutoUCB treatment for early pediatric Type 1 diabetes afforded us an opportunity to analyze characteristics of AutoUCBs. STUDY DESIGN AND METHODS:Twenty AutoUCBs from AABB-accredited private cord blood banks (CBBs) were evaluated for collection, processing, cryopreservation, and thaw characteristics. Using a standardized thaw-wash method, AutoUCBs were assessed for viable total nucleated cells (vTNCs), viable CD34+ (vCD34+), and colony-forming unit-granulocyte-macrophage counts. Postthaw %vTNC recoveries were compared against processing characteristics and analyzed according to processing method, cryopreservation volume, concentration, container, and length of storage. RESULTS:AutoUCB collection volumes (19.9-170 mL), cryopreserved TNC counts (7.6 × 10(7) -3.34 × 10(9)), %TNC processing recoveries (39%-100%), postthaw %vTNC recoveries (58%-100%), and %vCD34+ recoveries (26%-96%) varied widely. Regarding cell dose requirements, only 11% of evaluable AutoUCBs achieved the minimum TNC count of at least 9.0 × 10(8) to meet the National Cord Blood Inventory banking threshold, and only 50% met the minimum of 5.0 × 10(8) TNC count for Food and Drug Administration cord blood licensure eligibility. %vTNC recoveries correlated with %vCD34+ recoveries (R = 0.7; p = 0.03). Length of storage, cryopreservation volume, concentration, and container type did not affect postthaw %vTNC recoveries. CBB processing method, however, was associated with %vTNC postprocessing recoveries, with unmanipulated and plasma-depleted AutoUCBs having the highest postthaw %vTNC recovery, followed by RBC-depleted and density gradient-separated AutoUCBs. CONCLUSION:The high variability and low counts found in AutoUCB banking suggest that further standardization of characterization, collection, and processing procedures is needed.
journal_name
Transfusionjournal_title
Transfusionauthors
Rosenau EH,Sugrue MW,Haller M,Fisk D,Kelly SS,Chang M,Hou W,Eldjerou L,Slayton W,Cogle CR,Wingard JRdoi
10.1111/j.1537-2995.2011.03556.xsubject
Has Abstractpub_date
2012-10-01 00:00:00pages
2234-42issue
10eissn
0041-1132issn
1537-2995journal_volume
52pub_type
杂志文章相关文献
TRANSFUSION文献大全abstract:BACKGROUND:Recombinant activated factor VII (rFVIIa) is being tested to improve hemostasis in a variety of bleeding disorders. Clinical indications and efficacy are still being evaluated for this product. CASE REPORT:Over a 17-month period, rFVIIa was used to treat central nervous system hemorrhage in three patients w...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2004.04080.x
更新日期:2004-11-01 00:00:00
abstract:BACKGROUND:Iron deficiency leading to low hemoglobin concentration (cHb) is a common problem for blood donors as well as for blood banks. A standardized protocol offering iron supplementation based on P-ferritin determination may help to reduce the problem and retain donors. STUDY DESIGN AND METHODS:This was a prospec...
journal_title:Transfusion
pub_type: 临床试验,杂志文章
doi:10.1111/j.1537-2995.2007.01601.x
更新日期:2008-04-01 00:00:00
abstract:BACKGROUND:Almost 20% of parvovirus B19 foetal infections require intrauterine transfusions. In addition, myocardial dysfunction has been observed in severe parvovirus B19 infections. One objective of an intrauterine exchange transfusion (IUET) is to avoid an overload during the transfusion. Our aim was to study the ob...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14968
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND:Antigens of the human blood group system Gerbich (Ge) are located on sialoglycoproteins glycophorin C (GPC) and glycophorin D (GPD). CASE REPORT:The Ge+ proposita (RW) produced an alloanti-Ge after receiving 2 units of red cells (RBCs) during surgery. Further studies were carried out to characterize the ant...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1997.371098016440.x
更新日期:1997-10-01 00:00:00
abstract::Soft and hard-packed red blood cells in four different CPD anticoagulant-preservative solutions were stored with and without added glucose, adenine, and electrolytes. The hemoglobin-oxygen affinity of the red blood cell concentrates was tested over a six-week storage period. No single solution conferred better protect...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1981.21682085761.x
更新日期:1981-11-01 00:00:00
abstract::The plateletpheresis program of the M.D. Anderson Hospital was established in 1963. Approximately 20,000 units of platelet concentrates are produced per year. Credit for the nonreplacement fee generates the greatest number of donors. No organized recruitment campaign exists. Each donor becomes a recruiter for the prog...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1977.17377196353.x
更新日期:1977-05-01 00:00:00
abstract:BACKGROUND:Effective donor skin disinfection is essential in preventing bacterial contamination of blood components with skin flora bacteria like Staphylococcus epidermidis. Cell aggregates of S. epidermidis (biofilms) are found on the skin and are resistant to the commonly used donor skin disinfectants chlorhexidine-g...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14298
更新日期:2017-12-01 00:00:00
abstract:BACKGROUND:The successful mobilization and collection of hematopoietic stem cells are dependent on a number of clinical factors such as previous chemotherapy and disease stage. The aim of this retrospective study was to determine whether the effectiveness of mobilization and collection is an independent prognostic fact...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2007.01448.x
更新日期:2007-12-01 00:00:00
abstract:BACKGROUND:A new automated apheresis system has recently been reported as useful in improving peripheral blood HPC collection in adults. The aim of this study has been to verify the utility of this system (AutoPBSC, COBE BCT) for standard leukapheresis and for LVL in the pediatric setting. STUDY DESIGN AND METHODS:A p...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2000.40040404.x
更新日期:2000-04-01 00:00:00
abstract::Acute trauma with severe bleeding caused the unavoidable transfusion of Rh-positive red cells (RBCs) to a young, childless, Rh-negative woman during surgery. Of the 10 units of RBCs given, 6 were Rh positive. An alternative treatment for large-volume exposure involves the removal of some of the Rh-positive cells trans...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1993.33693296819.x
更新日期:1993-06-01 00:00:00
abstract:BACKGROUND:Hemovigilance has shown that bacteria cause more fatalities than other infections together. Surveillance for detection of bacteria in platelets (PLTs) was initiated. Concomitantly, the storage period for PLTs was extended from 5 to 7 days to reduce cost. STUDY DESIGN AND METHODS:Analysis was performed of al...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2004.04019.x
更新日期:2004-08-01 00:00:00
abstract::Two monoclonal antibodies (MoAbs), BRIC 66 (IgM) and BRIC 111 (IgG1), were produced by immunizing mice with ovarian cyst blood group A1 glycoprotein and Tn red cells (RBCs), respectively. Their specificities were determined by inhibitions using Tn sialoglycoproteins (SGPs), mucins (armadillo [ASG] and ovine [OSG] subm...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1991.31291142945.x
更新日期:1991-02-01 00:00:00
abstract::Three patients with leukemia were treated for acute leukostasis using the continuous-flow cell separator. The first patient, treated with a standard procedure sustained only a 14 per cent decrease in leukocyte count even though 50 X 10(10) leukocytes were removed. A more aggressive approach was taken with the second t...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1980.20480260280.x
更新日期:1980-07-01 00:00:00
abstract::An exchange transfusion was performed preoperatively on a patient with sickle cell disease using a continuous-flow blood cell separator. An exchange of 2,825 ml red blood cells achieved a hemoglobin A level of 90.8 per cent. The continuous-flow blood cell separator appears to offer a safe and effective method of excha...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1977.17377196364.x
更新日期:1977-05-01 00:00:00
abstract:BACKGROUND:Transfusion of platelet concentrates (PCs) is the basic treatment for severe platelet disorders. PCs carry the risk of pathogen transmission, especially bacteria. Pathogen reduction (PR) by addition of photochemical reagents and irradiation with visible or ultraviolet (UV) light can significantly reduce this...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2009.02334.x
更新日期:2009-12-01 00:00:00
abstract:BACKGROUND:Alloimmunization against human platelet antigens (HPAs) during pregnancy is rare but can lead to severe bleeding disorders, such as fetal and neonatal alloimmune thrombocytopenia. STUDY DESIGN AND METHODS:In a cohort of 241 uncomplicated pregnancies, we investigated the immunogenicity of HPA mismatches and ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14053
更新日期:2017-05-01 00:00:00
abstract:BACKGROUND:Research with primary human white blood cell (WBC) subpopulations requires high quantity, quality, and functionality of peripheral blood mononuclear cells (PBMCs) as a source to further characterize cellular subpopulations such as T and B lymphocytes, monocytes, or natural killer cells. Apart from buffy coat...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14616
更新日期:2018-06-01 00:00:00
abstract:BACKGROUND:Routine HCV NAT of blood donors to detect persons in the preseroconversion phase of acute infection was introduced in Canada in October 1999. The source of virus exposure was investigated in the first, and to date only, blood donor found to be HCV NAT positive, anti-HCV negative in Canada. He was a regular d...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2002.00112.x
更新日期:2002-06-01 00:00:00
abstract:BACKGROUND:Interventions intended to motivate donors to return can be costly and time consuming. The current study examined the effect of a Web-based automated interview, informed by motivational interviewing and self-determination theory, on donor intention, motivation, and behavior in a sample of highly experienced d...
journal_title:Transfusion
pub_type: 杂志文章,随机对照试验
doi:10.1111/trf.15402
更新日期:2019-09-01 00:00:00
abstract::In this study we tested the seeds of uncultivated plants for lectin activity. Extracts prepared from the seeds of 167 forb species were tested against human red cell samples. Extracts of 54 species agglutinated unmodified or enzyme-modified red cells. In nine species, the hemagglutinating lectin activity could be neut...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1983.23684074276.x
更新日期:1983-11-01 00:00:00
abstract::A 24-year-old, blood-group O Polynesian woman with multiple red blood cell antibodies received a group A, HLA-compatible, sex-matched bone marrow transplantation as treatment for severe aplastic anemia. High retransplantation titers of ABO isohemagglutinins were successfully reduced by repeated plasmaphereses. Despite...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1982.22182154221.x
更新日期:1982-01-01 00:00:00
abstract:BACKGROUND:Recent retrospective studies indicate that D- recipients of D+ apheresis platelets (PLTs) are not alloimmunized to D. Our hospital policy is to offer RhIG to D- women of childbearing age who received D+ apheresis PLTs but not to other D- recipients of D+ apheresis PLTs. We instituted prospective surveillance...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12972
更新日期:2015-06-01 00:00:00
abstract:BACKGROUND:During the COVID-19 outbreak, most hospitals deferred elective surgical procedures to allow space for the overwhelming number of COVID-19 patient admissions, expecting a decrease in routine blood component requirements. However, because transfusion support needs of COVID-19 patients are not well known, its i...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.16171
更新日期:2020-11-04 00:00:00
abstract::Over a 6-year period, 866 cases were analyzed for disputed parentage. In 194 cases (22%), the alleged father was excluded as the biological father. One hundred and eight-one cases (93%) were direct exclusions, and 13 cases (7%) were indirect exclusions. Ninety-two percent of falsely accused men were identified by typi...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1984.24484275578.x
更新日期:1984-07-01 00:00:00
abstract:BACKGROUND:Chronic red blood cell exchanges (RBCXs) are frequently used to prevent complications in patients with sickle cell anemia, but the scarcity of matched red blood cell packs (RBCPs) is a serious concern. The main goal of this study was to compare the number of RBCPs used during RBCXs between the Spectra Optia ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15188
更新日期:2019-05-01 00:00:00
abstract::The selection of platelet donors, according to HLA antigens, has been computerized using a method based on the calculation of incompatibilities and risk of immunization between donors and recipients. Since the risk that an antigen present in a donor will immunize an incompatible recipient of known HLA phenotype, an in...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1981.21381201795.x
更新日期:1981-05-01 00:00:00
abstract::An example of anti-Cob which caused an acute hemolytic transfusion reaction is reported. Immunohematologic studies showed this antibody to be an IgG immunoglobulin that weakly bound complement and reacted in the antiglobulin phase of the crossmatch. The unit of whole blood implicated in the hemolytic transfusion react...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1982.22282177127.x
更新日期:1982-03-01 00:00:00
abstract:BACKGROUND:Variations in a multipass transmembrane protein may affect its membrane integration. To study this effect, the systematic molecular characterization of variant D antigen density is a suitable model. Unlike most other membrane proteins, the expression of the D antigen is often determined by a single allele, b...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2006.00902.x
更新日期:2006-08-01 00:00:00
abstract:BACKGROUND:A high-throughput detection system was developed for HBV DNA and HCV RNA. METHODS:A combination of real-time detection PCR using an automated system (PRISM 7700, PE Biosystems, Foster City, CA) and automatic viral nucleic acid extraction (BioRobot 9604, Qiagen, Hilden, Germany) was used as the high-throughp...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2002.00024.x
更新日期:2002-01-01 00:00:00
abstract::The ability of the monocyte monolayer assay (MMA) and the chemiluminescence test (CLT) to predict the clinical significance of alloantibodies associated with hemolytic disease of the newborn (HDN) was assessed by the use of 22 well-characterized antisera--predominantly anti-D--from alloimmunized pregnant women. Sevent...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1993.33693296810.x
更新日期:1993-06-01 00:00:00