High-throughput HBV DNA and HCV RNA detection system using a nucleic acid purification robot and real-time detection PCR: its application to analysis of posttransfusion hepatitis.

Abstract:

BACKGROUND:A high-throughput detection system was developed for HBV DNA and HCV RNA. METHODS:A combination of real-time detection PCR using an automated system (PRISM 7700, PE Biosystems, Foster City, CA) and automatic viral nucleic acid extraction (BioRobot 9604, Qiagen, Hilden, Germany) was used as the high-throughput detection system. An internal control for HBV DNA detection was also developed. RESULTS:Testing of 96 samples for HBV and HCV was completed within 5 hours. The sensitivity of this system almost equals that of the manual method using nested PCR. The addition of an internal control for HBV detection did not affect the sensitivity of the method and confirmed the accuracy of results. It was possible to quantify HBV in HBV+ samples that contain more than 500 genome equivalents per mL. We started using this system from June 1999 for testing stored donor and patient samples to analyze cases of posttransfusion hepatitis and identified three HBV+ donations that were implicated in posttransfusion hepatitis B. CONCLUSION:The high-throughput detection system is a useful tool for HBV DNA and HCV RNA detection because it enables rapid and reliable testing of a large number of samples.

journal_name

Transfusion

journal_title

Transfusion

authors

Mitsunaga S,Fujimura K,Matsumoto C,Shiozawa R,Hirakawa S,Nakajima K,Tadokoro K,Juji T

doi

10.1046/j.1537-2995.2002.00024.x

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

100-6

issue

1

eissn

0041-1132

issn

1537-2995

journal_volume

42

pub_type

杂志文章
  • Comparison of antibody elution techniques by enzyme-linked antiglobulin test.

    abstract::The enzyme-linked antiglobulin test was used to determine the percentage of antibody removed from sensitized red cells by five elution methods: Rubin ether, xylene, digitonin-acid, glycine, and heat. Antibodies examined in the study included anti-D, -c, -E, and -K. With two examples of anti-D, more antibody was eluted...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1983.23483276862.x

    authors: Gibble JW,Salamon JL,Ness PM

    更新日期:1983-07-01 00:00:00

  • Impact of different hold time before addition of platelet additive solution on the in vitro quality of apheresis platelets.

    abstract:BACKGROUND:The quality of platelets (PLTs) stored in PLT additive solution (PAS) is dependent on the type and proportion of the used PAS. No data are available as to whether a different hold time before the addition of PAS to hyperconcentrated PLT suspensions has an impact on PLT quality. The in vitro quality between s...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2006.00826.x

    authors: Ringwald J,Haager B,Krex D,Zimmermann R,Strasser E,Antoon M,De Schrijver E,Eckstein R

    更新日期:2006-06-01 00:00:00

  • Identification and quantitation of the protein content of blood group antibody eluates prepared by the rapid acid-stromal method. Use in committed erythroid progenitor cell assays.

    abstract::Anti-D sensitized red cells were converted to stroma, either by digitonin or hypotonic lysis, and eluates prepared by the modified rapid acid-stromal method. SDS-PAGE and both rocket- and crossed-immunoelectrophoresis showed that, of the total protein content of the eluates, 11 to 25 percent was IgG, 18 to 45 percent ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1983.23383224906.x

    authors: Rearden A,Chiu P,Elmajian D

    更新日期:1983-05-01 00:00:00

  • The effects of combined platelet and leukapheresis on the blood coagulation system.

    abstract::Analysis of blood coagulation was done on samples of blood collected from ten donors undergoing combined platelet and leukapheresis using the Haemonetics Model 30 Blood Processor. Blood samples were obtained from the donors prior to, during, and following pheresis. Blood was also obtained from the blood-return line af...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1979.19279160288.x

    authors: Kisker CT,Strauss RG,Koepke JA,Maguire LC,Thompson JS

    更新日期:1979-03-01 00:00:00

  • Fast and low-cost direct ELISA for high-throughput serological HPA-1a typing.

    abstract:BACKGROUND:Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is caused by maternal alloantibodies against fetal human platelet antigens (HPAs), mostly caused by anti-HPA-1a. Population-based screening for FNAIT is still a topic of debate. Logistically and financially, the major challenge for implementation is the ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15454

    authors: Winkelhorst D,Porcelijn L,Muizelaar E,Oldert G,Huiskes E,van der Schoot CE

    更新日期:2019-09-01 00:00:00

  • Mobilization, collection, and immunomagnetic selection of peripheral blood CD34 cells in recovered aplastic anemia patients.

    abstract:BACKGROUND:Most patients with severe aplastic anemia (sAA) respond to immunosuppression, but a significant number relapse or develop clonal abnormalities such as paroxysmal nocturnal hemoglobinuria, myelodysplasia, or leukemia. In principle, patients without matched sibling donors and older patients might benefit from ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2007.01258.x

    authors: Sloand EM,Read EJ,Scheinberg P,Tang Y,More K,Leitman SF,Maciejewski J,Young NS

    更新日期:2007-07-01 00:00:00

  • Blood use in the ambulatory setting among elderly in the United States.

    abstract:BACKGROUND:Our study characterizes blood use in the ambulatory setting by US elderly Medicare beneficiaries during 2001. As the US population ages and delivery of health care in outpatient settings is on the rise, ambulatory blood utilization is expected to increase. There is currently a lack of broad population-based ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2009.02114.x

    authors: Menis M,Burwen DR,Holness L,Anderson SA

    更新日期:2009-06-01 00:00:00

  • In vitro and in vivo quality of leukoreduced apheresis platelets stored in a new platelet additive solution.

    abstract:BACKGROUND:Platelets (PLTs) stored in additive solutions (PASs) may reduce the risk of several plasma-associated adverse transfusion reactions such as allergic reactions and potentially transfusion-associated lung injury. The objective of this study was to determine the in vitro characteristics and the in vivo radiolab...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2012.03841.x

    authors: Dumont LJ,Cancelas JA,Graminske S,Friedman KD,Vassallo RR,Whitley PH,Rugg N,Dumont DF,Herschel L,Siegal AH,Szczepiorkowski ZM,Fender L,Razatos A

    更新日期:2013-05-01 00:00:00

  • Acquisition of cytomegalovirus infection in infants following exchange transfusion: a prospective study.

    abstract::A prospective study of newborn infants who required exchange transfusion was undertaken to evaluate the risk of transmission of cytomegalovirus (CMV). Buffy coat-, urine- and saliva-saturated throat swabs for viral cultures and serum specimens for CMV complement-fixing (CF) antibody were obtained from 45 infant-mother...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1980.20380214901.x

    authors: Kumar A,Nankervis GA,Cooper AR,Gold E,Kumar ML

    更新日期:1980-05-01 00:00:00

  • Successful kidney transplantation in a patient with congenital thrombotic thrombocytopenic purpura (Upshaw-Schulman syndrome).

    abstract:BACKGROUND:Congenital thrombotic thrombocytopenic purpura (TTP) may not be recognized until organ failure related to the microvascular thrombosis occurs. Kidney failure may be the initial presenting clinical feature. Kidney transplantation has been contraindicated because of the assumption that the continuing microvasc...

    journal_title:Transfusion

    pub_type:

    doi:10.1111/trf.14326

    authors: Fattah H,Kumar D,George JN,Massey HD,King AL,Friedman KD,Gupta G

    更新日期:2017-12-01 00:00:00

  • Hemostats, sealants, and adhesives III: a new update as well as cost and regulatory considerations for components of the surgical toolbox.

    abstract::The hemostat, sealant, and adhesive components of the surgical toolbox continue to evolve and enter clinical practice at a rapid rate. The goal of this comprehensive, sequential review is to update these components to include those now available (February 2012) as well as to explore cost and regulatory factors that im...

    journal_title:Transfusion

    pub_type: 杂志文章,评审

    doi:10.1111/j.1537-2995.2012.03707.x

    authors: Spotnitz WD,Burks S

    更新日期:2012-10-01 00:00:00

  • In vivo and in vitro characteristics of double units of RBCs collected by apheresis with a single in-line WBC-reduction filter.

    abstract:BACKGROUND:A novel apheresis procedure for a blood separator (MCS+, Haemonetics) enables the collection of 2 WBC-reduced RBC units in a single donation by using one disposable set with one in-line WBC-reduction filter (RC2H, Pall Corp.). The objective of this study was to evaluate the filtration performance in connecti...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2001.41111373.x

    authors: Bandarenko N,Rose M,Kowalsky RJ,Baston RK,Brecher ME,Elfath MD,Whitley P,Heminway M,Holme S

    更新日期:2001-11-01 00:00:00

  • Diagnostic performance of prothrombin time point-of-care to detect acute traumatic coagulopathy on admission: experience of 522 cases in trauma center.

    abstract:BACKGROUND:Early identification of acute traumatic coagulopathy is a key challenge during initial management to determine whether to initiate early hemostatic support. We assessed the performance of prothrombin time (PT) at point-of-care in trauma patients to detect moderate and severe coagulopathy on admission. STUDY...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.14643

    authors: Deras P,Nouri J,Martinez O,Aubry E,Capdevila X,Charbit J

    更新日期:2018-07-01 00:00:00

  • A study of criteria for blood donor deferral.

    abstract::Donor deferral rates in regional blood centers vary from 5 to 24 per cent, reducing by more than 1,250,000 the number of units of volunteer blood available for transfusion in the nation each year. Those criteria for donor deferral which are intended to exclude donors likely to suffer a "donor reaction" are based parti...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1980.20581034503.x

    authors: Tomasulo PA,Anderson AJ,Paluso MB,Gutschenritter MA,Aster RH

    更新日期:1980-09-01 00:00:00

  • Establishing a reentry procedure for human immunodeficiency virus screening-reactive donors in China.

    abstract:BACKGROUND:Screening of blood donors for antibody to human immunodeficiency virus Types 1 and 2 (anti-HIV-1/2) and/or HIV nucleic acid test (NAT) is a well-established venue to prevent HIV transfusion-transmitted disease. However, with the current available technologies, HIV testing may result in donor loss due to fals...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.13282

    authors: Li L,Xu T,Yang T,Zang L,Cheng W,Lin H,Zhu S,Zhao B,Duan R,Li C,Huang M,Liu Y,Ming C,Xu S,Wang C,Wu Y,Liu Z

    更新日期:2016-01-01 00:00:00

  • A novel GYPB-A-B hybrid gene responsible for Ss and MN typing discrepancies.

    abstract:BACKGROUND:Transfusion support of S-s- patients is very challenging but can now be alleviated by genotyping mutations in the GYPB gene to predict their U- or U+(var) phenotype. However, the phenotype predicted by genotyping does not always correspond to the observed phenotype of red blood cells (RBCs), which requires f...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.13216

    authors: Willemetz A,Nataf J,Peyrard T,Arnaud L

    更新日期:2015-11-01 00:00:00

  • Noninvasive determination of fetal RHD status by examination of cell-free DNA in maternal plasma.

    abstract:BACKGROUND:Cell-free fetal DNA in maternal plasma opens the way for routine risk-free diagnosis of fetal D status of D- mothers. The focus was on accuracy of RHD typing and confirmation of fetal DNA in maternal plasma while RHD was not detected. STUDY DESIGN AND METHODS:Plasma DNA was extracted (by manual and/or autom...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2005.00559.x

    authors: Brojer E,Zupanska B,Guz K,Orziñska A,Kaliñska A

    更新日期:2005-09-01 00:00:00

  • Absorption of anti-Chido from serum using autologous red blood cells coated with homologous C4.

    abstract::A method is described which absorbs anti-Chido from serum without reducing the strength of other alloantibodies. Autologous red blood cells are coated with homologous C4 by a low-ionic-strength method and then used for the absorption. This is a practical method for removing anti-Chido from serum so that underlying all...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1982.22382224951.x

    authors: Ellisor SS,Shoemaker MM,Reid ME

    更新日期:1982-05-01 00:00:00

  • Economic impact on US Medicare of a new diagnosis of myelodysplastic syndromes and the incremental costs associated with blood transfusion need.

    abstract:BACKGROUND:Recent retrospective studies suggest myelodysplastic syndromes (MDSs) are more common than previously recognized and patients who develop transfusional dependence may be at risk for increased comorbid complications. STUDY DESIGN AND METHODS:A retrospective review was undertaken of Medicare claims focusing o...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2012.03626.x

    authors: Goldberg SL,Chen E,Sasane M,Paley C,Guo A,Laouri M

    更新日期:2012-10-01 00:00:00

  • Granulocyte-colony-stimulatory factor: a strong inhibitor of natural killer cell function.

    abstract:BACKGROUND:The human cytokine granulocyte-colony stimulatory factor (G-CSF) has found widespread application in the medical treatment of neutropenia and to mobilize hematopoietic stem cells used for transplantation. So far, the effect of G-CSF on natural killer (NK) cells has not been fully investigated. STUDY DESIGN ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2010.02820.x

    authors: Schlahsa L,Jaimes Y,Blasczyk R,Figueiredo C

    更新日期:2011-02-01 00:00:00

  • Effects of colloidal resuscitation fluids on the neutrophil respiratory burst.

    abstract:BACKGROUND:Experimental studies have revealed that gelatin and HES produce increased neutrophil respiratory burst activity. It was investigated whether 3-percent gelatin (MW 35,000) and three types of 6-percent HES (MW 70,000; degree of substitution, 0.5; 200,000/0.5; 450,000/0.7) preparations can influence superoxide ...

    journal_title:Transfusion

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1046/j.1537-2995.2001.41081064.x

    authors: Jaeger K,Heine J,Ruschulte H,Jüttner B,Scheinichen D,Kuse ER,Piepenbrock S

    更新日期:2001-08-01 00:00:00

  • Flow cytometric determination of RBC survival in autoimmune hemolytic anemia.

    abstract:BACKGROUND:In cases of warm autoimmune hemolytic anemia (WAIHA), crossmatch incompatible RBCs are most often used for transfusion. The determination of the in vivo survival of transfused and autologous RBCs in WAIHA is helpful in the assessment of the efficacy of transfusion and other therapeutic interventions. CASE R...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2001.41040493.x

    authors: Zeiler T,Müller JT,Hasse C,Kullmer J,Kretschmer V

    更新日期:2001-04-01 00:00:00

  • Inactivation of viruses in platelet concentrates by photochemical treatment with amotosalen and long-wavelength ultraviolet light.

    abstract:BACKGROUND:Viral contamination of platelet (PLT) concentrates can result in transfusion-transmitted diseases. A photochemical treatment (PCT) process with amotosalen-HCl and long-wavelength ultraviolet light (UVA), which cross-links nucleic acids, was developed to inactivate viruses and other pathogens in PLT concentra...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.0041-1132.2005.04316.x

    authors: Lin L,Hanson CV,Alter HJ,Jauvin V,Bernard KA,Murthy KK,Metzel P,Corash L

    更新日期:2005-04-01 00:00:00

  • Hemovigilance survey of pathogen-reduced blood components in the Warsaw Region in the 2009 to 2013 period.

    abstract:BACKGROUND:In 2009 the Mirasol Pathogen Reduction Technology (PRT) was introduced to the routine blood component production of the Regional Blood Transfusion Center in Warsaw (RBTCW). The goal of this study was to investigate the safety of Mirasol-treated blood components. STUDY DESIGN AND METHODS:The accumulated pass...

    journal_title:Transfusion

    pub_type: 杂志文章,评审

    doi:10.1111/trf.13330

    authors: Łętowska M,Przybylska Z,Piotrowski D,Lachert E,Rosiek A,Rzymkiewicz L,Cardoso M

    更新日期:2016-03-01 00:00:00

  • UMC, another Cromer-related blood group antigen.

    abstract::An antibody found in the serum of a Japanese blood donor detects a new high-frequency red cell antigen named UMC. UMC is absent from Inab phenotype cells, weakly expressed on Dr(a-) cells, and destroyed by alpha-chymotrypsin but not trypsin treatment of red cells. UMC is, therefore, a Cromer-related antigen. Immunoblo...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1989.29990070184.x

    authors: Daniels GL,Okubo Y,Yamaguchi H,Seno T,Ikuta M

    更新日期:1989-11-01 00:00:00

  • Development of a quality monitoring program for platelet components: a report of the first four years' experience at Canadian Blood Services.

    abstract:BACKGROUND:A quality monitoring program (QMP) for platelet concentrates (PCs) was implemented at Canadian Blood Services (CBS) to improve standards and to better understand platelet (PLT) products by supplementing routine quality control (QC). STUDY DESIGN AND METHODS:Annual surveys of PCs from CBS production sites we...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2011.03402.x

    authors: Levin E,Jenkins C,Culibrk B,Gyöngyössy-Issa MI,Serrano K,Devine DV

    更新日期:2012-04-01 00:00:00

  • Circulating red cells usually remain of host origin after bone marrow transplantation for severe combined immunodeficiency.

    abstract:BACKGROUND:Patients with severe combined immunodeficiency (SCID) treated with allogeneic bone marrow transplantation often receive a milder conditioning regimen than patients who undergo transplantation for hematologic malignancy, and they regularly retain circulating white cells of host origin. The origin of circulati...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1996.36496226143.x

    authors: Brady KA,Cowan MJ,Leavitt AD

    更新日期:1996-04-01 00:00:00

  • A multicenter evaluation of reproducibility of swirling in platelet concentrates. Biomedical Excellence for Safer Transfusion (BEST) Working Party of the International Society of Blood Transfusion.

    abstract:BACKGROUND:Eleven laboratories participated in a study investigating whether swirling can be used in monitoring platelet discoid morphology in platelet concentrates (PCs). STUDY DESIGN AND METHODS:In each laboratory, two readers observed the swirling pattern in 1- to 5-day-old PCs, in PCs containing spherical platelet...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1994.34994378282.x

    authors: Bertolini F,Murphy S

    更新日期:1994-09-01 00:00:00

  • An analysis on the fate of a selection of blood products derived from cytomegalovirus-seronegative donors at three tertiary referral hospitals in Australia.

    abstract:BACKGROUND:Supply of cytomegalovirus (CMV)-seronegative blood products in Australia is an ongoing challenge. Requests for CMV-negative products are increasing with prediction that the demand will exceed supply by 2019. Clinical information evaluating how these products are being utilized by health providers within Aust...

    journal_title:Transfusion

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1111/trf.14459

    authors: Hirani R,Tohidi-Esfahani I,Mondy P,Irving DO

    更新日期:2018-03-01 00:00:00

  • Liquid storage at 4 degrees C of previously frozen red cells.

    abstract::Fresh human blood was collected in citrate-phosphate-dextrose, frozen by a high-glycerol technique, and stored at -80 degrees C. The red cells were thawed, deglycerolized, and resuspended in a final wash solution, ADSOL (Fenwal Laboratories), or an additive solution (AS) containing glucose, adenine, mannitol, and phos...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1987.27688071704.x

    authors: Moore GL,Ledford ME,Mathewson PJ,Hankins DJ

    更新日期:1987-11-01 00:00:00