Detection of bacterial contamination of platelet components: six years' experience with the BacT/ALERT system.


BACKGROUND:Hemovigilance has shown that bacteria cause more fatalities than other infections together. Surveillance for detection of bacteria in platelets (PLTs) was initiated. Concomitantly, the storage period for PLTs was extended from 5 to 7 days to reduce cost. STUDY DESIGN AND METHODS:Analysis was performed of all cases of a positive signal in a screening procedure for contaminated PLTs taking into account results of confirmative cultures and results of culture from blood components including bacteria strains. Records were assessed from patients transfused with blood components issued before the screening culture became positive. RESULTS:Samples were collected from 22,057 PLT units. An initial reaction was seen in 84 (0.38%). Growth was confirmed in 70 of these. Of the associated PLT units, 26 had been issued or outdated at the time when the culture was found to be reactive, in 27 bacteria were found, and in 17 cultures were negative. The bacteria found were mainly from normal skin flora. Sixty-six patients received 75 blood components issued before the screening system alarmed. None of these patients had a transfusion reaction reported. The outdating fell to less than 5 percent. CONCLUSION:A screening system for detection of bacterial contamination was implemented without increase in cost owing to extension of storage time to 7 days. Transfusion of several contaminated blood components was prevented.






Munksgaard L,Albjerg L,Lillevang ST,Gahrn-Hansen B,Georgsen J




Has Abstract


2004-08-01 00:00:00














  • Impact of leukapheresis on early death rate in adult acute myeloid leukemia presenting with hyperleukocytosis.

    abstract:BACKGROUND:Patients with acute myeloid leukemia (AML) with hyperleukocytosis of at least 100 x 10(9) per L are at high risk of early death due to pulmonary or cerebral leukostasis. Although the efficacy of leukapheresis in terms of prompt cytoreduction is generally accepted, published data regarding the clinical value ...


    pub_type: 杂志文章


    authors: Bug G,Anargyrou K,Tonn T,Bialleck H,Seifried E,Hoelzer D,Ottmann OG

    更新日期:2007-10-01 00:00:00

  • Extension of platelet shelf life with an improved bacterial testing algorithm.

    abstract:BACKGROUND:At Canadian Blood Services, platelet concentrate (PC) shelf life was extended to 7 days with a large-volume, delayed-sampling bacterial screening algorithm. We present the development study and postimplementation results. STUDY DESIGN AND METHODS:In the development study, PCs inoculated with five bacteria (...


    pub_type: 杂志文章


    authors: Ramirez-Arcos S,Evans S,McIntyre T,Pang C,Yi QL,DiFranco C,Goldman M

    更新日期:2020-12-01 00:00:00

  • Importance of ionized magnesium measurement for monitoring of citrate-anticoagulated plateletpheresis.

    abstract:BACKGROUND:The infusion of citrate during apheresis may affect the levels of ionized magnesium in the blood. Hypomagnesemia and concomitant hypocalcemia could influence the parathormone response and could be responsible for some of the symptoms observed during apheresis. STUDY DESIGN AND METHODS:The study reports meas...


    pub_type: 杂志文章


    authors: Mercan D,Bastin G,Lambermont M,Dupont E

    更新日期:1997-04-01 00:00:00

  • An acute hemolytic transfusion reaction caused by an anti-P1 that reacted at 37 degrees C.

    abstract:BACKGROUND:Hemolytic transfusion reactions (HTRs) due to anti-P1 have rarely been reported. There is only one report (from 1945) of an acute HTR due to anti-P1. CASE REPORT:A 74-year-old woman with anti-P1 was given blood that had been found to be compatible by the use of prewarmed serum and saline-suspended red cells...


    pub_type: 杂志文章


    authors: Arndt PA,Garratty G,Marfoe RA,Zeger GD

    更新日期:1998-04-01 00:00:00

  • Bartonella henselae transmission by blood transfusion in mice.

    abstract:BACKGROUND:Bartonella spp. are neglected fastidious Gram-negative bacilli. We isolated Bartonella henselae from 1.2% of 500 studied blood donors and demonstrated that the bacteria remain viable in red blood cell units after 35 days of experimental infection. Now, we aim to evaluate the possibility of B. henselae transm...


    pub_type: 杂志文章


    authors: Silva MN,Vieira-Damiani G,Ericson ME,Gupta K,Gilioli R,de Almeida AR,Drummond MR,Lania BG,de Almeida Lins K,Soares TC,Velho PE

    更新日期:2016-06-01 00:00:00

  • The risk of transfusion-transmitted infections in sub-Saharan Africa.

    abstract:BACKGROUND:Blood transfusions carry the risk of transmitting infections. This risk has been studied in detail in high-income countries but not in sub-Saharan Africa. This study estimates the risks of acquiring human immunodeficiency virus (HIV), hepatitis B virus (HBV), or hepatitis C virus (HCV) from a single unit of ...


    pub_type: 杂志文章,评审


    authors: Jayaraman S,Chalabi Z,Perel P,Guerriero C,Roberts I

    更新日期:2010-02-01 00:00:00

  • Electronic enhancements to blood ordering reduce component waste.

    abstract:BACKGROUND:The effect on component wastage after enhancing the clinician's ability to order blood products in the computerized physician order entry (CPOE) system was investigated in a multihospital network. METHODS:A novel field was added to the CPOE of eight hospitals within a health care system allowing the physici...


    pub_type: 杂志文章


    authors: Yazer MH,Deandrade DS,Triulzi DJ,Wisniewski MK,Waters JH

    更新日期:2016-03-01 00:00:00

  • Predonation hematocrit measurement by whole blood electrical conductivity assay.

    abstract::The whole blood electrical conductivity method for predonation hematocrit determinations was studied. Forty capillary and venous blood samples were tested concurrently with electrical conductivity and centrifugation methods. The electrical conductivity method demonstrated acceptable accuracy but was less precise than ...


    pub_type: 杂志文章


    authors: Schifman RB,Rivers SL,Delduca M

    更新日期:1985-05-01 00:00:00

  • Evaluation and improvement of blood donor educational materials: results from a multicenter randomized controlled trial.

    abstract:BACKGROUND:Blood collection centers are charged with creating donor educational materials (DnEM) that are easily understood across all prospective donor populations, while addressing mandates and recommendations from regulatory agencies and professional standard setting organizations. Donors must have sufficient inform...


    pub_type: 杂志文章


    authors: Wehrli G,Rossmann SN,Waxman DA,Katz LM

    更新日期:2020-08-01 00:00:00

  • Depletion of white cells from platelet concentrates with a new adsorption filter.

    abstract::Removal of white cells (WBCs) from platelets may reduce alloimmunization to WBC antigens, prevent febrile reactions, and improve platelet increments in multiply transfused patients receiving HLA-matched platelets. A new surface-modified fibrous polyester filter was evaluated; it requires no special processing of poole...


    pub_type: 杂志文章


    authors: Kickler TS,Bell W,Drew H,Pall D

    更新日期:1989-06-01 00:00:00

  • Posttransfusion graft-versus-host disease in Japanese newborns.

    abstract:BACKGROUND:Posttransfusion graft-versus-host disease (PT-GVHD) is underdiagnosed and underreported. Risk factors predisposing to PT-GVHD in newborn recipients, the clinical manifestations of the disease in newborns, and its mechanism are not well characterized. STUDY DESIGN AND METHODS:A literature review identified 2...


    pub_type: 杂志文章,评审


    authors: Ohto H,Anderson KC

    更新日期:1996-02-01 00:00:00

  • Adoptive therapy by transfusing expanded donor murine natural killer T cells can suppress acute graft-versus-host disease in allogeneic bone marrow transplantation.

    abstract:BACKGROUND:Invariant natural killer T cells (iNKT cells) may suppress graft-versus-host disease (GVHD) after allogeneic transplantation. The purpose of this study was to investigate the therapeutic potential of iNKT cells from major histocompatibility complex (MHC)-mismatched donors for preventing GVHD after allogeneic...


    pub_type: 杂志文章


    authors: Yang J,Gao L,Liu Y,Ren Y,Xie R,Fan H,Qian K

    更新日期:2010-02-01 00:00:00

  • Xenotropic murine leukemia virus-related virus does not pose a risk to blood recipient safety.

    abstract:BACKGROUND:When xenotropic murine leukemia virus-related virus (XMRV) was first reported in association with chronic fatigue syndrome, it was suggested that it might offer a risk to blood safety. Thus, the prevalence of the virus among blood donors and, if present, its transmissibility by transfusion need to be defined...


    pub_type: 杂志文章


    authors: Dodd RY,Hackett J Jr,Linnen JM,Dorsey K,Wu Y,Zou S,Qiu X,Swanson P,Schochetman G,Gao K,Carrick JM,Krysztof DE,Stramer SL

    更新日期:2012-02-01 00:00:00

  • Metabolic impact of red blood cell exchange with rejuvenated red blood cells in sickle cell patients.

    abstract:BACKGROUND:Red blood cell exchange (RCE) transfusions are a mainstay in the treatment of sickle cell anemia (SCA), and allow a temporary restoration of physiological parameters with respect to erythrocyte oxygen carrying capacity and systems metabolism. Recently, we noted that 1) RCE significantly impacts recipients' m...


    pub_type: 杂志文章


    authors: Gehrke S,Shah N,Gamboni F,Kamyszek R,Srinivasan AJ,Gray A,Landrigan M,Welsby I,D'Alessandro A

    更新日期:2019-10-01 00:00:00

  • The influence of irradiation on stored platelets.

    abstract::Platelet concentrates intended for transfusion to immunosuppressed patients are irradiated to minimize transfusion-induced graft-versus-host disease. Because few reports describe how irradiation influences stored platelets, the authors studied whether 5000 rad of gamma irradiation, the maximum dose currently used clin...


    pub_type: 杂志文章


    authors: Moroff G,George VM,Siegl AM,Luban NL

    更新日期:1986-09-01 00:00:00

  • A whole genome approach for discovering the genetic basis of blood group antigens: independent confirmation for P1 and Xga.

    abstract:BACKGROUND:Although P1 and Xga are known to be associated with the A4GALT and XG genes, respectively, the genetic basis of antigen expression has been elusive. Recent reports link both P1 and Xga expression with nucleotide changes in the promotor regions and with antigen-negative phenotypes due to disruption of transcr...


    pub_type: 杂志文章


    authors: Lane WJ,Aguad M,Smeland-Wagman R,Vege S,Mah HH,Joseph A,Blout CL,Nguyen TT,Lebo MS,Sidhu M,Lomas-Francis C,Kaufman RM,Green RC,Westhoff CM,MedSeq Project.

    更新日期:2019-03-01 00:00:00

  • Reducing reactions to blood donation with applied muscle tension: a randomized controlled trial.

    abstract:BACKGROUND:Unpleasant blood donation-related symptoms may discourage otherwise healthy, altruistic individuals from becoming repeat donors. This study examined a behavioral technique called applied muscle tension (AMT) that might reduce reactions. STUDY DESIGN AND METHODS:A total of 605 donors at mobile clinics were a...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Ditto B,France CR,Lavoie P,Roussos M,Adler PS

    更新日期:2003-09-01 00:00:00

  • Concurrent comparison of the safety of paid cytapheresis and volunteer whole-blood donors.

    abstract:BACKGROUND:Historically, paid blood donors were found to transmit hepatitis at higher rates than volunteers. In those older studies, paid donors frequently were recruited from prisons or slum areas--a finding consistent with the belief that monetary payment in itself did not necessarily lead to the high-risk status of ...


    pub_type: 杂志文章


    authors: Strauss RG,Ludwig GA,Smith MV,Villhauer PJ,Randels MJ,Smith-Floss A,Koerner TA

    更新日期:1994-02-01 00:00:00

  • Rare detection of hepatitis B and hepatitis C virus genomes by polymerase chain reaction in seronegative donors with elevated alanine aminotransferase.

    abstract:BACKGROUND:Since screening for antibody to hepatitis C virus (HCV) was introduced in 1990, posttransfusion hepatitis has been reduced to nearly background levels. This has led to reconsideration of the value of testing donated blood for elevated alanine aminotransferase (ALT). The contribution of ALT testing in detecti...


    pub_type: 杂志文章


    authors: Sankary TM,Yang G,Romeo JM,Ulrich PP,Busch MP,Rawal BD,Vyas GN

    更新日期:1994-08-01 00:00:00

  • DAK, a new low-incidence antigen in the Rh blood group system.

    abstract:BACKGROUND:Some low-incidence antigens in the Rh blood group system (e.g., VS, Rh32, FPTT) are expressed by more than one Rh complex. We describe a new low-incidence antigen that is present on RBCs with the partial D phenotypes, DIIIa or DOL, on RN RBCs and on one example of STEM+S RBCs. STUDY DESIGN AND METHODS:Stand...


    pub_type: 杂志文章


    authors: Reid ME,Storry JR,Sausais L,Tossas E,Rios M,Hue-Roye K,Gloster ES,Miller ST,Wolf C,Lomas-Francis C

    更新日期:2003-10-01 00:00:00

  • Anti-D alloimmunization after D-mismatched allogeneic hematopoietic stem cell transplantation in patients with hematologic diseases.

    abstract:BACKGROUND:The de novo development of anti-D after D-mismatched allogeneic hematopoietic stem cell transplantation (AHSCT) is a possibility that must be considered. The transfusion of D- blood components after AHSCT has been recommended but anti-D alloimmunization in this setting has been studied little. Thus, the aim ...


    pub_type: 杂志文章


    authors: Cid J,Lozano M,Fernández-Avilés F,Carreras E,Pereira A,Mazzara R,Ordinas A

    更新日期:2006-02-01 00:00:00

  • Rapid identification of microorganisms from platelet concentrates by matrix-assisted laser desorption ionization time-of-flight mass spectrometry after short-term incubation on liquid medium.

    abstract:BACKGROUND:Platelets (PLTs) are especially affected by the risk of bacterial contamination. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) is an accurate method for the routine identification of bacterial isolates in microbiology laboratories. We directly applied the MALDI-TOF method to bacteria...


    pub_type: 杂志文章


    authors: Chetouane Y,Dubourg G,Gallian P,Flaudrops C,Chiaroni J,Chabrière E,Raoult D,Camoin-Jau L

    更新日期:2018-03-01 00:00:00

  • Fast and low-cost direct ELISA for high-throughput serological HPA-1a typing.

    abstract:BACKGROUND:Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is caused by maternal alloantibodies against fetal human platelet antigens (HPAs), mostly caused by anti-HPA-1a. Population-based screening for FNAIT is still a topic of debate. Logistically and financially, the major challenge for implementation is the ...


    pub_type: 杂志文章


    authors: Winkelhorst D,Porcelijn L,Muizelaar E,Oldert G,Huiskes E,van der Schoot CE

    更新日期:2019-09-01 00:00:00

  • Solid-phase ABO grouping and Rh typing.

    abstract::A solid-phase adherence method (SPAM) for ABO grouping and Rh typing of red cells (RBCs) has been developed. Adherence reactions were read spectrophotometrically and interpreted by a computer. The SPAM had a 99.6 percent correlation with conventional microplate agglutination methods for ABO grouping and Rh typing. The...


    pub_type: 杂志文章


    authors: Sinor LT,Rachel JM,Beck ML,Bayer WL,Coenen WM,Plapp FV

    更新日期:1985-01-01 00:00:00

  • Transfusion-related adverse reactions reported to the National Healthcare Safety Network Hemovigilance Module, United States, 2010 to 2012.

    abstract:BACKGROUND:In 2010, health care facilities in the United States began voluntary enrollment in the National Healthcare Safety Network (NHSN) Hemovigilance Module. Participants report transfusion practices; red blood cell, platelet (PLT), plasma, and cryoprecipitate units transfused; and transfusion-related adverse react...


    pub_type: 杂志文章


    authors: Harvey AR,Basavaraju SV,Chung KW,Kuehnert MJ

    更新日期:2015-04-01 00:00:00

  • First report of using low-titer cold-stored type O whole blood in massive postpartum hemorrhage.

    abstract:BACKGROUND:In cases of massive hemorrhage in the US military, improved outcomes have been reported with the use of warm, fresh whole blood transfusions. Cold-stored low-titer type O whole blood (LTOWB) has become the preferred product for resuscitation of severe bleeding in deployed surgical units. Reports of LTOWB use...




    authors: Bahr TM,DuPont TL,Morris DS,Pierson SE,Esplin MS,Brown SM,O'Brien EA,Ilstrup SJ,Christensen RD

    更新日期:2019-10-01 00:00:00

  • Red blood cell product utilization in patients undergoing allogeneic stem cell transplantation.

    abstract:BACKGROUND:The risk of transfusion reactions (TR) and the cost of blood has led to efforts to reduce blood use. We changed our practice to transfuse just one instead of two units of red blood cells (RBC) when hemoglobin ≤8 g/dL due to patient blood management (PBM) recommendations. METHODS AND MATERIALS:We compared RB...


    pub_type: 杂志文章


    authors: Gastecki K,Shanley R,Welbig J,Cohn C,Brunstein CG

    更新日期:2019-07-01 00:00:00

  • The minimum number of cord blood units needed for Koreans is 51,000.

    abstract:BACKGROUND:The inventory size for cord blood (CB) depends on the ethnic diversity of human leukocyte antigen (HLA) and the size estimation is important for public health in each ethnicity. STUDY DESIGN AND METHODS:We estimate the CB inventory size in Koreans with stored CB units (CBUs) and patients who underwent allog...


    pub_type: 杂志文章


    authors: Yoon JH,Oh S,Shin S,Park JS,Roh EY,Song EY,Park MH,Han KS

    更新日期:2014-03-01 00:00:00

  • Increasing the economic efficacy of peripheral blood progenitor cell collections by monitoring peripheral blood CD34+ concentrations.

    abstract:BACKGROUND:After mobilization, the collection of peripheral blood progenitor cells (PBPCs) can either be started a fixed number of days after having passed the white blood cell nadir (fixed-day scheme) or be based on monitoring of CD34+ cells. This study was conducted to compare both approaches and to assess possible f...


    pub_type: 杂志文章


    authors: Gutensohn K,Magens MM,Kuehnl P,Zeller W

    更新日期:2010-03-01 00:00:00

  • Improving platelet supply chains through collaborations between blood centers and transfusion services.

    abstract:BACKGROUND:Blood centers and hospital transfusion services are challenged with maintaining an adequate platelet (PLT) inventory to minimize the number of outdated units without risking a major shortage. A novel approach to inventory management was established at our institution through a collaboration between the Stanf...


    pub_type: 杂志文章


    authors: Fontaine MJ,Chung YT,Rogers WM,Sussmann HD,Quach P,Galel SA,Goodnough LT,Erhun F

    更新日期:2009-10-01 00:00:00