Abstract:
:Flow cytometric analysis was performed on red cells (RBCs) to compare the antigen activity of various Rh phenotypes, including the rare variants Del and Du (both high-grade and low-grade), whose genotypes were confirmed or presumed by the family study. This appears to be the first report that the Del phenotype is due to the inhibitory effect of Cde. This study also compares the quantity of the D antigen in different phenotypes, whose genotypes comprise the same Du gene (cDuE) transmitted in a family. RBCs were stained by the indirect immunofluorescence method using IgG anti-D purified through a protein-A affinity column. The mean fluorescence intensity obtained for each genotype representing the activity of the D antigen decreased in the following order (percentages show relative fluorescence intensity taking cDE/cDE control as 100% and Cde/cde as 0%): CDe/cDE, 76 percent; CDe/cde, 73 percent; cDE/Cde, 70 percent; cDE/cDuE, 68 percent; CDe/Cde (high-grade Du), 59 percent; cDuE/cde (low-grade Du), 6 percent; and cDuE/Cde (Del), 3 to 4 percent.
journal_name
Transfusionjournal_title
Transfusionauthors
Hasekura H,Ota M,Ito S,Hasegawa Y,Ichinose A,Fukushima H,Ogata Hdoi
10.1046/j.1537-2995.1990.30390194344.xsubject
Has Abstractpub_date
1990-03-01 00:00:00pages
236-8issue
3eissn
0041-1132issn
1537-2995journal_volume
30pub_type
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