Abstract:
:Disuse osteoporosis, which occurs commonly in prolonged bed rest and immobilization, is becoming a major problem in modern societies; however, the molecular mechanisms underlying unloading-driven bone loss have not been fully elucidated. The osteocyte network is considered to be an ideal mechanosensor and mechanotransduction system. We searched for the molecules responsible for disuse osteoporosis using BCL2 transgenic mice, in which the osteocyte network was disrupted. Pyruvate dehydrogenase kinase 4 (Pdk4), which inactivates pyruvate dehydrogenase complex (PDC), was upregulated in femurs and tibiae of wild-type mice but not of BCL2 transgenic mice after tail suspension. Bone in Pdk4(-/-) mice developed normally and was maintained. At unloading, however, bone mass was reduced due to enhanced osteoclastogenesis and Rankl expression in wild-type mice but not in Pdk4(-/-) mice. Osteoclast differentiation of Pdk4(-/-) bone marrow-derived monocyte/macrophage lineage cells (BMMs) in the presence of M-CSF and RANKL was suppressed, and osteoclastogenesis was impaired in the coculture of wild-type BMMs and Pdk4(-/-) osteoblasts, in which Rankl expression and promoter activity were reduced. Further, introduction of Pdk4 into Pdk4(-/-) BMMs and osteoblasts enhanced osteoclastogenesis and Rankl expression and activated Rankl promoter. These findings indicate that Pdk4 plays an important role in bone loss at unloading by promoting osteoclastogenesis.
journal_name
Bonejournal_title
Boneauthors
Wang Y,Liu W,Masuyama R,Fukuyama R,Ito M,Zhang Q,Komori H,Murakami T,Moriishi T,Miyazaki T,Kitazawa R,Yoshida CA,Kawai Y,Izumi S,Komori Tdoi
10.1016/j.bone.2011.07.012subject
Has Abstractpub_date
2012-01-01 00:00:00pages
409-19issue
1eissn
8756-3282issn
1873-2763pii
S8756-3282(11)01114-8journal_volume
50pub_type
杂志文章相关文献
BONE文献大全abstract::This study describes the early interface reaction of cancellous bone to a nanocrystalline hydroxyapatite cement containing type I collagen (HA/Coll) and its modifications with sodium citrate (CI), calcium carbonate (CA), phosphoserine (P) and phosphoserine plus RGD-peptide (RGD). Cylindrical implants of HA/Coll and it...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2006.11.019
更新日期:2007-04-01 00:00:00
abstract::The cartilage- and bone-forming properties of the periosteum have long since been recognized. As one of the major sources of skeletal progenitor cells, the periosteum plays a crucial role not only in bone development and growth, but also during bone fracture healing. Aided by the continuous expansion of tools and tech...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2014.08.007
更新日期:2015-01-01 00:00:00
abstract::Several Biophosphonates have been used as therapeutic agents for Paget's bone disease. (Chloro-4 phenyl)thiomethylene-bisphosphonate (CIPsMBP) has recently been shown to have significant antiosteoclastic activity while an affect of CIPsMBP on mineralization was only observed at high doses. We tested this drug for 6 mo...
journal_title:Bone
pub_type: 临床试验,杂志文章
doi:10.1016/8756-3282(88)90115-9
更新日期:1988-01-01 00:00:00
abstract::Osteocytes are suggested to play a central role in bone remodeling. Evaluation of iliac crest biopsies is a standard procedure for evaluating bone conditions in the clinical setting. Despite the widespread use of such biopsies, little is known about the population of osteocytes in the iliac crest from normal individua...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2016.07.003
更新日期:2016-10-01 00:00:00
abstract::Ossification of the posterior longitudinal ligament of the spine (OPLL) is characterized by ectopic bone formation in the spinal ligaments. Mechanical stress, which acts on the posterior ligaments, is thought to be an important factor in the progression of OPLL. To elucidate this mechanism, we investigated the effects...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(03)00204-7
更新日期:2003-10-01 00:00:00
abstract::Osteoarthritis (OA) occurs naturally in cynomolgus macaques. The purposes of the present study were to: 1) develop histological measurement schemes to measure the cross-sectional area of axial and abaxial osteophytes in the proximal tibia; 2) determine the effects of long-term estrogen replacement therapy (ERT) on ost...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2007.04.175
更新日期:2007-08-01 00:00:00
abstract::This study aimed to explore the relationships of several indicators of cigarette smoking habits (smoking status, pack-years, age at smoking initiation and smoking cessation) with quantitative computed tomographic (QCT)-derived proximal femur bone measures (trabecular vBMD, integral vBMD and the ratio of cortical to to...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2018.01.014
更新日期:2018-03-01 00:00:00
abstract::The interrelationship between mediators of hormone action (cAMP and calcium) and induction of ornithine decarboxylase (ODC) activity was investigated in bone and bone cells. Stimuli that enhanced the cAMP level in both osteoblastlike (BL) and osteoclastlike (CL) cells and in intact calvaria only induced ODC activity i...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(85)90405-3
更新日期:1985-01-01 00:00:00
abstract::Skeletal disorders in rapidly growing poultry are commonplace. This study was performed to investigate the effect of ornithine alpha-ketoglutarate (OKG) administration during the last 7 weeks of life on structural properties, mineralization, and mechanical endurance of skeleton in turkeys at slaughter. Healthy HB Medi...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2005.12.016
更新日期:2006-07-01 00:00:00
abstract::Bone modeling, the process that continually adjusts bone strength in response to prevalent muscle-loading forces throughout an individual's lifespan, may play an important role in bone fragility with age. Femoral stress, an index of bone modeling response, can be estimated using measurements of DXA derived bone geomet...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2016.06.012
更新日期:2016-09-01 00:00:00
abstract::We have previously shown that the therapeutic range of ultrasound heals osteoradionecrotic bone and induces bone formation in vitro. It is well established that nitric oxide (NO) and prostaglandins are crucial early mediators in mechanically induced bone formation. The therapeutic range of ultrasound may act in the sa...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(02)00789-5
更新日期:2002-07-01 00:00:00
abstract::High-resolution peripheral quantitative computed tomography (HR-pQCT) is clinically available today and provides a non-invasive measure of 3D bone geometry and micro-architecture with unprecedented detail. In combination with microarchitectural finite element (μFE) models it can be used to determine bone strength usin...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.02.022
更新日期:2011-06-01 00:00:00
abstract::In 979 healthy women, aged 30-75 years, bone mass was measured by DXA in the lumbar spine and proximal femur, and by SXA in the distal forearm. Bone turnover was assessed by urinary CrossLaps (CrossLaps ELISA), a new assay which measures type I collagen degradation products in urine and by osteocalcin (two-site N-Mid ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(96)00201-3
更新日期:1996-09-01 00:00:00
abstract::We proposed a novel surface modification for an artificial hip joint stem from the viewpoint of maintenance and establishment of appropriate bone function and microstructure, represented by the preferred alignment of biological apatite (BAp) and collagen (Col). Oriented grooves were introduced into the proximal medial...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2012.11.005
更新日期:2013-02-01 00:00:00
abstract::Paramagnetic manganese can be employed as a calcium surrogate to sensitize the magnetic resonance imaging (MRI) technique to the processing of calcium during the bone formation process. At low doses, after just 48h of exposure, osteoblasts take up sufficient quantities of manganese to cause marked reductions in the wa...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.02.014
更新日期:2011-05-01 00:00:00
abstract::Several studies have shown that treatment with bisphosphonates can reduce the pain associated with different painful diseases. In a previous study we demonstrated that in mice two bisphosponates, clodronate and pamidronate, had an antinociceptive effect under acute conditions not related to bone processes, after in ve...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(03)00229-1
更新日期:2003-10-01 00:00:00
abstract::Whether glucocorticoids directly enhance or interrupt osteoclastogenesis is still a controversial subject. In this study, we ascertained the dose-dependent positive effects of glucocorticoids on osteoclastogenesis in vivo and in vitro as well as investigated the mechanism in vitro. As the dose of glucocorticoids incre...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2015.06.014
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND:U.S. Army Basic Combat Training (BCT) is a physically-demanding program at the start of military service. Whereas animal studies have shown that increased mechanical loading rapidly alters bone structure, there is limited evidence of changes in bone density and structure in humans exposed to a brief period o...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2018.04.021
更新日期:2018-08-01 00:00:00
abstract:INTRODUCTION:The clinically known importance of patient sex as a major risk factor for compromised bone healing is poorly reflected in animal models. Consequently, the underlying cellular mechanisms remain elusive. Because mesenchymal stem cells (MSCs) are postulated to regulate tissue regeneration and give rise to ess...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2009.08.005
更新日期:2009-12-01 00:00:00
abstract::The hypocalcemic and hypophosphatemic effect of salmon calcitonin (sCT) given by intranasal (i.n.) spray to 12 patients with histological confirmed primary hyperparathyroidism (1 degree HPT) was studied. The concentration of ionized calcium in whole blood (B-Ca++), serum phosphate (S-P), magnesium (S-Mg), plasma sCT (...
journal_title:Bone
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/8756-3282(91)90016-c
更新日期:1991-01-01 00:00:00
abstract::The incidence of hip fractures in Oslo has shown a secular increase during the past decades. The main aims of the present study were to report the current incidence of hip fractures in Oslo and to determine whether there is a seasonal variation in the occurrence of fractures. Using the electronic diagnosis registers a...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(01)00603-2
更新日期:2001-11-01 00:00:00
abstract::The assessment of fracture risk often relies primarily on measuring bone mineral density, thereby accounting for only a single pathology: the loss of bone mass. However, bone's ability to resist fracture is a result of its biphasic composition and hierarchical structure that imbue it with high strength and toughness. ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2016.03.013
更新日期:2016-06-01 00:00:00
abstract::Proteins are an essential part of essentially all biological processes, and there is enormous variation in protein forms and concentrations that is not reflected in DNA or RNA. Recently there have been rapid advances in the ability to measure protein sequence, modification and concentration, particularly with methods ...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2019.03.032
更新日期:2019-09-01 00:00:00
abstract:BACKGROUND:Preterm infants are at risk for impaired bone mineralization and growth in length later in life due to inadequate nutritional intake in the early postnatal period. OBJECTIVE:To investigate whether increased nutritional supplementation of calcium, phosphate and protein in Very Low Birth Weight (VLBW) infants...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2017.12.022
更新日期:2018-03-01 00:00:00
abstract::The effects of imaging parameters and special configuration of objects within the reconstruction space on the micro computed tomography (μCT) based mineral density have been explored, and a series of density correction curves have been presented. A manufacturer-provided calibration phantom (0, 100, 200, 400, 800 mg HA...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.10.004
更新日期:2012-03-01 00:00:00
abstract::High bone mass (HBM), detected in 0.2% of DXA scans, is characterised by a mild skeletal dysplasia largely unexplained by known genetic mutations. We conducted the first systematic assessment of the skeletal phenotype in unexplained HBM using pQCT in our unique HBM population identified from screening routine UK NHS D...
journal_title:Bone
pub_type: 杂志文章,多中心研究
doi:10.1016/j.bone.2012.10.021
更新日期:2013-01-01 00:00:00
abstract::It is well known that porosity has an inverse relationship with the mechanical properties of bones. We examined cortical and trabecular porosity of antlers, and mineral composition, thickness and mechanical properties in the cortical wall. Samples belonged to two deer populations: a captive population of an experiment...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.10.026
更新日期:2012-01-01 00:00:00
abstract::The objective of the study was to assess the short- and long-term effects of adhesive capsulitis (frozen shoulder) on the bone mineral density (BMD) of the affected extremity. BMD and clinical status of 22 patients (group A) with active-phase unilateral adhesive capsulitis and 31 patients (group B) with a previous adh...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(98)00049-0
更新日期:1998-06-01 00:00:00
abstract::In order to assess the ability of six potential bone graft substitutes to support the growth of human osteoblasts, these cells were grown in culture and then plated onto fragments of the six materials and cultured for a further period of 15 days. Tests to confirm the osteoblastic phenotype of the cells included spectr...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(93)90089-s
更新日期:1993-07-01 00:00:00
abstract::Bisphosphonate (BP)-related osteonecrosis of the jaw, previously known as BRONJ, now referred to more broadly as medication-related osteonecrosis of the jaw (MRONJ), is a morbid condition that represents a significant risk for oncology patients who have received high dose intravenous (IV) infusion of a potent nitrogen...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.03.027
更新日期:2019-06-01 00:00:00