Abstract：
:Whether glucocorticoids directly enhance or interrupt osteoclastogenesis is still a controversial subject. In this study, we ascertained the dose-dependent positive effects of glucocorticoids on osteoclastogenesis in vivo and in vitro as well as investigated the mechanism in vitro. As the dose of glucocorticoids increased, osteoclastogenesis was stimulated at 0.1 μM, a peak was achieved at 1 μM and a corresponding decrease occurred at 10 μM. Reactive oxygen species (ROS), which play a crucial role in osteoclastogenesis, and autophagy flux activity, a cellular recycling process, were consistently up-regulated along with the dose-dependent effects of the glucocorticoids on osteoclast formation and function. N-acetyl-cysteine (NAC), a ROS scavenger, abrogated the effects of the glucocorticoids on autophagy and osteoclastogenesis. Moreover, 3-methyladenine (3-MA), an autophagy inhibitor, interrupted osteoclastogenesis stimulation by the glucocorticoids. These results implied that with glucocorticoid administration, ROS and autophagy, as a downstream factor of ROS, played vital roles in osteoclast formation and function. 3-MA administration did not enhance ROS accumulation, so that autophagy had no effect on ROS induced by glucocorticoids. Our investigation demonstrated that glucocorticoids had dose-dependent positive effects on osteoclast formation and function via ROS and autophagy. These results provide support for ROS and autophagy as therapeutic targets in glucocorticoid-related bone loss diseases such as glucocorticoid-induced osteoporosis.
journal_name
Bonejournal_title
Boneauthors
Shi J,Wang L,Zhang H,Jie Q,Li X,Shi Q,Huang Q,Gao B,Han Y,Guo K,Liu J,Yang L,Luo Zdoi
10.1016/j.bone.2015.06.014subject
Has Abstractpub_date
2015-10-01 00:00:00pages
222-32eissn
8756-3282issn
1873-2763pii
S8756-3282(15)00250-1journal_volume
79pub_type
杂志文章相关文献
BONE文献大全abstract：:FK506 is a commonly used immunosuppressant that mediates its action by exclusively interacting with the cytosolic immunophilin, FK506 binding protein 12 (FKBP12). Although FK506-induced acute osteoporosis is now well recognised, its precise mode of action in osteoblasts remains unclear. Therefore, in the present study...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2004.09.021
更新日期：2005-02-01 00:00:00
abstract：:Interleukin-1 (IL-1) has been shown to promote osteoclast (OC) differentiation, in addition to acting as a survival factor for mature osteoclasts. In this study, we investigate the expression of IL-1 during human osteoclast formation, taking advantage of a recently reported in vitro culture system that generates human...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/s8756-3282(99)00176-3
更新日期：1999-09-01 00:00:00
abstract：:Vertebral fracture risk is a heritable complex trait. The aim of this study was to identify genetic susceptibility factors for osteoporotic vertebral fractures applying a genome-wide association study (GWAS) approach. The GWAS discovery was based on the Rotterdam Study, a population-based study of elderly Dutch indivi...
journal_title：Bone
pub_type： 杂志文章
doi：
更新日期：2014-02-01 00:00:00
abstract：:Many osteoporotic fracture patients are candidates for concurrent treatment with bisphosphonates and bioceramic bone graft substitutes. Osteopromotive silica-based bioactive glasses are known to induce accelerated local bone turnover and adjunct antiresorptive agents, such as zoledronic acid, may affect the process. T...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2005.09.016
更新日期：2006-03-01 00:00:00
abstract：:Thanatophoric dysplasia (TD) is a lethal human bone dysplasia characterized by severe dwarfism. It pathogenesis is thought to involve an abnormal ossifying fibrous tissue that disrupts the skeletal growth plate. We employed a combined morphologic, immunohistochemical and biochemical approach to better define the natur...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/8756-3282(88)90027-0
更新日期：1988-01-01 00:00:00
abstract：:Within-bone variation in mineral density could be functional. A heterogeneous mineral-density distribution might serve to maintain habitual amplitudes of bone strain within a non-harmful, i.e., physiological range. Regions of a bone that would be strained the most on the basis of architecture alone might have a higher...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2013.04.026
更新日期：2013-08-01 00:00:00
abstract：:Glucocorticoids act on the osteoblasts to up-regulate the expression of RANKL, which is very important in the etiology of glucocorticoid-induced osteoclast differentiation and bone resorption. The mechanisms of this process are still not completely understood. Recent studies have shown that glucocorticoids mediate ost...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2014.08.004
更新日期：2014-11-01 00:00:00
abstract：:The Sost gene encodes Sclerostin, an inhibitor of Wnt-signaling, generally considered a main response gene to mechanical loading in bone. Several papers describe that unloading leads to upregulation of Sost, which in turn may lead to loss of bone. These studies were based on whole bone homogenates or cortical bone. By...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2013.01.018
更新日期：2013-04-01 00:00:00
abstract：INTRODUCTION:Teriparatide is a bone formation agent that increases bone turnover and mass, resulting in an increase in bone strength and a decrease in fracture risk. METHODS:The primary purpose of this analysis was to evaluate the association between pretreatment bone turnover marker (BTM) concentrations and the absol...
journal_title：Bone
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.bone.2006.02.003
更新日期：2006-08-01 00:00:00
abstract：BACKGROUND:U.S. Army Basic Combat Training (BCT) is a physically-demanding program at the start of military service. Whereas animal studies have shown that increased mechanical loading rapidly alters bone structure, there is limited evidence of changes in bone density and structure in humans exposed to a brief period o...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2018.04.021
更新日期：2018-08-01 00:00:00
abstract：:In 1994, the World Health Organization published diagnostic criteria for osteoporosis. Since then, many new technologies have been developed for the measurement of bone mineral at multiple skeletal sites. The information provided by each assessment will describe the clinical characteristics, fracture risk and epidemio...
journal_title：Bone
pub_type： 杂志文章,评审
doi：10.1016/j.bone.2007.11.001
更新日期：2008-03-01 00:00:00
abstract：:We believe that the assembled data are consistent with the presence of mRNA species and/or proteins in pagetic bone that are recognized by some paramyxovirus antibodies and nucleic acid probes. The evidence presented so far is insufficient to substantiate claims for the "unequivocal" presence of paramyxovirus sequence...
journal_title：Bone
pub_type： 杂志文章,评审
doi：10.1016/s8756-3282(99)00032-0
更新日期：1999-05-01 00:00:00
abstract：:The effects of porosity and pore size; osteonal area, size, and density; mineral content; water content; wet and dry apparent densities; and age on mode I (tensile) and mode II (shear) strain energy release rate were investigated for femoral neck cortical bone from human cadavers aged >/=50 years. The results suggest ...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/S8756-3282(00)00258-1
更新日期：2000-05-01 00:00:00
abstract：:The topological changes in vertebral cancellous bone were estimated in vertebrae from calcium-restricted ovariectomized Sinclair S-1 minipigs, a recently described animal model of cancellous osteopenia. Connectivity was estimated using unbiased stereological principles in disector pairs of sections from the first lumb...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/8756-3282(95)00116-u
更新日期：1995-06-01 00:00:00
abstract：:Various substitution mutations in ALK2, a transmembrane serine/threonine kinase receptor for bone morphogenetic proteins (BMPs), have been identified in patients with genetic disorders such as fibrodysplasia ossificans progressiva (FOP), diffuse intrinsic pontine glioma (DIPG) and heart defects. In this study, we char...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2018.03.015
更新日期：2018-06-01 00:00:00
abstract：:Interstitial bone fluid flow (IBFF) is suggested as a communication medium that bridges external physical signals and internal cellular activities in the bone, which thus regulates bone remodeling. Intramedullary pressure (ImP) is one main regulatory factor of IBFF and bone adaptation related mechanotransduction. Our ...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2014.05.018
更新日期：2014-09-01 00:00:00
abstract：:This review begins with an introduction to the malignant bone tumor, osteosarcoma [OS] and then moves to a discussion of the commonly used vectors for gene transfer. We first briefly highlight non-viral vectors including polymeric and liposomal delivery systems but concentrate predominantly on the 5 leading viral vect...
journal_title：Bone
pub_type： 杂志文章,评审
doi：10.1016/j.bone.2006.10.017
更新日期：2007-04-01 00:00:00
abstract：:Recently the third generation aromatase inhibitors have proved their efficacy and tolerability compared with tamoxifen in the adjuvant treatment of women with hormone responsive early breast cancer. However, there is some concern about the possible negative impact of these drugs on bone. The aim of the study was to ev...
journal_title：Bone
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.bone.2006.06.027
更新日期：2007-01-01 00:00:00
abstract：:This cohort study of postmenopausal women in the United Kingdom aged ≥50years determined the incremental cost of health care and clinical outcomes in the 12months following incident, selected fractures (non-vertebral non-hip [NVNHF], vertebral [VF] and multiple [MF]). Incremental costs and outcomes of the fracture coh...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2012.05.020
更新日期：2012-09-01 00:00:00
abstract：:Macrometastases in bone are preceded by bone marrow invasion of disseminated tumor cells. This study combined functional imaging parameters from FDG-PET/CT and MRI in a rat model of breast cancer bone metastases to a Model-averaged Neural Network (avNNet) for the detection of early metastatic disease and prediction of...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2018.11.008
更新日期：2019-03-01 00:00:00
abstract：:Paralysis of the masticatory muscles using botulinum toxin (BTX) is a common treatment for cosmetic reduction of the masseters as well as for conditions involving muscle spasm and pain. The effects of this treatment on mastication have not been evaluated, and claims that the treatment unloads the jaw joint and mandibl...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2011.11.015
更新日期：2012-03-01 00:00:00
abstract：:In order to assess the ability of six potential bone graft substitutes to support the growth of human osteoblasts, these cells were grown in culture and then plated onto fragments of the six materials and cultured for a further period of 15 days. Tests to confirm the osteoblastic phenotype of the cells included spectr...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/8756-3282(93)90089-s
更新日期：1993-07-01 00:00:00
abstract：:The signals that allow bone to adapt to its mechanical environment most likely involve strain-mediated fluid flow through the canalicular channels. Fluid can only be moved through bone by cyclic loading, and the shear stresses generated on bone cells are proportional to the rate of loading. The proportional relation b...
journal_title：Bone
pub_type： 杂志文章,评审
doi：10.1016/s8756-3282(02)00707-x
更新日期：2002-05-01 00:00:00
abstract：:The cartilage- and bone-forming properties of the periosteum have long since been recognized. As one of the major sources of skeletal progenitor cells, the periosteum plays a crucial role not only in bone development and growth, but also during bone fracture healing. Aided by the continuous expansion of tools and tech...
journal_title：Bone
pub_type： 杂志文章,评审
doi：10.1016/j.bone.2014.08.007
更新日期：2015-01-01 00:00:00
abstract：OBJECTIVES:The present study was motivated by the fact that bone regeneration in the compromised vascular microenvironment of T2DM is challenging and the factors that determine the adverse bone regeneration outcomes are poorly understood. For this purpose the effect of T2DM on osteogenic and angiogenic healing potentia...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2018.04.009
更新日期：2018-07-01 00:00:00
abstract：:The aim of this study was to estimate the burden of childhood rickets-like bone deformity in a rural region of West Africa where rickets has been reported in association with a low calcium intake. A population-based survey of children aged 0.5-17.9 years living in the province of West Kiang, The Gambia was conducted i...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2015.04.011
更新日期：2015-08-01 00:00:00
abstract：BACKGROUND:The present study aimed to investigate the association between type 2 diabetes mellitus (T2DM) and hip fractures using a large-scale nationwide population-based cohort that is representative of the Republic of Korea. We determined the risks of hip fractures in individuals with prediabetes and T2DM with diffe...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2020.115691
更新日期：2021-01-01 00:00:00
abstract：:This multicenter study assessed the safety and efficacy of teriparatide 20 microg/day in Japanese men and women with osteoporosis at high risk of fracture during a 12-month, randomized, double-blind, placebo-controlled treatment period followed by second and third treatment periods (to 18 and 24 months, respectively,)...
journal_title：Bone
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.bone.2010.05.022
更新日期：2010-09-01 00:00:00
abstract：:It was the aim of this retrospective analysis to examine the influence of low-dose monophasic oral contraceptives (OCs) on bone mineral density (BMD) of the femoral neck and of the spine in young female endurance athletes. Data on training intensity, dietary intake, menarche, menstrual cycle disorders, years of OC use...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2004.05.017
更新日期：2004-10-01 00:00:00
abstract：:Mechanical signal transduction in bone tissue begins with load-induced activation of several cellular pathways in the osteocyte population. A key pathway that participates in mechanotransduction is Wnt/Lrp5 signaling. A putative downstream mediator of activated Lrp5 is the nucleocytoplasmic shuttling protein β-catenin...
journal_title：Bone
pub_type： 杂志文章
doi：10.1016/j.bone.2016.04.028
更新日期：2016-07-01 00:00:00
