当前位置： SCI文献检索 > BONE期刊下所有文献 > MicroRNA-17/20a inhibits glucocorticoid-induced osteoclast differentiation and function through targeting RANKL expression in osteoblast cells.

MicroRNA-17/20a inhibits glucocorticoid-induced osteoclast differentiation and function through targeting RANKL expression in osteoblast cells.

Abstract：

:Glucocorticoids act on the osteoblasts to up-regulate the expression of RANKL, which is very important in the etiology of glucocorticoid-induced osteoclast differentiation and bone resorption. The mechanisms of this process are still not completely understood. Recent studies have shown that glucocorticoids mediate osteoblast function by decreasing the expression of microRNA-17-92a cluster. Coincidentally, we found that the microRNA-17/20a (microRNA-17, microRNA-20a) seed sequences were also complementary to a sequence conserved in the 3'- untranslated region of RANKL mRNA. Therefore, we hypothesized that glucocorticoids might promote osteoblast-derived RANKL expression by down-regulating microRNA-17/20a, which favors differentiation and function of the osteoclasts. In the present study, Western blot analysis showed that microRNA-17/20a markedly lowered the levels of RANKL protein and attenuated dexamethasone-induced RANKL expression in the osteoblasts. The post-transcriptional repression of RANKL by microRNA-17/20a was further confirmed by the luciferase reporter assay. Furthermore, we found that dexamethasone-induced osteoclast differentiation and function were significantly attenuated in co-culture with osteoblast over-expressed microRNA-17/20a and osteoclast progenitors. These results showed that microRNA-17/20a may play a significant role in glucocorticoid-induced osteoclast differentiation and function by targeting the RANKL expression in osteoblast cells.

journal_name

Bone

journal_title

Bone

authors

Shi C,Qi J,Huang P,Jiang M,Zhou Q,Zhou H,Kang H,Qian N,Yang Q,Guo L,Deng L

doi

10.1016/j.bone.2014.08.004

subject

Has Abstract

pub_date

2014-11-01 00:00:00

pages

67-75

eissn

8756-3282

issn

1873-2763

pii

S8756-3282(14)00305-6

journal_volume

68

pub_type

杂志文章

相关文献

BONE文献大全
  • Pregnancy outcome following in utero exposure to bisphosphonates.

    abstract：BACKGROUND AND AIM:The safety of bisphosphonates in human pregnancy has not been well established. To characterize pregnancy outcome in women receiving bisphosphonates, we conducted a multi-centre, prospective cohort study with a comparison group. METHODS:Patients were recruited through 3 teratogen information centres...

    journal_title：Bone

    pub_type： 临床试验,杂志文章,多中心研究

    doi：10.1016/j.bone.2008.11.001

    authors： Levy S,Fayez I,Taguchi N,Han JY,Aiello J,Matsui D,Moretti M,Koren G,Ito S

    更新日期：2009-03-01 00:00:00

  • The beneficial effect of icaritin on osteoporotic bone is dependent on the treatment initiation timing in adult ovariectomized rats.

    abstract：BACKGROUND:Epimedium-derived flavonoids (EFs) have a potential to treat established osteoporosis in postmenopausal women. However, one of the main disadvantages of the compound is the high volume and dosage during long-term administration period. Meanwhile, the beneficial effect of EFs on osteoporotic bone depends grea...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2013.02.012

    authors： Peng S,Zhang G,Zhang BT,Guo B,He Y,Bakker AJ,Pan X,Zhen W,Hung L,Qin L,Leung WN

    更新日期：2013-07-01 00:00:00

  • 1,25(OH)2D deficiency induces temporomandibular joint osteoarthritis via secretion of senescence-associated inflammatory cytokines.

    abstract：:1,25-Dihydroxyvitamin D [1,25(OH)(2)D] insufficiency appears to be associated with several age-related diseases. Insufficient levels of serum 25-hydroxyvitamin D has been shown to lead to the progression of osteoarthritis (OA) while underlying biological mechanisms remain largely unknown. In this study, we sought to d...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2013.04.015

    authors： Shen M,Luo Y,Niu Y,Chen L,Yuan X,Goltzman D,Chen N,Miao D

    更新日期：2013-08-01 00:00:00

  • Changes in marrow adipose tissue in relation to changes in bone parameters following estradiol replacement in adolescent and young adult females with functional hypothalamic amenorrhea.

    abstract：CONTEXT:Low energy availability causes disruption of hypothalamic gonadotropin-releasing hormone secretion leading to functional hypothalamic amenorrhea (FHA) and hypoestrogenism, which in turn contributes to decreased bone mineral density (BMD) and increased bone marrow adipose tissue (MAT). Transdermal estradiol admi...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2021.115841

    authors： Singhal V,Karzar NH,Bose A,Buckless C,Ackerman KE,Bredella MA,Klibanski A,Misra M

    更新日期：2021-01-06 00:00:00

  • Trabecular bone loss after administration of the second-generation antipsychotic risperidone is independent of weight gain.

    abstract：:Second generation antipsychotics (SGAs) have been linked to metabolic and bone disorders in clinical studies, but the mechanisms of these side effects remain unclear. Additionally, no studies have examined whether SGAs cause bone loss in mice. Using in vivo and in vitro modeling we examined the effects of risperidone,...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2011.08.005

    authors： Motyl KJ,Dick-de-Paula I,Maloney AE,Lotinun S,Bornstein S,de Paula FJ,Baron R,Houseknecht KL,Rosen CJ

    更新日期：2012-02-01 00:00:00

  • How B cells influence bone biology in health and disease.

    abstract：:It is now well established that important regulatory interactions occur between the cells in the hematopoietic, immune and skeletal systems (osteoimmunology). B lymphocytes (B cells) are responsible for the generation and production of antibodies or immunoglobulins in the body. Together with T cells these lymphocytes ...

    journal_title：Bone

    pub_type： 杂志文章,评审

    doi：10.1016/j.bone.2010.06.011

    authors： Horowitz MC,Fretz JA,Lorenzo JA

    更新日期：2010-09-01 00:00:00

  • Absence of an osteopetrosis phenotype in IKBKG (NEMO) mutation-positive women: A case-control study.

    abstract：BACKGROUND:NF-κB essential modulator (NEMO), encoded by IKBKG, is necessary for activation of the ubiquitous transcription factor nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). Animal studies suggest NEMO is required for NF-κB mediated bone homeostasis, but this has not been thoroughly studied ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2019.01.014

    authors： Frost M,Tencerova M,Andreasen CM,Andersen TL,Ejersted C,Svaneby D,Qui W,Kassem M,Zarei A,McAlister WH,Veis DJ,Whyte MP,Frederiksen AL

    更新日期：2019-04-01 00:00:00

  • miRNAs in osteoclast biology.

    abstract：:MicroRNAs (miRNAs) are a class of short RNA molecules that mediate the regulation of gene activity through interactions with target mRNAs and subsequent silencing of gene expression. It has become increasingly clear the miRNAs regulate many diverse aspects of bone biology, including bone formation and bone resorption ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2020.115757

    authors： Weivoda MM,Lee SK,Monroe DG

    更新日期：2021-02-01 00:00:00

  • Novel SLC34A3 mutation causing hereditary hypophosphataemic rickets with hypercalciuria in a Gambian family.

    abstract：:Three siblings, aged 12, 4 and 2 years, presented at a Gambian clinic with bone deformities. Radiographs of knees and wrists confirmed the presence of florid rickets. The family (including 2 unaffected siblings and the mother) were investigated for hereditary rickets. The three affected siblings had biochemical featur...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2012.12.003

    authors： Braithwaite V,Pettifor JM,Prentice A

    更新日期：2013-03-01 00:00:00

  • Changes in Dkk-1, sclerostin, and RANKL serum levels following discontinuation of long-term denosumab treatment in postmenopausal women.

    abstract：PURPOSE:The positive effects of denosumab (DMAb) on bone mineral density (BMD) are quickly reversible after its discontinuation. We investigated whether this rebound was associated with dysregulation of the Wnt canonical pathway and/or by the increase in the receptor-activator of nuclear factor-kappa B ligand (RANKL) s...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2019.03.019

    authors： Fassio A,Adami G,Benini C,Vantaggiato E,Saag KG,Giollo A,Lippolis I,Viapiana O,Idolazzi L,Orsolini G,Rossini M,Gatti D

    更新日期：2019-06-01 00:00:00

  • Subregional areal bone mineral density (aBMD) is a better predictor of heterogeneity in trabecular microstructure of vertebrae in young and aged women than subregional trabecular bone score (TBS).

    abstract：BACKGROUND:Currently, bone densitometry fails to identify nearly half of those elderly patients at immediate fracture risk. To improve clinical assessment of vertebral fracture risk, we aimed to determine how the DXA-based 2D parameter Trabecular Bone Score (TBS) relates to subregional variability in 3D trabecular micr...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2019.02.014

    authors： Vom Scheidt A,Grisolia Seifert EF,Pokrant C,Püschel K,Amling M,Busse B,Milovanovic P

    更新日期：2019-05-01 00:00:00

  • Histomorphometric changes in the trabecular structure of a selected stress region in the femur in patients with osteoarthritis and fracture of the femoral neck.

    abstract：:Histomorphometric changes in the trabecular structure of the principal compressive stress region in the femur in patients with osteoarthritis and fracture of the femoral neck are described. Femoral heads were obtained from patients who had surgery for the treatment of femoral neck fracture or total hip replacement for...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/8756-3282(85)90044-4

    authors： Fazzalari NL,Darracott J,Vernon-Roberts B

    更新日期：1985-01-01 00:00:00

  • Evidence for secular change in Paget's disease.

    abstract：:Death certification data has shown that death rates due to Paget's disease of bone and osteosarcoma in older people (assumed to be attributable to Paget's) declined in the latter part of the 19th and in the early 20th century, suggesting that there may be a secular trend toward less severe disease. We have reviewed a ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(96)00310-9

    authors： Cundy T,McAnulty K,Wattie D,Gamble G,Rutland M,Ibbertson HK

    更新日期：1997-01-01 00:00:00

  • Impaired bone healing pattern in mice with ovariectomy-induced osteoporosis: A drill-hole defect model.

    abstract：OBJECTIVE:To establish a drill-hole defect model in osteoporotic mouse femur by comparing temporal cortical bone healing pattern between OVX-induced osteoporotic bone and sham-operated bone. METHODS:3-month-old female C57BL/6 mice were randomly divided into an ovariectomy group (OVX) and a sham-operated group (Sham). ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2011.03.720

    authors： He YX,Zhang G,Pan XH,Liu Z,Zheng LZ,Chan CW,Lee KM,Cao YP,Li G,Wei L,Hung LK,Leung KS,Qin L

    更新日期：2011-06-01 00:00:00

  • Effect of inducible bone morphogenetic protein 2 expression on the osteogenic differentiation of dental pulp stem cells in vitro.

    abstract：:Bone morphogenetic protein 2 (BMP-2) is a member of the transforming growth factor-β superfamily, it is known to be a factor involved in skeletal development and capable of inducing in vitro osteogenic differentiation of mesenchymal stem cells (MSCs). Dental pulp stem cells (DPSCs) isolated from extracted third molar ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2019.115214

    authors： Tóth F,Gáll JM,Tőzsér J,Hegedűs C

    更新日期：2020-03-01 00:00:00

  • Expression of amelogenin in odontoblasts.

    abstract：:Amelogenin is the major enamel protein produced by ameloblasts. Its expression has been shown to be down-regulated in ameloblasts of vitamin-D-deficient (-D) rats. The potential expression and localization of amelogenin in odontoblasts and its regulation by vitamin D were investigated in this study. RT-PCR and semi-qu...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(02)00978-x

    authors： Papagerakis P,MacDougall M,Hotton D,Bailleul-Forestier I,Oboeuf M,Berdal A

    更新日期：2003-03-01 00:00:00

  • Aging of mouse intervertebral disc and association with back pain.

    abstract：:With the increased burden of low back pain (LBP) in our globally aging population there is a need to develop preclinical models of LBP that capture clinically relevant features of physiological aging, degeneration, and disability. Here we assess the validity of using a mouse model system for age-related LBP by charact...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2019.03.037

    authors： Vincent K,Mohanty S,Pinelli R,Bonavita R,Pricop P,Albert TJ,Dahia CL

    更新日期：2019-06-01 00:00:00

  • Fracture risk reduction during treatment with teriparatide is independent of pretreatment bone turnover.

    abstract：INTRODUCTION:Teriparatide is a bone formation agent that increases bone turnover and mass, resulting in an increase in bone strength and a decrease in fracture risk. METHODS:The primary purpose of this analysis was to evaluate the association between pretreatment bone turnover marker (BTM) concentrations and the absol...

    journal_title：Bone

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/j.bone.2006.02.003

    authors： Delmas PD,Licata AA,Reginster JY,Crans GG,Chen P,Misurski DA,Wagman RB,Mitlak BH

    更新日期：2006-08-01 00:00:00

  • A homozygous intronic branch-point deletion in the ALPL gene causes infantile hypophosphatasia.

    abstract：:Hypophosphatasia (HPP) is a multi-systemic inborn disease with an extraordinary spectrum of severity, ranging from the absence of mineralization to high lethality and it involves different organs including bone, muscle, kidney, lung, gastrointestinal tract and the nervous system. The disease is characterized by low le...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2016.10.022

    authors： Mentrup B,Girschick H,Jakob F,Hofmann C

    更新日期：2017-01-01 00:00:00

  • The fabrication of nano-hydroxyapatite on PLGA and PLGA/collagen nanofibrous composite scaffolds and their effects in osteoblastic behavior for bone tissue engineering.

    abstract：:Bone is a nanocomposite consisting of two main components, nano-hydroxyapatite (n-HA) and Type I collagen (Col). The aim is to exploit the nano-scale functional and material characteristics of natural bone in order to modulate cellular functions for optimal bone repair in bone graft systems. Here, we present an effect...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2009.03.674

    authors： Ngiam M,Liao S,Patil AJ,Cheng Z,Chan CK,Ramakrishna S

    更新日期：2009-07-01 00:00:00

  • 3D hierarchical geometric modeling and multiscale FE analysis as a base for individualized medical diagnosis of bone structure.

    abstract：:This paper describes a new alternative for individualized mechanical analysis of bone trabecular structure. This new method closes the gap between the classic homogenization approach that is applied to macro-scale models and the modern micro-finite element method that is applied directly to micro-scale high-resolution...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2010.12.022

    authors： Podshivalov L,Fischer A,Bar-Yoseph PZ

    更新日期：2011-04-01 00:00:00

  • Exercise prevents high fat diet-induced bone loss, marrow adiposity and dysbiosis in male mice.

    abstract：:High fat diets can have detrimental effects on the skeleton as well as cause intestinal dysbiosis. Exercise prevents high fat (HF) diet-induced obesity and also improves bone density and prevents the intestinal dysbiosis that promotes energy storage. Previous studies indicate a link between intestinal microbial balanc...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2018.03.024

    authors： McCabe LR,Irwin R,Tekalur A,Evans C,Schepper JD,Parameswaran N,Ciancio M

    更新日期：2019-01-01 00:00:00

  • Altered mRNA level of matrix metalloproteinase-13 in MH7A synovial cells under mechanical loading and unloading.

    abstract：:In an effort to elucidate the interplay between mechanical load and proteolytic gene expression in arthritic tissue degradation, we investigated cellular morphology and mRNA levels of matrix metalloproteinase-13 (MMP-13) genes under mechanical stress in human MH7A synovial cells. The cells were isolated from the knee ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(00)00459-2

    authors： Sun HB,Yokota H

    更新日期：2001-04-01 00:00:00

  • Use of an alpha-smooth muscle actin GFP reporter to identify an osteoprogenitor population.

    abstract：:Identification of a reliable marker of skeletal precursor cells within calcified and soft tissues remains a major challenge for the field. To address this, we used a transgenic model in which osteoblasts can be eliminated by pharmacological treatment. Following osteoblast ablation a dramatic increase in a population o...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2008.04.023

    authors： Kalajzic Z,Li H,Wang LP,Jiang X,Lamothe K,Adams DJ,Aguila HL,Rowe DW,Kalajzic I

    更新日期：2008-09-01 00:00:00

  • Expression of bone microsomal casein kinase II, bone sialoprotein, and osteopontin during the repair of calvarial defects.

    abstract：:The temporal expression of bone microsomal casein kinase II, osteopontin, bone sialoprotein, alkaline phosphatase, and the accumulation of a solid calcium-inorganic orthophosphate mineral phase, have been charted from day 2 to day 21 during the repair of calvarial defects in rats induced by the implantation of decalci...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(98)00057-x

    authors： Wang J,Glimcher MJ,Mah J,Zhou HY,Salih E

    更新日期：1998-06-01 00:00:00

  • Transforming growth factor beta 1 stimulates bone formation and resorption in an in-vivo model in rabbits.

    abstract：:The influence of TGF beta 1 on bone was studied in a titanium device implanted into the tibia of rabbits. TGF beta 1 was infused via an Alzet osmotic pump calibrated to deliver at a rated of 200ng daily for 2 weeks before replacement. A hollow channel is incorporated into the device into which tissue can grow, and the...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/8756-3282(95)00324-7

    authors： Zhou H,Choong PC,Chou ST,Kartsogiannis V,Martin TJ,Ng KW

    更新日期：1995-10-01 00:00:00

  • Osteopontin gene expression and alkaline phosphatase activity in avian tibial dyschondroplasia.

    abstract：:Osteopontin (OPN) gene expression and alkaline phosphatase activity were evaluated in the epiphyseal growth plates of normal chickens and in diet-induced tibial dyschdroplasia (TD)-afflicted chickens. In the normal growth plate, OPN gene was expressed by a) cells of the subperichondrial zone surrounding the articular ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/8756-3282(94)00048-5

    authors： Knopov V,Leach RM,Barak-Shalom T,Hurwitz S,Pines M

    更新日期：1995-04-01 00:00:00

  • Influence of food and antacid administration on fluoride bioavailability from enteric-coated sodium fluoride tablets.

    abstract：:The relative bioavailability of enteric-coated sodium fluoride (NaF) tablets (10 mg F-) has been assessed following administration with a standard calcium-rich breakfast or calcium-poor lunch, and 2 h before or simultaneously with antacid administration (2.4 g aluminum-magnesium hydroxide), versus intake on an empty s...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/8756-3282(89)90070-7

    authors： Arnold P,Wermeille M,Chapuy MC,Biollaz J,Grandjean EM,Schelling JL,Meunier PJ

    更新日期：1989-01-01 00:00:00

  • Mechanisms of palmitate-induced lipotoxicity in osteocytes.

    abstract：BACKGROUND:Lipotoxicity is defined as cellular toxicity observed in the presence of an abnormal accumulation of fat and adipocyte-derived factors in non-fat tissues. Palmitic acid (PA), an abundant fatty acid in the bone marrow and particularly in osteoporotic bones, affects osteoblastogenesis and osteoblast function, ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2019.06.016

    authors： Al Saedi A,Bermeo S,Plotkin L,Myers DE,Duque G

    更新日期：2019-10-01 00:00:00

  • A nonhuman primate model of age-related bone loss: a longitudinal study in male and premenopausal female rhesus monkeys.

    abstract：:Aging is associated with gradual bone loss in men and premenopausal women, with an accelerated rate of loss after menopause in women. Although many studies have investigated bone loss due to surgically induced estrogen depletion, little is known regarding normal age-related changes in bone mass in animal models. We us...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(00)00452-x

    authors： Black A,Tilmont EM,Handy AM,Scott WW,Shapses SA,Ingram DK,Roth GS,Lane MA

    更新日期：2001-03-01 00:00:00