当前位置： SCI文献检索 > BONE期刊下所有文献 > Mechanisms of palmitate-induced lipotoxicity in osteocytes.

Mechanisms of palmitate-induced lipotoxicity in osteocytes.

Abstract：

BACKGROUND:Lipotoxicity is defined as cellular toxicity observed in the presence of an abnormal accumulation of fat and adipocyte-derived factors in non-fat tissues. Palmitic acid (PA), an abundant fatty acid in the bone marrow and particularly in osteoporotic bones, affects osteoblastogenesis and osteoblast function, decreasing their survival through induction of apoptosis and dysfunctional autophagy. In this study, we hypothesized that PA also has a lipotoxic effect on osteocytes in vitro. METHODS:Initially, we tested the effect of PA on osteocyte-derived factors DKK1, sclerostin and RANKL. Then, we tested whether PA affects survival and causes apoptosis in osteocytes. Subsequently, we investigated the effect of PA on autophagy by detecting the membrane component LC3-II (Western blot) and staining it and lysosomes with Lysotracker Red dye. RESULTS:PA decreases RANKL, DKK1 and sclerostin expression in osteocytes. In addition, we found that PA induces apoptosis and reduces osteocyte survival. PA also caused autophagy failure identified by a significant increase in LC3-II and a reduced number of autophagosomes/lysosomes in the cytoplasm. CONCLUSION:In addition to the effects of PA on RANKL, DKK1 and sclerostin expression, which could have significant deleterious impact on bone cell coupling and bone turnover, PA also induced apoptosis and reduced autophagy in osteocytes. Considering that apoptosis and cell dysfunction are two common changes occurring in the osteocytes of osteoporotic bone, our findings suggest that PA could play a role in the pathogenesis of the disease. Suppression of these effects could bring new potential targets for therapeutic interventions in the future.

journal_name

Bone

journal_title

Bone

authors

Al Saedi A,Bermeo S,Plotkin L,Myers DE,Duque G

doi

10.1016/j.bone.2019.06.016

subject

Has Abstract

pub_date

2019-10-01 00:00:00

pages

353-359

eissn

8756-3282

issn

1873-2763

pii

S8756-3282(19)30248-0

journal_volume

127

pub_type

杂志文章

相关文献

BONE文献大全
  • PINP: a serum biomarker of bone formation in the rat.

    abstract：:Serum PINP has emerged as a reliable marker of bone turnover in humans and is routinely used to monitor bone formation. However, the effects of PTH (1-34) on bone turnover have not been evaluated following short-term treatment. We present data demonstrating that PINP is an early serum biomarker in the rat for assessin...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2006.11.027

    authors： Hale LV,Galvin RJ,Risteli J,Ma YL,Harvey AK,Yang X,Cain RL,Zeng Q,Frolik CA,Sato M,Schmidt AL,Geiser AG

    更新日期：2007-04-01 00:00:00

  • Biological apatite (BAp) crystallographic orientation and texture as a new index for assessing the microstructure and function of bone regenerated by tissue engineering.

    abstract：:Recently, there have been remarkable advances in medical techniques for regenerating bone defects. To determine the degree of bone regeneration, it is essential to develop a new method that can analyze microstructure and related mechanical function. Here, quantitative analysis of the orientation distribution of biolog...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2012.07.003

    authors： Nakano T,Kaibara K,Ishimoto T,Tabata Y,Umakoshi Y

    更新日期：2012-10-01 00:00:00

  • Impaired bone healing pattern in mice with ovariectomy-induced osteoporosis: A drill-hole defect model.

    abstract：OBJECTIVE:To establish a drill-hole defect model in osteoporotic mouse femur by comparing temporal cortical bone healing pattern between OVX-induced osteoporotic bone and sham-operated bone. METHODS:3-month-old female C57BL/6 mice were randomly divided into an ovariectomy group (OVX) and a sham-operated group (Sham). ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2011.03.720

    authors： He YX,Zhang G,Pan XH,Liu Z,Zheng LZ,Chan CW,Lee KM,Cao YP,Li G,Wei L,Hung LK,Leung KS,Qin L

    更新日期：2011-06-01 00:00:00

  • Monitoring individual morphological changes over time in ovariectomized rats by in vivo micro-computed tomography.

    abstract：:The ovariectomized (OVX) rat is a well established model for osteoporosis research. The recent development of in vivo micro-computed tomography (micro-CT) provides new possibilities to monitor individual bone changes over time. The purpose of this study was to establish the normal time course of bone loss in the OVX r...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2006.04.017

    authors： Boyd SK,Davison P,Müller R,Gasser JA

    更新日期：2006-10-01 00:00:00

  • Mitf and Tfe3: members of a b-HLH-ZIP transcription factor family essential for osteoclast development and function.

    abstract：:The Microphthalmia-associated transcription factor (Mitf) is required for the proper development of several cell lineages including osteoclasts, melanocytes, retinal pigment epithelial cells, mast cells and natural killer cells. Mutations in Mitf in multiple organisms result in osteopetrosis due to defective osteoclas...

    journal_title：Bone

    pub_type： 杂志文章,评审

    doi：10.1016/j.bone.2003.08.014

    authors： Hershey CL,Fisher DE

    更新日期：2004-04-01 00:00:00

  • Mouse polycomb group gene Cbx2 promotes osteoblastic but suppresses adipogenic differentiation in postnatal long bones.

    abstract：:A set of key developmental genes is essential for skeletal growth from multipotent progenitor cells at weaning. Polycomb group proteins, which regulate such genes contributes to the cell lineage commitment and subsequent differentiation via epigenetic chromatin modification and remodeling. However, it is unclear which...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2018.10.021

    authors： Katoh-Fukui Y,Baba T,Sato T,Otake H,Nagakui-Noguchi Y,Shindo M,Suyama M,Ohkawa Y,Tsumura H,Morohashi KI,Fukami M

    更新日期：2019-03-01 00:00:00

  • 1Alpha,25-dihydroxy-2beta(3-hydroxypropoxy)vitamin D3 (ED-71) suppressed callus remodeling but did not interfere with fracture healing in rat femora.

    abstract：INTRODUCTION:Because osteoporotic patients are prone to fractures, it must be considered whether or not patients undergoing drug therapies should discontinue treatment after sustaining a non-vertebral fracture. This study has tested the effect of novel active vitamin D3 analog, 1alpha,25-dihydroxy-2beta(3-hydroxypropox...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2006.07.023

    authors： Cao Y,Mori S,Mashiba T,Kaji Y,Manabe T,Iwata K,Miyamoto K,Komatsubara S,Yamamoto T

    更新日期：2007-01-01 00:00:00

  • Roles of PKC, PI3K and JNK in multiple transduction of CCN2/CTGF signals in chondrocytes.

    abstract：:CCN2/connective tissue growth factor (CCN2/CTGF) is known to promote both the proliferation and differentiation of chondrocytes, which actions are mediated by ERK and p38 MAPK, respectively. In this study, we first re-evaluated the involvement of multiple MAPKs therein and found that JNK also mediated such CCN2 signal...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2005.11.016

    authors： Yosimichi G,Kubota S,Nishida T,Kondo S,Yanagita T,Nakao K,Takano-Yamamoto T,Takigawa M

    更新日期：2006-06-01 00:00:00

  • Computational finite element bone mechanics accurately predicts mechanical competence in the human radius of an elderly population.

    abstract：:High-resolution peripheral quantitative computed tomography (HR-pQCT) is clinically available today and provides a non-invasive measure of 3D bone geometry and micro-architecture with unprecedented detail. In combination with microarchitectural finite element (μFE) models it can be used to determine bone strength usin...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2011.02.022

    authors： Mueller TL,Christen D,Sandercott S,Boyd SK,van Rietbergen B,Eckstein F,Lochmüller EM,Müller R,van Lenthe GH

    更新日期：2011-06-01 00:00:00

  • Vitamin D sterols increase FGF23 expression by stimulating osteoblast and osteocyte maturation in CKD bone.

    abstract：:Impaired osteoblast and osteocyte maturation contribute to mineralization defects and excess FGF23 expression in CKD bone. Vitamin D sterols decrease osteoid accumulation and increase FGF23 expression; these agents also increase osteoblast maturation in vitro but a link between changes in bone cell maturation, bone mi...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2019.07.026

    authors： Pereira RC,Salusky IB,Bowen RE,Freymiller EG,Wesseling-Perry K

    更新日期：2019-10-01 00:00:00

  • Eldecalcitol, a second-generation vitamin D analog, drives bone minimodeling and reduces osteoclastic number in trabecular bone of ovariectomized rats.

    abstract：:To elucidate the histological events that follow administration of eldecalcitol, a second-generation of vitamin D analog currently awaiting approval as a drug for treatment of osteoporosis, we employed the ovariectomy (OVX) rat model. OVX rats received vehicle or 30ng/kg of eldecalcitol, and sham-operated animals rece...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2011.05.022

    authors： de Freitas PH,Hasegawa T,Takeda S,Sasaki M,Tabata C,Oda K,Li M,Saito H,Amizuka N

    更新日期：2011-09-01 00:00:00

  • Breaking crown dentine in whole teeth: 3D observations of prevalent fracture patterns following overload.

    abstract：:Teeth with intact crowns rarely split or fracture, despite decades of cyclic loading and occasional unexpected overload. This is largely attributed to the presence of dentine, since cracking and fracture of enamel have been frequently reported. Dentine is similar to bone, comprising mineralised collagen fibres as a ma...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2019.115178

    authors： Fleck C,Burke M,Ganzosch G,Müller C,Currey JD,Zaslansky P

    更新日期：2020-03-01 00:00:00

  • LMP1 regulates periodontal ligament progenitor cell proliferation and differentiation.

    abstract：:LMP1 is an intracellular scaffold protein that contains a PDZ domain and three LIM domains. LMP1 has multiple functions including regulating mesenchymal stem cell (MSC) osteogenesis. Gene delivery of LMP1 induces bone formation in vivo in heterotopic and orthotopic sites. However, little is known about the physiologic...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2010.03.013

    authors： Lin Z,Navarro VP,Kempeinen KM,Franco LM,Jin Q,Sugai JV,Giannobile WV

    更新日期：2010-07-01 00:00:00

  • The 24-hour serum profiles of bone markers in healthy older men and women.

    abstract：:The process of bone turnover displays variations over 24 h, with C-terminal cross-linked telopeptide of type 1 collagen (CTX) and osteocalcin exhibiting a nadir in the afternoon and a peak in the night. In contrast, N-terminal propeptide of type 1 procollagen (P1NP) did not display an apparent 24-hour rhythm. Other em...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2018.10.002

    authors： van der Spoel E,Oei N,Cachucho R,Roelfsema F,Berbée JFP,Blauw GJ,Pijl H,Appelman-Dijkstra NM,van Heemst D

    更新日期：2019-03-01 00:00:00

  • Effects of long-term vitamin K(1) (phylloquinone) or vitamin K(2) (menaquinone-4) supplementation on body composition and serum parameters in rats.

    abstract：:Vitamin K is a cofactor for γ-glutamyl carboxylase, which is an essential enzyme for the γ-carboxylation of vitamin K-dependent proteins such as osteocalcin and matrix Gla protein. Although it has been suggested that vitamin K plays an important role in the improvement of bone metabolism, the relationship between diet...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2011.01.020

    authors： Sogabe N,Maruyama R,Baba O,Hosoi T,Goseki-Sone M

    更新日期：2011-05-01 00:00:00

  • Formoterol and isoproterenol induce c-fos gene expression in osteoblast-like cells by activating beta2-adrenergic receptors.

    abstract：:Formoterol, a beta2-adrenergic agonist has been shown in ovariectomized rat models to have anabolic effects on bone. However, those studies did not determine whether the effect of formoterol was by a direct action on bone cells themselves or indirectly via anabolic action on muscle. To address the question of whether ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(98)00026-x

    authors： Kellenberger S,Muller K,Richener H,Bilbe G

    更新日期：1998-05-01 00:00:00

  • Stanniocalcin 2 is associated with ectopic calcification in α-klotho mutant mice and inhibits hyperphosphatemia-induced calcification in aortic vascular smooth muscle cells.

    abstract：:Ectopic calcification of soft tissues can have severe clinical consequences especially when localized to vital organs such as heart, arteries and kidneys. Mammalian stanniocalcin (STC) 1 and 2 are glycoprotein hormones identified as calcium/phosphate-regulating hormones. The mRNA of STCs is upregulated in the kidney o...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2012.01.006

    authors： Takei Y,Yamamoto H,Sato T,Otani A,Kozai M,Masuda M,Taketani Y,Muto-Sato K,Lanske B,Takeda E

    更新日期：2012-04-01 00:00:00

  • Does serum cholesterol contribute to vertebral bone loss in postmenopausal women?

    abstract：:Recent in vitro and animal studies suggest that cholesterol and its metabolites inhibit the functional activity of osteoblasts and thereby induce reduced bone mineralization. However, scant information is available on the clinical implication of these findings with special regard to postmenopausal bone loss. Therefore...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(02)00918-3

    authors： Tankó LB,Bagger YZ,Nielsen SB,Christiansen C

    更新日期：2003-01-01 00:00:00

  • NHERF1 regulation of PTH-dependent bimodal Pi transport in osteoblasts.

    abstract：:Control of systemic inorganic phosphate (Pi) levels is crucial for osteoid mineralization. Parathyroid hormone (PTH) mediates actions on phosphate homeostasis mostly by regulating the activity of the type 2 sodium-phosphate cotransporter (Npt2), and this action requires the PDZ protein NHERF1. Osteoblasts express Npt2...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2012.10.001

    authors： Wang B,Yang Y,Liu L,Blair HC,Friedman PA

    更新日期：2013-01-01 00:00:00

  • Zinc deficiency suppresses matrix mineralization and retards osteogenesis transiently with catch-up possibly through Runx 2 modulation.

    abstract：:A characteristic sign of zinc deficiency is retarded skeletal growth, but the role of zinc in osteoblasts is not well understood. Two major events for bone formation include osteoblast differentiation by bone marker gene expression, which is mainly regulated by bone-specific transcription factor Runx2 and extracellula...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2009.11.003

    authors： Kwun IS,Cho YE,Lomeda RA,Shin HI,Choi JY,Kang YH,Beattie JH

    更新日期：2010-03-01 00:00:00

  • Immunochemical characterization of assay for carboxyterminal telopeptide of human type I collagen: loss of antigenicity by treatment with cathepsin K.

    abstract：:The assay for the cross-linked carboxyterminal telopeptide of type I collagen (ICTP) has been shown to reflect increased type I collagen degradation in such pathological conditions as bone metastases and rheumatoid arthritis, but to be rather insensitive to the changes in physiological bone collagen turnover (e.g., in...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/S8756-3282(00)00235-0

    authors： Sassi ML,Eriksen H,Risteli L,Niemi S,Mansell J,Gowen M,Risteli J

    更新日期：2000-04-01 00:00:00

  • Binding and antiresorptive properties of heterocycle-containing bisphosphonate analogs: structure-activity relationships.

    abstract：:To define structure-activity relationships for bisphosphonate activity, we examined the bone binding and antiresorptive properties of heterocycle-containing analogs of risedronate, a pyridylbisphosphonate, in cultures of mouse fetal bone explants. Our studies indicated that hydroxybisphosphonates with the nitrogen mol...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(98)00120-3

    authors： van Beek ER,Löwik CW,Ebetino FH,Papapoulos SE

    更新日期：1998-11-01 00:00:00

  • Vitamin D binding protein genotype is associated with plasma 25OHD concentration in West African children.

    abstract：:Vitamin D is well known for its role in promoting skeletal health. Vitamin D status is determined conventionally by circulating 25-dihydroxyvitamin D (25OHD) concentration. There is evidence indicating that circulating 25OHD concentration is affected by variation in Gc, the gene encoding the vitamin D binding protein ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2014.12.068

    authors： Braithwaite VS,Jones KS,Schoenmakers I,Silver M,Prentice A,Hennig BJ

    更新日期：2015-05-01 00:00:00

  • Reconstruction of the formative site in trabecular bone by a new, quick, and easy method.

    abstract：:Ordinary measurements of the wall thickness (the end result of the osteoblastic work) of trabecular bone packets (completed remodeling sites) describe the average thickness of walls formed during the previous two to three years. If the bone biopsy is obtained shortly after onset of disease or initiation of treatment, ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/8756-3282(92)90004-g

    authors： Steiniche T,Eriksen EF,Kudsk H,Mosekilde L,Melsen F

    更新日期：1992-01-01 00:00:00

  • Evidence for secular change in Paget's disease.

    abstract：:Death certification data has shown that death rates due to Paget's disease of bone and osteosarcoma in older people (assumed to be attributable to Paget's) declined in the latter part of the 19th and in the early 20th century, suggesting that there may be a secular trend toward less severe disease. We have reviewed a ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(96)00310-9

    authors： Cundy T,McAnulty K,Wattie D,Gamble G,Rutland M,Ibbertson HK

    更新日期：1997-01-01 00:00:00

  • Circulating osteogenic cells: characterization and relationship to rates of bone loss in postmenopausal women.

    abstract：:There is increasing evidence that osteogenic cells are present not only in bone marrow (BM) but also in peripheral blood (PB). Since staining for alkaline phosphatase (AP) identifies osteoprogenitor cells in BM, we sought to further characterize BM versus PB hematopoietic lineage negative (lin-)/AP+ cells and to compa...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2010.03.018

    authors： Undale A,Srinivasan B,Drake M,McCready L,Atkinson E,Peterson J,Riggs BL,Amin S,Modder UI,Khosla S

    更新日期：2010-07-01 00:00:00

  • Relationships between quantitative histological measurements and noninvasive assessments of bone mass.

    abstract：:We performed a comprehensive analysis of the relationships between histologic indices in the iliac crest (cancellous bone volume, trabecular structural indices, cortical width, and core width) and bone density in the spine, hip, and wrist in 81 patients with various metabolic bone diseases including osteoporosis, oste...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/8756-3282(92)90203-9

    authors： Cosman F,Schnitzer MB,McCann PD,Parisien MV,Dempster DW,Lindsay R

    更新日期：1992-01-01 00:00:00

  • Shear stress inhibits IL-17A-mediated induction of osteoclastogenesis via osteocyte pathways.

    abstract：:Interleukin (IL)-17 is crucial to osteoclast differentiation and activation. Osteocytes support osteoclast formation and are thought to orchestrate bone remodeling in response to fluid flow. The contribution of IL-17 to osteocyte-related bone resorption remains unclear. Here, we used the osteocyte-like MLO-Y4 cell lin...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2017.04.003

    authors： Liao C,Cheng T,Wang S,Zhang C,Jin L,Yang Y

    更新日期：2017-08-01 00:00:00

  • WISE-2005: bed-rest induced changes in bone mineral density in women during 60 days simulated microgravity.

    abstract：:To better understand the effects of prolonged bed-rest in women, 24 healthy women aged 25 to 40 years participated in 60-days of strict 6° head-down tilt bed-rest (WISE-2005). Subjects were assigned to either a control group (CON, n=8) which performed no countermeasure, an exercise group (EXE, n=8) undertaking a combi...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2011.06.021

    authors： Beller G,Belavý DL,Sun L,Armbrecht G,Alexandre C,Felsenberg D

    更新日期：2011-10-01 00:00:00

  • Systemic bone loss and induction of coronary vessel disease in a rat model of chronic inflammation.

    abstract：:Clinically, osteopenia or low bone mass has been observed in a variety of chronic inflammatory diseases, and elevated proinflammatory mediators have implicated this process. The purpose of this study was to develop an in vivo model of bone loss induced by chronic systemic inflammation. Time-release pellets designed to...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2005.09.008

    authors： Smith BJ,Lerner MR,Bu SY,Lucas EA,Hanas JS,Lightfoot SA,Postier RG,Bronze MS,Brackett DJ

    更新日期：2006-03-01 00:00:00