当前位置： SCI文献检索 > BONE期刊下所有文献 > NHERF1 regulation of PTH-dependent bimodal Pi transport in osteoblasts.

NHERF1 regulation of PTH-dependent bimodal Pi transport in osteoblasts.

Abstract：

:Control of systemic inorganic phosphate (Pi) levels is crucial for osteoid mineralization. Parathyroid hormone (PTH) mediates actions on phosphate homeostasis mostly by regulating the activity of the type 2 sodium-phosphate cotransporter (Npt2), and this action requires the PDZ protein NHERF1. Osteoblasts express Npt2 and in response to PTH enhance osteogenesis by increasing mineralized matrix. The regulation of Pi transport in osteoblasts is poorly understood. To address this gap we characterized PTH-dependent Pi transport and the role of NHERF1 in primary mouse calvarial osteoblasts. Under proliferating conditions osteoblasts express Npt2a, Npt2b, PTH receptor, and NHERF1. Npt2a mRNA expression was lower in calvarial osteoblasts from NHERF1-null mice. Under basal conditions Pi uptake in osteoblasts from wild-type mice was greater than that of knockout mice. PTH inhibited Pi uptake in proliferating osteoblasts from wild-type mice, but not in cells from knockout mice. In vitro induction of mineralization enhanced osteoblast differentiation and increased osterix and osteocalcin expression. Contrary to the results with proliferating osteoblasts, PTH increased Pi uptake and ATP secretion in differentiated osteoblasts from wild-type mice. PTH had no effect on Pi uptake or ATP release in differentiated osteoblasts from knockout mice. NHERF1 regulation of PTH-sensitive Pi uptake in proliferating osteoblasts is mediated by cAMP/PKA and PLC/PKC, while modulation of Pi uptake in differentiated osteoblasts depends only on cAMP/PKA signaling. The results suggest that NHERF1 cooperates with PTH in differentiated osteoblasts to increase matrix mineralization. We conclude that NHERF1 regulates PTH that differentially affects Na-dependent Pi transport at distinct stages of osteoblast proliferation and maturation.

journal_name

Bone

journal_title

Bone

authors

Wang B,Yang Y,Liu L,Blair HC,Friedman PA

doi

10.1016/j.bone.2012.10.001

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

268-77

issue

1

eissn

8756-3282

issn

1873-2763

pii

S8756-3282(12)01299-9

journal_volume

52

pub_type

杂志文章

相关文献

BONE文献大全
  • Granulocyte-macrophage colony-stimulating factor-dependent CD11c-positive cells differentiate into active osteoclasts.

    abstract：:Levels of circulating cytokines are elevated in inflammatory diseases. Previously, it was shown that interleukin (IL-)17A, in synergism with 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] and tumor necrosis factor α (TNFα), induces the release of granulocyte-macrophage colony-stimulating factor (GM-CSF) by murine osteoblasts ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2017.01.036

    authors： Ruef N,Dolder S,Aeberli D,Seitz M,Balani D,Hofstetter W

    更新日期：2017-04-01 00:00:00

  • Whole body vibration improves osseointegration by up-regulating osteoblastic activity but down-regulating osteoblast-mediated osteoclastogenesis via ERK1/2 pathway.

    abstract：:Due to the reduction in bone mass and deterioration in bone microarchitecture, osteoporosis is an important risk factor for impairing implant osseointegration. Recently, low-magnitude, high-frequency (LMHF) vibration (LM: <1×g; HF: 20-90Hz) has been shown to exhibit anabolic, but anti-resorptive effects on skeletal ho...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2014.09.026

    authors： Zhou Y,Guan X,Liu T,Wang X,Yu M,Yang G,Wang H

    更新日期：2015-02-01 00:00:00

  • A theoretical analysis of the contributions of remodeling space, mineralization, and bone balance to changes in bone mineral density during alendronate treatment.

    abstract：:In patients with osteoporosis, alendronate treatment causes an increase in bone mineral density (BMD) and a decrease in fracture incidence. Alendronate acts by changing the bone remodeling process. Changes in bone remodeling resulting in decreased remodeling space, increased bone balance per remodeling cycle, and incr...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(01)00613-5

    authors： Hernandez CJ,Beaupré GS,Marcus R,Carter DR

    更新日期：2001-12-01 00:00:00

  • Effect of combined treatment with zoledronic acid and parathyroid hormone on mouse bone callus structure and composition.

    abstract：:In recent years, great interest in combined treatment of parathyroid hormone (PTH) with anti-resorptive therapy has emerged. PTH has been suggested to aid bridging of atrophic fractures and improve strength in closed fracture models. Bisphosphonate treatments typically result in a larger woven bone callus that is slow...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2016.08.012

    authors： Casanova M,Herelle J,Thomas M,Softley R,Schindeler A,Little D,Schneider P,Müller R

    更新日期：2016-11-01 00:00:00

  • Quantification of remodeling parameter sensitivity--assessed by a computer simulation model.

    abstract：:During normal aging and menopause, cancellous bone is lost at all skeletal sites due to remodeling-related factors: negative formation balance; temporarily increased remodeling space; and osteoclastic perforations. The relative importance of the various factors in inducing bone mass loss and perforations is still cont...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(96)00231-1

    authors： Thomsen JS,Mosekilde L,Mosekilde E

    更新日期：1996-11-01 00:00:00

  • Mitf and Tfe3: members of a b-HLH-ZIP transcription factor family essential for osteoclast development and function.

    abstract：:The Microphthalmia-associated transcription factor (Mitf) is required for the proper development of several cell lineages including osteoclasts, melanocytes, retinal pigment epithelial cells, mast cells and natural killer cells. Mutations in Mitf in multiple organisms result in osteopetrosis due to defective osteoclas...

    journal_title：Bone

    pub_type： 杂志文章,评审

    doi：10.1016/j.bone.2003.08.014

    authors： Hershey CL,Fisher DE

    更新日期：2004-04-01 00:00:00

  • Childhood socioeconomic status and adult femoral neck bone strength: findings from the Midlife in the United States Study.

    abstract：PURPOSE:Bone acquisition in childhood impacts adult bone mass, and can be influenced by childhood socioeconomic conditions. Socioeconomic status is also associated with body weight which affects the load that bone is exposed to in a fall. We hypothesized that socioeconomic advantage in childhood is associated with grea...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2013.06.021

    authors： Karlamangla AS,Mori T,Merkin SS,Seeman TE,Greendale GA,Binkley N,Crandall CJ

    更新日期：2013-10-01 00:00:00

  • Subchondral bone dysplasia partly participates in prenatal dexamethasone induced-osteoarthritis susceptibility in female offspring rats.

    abstract：:Prenatal dexamethasone exposure (PDE) induces developmental toxicities of multi-organs and susceptibility to multi-diseases in offspring. However, the effects of PDE on osteoarthritis susceptibility in adult offspring and its mechanism have not been reported. In the present study, we treated pregnant Wistar rats with ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2020.115245

    authors： Xiao H,Xie X,Wen Y,Tan Y,Shangguan Y,Li B,Magdalou J,Wang H,Chen L

    更新日期：2020-04-01 00:00:00

  • International variation in the management of mineral bone disorder in patients with chronic kidney disease: Results from CKDopps.

    abstract：BACKGROUND AND OBJECTIVES:Chronic kidney disease (CKD) is commonly associated with mineral and bone metabolism disorders, but these are less frequently studied in non-dialysis CKD patients than in dialysis patients. We examined and described international variation in mineral and bone disease (MBD) markers and their tr...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2019.115058

    authors： Liabeuf S,McCullough K,Young EW,Pisoni R,Zee J,Reichel H,Pecoits-Filho R,Port FK,Stengel B,Csomor PA,Metzger M,Robinson B,Massy ZA

    更新日期：2019-12-01 00:00:00

  • Monitoring individual morphological changes over time in ovariectomized rats by in vivo micro-computed tomography.

    abstract：:The ovariectomized (OVX) rat is a well established model for osteoporosis research. The recent development of in vivo micro-computed tomography (micro-CT) provides new possibilities to monitor individual bone changes over time. The purpose of this study was to establish the normal time course of bone loss in the OVX r...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2006.04.017

    authors： Boyd SK,Davison P,Müller R,Gasser JA

    更新日期：2006-10-01 00:00:00

  • Mineralization pathways in the active murine epiphyseal growth plate.

    abstract：:Endochondral ossification in the growth plate of long bones involves cartilage mineralization, bone formation and the budding vasculature. Many of these processes take place in a complex and dynamic zone, the provisional ossification zone, of the growth plate. Here we investigate aspects of mineralization in 2D and 3D...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2019.115086

    authors： Haimov H,Shimoni E,Brumfeld V,Shemesh M,Varsano N,Addadi L,Weiner S

    更新日期：2020-01-01 00:00:00

  • Unbiased estimation of vertebral trabecular connectivity in calcium-restricted ovariectomized minipigs.

    abstract：:The topological changes in vertebral cancellous bone were estimated in vertebrae from calcium-restricted ovariectomized Sinclair S-1 minipigs, a recently described animal model of cancellous osteopenia. Connectivity was estimated using unbiased stereological principles in disector pairs of sections from the first lumb...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/8756-3282(95)00116-u

    authors： Boyce RW,Ebert DC,Youngs TA,Paddock CL,Mosekilde L,Stevens ML,Gundersen HJ

    更新日期：1995-06-01 00:00:00

  • A novel auditory ossicles membrane and the development of conductive hearing loss in Dmp1-null mice.

    abstract：:Genetic mouse models are widely used for understanding human diseases but we know much less about the anatomical structure of the auditory ossicles in the mouse than we do about human ossicles. Furthermore, current studies have mainly focused on disease conditions such as osteomalacia and rickets in patients with hypo...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2017.06.007

    authors： Lv K,Huang H,Yi X,Chertoff ME,Li C,Yuan B,Hinton RJ,Feng JQ

    更新日期：2017-10-01 00:00:00

  • Adiponectin as a novel determinant of bone mineral density and visceral fat.

    abstract：:Growing evidence suggests that positive associations between fat mass (FM) and bone mineral density (BMD) are mediated by not only biomechanical but also biochemical factors. Adiponectin is a novel adipocyte-derived hormone that regulates energy homeostasis and has anti-inflammatory and anti-atherogenic effects. Unlik...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(03)00237-0

    authors： Lenchik L,Register TC,Hsu FC,Lohman K,Nicklas BJ,Freedman BI,Langefeld CD,Carr JJ,Bowden DW

    更新日期：2003-10-01 00:00:00

  • Effects of minodronic acid and alendronate on bone remodeling, microdamage accumulation, degree of mineralization and bone mechanical properties in ovariectomized cynomolgus monkeys.

    abstract：:Suppression of bone remodeling by bisphosphonates leads to accumulation of microdamage in bone. If this microdamage develops due to suppressed repair of remodeling only, more potent bisphosphonates should cause more damage. In this study, we evaluated the effects of reduced bone turnover produced by a potent bisphosph...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2013.01.016

    authors： Yamagami Y,Mashiba T,Iwata K,Tanaka M,Nozaki K,Yamamoto T

    更新日期：2013-05-01 00:00:00

  • Sclerostin antibody increases bone mass by stimulating bone formation and inhibiting bone resorption in a hindlimb-immobilization rat model.

    abstract：:Sclerostin monoclonal antibody (Scl-Ab) has been shown to increase bone mass and bone strength by stimulating bone formation in an ovariectomy-induced bone loss rat model. The purpose of this study was to determine the effects of Scl-Ab in a rat immobilization/disuse model in which there was both a decrease in bone fo...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2010.09.009

    authors： Tian X,Jee WS,Li X,Paszty C,Ke HZ

    更新日期：2011-02-01 00:00:00

  • Prostaglandin E2 receptor (EP4) selective agonist (ONO-4819.CD) accelerates bone repair of femoral cortex after drill-hole injury associated with local upregulation of bone turnover in mature rats.

    abstract：:Prostaglandin E(2) (PGE(2)) is essential for fracture healing. Systemic administration of EP4 ligands such as PGE(2) and other synthetic EP4 agonists appears to transduce anabolic signals by binding to receptor EP4. Therefore, the present study was designed to test whether administration of EP4 agonist accelerates the...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2004.01.002

    authors： Tanaka M,Sakai A,Uchida S,Tanaka S,Nagashima M,Katayama T,Yamaguchi K,Nakamura T

    更新日期：2004-06-01 00:00:00

  • Discordant recovery of bone mass and mechanical properties during prolonged recovery from disuse.

    abstract：:Profound bone loss at weight bearing sites is a primary effect of long-duration spaceflight. Moreover, a significant increase in estimated fracture risk remains even 1 year after returning to Earth; hence, it is important to define how quickly bone integrity can recover following prolonged disuse. This study character...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2012.09.021

    authors： Shirazi-Fard Y,Kupke JS,Bloomfield SA,Hogan HA

    更新日期：2013-01-01 00:00:00

  • Bone anabolic effects of subcutaneous treatment with basic fibroblast growth factor alone and in combination with estrogen in osteopenic ovariectomized rats.

    abstract：:Although basic fibroblast growth factor (bFGF) is a potent stimulator of bone formation when administered intravenously, less is known regarding the effects of this peptide on bone following subcutaneous (s.c.) administration. In addition, it is unknown whether coadministration of estrogen enhances the bone response t...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(03)00118-2

    authors： Iwaniec UT,Magee KA,Mitova-Caneva NG,Wronski TJ

    更新日期：2003-09-01 00:00:00

  • Ultrastructural investigations of bone resorptive cells in two types of autosomal dominant osteopetrosis.

    abstract：:In order to investigate the ultrastructure of bone resorptive cells in the two types of adult benign human osteopetrosis, iliac crest biopsies were obtained from 11 patients and 10 normal males, who served as a control group. Six patients had the radiological type I (4 women, 2 men, aged 23-58 years, mean = 36.5 years...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/8756-3282(93)90316-3

    authors： Bollerslev J,Marks SC Jr,Pockwinse S,Kassem M,Brixen K,Steiniche T,Mosekilde L

    更新日期：1993-11-01 00:00:00

  • Intermittent versus continuous clodronate administration in postmenopausal women with low bone mass.

    abstract：:The aim of the study was to compare the effects on bone mass and turnover of continuous vs. intermittent clodronate administration on 120 postmenopausal women (average age 61 years) with low bone mass (femoral neck bone mineral density [BMD] of at least -1 SD or more, T-score), with another 30 women as a control group...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(99)00273-2

    authors： Filipponi P,Cristallini S,Policani G,Schifini MF,Casciari C,Garinei P

    更新日期：2000-03-01 00:00:00

  • Anatomic variations of the lacunar-canalicular system influence solute transport in bone.

    abstract：:Solute transport in the lacunar-canalicular system (LCS) is essential for bone metabolism and mechanotransduction. Using the technique of fluorescence recovery after photobleaching (FRAP) we have been quantifying solute transport in the LCS of murine long bone as a function of loading parameters and molecular size. Ho...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2009.06.026

    authors： Zhou X,Novotny JE,Wang L

    更新日期：2009-10-01 00:00:00

  • Mouse genetic model for bone strength and size phenotypes: NZB/B1NJ and RF/J inbred strains.

    abstract：:The relationships of bone size, bone strength, and bone formation were investigated in two strains of mice, NZB/B1NJ and RF/J. Measurement of the femur midshaft size by peripheral quantitative computed tomography (pQCT) showed that the RF/J mice had a 32% greater cross-sectional area than NZB/B1NJ mice at 10 weeks of ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(02)00908-0

    authors： Wergedal JE,Sheng MH,Ackert-Bicknell CL,Beamer WG,Baylink DJ

    更新日期：2002-12-01 00:00:00

  • Estrogen and calcium supplement use among Japanese-American women: effects upon bone loss when used singly and in combination.

    abstract：:We examined the effects of estrogen and calcium supplementation upon bone loss among a cohort of Japanese-American women. The women averaged 64 years of age (age range 45-81) and 14 years postmenopause at their initial examination in 1981. They returned at 1-3-year intervals for subsequent examinations, most recently ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(95)00249-9

    authors： Davis JW,Ross PD,Johnson NE,Wasnich RD

    更新日期：1995-10-01 00:00:00

  • Hypophosphatemic osteosclerosis, hyperostosis, and enthesopathy associated with novel homozygous mutations of DMP1 encoding dentin matrix protein 1 and SPP1 encoding osteopontin: The first digenic SIBLING protein osteopathy?

    abstract：:The SIBLINGs are a subfamily of the secreted calcium-binding phosphoproteins and comprise five small integrin-binding ligand N-linked glycoproteins [dentin matrix protein-1 (DMP1), secreted phosphoprotein-1 (SPP1) also called osteopontin (OPN), integrin-binding sialoprotein (IBSP) also called bone sialoprotein (BSP), ...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2019.115190

    authors： Whyte MP,Amalnath SD,McAlister WH,McKee MD,Veis DJ,Huskey M,Duan S,Bijanki VN,Alur S,Mumm S

    更新日期：2020-03-01 00:00:00

  • Bone mineral density in Anorexia Nervosa versus Avoidant Restrictive Food Intake Disorder.

    abstract：BACKGROUND:Avoidant Restrictive Food Intake Disorder (ARFID) and Anorexia Nervosa (AN) cause significant underweight in children and young people (CYP). The association of low bone mineral density (BMD) and underweight CYP in AN is well established, but less is known about BMD in ARFID. METHODS:Retrospective case-note...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2020.115307

    authors： Alberts Z,Fewtrell M,Nicholls DE,Biassoni L,Easty M,Hudson LD

    更新日期：2020-05-01 00:00:00

  • Increased sensitivity of rheumatoid synoviocytes to Schnurri-3 expression in TNF-α and IL-17A induced osteoblastic differentiation.

    abstract：OBJECTIVE:To compare the effects of TNF-α and IL-17A on osteogenic differentiation of isolated fibroblast-like synoviocytes (FLS) from healthy donors, osteoarthritis (OA) and rheumatoid arthritis (RA) patients. METHODS:FLS were cultured in osteogenic medium, with and without TNF-α and/or IL-17A. Extracellular matrix m...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2016.04.008

    authors： Lavocat F,Osta B,Miossec P

    更新日期：2016-06-01 00:00:00

  • Binding and antiresorptive properties of heterocycle-containing bisphosphonate analogs: structure-activity relationships.

    abstract：:To define structure-activity relationships for bisphosphonate activity, we examined the bone binding and antiresorptive properties of heterocycle-containing analogs of risedronate, a pyridylbisphosphonate, in cultures of mouse fetal bone explants. Our studies indicated that hydroxybisphosphonates with the nitrogen mol...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/s8756-3282(98)00120-3

    authors： van Beek ER,Löwik CW,Ebetino FH,Papapoulos SE

    更新日期：1998-11-01 00:00:00

  • Estrogen deficiency increases osteoclastogenesis up-regulating T cells activity: a key mechanism in osteoporosis.

    abstract：:Compelling evidences suggest that increased production of osteoclastogenic cytokines by activated T cells plays a relevant role in the bone loss induced by estrogen deficiency in the mouse. However, little information is available on the role of T cells in post-menopausal bone loss in humans. To investigate this issue...

    journal_title：Bone

    pub_type： 杂志文章

    doi：10.1016/j.bone.2008.02.017

    authors： D'Amelio P,Grimaldi A,Di Bella S,Brianza SZM,Cristofaro MA,Tamone C,Giribaldi G,Ulliers D,Pescarmona GP,Isaia G

    更新日期：2008-07-01 00:00:00

  • Bone and muscle: Interactions beyond mechanical.

    abstract：:The musculoskeletal system is significantly more complex than portrayed by traditional reductionist approaches that have focused on and studied the components of this system separately. While bone and skeletal muscle are the two largest tissues within this system, this system also includes tendons, ligaments, cartilag...

    journal_title：Bone

    pub_type： 杂志文章,评审

    doi：10.1016/j.bone.2015.02.010

    authors： Brotto M,Bonewald L

    更新日期：2015-11-01 00:00:00