Dietary Biotin Supplementation Impairs Testis Morphology and Sperm Quality.

Abstract:

:Supplements containing pharmacological concentrations of biotin are commercially available over the counter. Classical toxicity studies have considered biotin administration as harmless; however, recent investigations have shown that biotin supplementation modifies tissue morphology without changes in toxicity markers, raising concerns about the consequences of morphological changes on tissues' functions and the safety of pharmacological concentrations of the vitamin. Testes are very sensitive to toxicants, and testicular histology is a reliable method to study its function. In this work, we investigated the effects of dietary biotin supplementation on testis morphology and spermatogenesis function using an experimental model, in which we have not observed unfavorable effects on other tissue functions or toxicity markers. Male BALB/cAnNHsd mice were fed a control or a biotin-supplemented diet (1.76 or 97.7 mg biotin/kg diet) for 8 weeks. Compared to the control group, the biotin-supplemented mice presented remarkable testis morphology changes, including increased spermatogonia layers; the cellular mechanism involved is related to increased proliferation. Sperm count and serum testosterone levels were not affected, but spermatozoa motility and morphology were significantly impaired in the biotin-supplemented mice. These results caution against the use of supplements with high concentrations of biotin and indicate that biotin's pharmacological effects on morphology need to be considered in toxicological studies.

journal_name

J Med Food

authors

Pastén-Hidalgo K,Riverón-Negrete L,Sicilia-Argumedo G,Canul-Medina G,Salazar-Anzures T,Tapia-Rodríguez M,Hernández-González EO,Roa-Espitia AL,Cedillo-Peláez C,Fernandez-Mejia C

doi

10.1089/jmf.2019.0137

subject

Has Abstract

pub_date

2020-05-01 00:00:00

pages

535-544

issue

5

eissn

1096-620X

issn

1557-7600

journal_volume

23

pub_type

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