Abstract:
:In this article, we report the cloning of an aspartic protease (AP) from flowers of Arctium minus (Hill) Bernh. (Asteraceae) along with the use of depigmented aqueous flower extracts, as a source of APs, for the hydrolysis of whey proteins. The isolated cDNA encoded a protein product with 509 amino acids called arctiumisin, with the characteristic primary structure organization of typical plant APs. Bovine whey protein hydrolysates, obtained employing the enzyme extracts of A. minus flowers, displayed inhibitory angiotensin-converting enzyme (ACE) and antioxidant activities. Hydrolysates after 3 and 5 h of reaction (degree of hydrolysis 2.4 and 5.6, respectively) and the associated peptide fraction with molecular weight below 3 kDa were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, matrix-assisted laser desorption ionization/time of flight mass spectrometry, and reverse phase-high-performance liquid chromatography. The results obtained in this study demonstrate the viability of using proteases from A. minus to increase the antioxidant and inhibitory ACE capacity of whey proteins.
journal_name
J Med Foodjournal_title
Journal of medicinal foodauthors
Cimino CV,Colombo ML,Liggieri C,Bruno M,Vairo-Cavalli Sdoi
10.1089/jmf.2014.0101subject
Has Abstractpub_date
2015-08-01 00:00:00pages
856-64issue
8eissn
1096-620Xissn
1557-7600journal_volume
18pub_type
杂志文章abstract::This study was designed to investigate the anti-obesity and anti-atherosclerotic effects of supplementation with Chungkookjang (CKJ), a traditional fermented soybean food, in overweight/obese subjects. The study was a 12-week, randomized, double-blind, placebo-controlled clinical trial followed by a 3-week screening p...
journal_title:Journal of medicinal food
pub_type: 杂志文章,随机对照试验
doi:10.1089/jmf.2010.1199
更新日期:2011-05-01 00:00:00
abstract::Current experimental studies show that Ocimum tenuiflorum (commonly known as basil or Tulsi) possesses many health benefits. Ocimum is suggested to be antioxidative and anti-inflammatory. Eugenol, an orthomethoxyphenol, and ursolic acid have been identified as important components of basil. Myeloperoxidase (MPO), an o...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2014.0125
更新日期:2015-05-01 00:00:00
abstract::Momordica charantia is used to treat various diseases, including inflammatory conditions. Previous reports indicated that the extract of this plant inhibits activation of nuclear transcription factor-κB (NF-κB) but activates peroxisome proliferator-activated receptor (PPAR). Additionally, cucurbitane-type triterpene g...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2011.1766
更新日期:2012-04-01 00:00:00
abstract::We investigated the effect of kaikasaponin III (KS-III) on Phase I and II enzymes and tissue factor (TF) activity to elucidate the pharmacological actions of this immunosuppressive saponin in the diabetic rat. This compound was obtained from the flower of Pueraria thunbergiana (Leguminosae) by chromatographic isolatio...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/109662004322984671
更新日期:2004-04-01 00:00:00
abstract::This study sought to characterize the effects of some citrus fruit juices (shaddock [Citrus maxima], grapefruit [Citrus paradisii], lemon [Citrus limoni], orange [Citrus sinensis], and tangerine [Citrus reticulata]) on acetylcholinesterase activity in vitro. The total phenolic content, radical scavenging abilities, an...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2011.0226
更新日期:2012-05-01 00:00:00
abstract::The n-3 polyunsaturated fatty acids (PUFAs) are known to reduce risk for coronary heart disease partly by altering blood lipids. The purpose of this study was to determine the effect of low doses of n-3 PUFAs on the lipid profile and their tolerability in mildly hypertriglyceridemic subjects. Subjects with a serum tri...
journal_title:Journal of medicinal food
pub_type: 杂志文章,随机对照试验
doi:10.1089/jmf.2008.1250
更新日期:2009-08-01 00:00:00
abstract::Polyphenols, including green tea catechins, are secondary plant compounds often discussed in the context of health-promoting potential. Evidence for such effects is mainly derived from epidemiological and cell culture studies. The aim of the present study was to investigate antidiabetic, antiadipogenic, and anti-infla...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2012.0205
更新日期:2013-04-01 00:00:00
abstract::Memory impairment is one of main clinical symptoms of brain senescence. To address the effects of Cordyceps militaris Link extract (CE) on memory impairment, a D-galactose (D-Gal)-induced aging mouse model was employed. Mice injected with D-Gal showed a significant learning and memory impairment that was rescued by CE...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2011.2105
更新日期:2012-12-01 00:00:00
abstract::Ilex latifolia Thunb. (Aquifoliaceae), a Chinese bitter tea called "kudingcha," has been widely consumed as a health beverage and found to possess antioxidant, antidiabetic, antihypertensive, anti-inflammatory, and anti-ischemic activities. The aim of the present study was to investigate the neuroprotective effects of...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2015.3443
更新日期:2015-12-01 00:00:00
abstract::Kimchi is a traditional Korean food manufactured by fermenting vegetables with probiotic lactic acid bacteria (LAB). Many bacteria are involved in the fermentation of kimchi, but LAB become dominant while the putrefactive bacteria are suppressed during salting of baechu cabbage and the fermentation. The addition of ot...
journal_title:Journal of medicinal food
pub_type: 杂志文章,评审
doi:10.1089/jmf.2013.3083
更新日期:2014-01-01 00:00:00
abstract::Enzymatic hydrolysates of Laminaria japonica were evaluated for antioxidative activities using hydroxyl radical scavenging activity and protective effects against H(2)O(2)-induced DNA and cell damage. In addition, activities of antioxidative enzymes, including catalase, glutathione peroxidase, and glutathione S-transf...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2007.0675
更新日期:2009-02-01 00:00:00
abstract::Kefir is an association of microrganisms generally grown in milk, with known probiotic activities identified from its soured suspensions. Aqueous media are also able to grow kefir, but little is known about the probiotic properties of its fermented products. This work aimed to evaluate some probiotic properties of a c...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2007.329
更新日期:2008-06-01 00:00:00
abstract::The emergence of antimicrobial resistance, coupled with the availability of fewer antifungal agents with fungicidal actions, prompted this present study to characterize Candida species in our environment and determine the effectiveness of virgin coconut oil as an antifungal agent on these species. In 2004, 52 recent i...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2006.1209
更新日期:2007-06-01 00:00:00
abstract::Garlic protects against degenerative diseases such as hyperlipidemia and cardiovascular diseases. However, raw garlic has a strong pungency, which is unpleasant. In this study, we examined the effect of high temperature/high pressure-processed garlic on plasma lipid profiles in rats. Sprague-Dawley rats were fed a nor...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2011.1922
更新日期:2012-05-01 00:00:00
abstract::Lycopene is one of the major carotenoids and is found almost exclusively in tomatoes and tomato products. Since tomato consumption is associated with decreased risk of prostate cancer, characterizing the effects of lycopene on cell growth or survival, cell cycle progression, and apoptosis in LCNaP human prostate cance...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2004.7.284
更新日期:2004-10-01 00:00:00
abstract::ABSTRACT Recent epidemiological studies have indicated that intake of grapes and other food products such as red wine derived from grapes may lower incidence of cancer and coronary heart diseases. Grapes and grape extracts, in addition to the traditional nutrients such as sugars and vitamins, are also rich sources of ...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2000.3.15
更新日期:2000-04-01 00:00:00
abstract::The transport behavior and absorption mechanism of Ala-His-Leu-Leu (AHLL) intestinal absorption in Caco-2 cell monolayers were clarified systemically. The safe absorptive concentration of AHLL was 200 μg/mL, which was determined by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. The permeat...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2016.3842
更新日期:2017-03-01 00:00:00
abstract::The present study examined, for the first time, the in vitro wound healing potential of chitosan green tea polyphenols (CGP) complex based on the activation of transglutaminase (TGM) genes in epidermal morphogenesis. Response surface methodology was applied to determine the optimal processing condition that gave maxim...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2012.2623
更新日期:2013-06-01 00:00:00
abstract::Long-term therapy with doxorubicin (DOX) is associated with high incidence of cumulative and irreversible dilated cardiomyopathy. The goal of this study was to evaluate the cardioprotective effects and safety of a phlorotannin extract from a brown algae Ecklonia cava (Seapolynol™, SPN) against DOX-induced cardiotoxici...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2017.3919
更新日期:2017-10-01 00:00:00
abstract::Bilberry (European blueberry) has been reported to have many biological effects, including anticancer activity. In this study, we investigated the antiproliferative effects of bilberry extract in relation to its ability to induce apoptosis and affect microtubule assembly and organization in MCF7 human breast cancer ce...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2009.0053
更新日期:2010-04-01 00:00:00
abstract::Extracts obtained from the seeds of Nigella sativa are used as a spice or remedy for the treatment of various inflammatory diseases. The purpose of this study was to examine the analgesic and anti-inflammatory effects of its polyphenols. N. sativa seed polyphenols were prepared, and analgesic and anti-inflammatory eff...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2005.8.488
更新日期:2005-01-01 00:00:00
abstract::Obesity is generally associated with low-grade chronic inflammation that involves the recruitment of macrophages and other inflammation factors to the adipocytes of obese individuals. Tumor necrosis factor-alpha (TNF-α), a cytokine associated with systemic inflammation, is elevated in conditions of obesity. TNF-α is a...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2017.3941
更新日期:2018-03-01 00:00:00
abstract::The underlying causes of denervation of the neuromuscular junction and eventual motor neuron death in amyotrophic lateral sclerosis (ALS) have not been resolved. The superoxide dismutase 1 (SOD1)(G93A) mutant mouse is a frequently used animal model of ALS. We hypothesized that resveratrol (RSV), a polyphenolic molecul...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2009.0243
更新日期:2010-10-01 00:00:00
abstract::Panduratin A, a prenylated chalcone compound, has anti-obesity, anti-bacterial, anti-cancer, anti-inflammatory, and anti-oxidative activities. However, its preventive effect on muscle atrophy has not been studied. The purpose of this study was to evaluate the inhibitory effect of panduratin A on muscle atrophy and to ...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2017.3970
更新日期:2017-11-01 00:00:00
abstract::The quantitative distribution of flavan-3-ols was determined using high-performance liquid chromatography in several grape seed extracts (GSEs). In all GSEs, polymers of four or more carbon units were the group of procyanidins present in the highest concentration, the real quantity ranging between 60% and 99.5%. In a ...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/109662001300341770
更新日期:2001-07-01 00:00:00
abstract::Oxidative stress and hypogonadism are two factors linked to the increased incidence of cardiovascular disease in males. Eating fruits and vegetables is known to reduce the incidences of oxidative stress. The objective of this research was to delineate whether drinking daily squeezed orange juice (OJ) or grapefruit jui...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2006.9.422
更新日期:2006-10-01 00:00:00
abstract::Cassia tora fiber supplement consisting of 2 g of soluble fiber extracted from Cassia semen (C. tora L.), 200 mg of alpha-tocopherol, 500 mg of ascorbic acid, and 300 mg of maltodextrin was formulated in a pack, and given to 15 type II diabetic subjects (seven men and eight women 57.1 +/- 2.9 years old) with instructi...
journal_title:Journal of medicinal food
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1089/jmf.2005.8.311
更新日期:2005-10-01 00:00:00
abstract::Diabetes is a chronic degenerative disease with no cure, is found in millions of people worldwide, and can cause life-threatening complications at any age. The plant Cissus sicyoides L. is a runner plant found abundantly in Brazil, especially in the Amazon. Its therapeutic properties are widely used in popular medicin...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2008.0157
更新日期:2009-08-01 00:00:00
abstract::3,3'-diindolylmethane is a major in vivo metabolite of indole-3-carbinol, a bioactive compound found in cruciferous vegetables. Although 3,3'-diindolylmethane has been implicated to possess antitumorigenic and anti-inflammatory properties, the effect of 3,3'-diindolylmethane on adipogenesis has not been explored previ...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2016.0165
更新日期:2017-07-01 00:00:00
abstract::Several studies have demonstrated a protective effect of estrogen against the risk of developing neurodegenerative diseases; however, the molecular mechanisms involved have not been fully addressed. Isoflavones have been proposed as potential alternatives to estrogen replacement therapy. Therefore, in the present stud...
journal_title:Journal of medicinal food
pub_type: 杂志文章
doi:10.1089/jmf.2008.1069
更新日期:2009-06-01 00:00:00