Abstract:
BACKGROUND:Synaptotagmin 7 (SYT7) is a component of the synaptotagmin family, which is essential in many physiological and pathological processes. In this study, we aimed to investigate the role of SYT7 in osteosarcoma. METHODS:We defined the expression levels of SYT7 in osteosarcoma tissues and para-sarcoma tissues by immunohistochemistry and analyzed the possible correlation between SYT7 expression and pathological characteristics via Mann-Whitney U analysis and Spearman correlation analysis. The effects of SYT7 silencing in vitro cell growth were assessed by MTT assay. Cell cycle and cell apoptosis were assessed by flow cytometry analysis. Wound healing assay and transwell assay were applied to assess the migration and invasion capacity. RESULTS:The results showed that the expression levels of SYT7 were upregulated in osteosarcoma tissues compared with para-sarcoma tissues and positively correlated with the pathological characteristics of osteosarcoma. Functional experiments demonstrated that SYT7 silencing significantly inhibited cell proliferation and colony formation capacity (P<0.001), induced cell cycle arrest which increased the proportion of G2 phase and decreased the proportion of S phase, enhanced cell apoptosis (P<0.01), and limited the capacity of migration and invasion (P<0.01), compared with shCtrl group. CONCLUSION:The results indicated that SYT7 plays a crucial role in the development of osteosarcoma. SYT7 can be applied as a new diagnostic and therapeutic target in osteosarcoma.
journal_name
Histol Histopatholjournal_title
Histology and histopathologyauthors
Wu Z,Sun Z,Huang R,Zang D,Wang C,Yan X,Yan Wdoi
10.14670/HH-18-174subject
Has Abstractpub_date
2020-03-01 00:00:00pages
303-312issue
3eissn
0213-3911issn
1699-5848pii
HH-18-174journal_volume
35pub_type
杂志文章abstract::The terms construct or tissue equivalent refer to neotissue produced by tissue engineering techniques. The elements forming the construct are scaffolds on which cells are "recreated" to form an engineered-tissue sensitive to certain cell signals. The ability of the cells to expand and differentiate on the scaffold is ...
journal_title:Histology and histopathology
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abstract::Immunocytochemical demonstration of metallothionein (MT) has been reported as a useful prognostic tool in human breast cancer. The aim of this study was to determine the immunohistochemical location of MT in canine mammary tumours and its possible correlation with the morphologic characteristics of these tumours. Surg...
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:
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journal_title:Histology and histopathology
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doi:
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journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1987-04-01 00:00:00
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
pub_type: 杂志文章
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journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:
更新日期:1997-07-01 00:00:00
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journal_title:Histology and histopathology
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doi:
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journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-28.277
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journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-15.493
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
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doi:
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
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更新日期:2004-01-01 00:00:00
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journal_title:Histology and histopathology
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journal_title:Histology and histopathology
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更新日期:2004-04-01 00:00:00