Abstract：
:We describe a new technique, decalcification by perfusion, for the softening of bony tissue. The blood circulatory system was perfused in 16 rats via a cannula through the left heart ventricle with a fixative followed by New Decalc (an acidic demineralizer) for 30-240 minutes. Perfusion decalcification for 120 minutes softened all heads and middle ear specimens could be easily sampled and prepared for studies by both light and electron microscope. For comparison, a conventional immersion technique required 72 hours of decalcification to accomplish softening. The perfusion technique considerably reduced the time needed to decalcify the tissue and preserved the morphology better than did the immersion procedure.
journal_name
Histol Histopatholjournal_title
Histology and histopathologyauthors
Nilsson M,Hellström S,Albiin Nsubject
Has Abstractpub_date
1991-07-01 00:00:00pages
415-20issue
3eissn
0213-3911issn
1699-5848journal_volume
6pub_type
杂志文章abstract：:mTOR is a member of the PI3K/Akt/mTOR signaling pathway that participates in cell growth, proliferation, protein synthesis, transcription, angiogenesis, apoptosis and autophagy. mTOR and MAPK pahways are two important key signal pathways which are related to each other. We investigated the role of mTOR and other signa...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-18-258
更新日期：2020-11-01 00:00:00
abstract：:TIMPs in the prostates of male and female gerbils and evaluated the effects of testosterone on the expression of these enzymes. Ventral prostates from male gerbils that were either intact or had been castrated for 7 or 21 days, along with prostates from female gerbils that were either intact or had been treated with t...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-26.1423
更新日期：2011-11-01 00:00:00
abstract：:Autophagy and apoptosis are two important molecular mechanisms that maintain cellular homeostasis under stress conditions. Autophagy represents an intracellular mechanism responsible for turnover of organelles and long-lived proteins through a lysosome-dependent degradation pathway. Cell death signals or sustained str...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-11-714
更新日期：2016-05-01 00:00:00
abstract：:Human epithelium contains keratin, which is expressed during differentiation. Depending on the target cell type, different types of keratin are expressed, and their alterations seem to represent changes in cell properties. The basal cells of oral epithelium express keratin 5 (K5), K14, K15 and K19, but their alteratio...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-27.949
更新日期：2012-07-01 00:00:00
abstract：:Current data on the influence of neuropeptides on the growth, structure and function of cells comprising the hypothalamo-pituitary-adrenal axis were presented and discussed. The action of vasopressin, oxytocin, neurotensin, bombesin, neuropeptide Y, substance P and VIP have been evaluated. The hypothesis has been intr...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：
更新日期：1993-01-01 00:00:00
abstract：:A current concern about the biological effects of electromagnetic fields (EMF) is increasing with the wide spread use of X-band microwaves (MW, 8-10 GHz range). Gigahertz transverse electromagnetic (GTEM) field flat transmission lines are currently being used for experimental exposure of biological samples to high fre...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-26.1187
更新日期：2011-09-01 00:00:00
abstract：:Immunohistochemistry of the terminal complement complex (C5b-9) and fibronectin (FN) is useful to detect myocardial ischemia preceding necrosis in the postmortem diagnosis of sudden cardiac death. The present immunohistochemical study examined connexin43 (Cx43) and zonula occludens-1 (ZO-1) as markers of early myocard...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-29.767
更新日期：2014-06-01 00:00:00
abstract：:Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used for the purpose of anti-inflammation, antipyretic, and analgesia. For this aim, they are used for the alleviation of pain, fever, and inflammation associated with rheumatoid arthritis, sports injuries, and temporary pain. However, treatment with NSAIDs may ...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-27.417
更新日期：2012-04-01 00:00:00
abstract：:Epidermal proliferation, inflammatory changes and microvascular augmentation are prominent features of chronic cutaneous psoriatic lesions. The objective of this study was the investigation of blood and lymphatic microvascular changes in relation to epidermal changes and inflammatory infiltration in dermis. Immunohist...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-11-811
更新日期：2017-05-01 00:00:00
abstract：:Nitric oxide (NO) is a biological messenger molecule produced by one of the essential amino acids L-arginine by the catalytic action of the enzyme NO synthase (NOS). The dual role of NO as a protective or toxic molecule is due to several factors, such as; the isoform of NOS involved, concentration of NO and the type o...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-21.445
更新日期：2006-04-01 00:00:00
abstract：:A method for the in vitro generation of granulomas and its use in the analysis of the human granulomatous response is summarized. As a target for the cellular response L3 larvae of Nippostrongylus brasiliensis are coincubated with human mononuclear blood cells, and within seven to fourteen days the development of bloo...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-16.645
更新日期：2001-04-01 00:00:00
abstract：:The endocrine cells in the gastrointestinal tract of the musk shrew were studied immunohistochemically. Eleven kinds of endocrine cells, immunoreactive for serotonin, somatostatin, gastrin, cholecistokinin, gastric inhibitory polypeptide, motilin, secretin, neurotensin, pancreatic glucagon, enteroglucagon and bovine p...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：BACKGROUND AND RESULTS:Embryos from diabetic mice exhibit several forms of neural tube defects, including non-closure of the neural tube. In the present study, embryos collected at embryonic day 11.5 from diabetic pregnancies displayed open neural tube with architectural disruption of the surrounding tissues. The perce...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-26.965
更新日期：2011-08-01 00:00:00
abstract：:Beta-catenin expression in small cell lung carcinomas (SCLC) was investigated by immunohistochemical method using antibodies against beta-catenin. 50 pre-treatment biopsies were examined and the relationship between beta-catenin expression and the patients' relevant clinical characteristics, response to chemotherapy, ...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-16.353
更新日期：2001-04-01 00:00:00
abstract：:Snake-like chromatin (SLC) is a nuclear alteration occurring under various pathological conditions and in different tissues. The aim of this study was the morphological and immunocytochemical characterization of SLC-positive conjunctival epithelial cells from keratoconjunctivitis sicca (KCS) patients. Impression cytol...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-21.355
更新日期：2006-04-01 00:00:00
abstract：:The recent discovery of nitric oxide (NO) as a biological messenger molecule with unique characteristics has opened a new field in pineal research. This free radical gas is synthesized by the enzyme nitric oxide synthase (NOS) from L-arginine. The activation of adrenoreceptors in the membrane of the pinealocytes media...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：
更新日期：1996-10-01 00:00:00
abstract：:The authors present a case of benign oncocytoma (oxyphil cell adenoma) of the nasal cavity in a young woman. This is the fifth case reported in that region. Immunohistochemical stains revealed positivity only for cytokeratin. ...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:Pneumadin (PNM) is a biologically active decapeptide, which has previously been found to enhanced rat adrenal growth; the mechanism is indirect and probably involves the stimulation of both arginine-vasopressin (AVP) and ACTH release. The effects of 2- and 6-day PNM administration on the atrophic adrenal cortices of r...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：:Glucocorticoids play a major role in attenuation of the inflammatory response and they are useful in the primary combination chemotherapy of breast cancer, since in vitro studies have demonstrated an antiproliferative effect in human breast cancer cells. In contrast, it was recently shown that glucocorticoids protect ...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-23.77
更新日期：2008-01-01 00:00:00
abstract：:Macrophages are important effector cells in cell-mediated immunity against intracellular infection. Among cytokines that macrophages are able to release are IL-12 and TNF alpha. IL-12 is a critical linker between the innate and adaptive cell-mediated immunity, capable of Th1 differentiation and IFN gamma release by T ...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-15.199
更新日期：2000-01-01 00:00:00
abstract：:HPV infection has been identified recently as the causative agent of a subset of squamous cell carcinomas arising in oropharyngeal tonsils. Factors influencing the susceptibility of tonsillar epithelium to HPV-induced oncogenesis are far from being elucidated. A 5-protein signature including cytokeratin (CK)7, anterio...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-11-590
更新日期：2015-07-01 00:00:00
abstract：UNLABELLED:The aim of this work was to evaluate the effect of magnesium sulphate (MgSO4) administration on blood-brain barrier (BBB) permeabilization after cerebral hypoxia-ischemia (HI) induced by partial occlusion of the umbilical cord of premature fetal lambs. We also characterized BBB dysfunction in terms of the le...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-24.1129
更新日期：2009-09-01 00:00:00
abstract：:The purpose of our study was to elucidate pathways of genetically programmed cell death (apoptosis) in corneas with macular dystrophy. 10 corneal buttons (10 patients) with macular dystrophy and 8 buttons (8 patients) from enucleated eyes with chorioideal melanoma (controls) were analysed histologically. Immunohistoch...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-25.287
更新日期：2010-03-01 00:00:00
abstract：:Sample preparation of dental tissues for quantitative electron microprobe analysis has not been critically examined because of the highly mineralized nature of these structures. The present study was designed to establish the most suitable method for the electron probe quantitative determination of calcium in human pe...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-13.109
更新日期：1998-01-01 00:00:00
abstract：:Oligodendrocytes are the myelinating cells of the central nervous system. Since many studies of demyelinating diseases focus their research on this cell type, there is growing interest for obtaining reliable markers that can specifically recognize oligodendroglia. Established markers are the myelin-associated neurite ...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-11-640
更新日期：2015-12-01 00:00:00
abstract：:Transforming growth factor-β (TGF-β) is a key regulator of extracellular matrix (ECM), and may mediate the development of glomerulosclerosis with accumulation of mesangial matrix. Mesangial cells secrete TGF-β in response to common in vitro fibrogenic stimuli. Yet mesangial immunostaining for active TGF-β1 is frequent...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-27.1131
更新日期：2012-09-01 00:00:00
abstract：:The acute and chronic effects of bombesin (BM) on the structure and function of rat adrenal cortex were investigated by morphometric and radioimmunological techniques. An intraperitoneal bolus injection of 2 micrograms/rat BM markedly raised plasma corticosterone (B) concentration (PBC). The intraperitoneal BM infusio...
journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:A variety of studies have shown that incubation of different tumour cell lines with mistletoe lectins (MLs) in vitro has a marked cytotoxic effect. In the concentration range of low cytotoxicity cell death induced by ML-I is quantitatively due to apoptotic processes. The first events observed being membrane perforatio...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-21.285
更新日期：2006-03-01 00:00:00
abstract：:Melanocytes characterized by their tyrosinase activity, melanosomes and dendrites locate in the basal layer of epidermis and hair bulb in the skin of mice. Melanocytes differentiate from undifferentiated melanoblasts derived from embryonic neural crest. Melanocyte-stimulating hormone plays an important role in the reg...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：
更新日期：1995-01-01 00:00:00
abstract：:The repair of articular cartilage defects is increasingly moving into the focus of experimental and clinical investigations. Histological analysis is the gold standard for a valid and objective evaluation of cartilaginous repair tissue and predominantly relies on the use of established scoring systems. In the past thr...
journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-11-620
更新日期：2015-08-01 00:00:00
