Abstract:
:Sample preparation of dental tissues for quantitative electron microprobe analysis has not been critically examined because of the highly mineralized nature of these structures. The present study was designed to establish the most suitable method for the electron probe quantitative determination of calcium in human permanent enamel and dentine while preserving the morphological features. Comparisons of quantitative data obtained with air-drying and freeze-drying methods showed that calcium in enamel was more accurately measured in specimens prepared with cryopreservation and freeze-drying. No significant differences between the methods tested were found in dentine although cryopreservation and freeze-drying yielded less statistical variability. Moreover this approach did not modify morphological features of interest. We recommend this combination of processing techniques for human permanent teeth not only because it was found the most accurate and least variable in determining calcium concentration, but also because of its potential usefulness in studies of alterations in tooth mineralization.
journal_name
Histol Histopatholjournal_title
Histology and histopathologyauthors
Sánchez-Quevedo MC,Nieto-Albano OH,García JM,Gómez de Ferraris ME,Campos Adoi
10.14670/HH-13.109subject
Has Abstractpub_date
1998-01-01 00:00:00pages
109-13issue
1eissn
0213-3911issn
1699-5848journal_volume
13pub_type
杂志文章abstract::The pineal organs of 14-week-old domestic geese were investigated with light and electron microscopy. The pineals consisted of a wide distal part and a narrow middle-proximal one. The glands were attached to the intercommissural region via the choroid plexus. The pineal parenchyma was formed by round or elongated foll...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-21.1075
更新日期:2006-10-01 00:00:00
abstract::Differentiating nonspecific interstitial pneumonia (NSIP) from usual interstitial pneumonia (UIP) is important for the determination of both treatment and prognosis. Using two-dimensional fluorescence difference gel electrophoresis (2D-DIGE), we examined 8 UIPs, 8 NSIPs, and 30 normal lung tissues. Comparisons with co...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-29.377
更新日期:2014-03-01 00:00:00
abstract::The recent discovery of nitric oxide (NO) as a biological messenger molecule with unique characteristics has opened a new field in pineal research. This free radical gas is synthesized by the enzyme nitric oxide synthase (NOS) from L-arginine. The activation of adrenoreceptors in the membrane of the pinealocytes media...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:
更新日期:1996-10-01 00:00:00
abstract::Tumorigenesis correlates with hypermethylation of tumor suppressors and hypomethylation of oncogenes. DNA methyltransferases (DNMTs) catalyze DNA methylation, and mutations and aberrant expression in DNMT genes are found in multiple human tumors. The discovery of the DNA demethylation function of TET proteins has open...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-29.991
更新日期:2014-08-01 00:00:00
abstract::Cocaine- and amphetamine-regulated transcript peptide (CART) is a neuromediator and/or neuromodulator in nerve structures within the gastrointestinal tract, but knowledge about its distribution, functions and co-localisation with other neuronal factors, especially in humans, is very scarce. During the present investig...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-28.851
更新日期:2013-07-01 00:00:00
abstract::In mouse embryos, the expression of Blimp1 has recently revealed a population of allocated primordial germ cell precursors 24 hours earlier than previously thought. Those 'blimped' precursors have been shown to give rise, by mitotic division, to germ cells only and no other cell lineages. Here, we try to understand th...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-23.1553
更新日期:2008-12-01 00:00:00
abstract::A variety of studies have shown that incubation of different tumour cell lines with mistletoe lectins (MLs) in vitro has a marked cytotoxic effect. In the concentration range of low cytotoxicity cell death induced by ML-I is quantitatively due to apoptotic processes. The first events observed being membrane perforatio...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-21.285
更新日期:2006-03-01 00:00:00
abstract::Light and electron microscopy were used to analyze the epithelial lining of odontogenic cysts excised from edentulous regions of the jaws. Clinically, three cases were identified as keratocysts, and 21 cases as cysts other than keratocysts ("non-keratocysts"). The epithelium of the former was found to achieve keratini...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1996-04-01 00:00:00
abstract::The hindgut is the major site in the horse for nutrient digestion and absorption. Most of this activity occurs in the large intestinal compartments, i.e., cecum, right and left ventral colon and left and right dorsal colon. The colonic pelvic flexure is a short and narrow loop connecting the left ventral and left dors...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-15.983
更新日期:2000-07-01 00:00:00
abstract::The adrenal glands of lower vertebrates display a notable intermingling between steroidogenic and chromaffin tissues, which increases from Pisces to Aves. As in mammals, adrenal chromaffin cells contain and release, in addition to catecholamines, serotonin and several peptides, which may affect the secretory activity ...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-13.209
更新日期:1998-01-01 00:00:00
abstract::Earlier we reported that atherosclerotic lesions of apoE-deficient mice contained cells which stained positively with anti-S-100 antibody and that cells exhibiting the ultrastructural features of dendritic cells were present in the aortic lesions. These observations suggested that dendritic cells might be involved in ...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-16.801
更新日期:2001-07-01 00:00:00
abstract::The presence of synaptophysin, a presynaptic Ca2+ binding glycoprotein, has been analyzed in the cochlear nucleus complex of the adult cat using an antisynaptophysin monoclonal antibody. Synaptophysin immunoreactivity was differently distributed between regions of cochlear nuclei. Terminal boutons contacting directly ...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-13.415
更新日期:1998-04-01 00:00:00
abstract::Nitric oxide (NO) is a widely recognized mediator of physiological and pathophysiological signal transmission. Its generation through L-arginine metabolism is relevant in the mesangium of the kidney where NO is produced by constitutive and inducible NO-synthase isoenzymes. Signaling is achieved through target interact...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-15.1151
更新日期:2000-10-01 00:00:00
abstract::F10, a subline of the B16 mouse melanoma cell line, is itself the parent of the more metastatic BL6 line. BL6 cells differ from F10 cells by an alteration of the gene encoding the B56gamma regulatory subunit of protein phosphatase 2A (PP2A), which results in mRNA encoding a truncated variant of the subunit (deltagamma...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-19.391
更新日期:2004-04-01 00:00:00
abstract::We review the morphofunctional characteristics of CD34+ stromal fibroblastic/fibrocytic cells (CD34+ SFCs) and report our observations. We consider the following aspects of CD34+ SFCs: A) The confusing terms applied to this cell type, often combining the prefix CD34 with numerous names, including fibroblasts, fibrocyt...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-29.831
更新日期:2014-07-01 00:00:00
abstract::β-lapachone is a quinone of lapachol extracted from the bark of lapacho tree. Recent findings demonstrated that punched skin wounds of mice healed faster with β-lapachone treatment. The present study investigates the effects of β-lapachone on burn-wound skin of C57BL/6 mice injured by a 100 °C iron stick. Our results ...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-26.905
更新日期:2011-07-01 00:00:00
abstract::In order to reveal modulation of the number of pulmonary neuroendocrine cells (PNEC) in interstitial lung diseases and to clarify significance of cell proliferation activity in occurrence of PNEC, we counted airway PNEC of the patients of idiopathic interstitial pneumonia, secondary interstitial pneumonia and control ...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-17.1121
更新日期:2002-10-01 00:00:00
abstract:BACKGROUND:Protein tyrosine kinase 7 (PTK7) is a member of receptor protein tyrosine kinase-like molecules, which is involved in tumorigenesis. However, the association between PTK7 expression and its pathological significance in survival prognosis remains under investigation. The purpose of this meta-analysis is to cl...
journal_title:Histology and histopathology
pub_type: 杂志文章,meta分析
doi:10.14670/HH-11-933
更新日期:2018-04-01 00:00:00
abstract::Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used for the purpose of anti-inflammation, antipyretic, and analgesia. For this aim, they are used for the alleviation of pain, fever, and inflammation associated with rheumatoid arthritis, sports injuries, and temporary pain. However, treatment with NSAIDs may ...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-27.417
更新日期:2012-04-01 00:00:00
abstract::In the cutaneous nerves, unmyelinated nerve fibers outnumber the myelinated ones but are scarcely analyzed, especially at autopsy. This indifference toward the pathology of unmyelinated nerve fibers may be due to the necessity of electron microscopic analyses and, more importantly, the obscurity of pathological altera...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-15.313
更新日期:2000-01-01 00:00:00
abstract::The effects of diethylstilbestrol (DES) and of long-acting somatostatin analog, octreotide (SMS) on the rat anterior pituitary microvasculature have been studied by means of computer-assisted image analysis. Additionally, the effects of DES and SMS on prolactin secretion and anterior pituitary cell proliferation have ...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1997-10-01 00:00:00
abstract::Fluoxetine shows controversial lung effects as it prevents pulmonary hypertension in adult rats but exposure during gestation causes pulmonary hypertension in neonatal rats. In the present study, we tested the null hypothesis that the antidepressant drug fluoxetine does not modify the development of bronchopulmonary d...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-27.1599
更新日期:2012-12-01 00:00:00
abstract::A number of tumor suppressor and tumor-related genes exhibit promoter hypermethylation with resulting gene silencing in human cancers. In addition, several gene promoters have also been shown to become hypermethylated in non-neoplastic cells during aging. To assess the physiological consequence and clinical significan...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-19.221
更新日期:2004-01-01 00:00:00
abstract::Multilocular cystic renal cell carcinoma (MCRCC) accounts for approximately 1 to 2% of all renal tumors. This tumor is currently classified as a subtype of clear cell RCC. Clinically, the majority of these tumors are incidentally found. Macroscopically, the tumor is well demarcated and consists of various-sized cysts....
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-27.969
更新日期:2012-08-01 00:00:00
abstract::Strain injuries of tendons are the most common orthopedic injuries in athletic subjects, be they equine or human. When the tendon is suddenly damaged, an acute inflammatory phase occurs whereas its repetitive overloading may cause chronic injuries. Currently the criteria used for grading injuries are general and subje...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-11-601
更新日期:2015-08-01 00:00:00
abstract::Prion diseases are characterized by the accumulation of PrPSc, an altered isoform of a normal cellular protein, PrPc. The prion hypothesis holds that the process of conformational change from PrPc to PrPSc under the influence of PrPSc constitutes the basic infectious mechanism in prion diseases. It is still unknown wh...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:
更新日期:1997-07-01 00:00:00
abstract::Processing adult human trabecular bone to obtain tissue sections suitable for research or diagnostic purposes has always been challenging, particularly in the preparation of adult bone specimens for advanced immunohistochemistry applications. In contrast to the majority of soft tissues, decalcified bone samples perfor...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-28.1605
更新日期:2013-12-01 00:00:00
abstract::The effects of cadmium exposure on the protein secretory functions of cultured neonatal rat hepatocytes were analyzed by both two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and electron microscopy. [35S]Methionine-labelled protein secretion was significantly depressed by cadmium exposure in a dose-depend...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::The study aimed at determining levels of metallothionein (MT) and Ki-67 antigen expression in adenocarcinomas of large intestine and examining relation of the expression levels with various clinical and pathological variables. The studies were performed on 81 cases of large intestine adenocarcinoma. Using immunocytoch...
journal_title:Histology and histopathology
pub_type: 临床试验,杂志文章
doi:10.14670/HH-18.401
更新日期:2003-04-01 00:00:00
abstract::Phagocytosis is an important feature of innate immunity in which invading microorganisms are engulfed, killed and degraded - and in some immune cells, their antigens presented to adaptive immune system. A closely related process, efferocytosis, removes apoptotic cells, and is essential for the maintenance of homeostas...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-18-252
更新日期:2020-09-29 00:00:00