当前位置： SCI文献检索 > TRANSPLANTATION PROCEEDINGS期刊下所有文献 > Differential gene expression profile of porcine livers subjected to warm ischemia alone.

Differential gene expression profile of porcine livers subjected to warm ischemia alone.

Abstract：

BACKGROUND:Livers exposed to warm ischemia (WI) are increasingly used for transplantation. The molecular mechanisms activated by WI alone (prior to procurement and transplantation) are not understood. To elucidate the pathways involved, we used microarrays to investigate the gene expression in porcine livers exposed to WI. METHODS:Porcine livers (n = 6 group) were randomly subjected to WI periods of 15, 30 or 45 minutes. mRNA was extracted and gene expression determined by microarray analysis. Using bioinformatics software, we identified differentially expressed genes and related molecular pathways. We used the corresponding human annotation of the porcine microarray for the functional analysis. RESULTS:Between 0 and 15 minutes of WI, 3530 genes were altered with a 2-log-fold change of <-0.58 or >+0.58 and P < .05. Between 0 and 30 minutes of WI, 4141 genes were differentially expressed; and between 0 and 45 minutes of WI, 2814 genes. At each time point, ∼50% of genes were up-regulated, whereas 50% were down-regulated. After pathway mapping, we found that the same pathways were induced for observed clustering of in the three WI periods: cell death, proliferation, inflammation, and metabolism pathways. Among the top genes that were up-regulated after 15 minutes of WI, the majority started to return to but did not reach baseline expression with increasing WI. A similar pattern was observed for the top suppressed genes. CONCLUSIONS:WI causes rapid changes in gene expression that affect several molecular pathways. This phenomenon seems to plateau at 15 to 30 minutes of WI. These new insights in the timing and the nature of molecular pathways induced by WI alone may help to design specific interventions to alter these changes and improve the outcome of livers from cardiac death donors.

journal_name

Transplant Proc

journal_title

Transplantation proceedings

authors

Vekemans K,Balligand E,Liu Q,Heedfeld V,Wylin T,Monbaliu D,Pirenne J,van Pelt J

doi

10.1016/j.transproceed.2011.09.028

subject

Has Abstract

pub_date

2011-11-01 00:00:00

pages

3460-4

issue

eissn

0041-1345

issn

1873-2623

pii

S0041-1345(11)01203-6

journal_volume

pub_type

杂志文章

在线工具

Differential gene expression profile of porcine livers subjected to warm ischemia alone.