Abstract：
:Hepatocyte and various hepatic stem cell transplantations have been studied as alternative therapies to orthotopic liver transplantation for liver injury. The engraftment of transplanted cells into the parenchyma requires transmigration through sinusoidal endothelial cells (SECs), the only cellular barrier. In this study, we constructed a SEC-imaging perfusion culture system that mimics sinusoids with respect to hemorheologic properties. SECs were successfully maintained for 24 hours. Human liver stem cells (HLSCs) were used as a model of transplanted cells for in vitro engraftment to SECs under perfusion culture conditions. Conditions of high shear stress perfusion with 0.34 dyne/cm(2) significantly reduced cell adhesion in contrast to lower shear stress conditions of 0.1 and 0.03 dyne/cm(2). Among the biologic perfusion fluids, namely, fetal bovine serum (FBS), pig plasma, and 5% human albumin solution, HLSCs showed significantly greater attachment to SECs when perfused with FBS, which is well known to contain abundant amounts of adhesion molecules. This biomimetic SEC perfusion culture system may provide a useful tool to study engraftment mechanisms and to evaluate the effects of various enhancers as an alternative to animal models.
journal_name
Transplant Procjournal_title
Transplantation proceedingsauthors
Noh JK,Jung JG,Jang EM,Lee JH,Park HJ,Kim YA,Jung SM,Kwon CH,Lee DH,Lee SKdoi
10.1016/j.transproceed.2012.03.007subject
Has Abstractpub_date
2012-05-01 00:00:00pages
1116-9issue
4eissn
0041-1345issn
1873-2623pii
S0041-1345(12)00227-8journal_volume
44pub_type
杂志文章abstract：:Tolerance was induced in Lewis (LEW) rat renal allograft recipients of Brown Norway kidneys by multiple pretransplant donor-blood transfusions and prior limited cyclosporine A. Rat renal allograft tolerance was associated with the induction of systemic donor T cells (10%), an early phase of nonspecific suppressor-cell...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/s0041-1345(97)00284-4
更新日期：1997-06-01 00:00:00
abstract：:Cytoprotective effects on the liver of somatostatin (ST) and octreotide (OT) have been previously described in normothermic ischemia-reperfusion models. The purpose of this study was an enzymatic and morphological assessment of hepatic cytoprotective effects during extended cold storage. Rat livers were washed in situ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2007.06.008
更新日期：2007-09-01 00:00:00
abstract：:Transplant tourism, travel with the intent of receiving or donating a transplanted organ, has grown immensely in the past decade but is not without risks. Solid organ donors are potential carriers of infection and rates of infection are high in transplant recipients. Returning transplant recipients should be screened ...
journal_title：Transplantation proceedings
pub_type： 杂志文章,评审
doi：10.1016/j.transproceed.2011.06.022
更新日期：2011-07-01 00:00:00
abstract：AIM:This study investigated changes in the mucosal barrier of transplanted intestines with particular emphasis on antigen sampling by Peyer's patches (PPs). METHODS:Heterotopic small bowel transplantation (SBTx) was performed as described previously. C57BL/6 mice were used as donors and BALB/c (allogeneic) or C57BL/6 ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2010.08.057
更新日期：2010-11-01 00:00:00
abstract：:The aim of this study was to evaluate long-term survival after simultaneous pancreas and kidney (SPK) transplantation in relation to function of both grafts. Among 67 recipients who received SPK transplants between 1988 and 2004, 35 had follow-up longer than 18 months, and were divided into: group I (n = 20), recipien...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2005.12.038
更新日期：2006-01-01 00:00:00
abstract：:Neuroendocrine tumors originating from the small bowel frequently metastasize to the lymph nodes and/or liver. Although surgical extirpation of the primary tumor and locoregional metastases epitomizes the management of patients with such tumors, this is not always possible with conventional surgical techniques. Nonres...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2015.01.007
更新日期：2015-04-01 00:00:00
abstract：:Patients referred for heart transplantation evaluation may be accepted for transplantation, or denied due to existing contraindications or judged to be too well. There is little knowledge about long-term outcome in patients considered too well for transplantation. Ninety-five patients (mean age 47 +/- 12 years, 73% me...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2006.07.038
更新日期：2006-10-01 00:00:00
abstract：BACKGROUND:Platelet-derived growth factor (PDGF) and fibroblast growth factor (FGF) seem to play a key role in immunological reactions shortly after heart transplantation (HTx). The aim of this study was to analyze the time course of the expression of PDGF A and B, PDGF-receptor alpha (PDGF-Ralpha) and beta, aFGF, and ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2006.12.018
更新日期：2007-03-01 00:00:00
abstract：BACKGROUND:Compared to tacrolimus, cyclosporine increases cardiovascular risk. Furthermore, tacrolimus has a negative effect on glucose metabolism compared to cyclosporine. This study investigated the effect of the conversion from cyclosporine to tacrolimus for immunosuppressive therapy on glucose metabolism and cardio...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2019.04.081
更新日期：2019-10-01 00:00:00
abstract：:Urologic complications, observed since the beginning of renal transplantation, cause significant morbidity and mortality. In the first few years the procedure was performed, incidence of urologic complications was reported to be 10% to 25%. Recently, the incidence of urologic complications after renal transplantation ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2005.12.116
更新日期：2006-03-01 00:00:00
abstract：:In the years 2001 to 2005 in Poland, 3146 potential deceased donors were referred with 2583 (82%) organs procured and 57 (2%) donors not used due to positive viral markers. According to Polish rules, in every case of possible organ harvest from a deceased donor we test viral markers of anti-HIV I/II, HBsAg, and anti-H...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2007.08.056
更新日期：2007-11-01 00:00:00
abstract：:We looked at mycobacterial infections occurring after a kidney transplant to determine incidence, risk factors, and outcomes. Of 3921 kidney transplants performed between 1984 and 2002, 18 (0.45%) (10 men, eight women; 11 cadaveric donor, seven living donor graft) were identified as having mycobacterial infection at s...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2004.12.167
更新日期：2005-03-01 00:00:00
abstract：BACKGROUND:On March 11, 2011, our hospital was severely damaged by the Great East Japan Earthquake. We report the rare case of a 5-month-old patient with hepatic artery thrombosis (HAT) after living donor liver transplantation (LDLT), who survived the earthquake that occurred 3 days after the reoperation; we were able ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2012.11.023
更新日期：2013-06-01 00:00:00
abstract：:A 26-year-old Caucasian man developed a large lymphocele after living-related kidney transplantation necessitating repeated drainage of large volumes every other day owing to ureteral compression. An open laparotomy for internal drainage was unsuccessful because of severe encapsulating peritoneal sclerosis. Prolonged ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2013.02.139
更新日期：2013-09-01 00:00:00
abstract：BACKGROUND:Kidney transplantation is performed as a useful treatment to improve the quality of life (QOL) of patients with end-stage renal failure; however, the correlation between mood status and QOL among recipients post-kidney transplantation have yet to be clarified. METHODS:Sixty-eight post-kidney transplantation...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2018.03.077
更新日期：2018-10-01 00:00:00
abstract：:Anastomosing the renal artery and vein in transplant recipients without a cooling mechanism exposes the kidney to temperatures exceeding the metabolic threshold (15°C to 18°C), at which the protective effects of renal hypothermia are lost. This anastomotic time, or second warm ischemic time, can be deleterious to graf...
journal_title：Transplantation proceedings
pub_type： 杂志文章,评审
doi：10.1016/j.transproceed.2018.09.003
更新日期：2018-12-01 00:00:00
abstract：UNLABELLED:We have previously reported that tolerance to class I disparate lung allografts in miniature swine could be induced using an intensive 12-day course of tacrolimus and that pretransplant sensitization with immunogenic MHC class I allopeptides failed to block the induction of tolerance. We also have previously...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2006.10.064
更新日期：2006-12-01 00:00:00
abstract：:A new model of tissue-engineered artificial autologous human skin developed in Andalusia is currently being transplanted into patients suffering from large burns within the Andalusian Public Healthcare System. This product is considered an advanced therapy medicinal product (ATMP) in Europe, and its clinical use impli...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2019.08.014
更新日期：2019-11-01 00:00:00
abstract：:The high prevalence of heart failure has increased the candidate list for heart transplantation; however, there is a shortage of viable donated organs, which is responsible for the high mortality of patients awaiting a transplantation. Because the marginal donor presents additional risk factors, it is not considered t...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2010.12.047
更新日期：2011-01-01 00:00:00
abstract：:Artificial neural network, a computer-based technology that uses nonlinear statistics to recognize the relationship between input variables and an output variable, has been previously applied to outcome prediction in adult kidney recipients. In this study, we evaluated the effectiveness of a neural network model to pr...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2007.05.026
更新日期：2007-07-01 00:00:00
abstract：BACKGROUND:The Model for End-Stage Liver Disease (MELD) was introduced in 1999 to quantify the 3-month prognosis of cirrhotic patients after a transjugular intrahepatic portosystemic shunt (TIPS). Because of the imbalance between organ donors and patients on the waiting list, the MELD was adopted by the United States i...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2007.07.023
更新日期：2007-10-01 00:00:00
abstract：BACKGROUND:Organ donation during life is an act of great altruism, with unique family, social, economic, and psychological impacts. The group of anesthesiologists involved in this program sought to assess the degree of satisfaction among kidney donors between 2007 and 2008 and, in particular, with the anesthetic approa...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2010.12.043
更新日期：2011-01-01 00:00:00
abstract：UNLABELLED:We generated an human embryonic stem cell (hESC) line to augment chimerism-associated tolerance. A 40-year-old African with chronic glomerulonephritis-chronic renal failure with 100% G6PD enzyme deficiency presented for renal transplantation with a 27-year-old, 6/6 HLA-matched sister as a willing donor. MET...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2006.08.173
更新日期：2006-11-01 00:00:00
abstract：:The most common hepatopathy in end-stage renal disease is chronic hepatitis C virus (HCV) infection, which decreases allograft and patient survival in kidney transplants. Until last year we did not have treatments free of interferon, which was contraindicated after renal transplantation owing to the risk of allograft ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2015.09.047
更新日期：2015-11-01 00:00:00
abstract：:The aim of this study was to investigate the results of renal transplantation in amyloidosis patients compared with those on hemodialysis. We compared a group of 25 patients with systemic amyloidosis and end-stage renal disease (ESRD) treated with renal transplantation with a control group of 30 patients with systemic...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2005.12.051
更新日期：2006-03-01 00:00:00
abstract：:Portopulmonary hypertension (PoPH) is a common and feared complication of end-stage liver disease, and imposes increased risk of perioperative morbidity in the liver transplant patient. Herein, we present the first successful use of Imatinib in the perioperative management of a patient who was not responding to conven...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2009.02.100
更新日期：2009-06-01 00:00:00
abstract：INTRODUCTION:To optimize the use of nontransplantable organs in their own territory, the European Commission, as part of a project led by Italy, has promoted the use of an information technology (IT) portal, the COORENOR portal, developed by the Czech Republic in 2012, which evolved to become FOEDUS in 2015. METHODS:T...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2017.02.034
更新日期：2017-05-01 00:00:00
abstract：:Perfusion parameters (PP) and early transplant outcome data from 332 consecutive ECD type kidneys machine preserved on the Waters RM-3 apparatus were reviewed and analyzed to examine the validity of using suboptimal PPs (renal resistance of .41-.60) as a criterion for discarding kidneys. Overall discard rate was 23.5%...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2005.01.058
更新日期：2005-01-01 00:00:00
abstract：:Hypertension is a frequent cardiovascular risk factor in liver transplant recipients. The usefulness of ambulatory blood pressure monitoring (ABPM) in these patients is unknown. This study was aimed at evaluating the circadian rhythms of blood pressure in liver allograft recipients. In 53 liver transplant patients blo...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2005.02.036
更新日期：2005-04-01 00:00:00
abstract：BACKGROUND:Left ventricular hypertrophy, considered an independent factor for cardiovascular mortality, is frequent among renal transplant recipients (RTR), in whom we investigated changes in left ventricular mass (LVM) after grafting and associations with possible causal factors, especially glucose metabolism and oxid...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2012.07.086
更新日期：2012-09-01 00:00:00
