Conditioned taste aversion dependent regulation of amygdala gene expression.

Abstract:

:The present experiments investigated gene expression in the amygdala following contingent taste/LiCl treatment that supports development of conditioned taste aversion (CTA). The use of whole genome chips and stringent data set filtering led to the identification of 168 genes regulated by CTA compared to non-contingent LiCl treatment that does not support CTA learning. Seventy-six of these genes were eligible for network analysis. Such analysis identified "behavior" as the top biological function, which was represented by 15 of the 76 genes. These genes included several neuropeptides, G protein-coupled receptors, ion channels, kinases, and phosphatases. Subsequent qRT-PCR analyses confirmed changes in mRNA expression for 5 of 7 selected genes. We were able to demonstrate directionally consistent changes in protein level for 3 of these genes; insulin 1, oxytocin, and major histocompatibility complex class I-C. Behavioral analyses demonstrated that blockade of central insulin receptors produced a weaker CTA that was less resistant to extinction. Together, these results support the notion that we have identified downstream genes in the amygdala that contribute to CTA learning.

journal_name

Physiol Behav

journal_title

Physiology & behavior

authors

Panguluri SK,Kuwabara N,Kang Y,Cooper N,Lundy RF

doi

10.1016/j.physbeh.2011.11.001

subject

Has Abstract

pub_date

2012-02-28 00:00:00

pages

996-1006

issue

4

eissn

0031-9384

issn

1873-507X

pii

S0031-9384(11)00519-1

journal_volume

105

pub_type

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