Abstract:
INTRODUCTION:In this study we present a reproducible technique to assess motor recovery after nerve injury via neuromuscular junction (NMJ) immunostaining and electrodiagnostic testing. METHODS:Wild-type mice underwent sciatic nerve transection with repair. Hindlimb muscles were collected for microscopy up to 30 weeks after injury. Immunostaining was used to assess axons (NF200), Schwann cells (S100), and motor endplates (α-bungarotoxin). Compound motor action potential (CMAP) amplitude was used to assess tibialis anterior (TA) function. RESULTS:One week after injury, nearly all (98.0%) endplates were denervated. At 8 weeks, endplates were either partially (28.3%) or fully (71.7%) reinnervated. At 16 weeks, NMJ reinnervation reached 87.3%. CMAP amplitude was 83% of naive mice at 16 weeks and correlated with percentage of fully reinnervated NMJs. Morphological differences were noted between injured and noninjured NMJs. DISCUSSION:We present a reproducible method for evaluating NMJ reinnervation. Electrodiagnostic data summarize NMJ recovery. Characterization of wild-type reinnervation provides important data for consideration in experimental design and interpretation.
journal_name
Muscle Nervejournal_title
Muscle & nerveauthors
Vannucci B,Santosa KB,Keane AM,Jablonka-Shariff A,Lu CY,Yan Y,MacEwan M,Snyder-Warwick AKdoi
10.1002/mus.26654subject
Has Abstractpub_date
2019-11-01 00:00:00pages
604-612issue
5eissn
0148-639Xissn
1097-4598journal_volume
60pub_type
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