A lactose-binding lectin from the marine sponge Cinachyrella apion (Cal) induces cell death in human cervical adenocarcinoma cells.


:Cancer represents a set of more than 100 diseases, including malignant tumors from different locations. Strategies inducing differentiation have had limited success in the treatment of established cancers. Marine sponges are a biological reservoir of bioactive molecules, especially lectins. Several animal and plant lectins were purified with antitumor activity, mitogenic, anti-inflammatory and antiviral, but there are few reports in the literature describing the mechanism of action of lectins purified from marine sponges to induce apoptosis in human tumor cells. In this work, a lectin purified from the marine sponge Cinachyrella apion (CaL) was evaluated with respect to its hemolytic, cytotoxic and antiproliferative properties, besides the ability to induce cell death in tumor cells. The antiproliferative activity of CaL was tested against HeLa, PC3 and 3T3 cell lines, with highest growth inhibition for HeLa, reducing cell growth at a dose dependent manner (0.5-10 µg/mL). Hemolytic activity and toxicity against peripheral blood cells were tested using the concentration of IC(50) (10 µg/mL) for both trials and twice the IC(50) for analysis in flow cytometry, indicating that CaL is not toxic to these cells. To assess the mechanism of cell death caused by CaL in HeLa cells, we performed flow cytometry and western blotting. Results showed that lectin probably induces cell death by apoptosis activation by pro-apoptotic protein Bax, promoting mitochondrial membrane permeabilization, cell cycle arrest in S phase and acting as both dependent and/or independent of caspases pathway. These results indicate the potential of CaL in studies of medicine for treating cancer.


Mar Drugs


Marine drugs


Rabelo L,Monteiro N,Serquiz R,Santos P,Oliveira R,Oliveira A,Rocha H,Morais AH,Uchoa A,Santos E




Has Abstract


2012-04-01 00:00:00












  • Leucine aminopeptidase, beta-glucosidase and alkaline phosphatase activity rates and their significance in nutrient cycles in some coastal Mediterranean sites.

    abstract::In aquatic microbial ecology, knowledge of the processes involved in the turnover of organic matter is of utmost importance to understand ecosystem functioning. Microorganisms are major players in the cycling of nutrients (nitrogen, phosphorus) and carbon, thanks to their enzymatic activities (leucine aminopeptidase, ...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Caruso G

    更新日期:2010-03-29 00:00:00

  • Isoaaptamine Induces T-47D Cells Apoptosis and Autophagy via Oxidative Stress.

    abstract::Aaptos is a genus of marine sponge which belongs to Suberitidae and is distributed in tropical and subtropical oceans. Bioactivity-guided fractionation of Aaptos sp. methanolic extract resulted in the isolation of aaptamine, demethyloxyaaptamine, and isoaaptamine. The cytotoxic activity of the isolated compounds was e...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Wu CF,Lee MG,El-Shazly M,Lai KH,Ke SC,Su CW,Shih SP,Sung PJ,Hong MC,Wen ZH,Lu MC

    更新日期:2018-01-09 00:00:00

  • Astaxanthin Counteracts Excitotoxicity and Reduces the Ensuing Increases in Calcium Levels and Mitochondrial Reactive Oxygen Species Generation.

    abstract::Astaxanthin (ASX) is a carotenoid pigment with strong antioxidant properties. We have reported previously that ASX protects neurons from the noxious effects of amyloid-β peptide oligomers, which promote excessive mitochondrial reactive oxygen species (mROS) production and induce a sustained increase in cytoplasmic Ca2...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: García F,Lobos P,Ponce A,Cataldo K,Meza D,Farías P,Estay C,Oyarzun-Ampuero F,Herrera-Molina R,Paula-Lima A,Ardiles ÁO,Hidalgo C,Adasme T,Muñoz P

    更新日期:2020-06-26 00:00:00

  • Comparison of physicochemical characteristics and anticoagulant activities of polysaccharides from three sea cucumbers.

    abstract::In order to search for sulfated polysaccharides in different invertebrate connective tissues and to examine their biological activities, we have isolated three types of polysaccharides from the body wall of the three sea cucumbers Holothuria edulis, Apostichopus japonicas and Holothuria nobilis. The physicochemical pr...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Luo L,Wu M,Xu L,Lian W,Xiang J,Lu F,Gao N,Xiao C,Wang S,Zhao J

    更新日期:2013-02-05 00:00:00

  • Molecular and Morphological Toxicity of Diatom-Derived Hydroxyacid Mixtures to Sea Urchin Paracentrotus lividus Embryos.

    abstract::Oxylipins such as polyunsaturated aldehydes (PUAs) and hydroxyacids (HEPEs) are signaling molecules derived from the oxidation of polyunsaturated fatty acids. They are common in diatoms that constitute a major group of microalgae in freshwater and oceanic ecosystems. Although HEPEs represent the most common oxylipins ...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Albarano L,Ruocco N,Ianora A,Libralato G,Manfra L,Costantini M

    更新日期:2019-03-01 00:00:00

  • Inhibitory effect of N,N-didesmethylgrossularine-1 on inflammatory cytokine production in lipopolysaccharide-stimulated RAW 264.7 cells.

    abstract::N,N-Didesmethylgrossularine-1 (DDMG-1), a compound with a rare alpha-carboline structure, was isolated from an Indonesian ascidian Polycarpa aurata as responsible for the observed inhibitory activity against TNF-alpha production in lipopolysaccharide-stimulated murine macrophage-like RAW264.7 cells. DDMG-1 inhibited t...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Oda T,Lee JS,Sato Y,Kabe Y,Sakamoto S,Handa H,Mangindaan RE,Namikoshi M

    更新日期:2009-11-17 00:00:00

  • Costatone C-A New Halogenated Monoterpene from the New Zealand Red Alga Plocamium angustum.

    abstract::Red algae of the genus Plocamium have been a rich source of halogenated monoterpenes. Herein, a new cyclic monoterpene, costatone C (7), was isolated from the extract of P. angustum collected in New Zealand, along with the previously reported (1E,5Z)-1,6-dichloro-2-methylhepta-1,5-dien-3-ol (8). Elucidation of the pla...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Bracegirdle J,Sohail Z,Fairhurst MJ,Gerth ML,Zuccarello GC,Ali Hashmi M,Keyzers RA

    更新日期:2019-07-19 00:00:00

  • Anti-Diabetic Activity of 2,3,6-Tribromo-4,5-Dihydroxybenzyl Derivatives from Symphyocladia latiuscula through PTP1B Downregulation and α-Glucosidase Inhibition.

    abstract::The marine alga, Symphyocladia latiuscula (Harvey) Yamada, is a good source of bromophenols with numerous biological activities. This study aims to characterize the anti-diabetic potential of 2,3,6-tribromo-4,5-dihydroxybenzyl derivatives isolated from S. latiuscula via their inhibition of tyrosine phosphatase 1B (PTP...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Paudel P,Seong SH,Park HJ,Jung HA,Choi JS

    更新日期:2019-03-14 00:00:00

  • Phytoene Accumulation in the Novel Microalga Chlorococcum sp. Using the Pigment Synthesis Inhibitor Fluridone.

    abstract::Carotenoids are lipophilic pigments found in plants and algae, as well as some bacteria, archaea, and fungi that serve two functions-(1) as light harvesting molecules-primary carotenoids, and (2) as antioxidants, acting against reactive oxygen species⁻secondary carotenoids. Because of their strong antioxidant properti...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Laje K,Seger M,Dungan B,Cooke P,Polle J,Holguin FO

    更新日期:2019-03-22 00:00:00

  • Pharmacokinetic Study of Bioactive Glycopeptide from Strongylocentrotus droebachiensis After Intranasal Administration to Rats Using Biomarker Approach.

    abstract::A glycopeptide fraction (GPF) from internal organs of green sea urchins (Strongylocentrotus droebachiensis Müller, Strongylocentrotidae) has been reported to be an effective bronchitis treatment. In this study, we evaluated the pharmacokinetic and tissue distribution of GPF, following single and repeated intranasal (i...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Shikov AN,Pozharitskaya ON,Faustova NM,Kosman VM,Makarov VG,Razzazi-Fazeli E,Novak J

    更新日期:2019-10-11 00:00:00

  • Sargassum horneri as a Functional Food Ameliorated IgE/BSA-Induced Mast Cell Activation and Passive Cutaneous Anaphylaxis in Mice.

    abstract::Sargassum horneri (S. horneri), an edible brown alga, has been proposed as a functional food with an improvement effect on abnormal skin immune responses. The present study investigates the anti-allergic effect of an ethanol extract from S. horneri (SHE) on immunoglobulin E (IgE)/bovine serum albumin (BSA)-mediated ac...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Han EJ,Kim HS,Sanjeewa KKA,Jung K,Jee Y,Jeon YJ,Fernando IPS,Ahn G

    更新日期:2020-11-26 00:00:00

  • Cyclic marinopyrrole derivatives as disruptors of Mcl-1 and Bcl-x(L) binding to Bim.

    abstract::A series of novel cyclic marinopyrroles were designed and synthesized. Their activity to disrupt the binding of the pro-apoptotic protein, Bim, to the pro-survival proteins, Mcl-1 and Bcl-x(L), was evaluated using ELISA assays. Both atropisomers of marinopyrrole A (1) show similar potency. A tetrabromo congener 9 is t...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Cheng C,Liu Y,Balasis ME,Simmons NL,Li J,Song H,Pan L,Qin Y,Nicolaou KC,Sebti SM,Li R

    更新日期:2014-03-07 00:00:00

  • Seasonal Changes in Mycosporine-Like Amino Acid Production Rate with Respect to Natural Phytoplankton Species Composition.

    abstract::After in situ incubation at the site for a year, phytoplanktons in surface water were exposed to natural light in temperate lakes (every month); thereafter, the net production rate of photoprotective compounds (mycosporine-like amino acids, MAAs) was calculated using (13)C labeled tracer. This is the first report desc...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Ha SY,Lee Y,Kim MS,Kumar KS,Shin KH

    更新日期:2015-11-06 00:00:00

  • Next Generation Sequencing of Actinobacteria for the Discovery of Novel Natural Products.

    abstract::Like many fields of the biosciences, actinomycete natural products research has been revolutionised by next-generation DNA sequencing (NGS). Hundreds of new genome sequences from actinobacteria are made public every year, many of them as a result of projects aimed at identifying new natural products and their biosynth...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审


    authors: Gomez-Escribano JP,Alt S,Bibb MJ

    更新日期:2016-04-13 00:00:00

  • Gracilosulfates A-G, Monosulfated Polyoxygenated Steroids from the Marine Sponge Haliclona gracilis.

    abstract::Seven new polyoxygenated steroids belonging to a new structural group of sponge steroids, gracilosulfates A-G (1-7), possessing 3β-O-sulfonato, 5β,6β epoxy (or 5(6)-dehydro), and 4β,23-dihydroxy substitution patterns as a common structural motif, were isolated from the marine sponge Haliclona gracilis. Their structure...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Shubina LK,Makarieva TN,Denisenko VA,Popov RS,Dyshlovoy SA,Grebnev BB,Dmitrenok PS,von Amsberg G,Stonik VA

    更新日期:2020-08-30 00:00:00

  • Natural Products from Cyanobacteria: Focus on Beneficial Activities.

    abstract::Cyanobacteria are photosynthetic microorganisms that colonize diverse environments worldwide, ranging from ocean to freshwaters, soils, and extreme environments. Their adaptation capacities and the diversity of natural products that they synthesize, support cyanobacterial success in colonization of their respective ec...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审


    authors: Demay J,Bernard C,Reinhardt A,Marie B

    更新日期:2019-05-30 00:00:00

  • Hatsusamides A and B: Two New Metabolites Produced by the Deep-Sea-Derived Fungal Strain Penicillium steckii FKJ-0213.

    abstract::Two new nitrogen-containing metabolites, designated hatsusamide A (1) and B (2), were isolated from a culture broth of Penicilliumsteckii FKJ-0213 together with the known compounds tanzawaic acid B (3) and trichodermamide C (4) by physicochemical (PC) screening. The structures of 1 and 2 were determined as a tanzawaic...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Matsuo H,Hokari R,Ishiyama A,Iwatsuki M,Higo M,Nonaka K,Nagano Y,Takahashi Y,Ōmura S,Nakashima T

    更新日期:2020-10-12 00:00:00

  • Correction: Zhang, G.; Cheng, G.; Jia, P.; Jiao, S.; Feng, C.; Hu, T.; Liu, H.; Du, Y. The Positive Correlation of the Enhanced Immune Response to PCV2 Subunit Vaccine by Conjugation of Chitosan Oligosaccharide with the Deacetylation Degree. Marine Drugs

    abstract::The authors wish to correct Figure 1 in this paper [1] to be as follows:[...]. ...

    journal_title:Marine drugs

    pub_type: 杂志文章,已发布勘误


    authors: Zhang G,Cheng G,Jia P,Jiao S,Feng C,Hu T,Liu H,Du Y

    更新日期:2017-09-20 00:00:00

  • Phylogenetic identification of fungi isolated from the marine sponge Tethya aurantium and identification of their secondary metabolites.

    abstract::Fungi associated with the marine sponge Tethya aurantium were isolated and identified by morphological criteria and phylogenetic analyses based on internal transcribed spacer (ITS) regions. They were evaluated with regard to their secondary metabolite profiles. Among the 81 isolates which were characterized, members o...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Wiese J,Ohlendorf B,Blümel M,Schmaljohann R,Imhoff JF

    更新日期:2011-01-01 00:00:00

  • Fatty Acid Composition and Fatty Acid Associated Gene-Expression in Gilthead Sea Bream (Sparus aurata) are Affected by Low-Fish Oil Diets, Dietary Resveratrol, and Holding Temperature.

    abstract::To sustainably produce marine fish with a high lipid quality rich in omega-3 fatty acids, alternative sources of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are being identified. Moreover, the use of bioactive compounds that would stimulate the in vivo fatty acid synthesis, such as resveratrol (RV), wou...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Torno C,Staats S,Michl SC,de Pascual-Teresa S,Izquierdo M,Rimbach G,Schulz C

    更新日期:2018-10-10 00:00:00

  • Rhodoptilometrin, a Crinoid-Derived Anthraquinone, Induces Cell Regeneration by Promoting Wound Healing and Oxidative Phosphorylation in Human Gingival Fibroblast Cells.

    abstract::Gingival recession (GR) potentially leads to the exposure of tooth root to the oral cavity microenvironment and increases susceptibility to dental caries, dentin hypersensitivity, and other dental diseases. Even though many etiological factors were reported, the specific mechanism of GR is yet to be elucidated. Given ...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Tseng CC,Lai YC,Kuo TJ,Su JH,Sung PJ,Feng CW,Lin YY,Chen PC,Tai MH,Cheng SY,Kuo HM,Wen ZH

    更新日期:2019-02-27 00:00:00

  • Conotoxins that confer therapeutic possibilities.

    abstract::Cone snails produce a distinctive repertoire of venom peptides that are used both as a defense mechanism and also to facilitate the immobilization and digestion of prey. These peptides target a wide variety of voltage- and ligand-gated ion channels, which make them an invaluable resource for studying the properties of...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审


    authors: Essack M,Bajic VB,Archer JA

    更新日期:2012-06-01 00:00:00

  • Preliminary structure-activity relationship on theonellasterol, a new chemotype of FXR antagonist, from the marine sponge Theonella swinhoei.

    abstract::Using theonellasterol as a novel FXR antagonist hit, we prepared a series of semi-synthetic derivatives in order to gain insight into the structural requirements for exhibiting antagonistic activity. These derivatives are characterized by modification at the exocyclic carbon-carbon double bond at C-4 and at the hydrox...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Sepe V,Ummarino R,D'Auria MV,Taglialatela-Scafati O,Marino SD,D'Amore C,Renga B,Chini MG,Bifulco G,Nakao Y,Fusetani N,Fiorucci S,Zampella A

    更新日期:2012-11-05 00:00:00

  • Ochracenoids A and B, guaiazulene-based analogues from gorgonian Anthogorgia ochracea collected from the South China Sea.

    abstract::Two new guaiazulene-based analogues, ochracenoids A (1) and B (2), along with four known analogues (3-6), were isolated from the gorgonian Anthogorgia ochracea collected from the South China Sea. The planar structures of the new compounds were elucidated by comprehensive spectroscopic data. The absolute configuration ...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Zheng JJ,Shao CL,Chen M,Gan LS,Fang YC,Wang XH,Wang CY

    更新日期:2014-03-14 00:00:00

  • Production, Characterization and Biocompatibility Evaluation of Collagen Membranes Derived from Marine Sponge Chondrosia reniformis Nardo, 1847.

    abstract::Collagen is involved in the formation of complex fibrillar networks, providing the structural integrity of tissues. Its low immunogenicity and mechanical properties make this molecule a biomaterial that is extremely suitable for tissue engineering and regenerative medicine (TERM) strategies in human health issues. Her...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Pozzolini M,Scarfì S,Gallus L,Castellano M,Vicini S,Cortese K,Gagliani MC,Bertolino M,Costa G,Giovine M

    更新日期:2018-03-29 00:00:00

  • New lobane and cembrane diterpenes from two comorian soft corals.

    abstract::Preliminary biological investigation of a collection of Comorian soft corals resulted in the selection of two specimens, one of Sarcophyton and the other of Lobophytum, on the basis of their toxicity on larvae of the brine shrimp (Artemia salina) and inhibition of acetylcholinesterase, respectively. Bioassay-guided fr...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Bonnard I,Jhaumeer-Laulloo SB,Bontemps N,Banaigs B,Aknin M

    更新日期:2010-02-23 00:00:00

  • Combination of trabectedin and gemcitabine for advanced soft tissue sarcomas: results of a phase I dose escalating trial of the German Interdisciplinary Sarcoma Group (GISG).

    abstract:BACKGROUND:Evaluation of the potential efficacy and safety of combination therapies for advanced soft tissue sarcomas (STS) has increased substantially after approval of trabectedin and pazopanib. Trabectedin's introduction in Europe in 2007 depended mainly on its activity in so-called L-sarcomas (liposarcoma and leiom...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Kasper B,Reichardt P,Pink D,Sommer M,Mathew M,Rauch G,Hohenberger P

    更新日期:2015-01-13 00:00:00

  • The antihyperlipidemic mechanism of high sulfate content ulvan in rats.

    abstract::Numerous studies have suggested that hyperlipidemia is closely linked to cardiovascular disease. The aim of this study was to investigate the possible antihyperlipidemia mechanism of HU (high sulfate content of ulvan) in high-cholesterol fed rats. Wistar rats were made hyperlipidemic by feeding with a high-cholesterol...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Qi H,Sheng J

    更新日期:2015-05-29 00:00:00

  • Extraction, Identification, Modification, and Antibacterial Activity of Histone from Immature Testis of Atlantic salmon.

    abstract::In the present study, histone from immature testis of Atlantic salmon was extracted and identified, and its antibacterial activity after enzymolysis was investigated. Histone extracted from Atlantic salmon (Salmo salar) testis using the acid extraction method was successfully identified by LC-MS/MS, and revealed signi...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Fu B,Lin H,Ramesh Pavase T,Mi N,Sui J

    更新日期:2020-02-26 00:00:00

  • Haliotis discus discus Sialic Acid-Binding Lectin Reduces the Oncolytic Vaccinia Virus Induced Toxicity in a Glioblastoma Mouse Model.

    abstract::Although oncolytic viruses provide attractive vehicles for cancer treatment, their adverse effects are largely ignored. In this work, rat C6 glioblastoma cells were subcutaneously xenografted into mice, and a thymidine kinase-deficient oncolytic vaccinia virus (oncoVV) induced severe toxicity in this model. However, o...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Li G,Mei S,Cheng J,Wu T,Luo J

    更新日期:2018-04-26 00:00:00