Abstract：
:BMAP-27, a member of cathelicidin family, plays an important role against microorganisms, including bacteria and fungi. BMAP-27 may exert antimicrobial effects through membrane integrity disruption, but the exact molecular mechanism remains unclear. To identify the structural features important for antimicrobial activity and propose a mechanism underlying antibacterial effects, we determined the nuclear magnetic resonance structure of BMAP-27 in a membrane-mimetic environment and investigated its interactions with lipid membranes. BMAP-27 exhibited a long N-terminal α-helix with faces patterned into aromatic and cationic regions, central kink, and short hydrophobic C-terminal helix. While the N-terminal 18-residue peptide (BMAP-18) exerted only antibacterial activity, BMAP-27 showed potent activity against bacteria and cancer cells. Both peptides inhibited bacterial growth, but BMAP-18 showed delayed bactericidal activity and BMAP-27 completely killed bacteria within 20 min. The differences in antimicrobial activities and microbicidal kinetics may be associated with membrane permeabilisation; BMAP-27 rapidly and largely disrupted membrane integrity, whereas BMAP-18 showed low membrane disruption activity. Thus, the N-terminal helix is sufficient to inhibit bacterial growth and the C-terminal helix is involved in membrane permeabilisation for rapid bactericidal and efficient anticancer activities. The structural and functional characterisation of BMAP-27 may encourage the development of novel antimicrobial/anticancer agents.
journal_name
Peptidesjournal_title
Peptidesauthors
Yang S,Lee CW,Kim HJ,Jung HH,Kim JI,Shin SY,Shin SHdoi
10.1016/j.peptides.2019.170106subject
Has Abstractpub_date
2019-08-01 00:00:00pages
170106eissn
0196-9781issn
1873-5169pii
S0196-9781(19)30084-1journal_volume
118pub_type
杂志文章相关文献
PEPTIDES文献大全abstract：:The acute behavioral effects of some neurohypophyseal hormones after intracerebroventricular injection in mice were examined. The effects, consisting mainly of a dose-dependent stimulation of certain behaviors, were demonstrated in three different behavioral tests. The type of the induced behaviors was dependent on th...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(81)80003-4
更新日期：1981-04-01 00:00:00
abstract：:The distributions of neuropeptides in paraffin-embedded tissue sections (PETS) of the eyestalk, brain, and thoracic ganglia of the shrimp Penaeus monodon were visualized by imaging mass spectrometry (IMS). Peptide signals were obtained from PETS without affecting morphological features. Twenty-nine neuropeptides compr...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2011.03.021
更新日期：2012-03-01 00:00:00
abstract：:Previously, we described a gene, flp-1, that encodes seven FMRFamide-like peptides from two alternatively spliced transcripts in the nematode Caenorhabditis elegans. To determine whether all or a subset of the predicted peptides coded for by flp-1 are produced in vivo, we undertook the isolation of FMRFamide-like pept...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(93)90049-m
更新日期：1993-03-01 00:00:00
abstract：:The aim of the present study was to investigate how suckling influences the plasma levels of oxytocin and vasoactive intestinal peptide in lactating dogs. Blood-samples were drawn from ten lactating beagles during suckling, in week one and week three of the five week long lactation period and the levels of vasoactive ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(87)90002-7
更新日期：1987-05-01 00:00:00
abstract：:The peptidome of the central nervous system of adult cabbage root fly, Delia radicum (L) was investigated using matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). Over twenty neuropeptides were identified from three different tissue sources, the combined brain/suboesophageal g...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2010.08.028
更新日期：2011-03-01 00:00:00
abstract：:The last decades have seen no significant progress in extending the survival of lung cancer patients and there is an urgent need to improve current therapies. The substance P (SP)/neurokinin-1 receptor (NK-1R) system plays an important role in the development of cancer: SP and NK-1R antagonists respectively induce cel...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2012.09.024
更新日期：2012-12-01 00:00:00
abstract：:Prothymosin alpha (ProTα), a nuclear protein, plays multiple functions including cell survival. Most recently, we demonstrated that the active 30-amino acid peptide sequence/P30 (amino acids 49-78) in ProTα retains its substantial activity in neuroprotection in vitro and in vivo as well as in the inhibition of cerebra...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2013.02.022
更新日期：2013-05-01 00:00:00
abstract：:Our previous findings that the degree of endotoxin-induced hypotension in the dog is inversely related to ambient temperature (19 degrees through 30 degrees C) and that only increased doses of naloxone are effective at 19 degrees C suggested that opioid activity is also influenced by ambient temperature, increasing in...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(85)90007-5
更新日期：1985-01-01 00:00:00
abstract：:Using the indirect immunofluorescence method, enkephalin-like immunoreactivity was visualized on human fetus spinal cord sections (gestational age from 17 to 25 weeks). Immunolabeled varicose fibers and terminal-like structures were seen through the whole length fetal spinal cord principally in the dorsal gray, in the...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(88)90018-6
更新日期：1988-01-01 00:00:00
abstract：:Bacillus thuringiensis subsp. israelensis (Bti) produces at least four different crystal proteins that are specifically toxic to different mosquito species and that belong to two non-related family of toxins, Cry and Cyt named Cry4Aa, Cry4Ba, Cry11Aa and Cyt1Aa. Cyt1Aa enhances the activity of Cry4Aa, Cry4Ba or Cry11A...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2010.06.005
更新日期：2011-03-01 00:00:00
abstract：:Intracerebroventricular (ICV) and subcutaneous (SC) injections of sauvagine powerfully inhibited gastric acid secretion stimulated by gastric distension and by 2-deoxy-D-glucose, but not by histamine in pylorus-ligated rats. Naloxone failed to antagonize the antisecretory effects of SC and ICV sauvagine. Intravenous i...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(88)90131-3
更新日期：1988-07-01 00:00:00
abstract：:The effects of CCK-8s and desulfated CCK-8 at concentrations ranging from 10(-14) to 10(-6) M were studied in vitro on several functions of human peripheral neutrophils: adherence to substrate, mobility (spontaneous and directed by a chemical gradient or chemotaxis), ingestion of inert particles (latex beads) or cells...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(96)00338-5
更新日期：1997-01-01 00:00:00
abstract：:Antimicrobial peptides (AMPs) secreted by amphibian skin represent an important innate immune defense strategy. There are more than 340 species in the family of Ranidae worldwidely, and from which nearly 100 families of AMPs comprising between 8 and 48 amino acid (aa) residues have been characterized. In current work,...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2012.01.014
更新日期：2012-02-01 00:00:00
abstract：:The analysis of the spectrum of circular dichroism (CD) of methionine-oxidized bovine parathyroid hormone peptide, bPTH(1-34) revealed that approximately 43% of the orderly conformation (alpha-helix and beta-sheet) was converted into random coil structure. This peptide failed to elicit any hypotensive response in rats...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(86)90143-9
更新日期：1986-11-01 00:00:00
abstract：:Urotensin II (UII) induces the development of cardiac remodeling and atherosclerosis by promoting hypertrophy of cardiomyocytes and mitogenesis of fibroblasts and vascular smooth muscle cells. But its effect on cardiac side population cells (CSPs), one of somatic stem cells, is unclear. The present study examined the ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2011.01.024
更新日期：2011-05-01 00:00:00
abstract：:Chemerin is a novel peptide that was identified as a natural ligand for ChemR23. As it has been reported to be involved in the regulation of immune responses and adipogenesis, chemerin may have a variety of physiological functions. Chemerin is synthesized as a precursor (prochemerin) and is proteolytically activated a...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2009.05.030
更新日期：2009-08-01 00:00:00
abstract：:Dissociated neurointermediate lobe cells (75%) bound [biotinyl-Ser1]corticotropin releasing hormone (bio-CRH) and secreted adrenocorticotropin [ACTH(25-39)] in a reverse hemolytic plaque assay (RHPA). The bio-CRH was rapidly internalized (less than 1 min) in vesicles and scattered vacuoles. Plaque percentages increase...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(90)90188-b
更新日期：1990-07-01 00:00:00
abstract：:The leucosulfakinins (LSKs), isolated from head extracts of the cockroach Leucophaea maderae, are sulfated neuropeptides with homology to gastrin and cholecystokinin. The undecapeptide LSK and decapeptide LSK-II stimulate contractions of the isolated cockroach hindgut. Several structural aspects of the two gastrin/CCK...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(88)90237-9
更新日期：1988-01-01 00:00:00
abstract：:Galanin has been shown to be present in substantial quantities in the human and rat genitourinary tract by radioimmunoassay and immunocytochemistry. The highest concentrations measured by radioimmunoassay were found in the human vas deferens, corpus cavernosum and spongiosum and in the vagina and cervix. In man gel ch...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(86)90052-5
更新日期：1986-01-01 00:00:00
abstract：:Pituitary Adenylate-Cyclase Activating Polypeptide (PACAP) and Tac1 gene-encoded tachykinins (substance P: SP, neurokinin A: NKA) are expressed in capsaicin-sensitive nerves, but their role in nociception, inflammation and vasoregulation is unclear. Therefore, we investigated the function of these neuropeptides and th...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2013.03.003
更新日期：2013-05-01 00:00:00
abstract：:Macrophage foam cell formation, characterized by cholesterol ester accumulation catalyzed by acyl-CoA:cholesterol acyltransferase 1 (ACAT1), is the hallmark of early atherogenesis. We previously demonstrated the suppressive effects of incretins, glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic poly...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2013.12.015
更新日期：2014-04-01 00:00:00
abstract：:The interaction of two cathelicidin antimicrobial peptides, LL-37 and SMAP-29, with three bacterial polysaccharides, respectively, produced by Pseudomonas aeruginosa, Burkholderia cepacia and Klebsiella pneumoniae, was investigated to identify possible mechanisms adopted by lung pathogens to escape the action of innat...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2005.01.020
更新日期：2005-07-01 00:00:00
abstract：:We show here that crotamine, a polypeptide from the South American rattlesnake venom with cell penetrating and selective anti-fungal and anti-tumoral properties, presents a potent anti-plasmodial activity in culture. Crotamine inhibits the development of the Plasmodium falciparum parasites in a dose-dependent manner [...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2016.01.013
更新日期：2016-04-01 00:00:00
abstract：:The heptadecapeptide, orphanin FQ or nociceptin (Phe-Gly-Phe-Thr-Gly-Ala-Arg-Lys-Ser-Ala-Arg-Lys-Leu-Ala-Asn-Gln), originally isolated from rat brain has been identified as an endogenous ligand for the orphan opioid-like receptor. Although orphanin FQ shares some sequence and structural homology with kappa-opioid pept...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(97)00037-5
更新日期：1997-01-01 00:00:00
abstract：:Mustard oil, which stimulates small diameter afferents, was used to evoke the expression of the oncogene c-fos in the lumbar spinal cord. C-fos-like immunoreactivity was concentrated in, but not limited to, neuronal nuclei of laminae I and II of the lumbar dorsal horn. Double-label immunocytochemistry was used to dete...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(91)90202-z
更新日期：1991-11-01 00:00:00
abstract：:Urotensin II (UII), first identified from goby urophysis, is a potent vasoactive peptide hormone and an endogenous ligand for an orphan G protein-coupled receptor GPR14, now named urotensin II receptor (UT-R). In addition to its vascular actions, UII has been shown to have mitogenic effects on tumor growth and some re...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2007.06.025
更新日期：2008-05-01 00:00:00
abstract：:The emergence of new and resistant viruses is a serious global burden. Conventional antiviral therapy with small molecules has led to the development of resistant mutants. In the case of hand, foot and mouth disease (HFMD), the absence of a US-FDA approved vaccine calls for urgent need to develop an antiviral that cou...
journal_title：Peptides
pub_type： 杂志文章,评审
doi：10.1016/j.peptides.2020.170443
更新日期：2020-11-07 00:00:00
abstract：:In an immunohistochemical study, a specific antiserum raised against [D-Ala2]deltorphin-I (DADTI), a highly selective ligand for delta opioid receptors, was used to demonstrate immunoreactive structures in the rat retina. [D-Ala2]Deltorphin-I immunoreactivity occurred in a subpopulation of the retinal amacrine cells s...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(94)90169-4
更新日期：1994-01-01 00:00:00
abstract：:Bilateral electrolytic lesions of caudate nucleus were observed to potentiate the cataleptic response, and to prevent the muscular rigidity produced by intracerebroventricular injection or infusion of dermorphin. These results suggest that caudate nucleus plays an inhibitory role in controlling the cataleptic response...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(85)90368-7
更新日期：1985-01-01 00:00:00
abstract：:We sought to establish Endothelin (ET-3) role in the central regulation of bile secretion in the rat. The intracerebroventricular (icv) injection of ET-3 evoked a cholestatic or a choleretic effect depending on the administered dose. Lower doses increased bile flow and bicarbonate excretion, whereas higher doses decre...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2005.02.003
更新日期：2005-07-01 00:00:00
