Abstract：
:Sickle cell disease (SCD) is characterized by the abnormal deformation of red blood cells (RBCs) in the deoxygenated condition, as their elongated shape leads to compromised circulation. The pathophysiology of SCD is influenced by both the biomechanical properties of RBCs and their hemodynamic properties in the microvasculature. A major challenge in the study of SCD involves accurate characterization of the biomechanical properties of individual RBCs with minimum sample perturbation. Here we report the biomechanical properties of individual RBCs from a SCD patient using a non-invasive laser interferometric technique. We optically measure the dynamic membrane fluctuations of RBCs. The measurements are analyzed with a previously validated membrane model to retrieve key mechanical properties of the cells: bending modulus; shear modulus; area expansion modulus; and cytoplasmic viscosity. We find that high cytoplasmic viscosity at ambient oxygen concentration is principally responsible for the significantly decreased dynamic membrane fluctuations in RBCs with SCD, and that the mechanical properties of the membrane cortex of irreversibly sickled cells (ISCs) are different from those of the other types of RBCs in SCD.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Byun H,Hillman TR,Higgins JM,Diez-Silva M,Peng Z,Dao M,Dasari RR,Suresh S,Park Ydoi
10.1016/j.actbio.2012.07.011subject
Has Abstractpub_date
2012-11-01 00:00:00pages
4130-8issue
11eissn
1742-7061issn
1878-7568pii
S1742-7061(12)00312-1journal_volume
8pub_type
杂志文章abstract：:The effects of the microstructures and phases of Zr-rich Mo alloys on their magnetic susceptibilities and mechanical properties were investigated in order to develop a Zr alloy with low magnetic susceptibility for use in magnetic resonance imaging (MRI). The magnetic susceptibility was measured with a magnetic suscept...
journal_title：Acta biomaterialia
pub_type： 杂志文章
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更新日期：2011-12-01 00:00:00
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journal_title：Acta biomaterialia
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更新日期：2015-09-01 00:00:00
abstract：:Crystalline Mg-Zinc (Zn)-Strontium (Sr) ternary alloys consist of elements naturally present in the human body and provide attractive mechanical and biodegradable properties for a variety of biomedical applications. The first objective of this study was to investigate the degradation and cytocompatibility of four Mg-4...
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journal_title：Acta biomaterialia
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更新日期：2014-01-01 00:00:00
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journal_title：Acta biomaterialia
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journal_title：Acta biomaterialia
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更新日期：2009-09-01 00:00:00
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journal_title：Acta biomaterialia
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更新日期：2013-08-01 00:00:00
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journal_title：Acta biomaterialia
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更新日期：2017-03-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2005.12.007
更新日期：2006-05-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2014.07.002
更新日期：2014-11-01 00:00:00
abstract：:Selective adhesion and migration of smooth muscle cells (SMCs) over fibroblasts (FIBs) is required to prevent adventitia fibrosis in vascular regeneration. In this study, a uniform cell-resisting layer of poly(ethylene glycol) (PEG) with a density gradient of azide groups was generated on a substrate by immobilizing t...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.04.005
更新日期：2018-05-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2008.02.003
更新日期：2008-07-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2012.07.004
更新日期：2012-12-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2011.11.026
更新日期：2012-05-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2019.01.022
更新日期：2019-03-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2007.01.003
更新日期：2007-07-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2008.07.004
更新日期：2009-01-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
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更新日期：2020-07-15 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2008.05.007
更新日期：2008-09-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2013.12.027
更新日期：2014-03-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2015.08.004
更新日期：2015-10-01 00:00:00
abstract：:Antibiotic resistance is a major challenge to the treatment of bacterial infections associated with medical devices and biomaterials. One important intrinsic mechanism of such resistance is the formation of persister cells that are phenotypic variants of microorganisms and highly tolerant to antibiotics. Recently, we ...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.12.053
更新日期：2017-03-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.10.039
更新日期：2019-01-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章,评审
doi：10.1016/j.actbio.2012.11.022
更新日期：2013-03-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.05.016
更新日期：2017-07-15 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2019.04.018
更新日期：2019-05-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.10.003
更新日期：2018-12-01 00:00:00
abstract：:Scaffold-free harvest of microtissue with a defined structure has received a great deal of interest in cell-based assay and regenerative medicine. In this study, we developed thermally expandable hydrogels with spatially controlled cell adhesive patterns for rapid harvest of geometrically controlled microtissue. We pa...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.07.040
更新日期：2017-10-01 00:00:00
