Abstract：
:A rapidly expanding literature broadly documents the impact of biophysical cues on cellular behaviors. In spite of increasing research efforts in this field, the underlying signaling processes are poorly understood. One of the candidate molecules for being involved in mechanotransduction is focal adhesion kinase (FAK). To examine the role of FAK in the response of immortalized human corneal epithelial (hTCEpi) cells to topographic cues, FAK was depleted by siRNA transfection. Contrary to expectations, FAK knockdown resulted in an enhanced response with a greater number of hTCEpi cells aligned to the long axis of anisotropically ordered surface ridges and grooves. Both underlying topographic features and FAK depletion modulated the migration of corneal epithelial cells. The impact of FAK knockdown on both migration and alignment varied depending on the topographic cues to which the cells were exposed, with the most significant change observed on the biologically relevant size scale (400nm). Additionally, a change in expression of genes encoding perinuclear Nesprins 1 and 2 (SYNE1, 2) was observed in response to topographic cues. SYNE1/2 expression was also altered by FAK depletion, suggesting that these proteins might represent a link between cytosolic and nuclear signaling processes. The data presented here have relevance to our understanding of the fundamental processes involved in corneal cell behavior to topographic cues. These results highlight the importance of incorporating biophysical cues in the conduction of in vitro studies and into the design and fabrication of implantable prosthetics.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Dreier B,Raghunathan VK,Russell P,Murphy CJdoi
10.1016/j.actbio.2012.07.004subject
Has Abstractpub_date
2012-12-01 00:00:00pages
4285-94issue
12eissn
1742-7061issn
1878-7568pii
S1742-7061(12)00305-4journal_volume
8pub_type
杂志文章abstract：:The boundary layers coating articular cartilage in synovial joints constitute unique biomaterials, providing lubricity at levels unmatched by any human-made materials. The underlying molecular mechanism of this lubricity, essential to joint function, is not well understood. Here we study the interactions between surfa...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.06.043
更新日期：2017-09-01 00:00:00
abstract：:Decellularized nerve, or acellular nerve allografts (ANAs), are an increasingly used alternative to nerve autografts to repair nerve gaps to facilitate regeneration. The adaptive immune system, specifically T cells, plays a role in promoting regeneration upon these ANA scaffolds. However, how T cells promote regenerat...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2020.05.009
更新日期：2020-08-01 00:00:00
abstract：:Three-dimensional (3D) porous zinc (Zn) with a moderate degradation rate is a promising candidate for biodegradable bone scaffolds. However, fabrication of such scaffolds with adequate mechanical properties remains a challenge. Moreover, the composition, crystallography and microstructure of the in vivo degradation pr...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2020.11.032
更新日期：2021-02-01 00:00:00
abstract：:This study investigated the surface characteristics and bone response of titanium implants produced by hydrothermal treatment using H(3)PO(4), and compared them with those of implants produced by commercial surface treatment methods - machining, acid etching, grit blasting, grit blasting/acid etching or spark anodizat...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.02.026
更新日期：2009-07-01 00:00:00
abstract：:Glass-ceramic macroporous scaffolds for tissue engineering have been developed using a polyurethane sponge template and bioactive glass powders. The starting glass (CEL2) belongs to the system SiO(2)-P(2)O(5)-CaO-MgO-Na(2)O-K(2)O and has been synthesised by a conventional melting-quenching route. A slurry of CEL2 powd...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2006.07.012
更新日期：2007-03-01 00:00:00
abstract：:Gd3+-based contrast agents have been extensively used for signal enhancement of T1-weighted magnetic resonance imaging (MRI) due to the large magnetic moment and long electron spin relaxation time of the paramagnetic Gd3+ ion. The key requisites for the development of Gd3+-based contrast agents are their relaxivities ...
journal_title：Acta biomaterialia
pub_type： 杂志文章,评审
doi：10.1016/j.actbio.2020.03.047
更新日期：2020-07-01 00:00:00
abstract：:The development of functional therapies for corneal repair and regeneration is a pressing issue. Corneal stroma provides the principal functions of the cornea. However, because of the highly organized nature of the stromal matrix, the attempts to reproduce corneal stroma might follow a scar model. Here, we have develo...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.06.005
更新日期：2018-07-15 00:00:00
abstract：:Atomic force microscopy (AFM) was used to study the morphological changes of two gram-negative pathogens, Pseudomonas aeruginosa and Escherichia coli, after exposure to nitric oxide (NO). The time-dependent effects of NO released from a xerogel coating and the concentration-dependent effects rendered by a small molecu...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.01.025
更新日期：2009-06-01 00:00:00
abstract：:This paper presents the preparation and absorption performance of iodixanol-imprinted polymers in aqueous and blood plasma media in vitro for biomedical applications. The imprinted polymers were prepared by non-covalent imprinting of iodixanol in a matrix of poly(4-vinylpyridine) crosslinked by ethylene glycol dimetha...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.11.007
更新日期：2010-06-01 00:00:00
abstract：:Tissue growth in bioscaffolds is influenced significantly by pore geometry, but how this geometric dependence emerges from dynamic cellular processes such as cell proliferation and cell migration remains poorly understood. Here we investigate the influence of pore size on the time required to bridge pores in thin 3D-p...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2020.07.010
更新日期：2020-09-15 00:00:00
abstract：:Although the use of biomedical devices in hospital-based care is inevitable, unfortunately, it is also one of the leading causes of the nosocomial infections, and thus demands development of novel antimicrobial materials for medical device fabrication. In the current study, a multi-defense mechanism against Gram-posit...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.05.061
更新日期：2017-08-01 00:00:00
abstract：UNLABELLED:Cellular microenvironments present cells with multiple stimuli, including not only soluble biochemical and insoluble matrix cues but also mechanical factors. Biomaterial array platforms have been used to combinatorially and efficiently probe and define two-dimensional (2D) and 3D microenvironmental cues to g...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2015.11.054
更新日期：2016-04-01 00:00:00
abstract：:Antibiotic resistance is a major challenge to the treatment of bacterial infections associated with medical devices and biomaterials. One important intrinsic mechanism of such resistance is the formation of persister cells that are phenotypic variants of microorganisms and highly tolerant to antibiotics. Recently, we ...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.12.053
更新日期：2017-03-01 00:00:00
abstract：:Many steps are required to generate bone through endochondral ossification with adipose mesenchymal stromal cells (ASC), from cell isolation to in vitro monolayer expansion, seeding into scaffolds, cartilaginous differentiation and in vivo remodeling. Moreover, monolayer expansion and passaging of ASC strongly decreas...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.07.004
更新日期：2018-09-01 00:00:00
abstract：:The usefulness of nanoscale topography in improving vascular response in vitro was established previously on hydrothermally modified titanium surfaces. To propose this strategy of surface modification for translation onto clinically used metallic stents, it is imperative that the surface should be also hemocompatible:...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2013.08.023
更新日期：2013-12-01 00:00:00
abstract：:Puerarin nanoparticles were firstly prepared in the process of solution-enhanced dispersion by supercritical CO(2) (SEDS) and then successfully microencapsulated by poly(l-lactide) (PLLA) in a modified SEDS process. By adding an organic non-solvent, an initial puerarin solution with a higher degree of saturation and l...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.04.032
更新日期：2009-10-01 00:00:00
abstract：:Photochemical internalisation (PCI) is a method for enhancing delivery of drugs to their intracellular target sites of action. In this study we investigated the efficacy of PCI using a porphyrin photosensitiser and a cytotoxic agent on spheroid and non-spheroid compressed collagen 3D constructs of ovarian cancer versu...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.09.041
更新日期：2018-11-01 00:00:00
abstract：:Differentiation of stem cells into functional replacement cells and tissues is a major goal of the regenerative medicine field. However, one limitation has been organization of differentiated cells into multi-cellular, three-dimensional assemblies. The islets of Langerhans contain many endocrine and non-endocrine cell...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2019.08.031
更新日期：2019-10-01 00:00:00
abstract：:Bone replacement using synthetic and degradable materials is desirable in various clinical conditions. Most applied commercial materials are based on hydroxyapatite, which is not chemically degradable under physiological conditions. Here we report the effect of a long-term intramuscular implantation regime on the diss...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2011.05.022
更新日期：2011-09-01 00:00:00
abstract：:We report on new types of chitosan-based nanogels via an ortho ester-based linkage, used as drug carriers for efficient chemotherapy. First, we synthesized a novel diacrylamide containing ortho ester (OEAM) as an acid-labile cross-linker. Subsequently, methacrylated succinyl-chitosan (MASCS) was prepared and polymeriz...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.05.003
更新日期：2017-09-15 00:00:00
abstract：:Bioactive ions released from bioceramics play important roles in bone regeneration; however, it is unclear how each ionic composition in complex bioceramics exerts its specific effect on bone regeneration. The aim of this study is to elucidate the functional effects of Sr and Si ions in bioceramics on the regeneration...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.08.015
更新日期：2017-10-01 00:00:00
abstract：:The use of human mesenchymal stromal cells (hMSC) for treating diseased tissues with poor vascularization has received significant attention, but low cell survival has hampered its translation to the clinic. Bioglasses and glass-ceramics have also been suggested as therapeutic agents for stimulating angiogenesis in so...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.12.009
更新日期：2018-02-01 00:00:00
abstract：:One of the outstanding goals in tissue engineering is to develop a natural coating surface which is easy to manipulate, effective for cell adhesion and fully biocompatible. The ideal surface would be derived from human tissue, perfectly controllable, and pathogen-free, thereby satisfying all of the standards of the he...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.10.018
更新日期：2017-01-15 00:00:00
abstract：:This work investigated the delivery of marrow mesenchymal stem cells (MSCs), with or without the growth factor transforming growth factor-beta1 (TGF-beta1), from biodegradable hydrogel composites on the repair of osteochondral defects in a rabbit model. Three formulations of oligo(poly(ethylene glycol) fumarate) (OPF)...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.07.041
更新日期：2010-01-01 00:00:00
abstract：:Three-dimensional (3-D) cell culture can better mimic physiological conditions in which cells interact with adjacent cells and the extracellular matrix than monolayer culture. We have developed a 3-D cell culture device, the Oxy chip, which can be used to generate and supply oxygen to cell spheroids to prevent hypoxia...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2019.03.001
更新日期：2019-04-01 00:00:00
abstract：:This work focuses on the synthesis and characterization of a novel bioresorbable glass ceramic phosphate-based material (GC-ICEL). More specifically, its solubility in different aqueous media (water, Tris-HCl and acellular simulated body fluid) and the response of human stromal cells cultured on it were investigated. ...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.07.017
更新日期：2010-02-01 00:00:00
abstract：:Critical failures associated with current engineered bone grafts involve insufficient induction of osteogenesis of the implanted cells and lack of vascular integration between graft scaffold and host tissue. This study investigated the combined effects of surface microtextures and biochemical supplements to achieve os...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2015.02.021
更新日期：2015-05-01 00:00:00
abstract：UNLABELLED:Quercetin (3,3',4',5,7-pentahydroxy-flavone) is a flavonoid known for its pharmacological activities, which include antioxidant and anti-inflammatory properties, as well as possible beneficial action on diseases involving bone loss. In this work, we explored the possibility to functionalize hydroxyapatite (H...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2015.12.013
更新日期：2016-03-01 00:00:00
abstract：:Poly(lactic acid) composite hollow spheres containing calcium carbonate were prepared by oil-in-water emulsion evaporation to develop injectable bone substitutes incorporated with cells. The spheres were approximately 1.2mm in diameter and had a shell with a thickness in the range of 50-150microm. The hollow in the sp...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2006.03.002
更新日期：2006-07-01 00:00:00
abstract：:A pH-sensitive copolymer PAsp(DIP)-b-PAsp(MEA) (PDPM) was synthesized and self-assembled to micelle loading chemotherapeutic drug doxorubicin (DOX) and introducing a gold nanocage structure for photothermo-chemotherapy and photoacoustic imaging. After further surface modification with polyethylene glycol (PEG), the DO...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.10.018
更新日期：2017-12-01 00:00:00
