Abstract:
BACKGROUND:In spite of interventions, approximately 1000 per 1,000,000 platelet (PLT) collections are contaminated with bacteria at collection. The current prestorage culture procedure at some blood centers is to inoculate a fixed volume from the collection bag (4-8 mL) regardless of collection volume. The sensitivity of early testing varies with the percent of collection volume sampled. We applied the Poisson model to determine whether sampling larger volumes might increase detection at pertinent contamination levels. STUDY DESIGN AND METHODS:The intervention was testing a fixed proportion of the collection volume from single, double, and triple collections. The Poisson model was applied to blood center data to calculate weighted average detection. Model 1 consisted of inoculating 3.2% of the collection volume from single, 1.6% from double, and 1.2% from triple PLT procedures (8 mL in each case). Model 2 consisted of inoculating 3.8% of the collection volume from all PLT procedures. Volume-related and non-volume-related contamination mechanisms were evaluated. RESULTS:Testing constant proportions of the collection volume (Model 2) increases percent detection over testing constant volumes (Model 1) (68% vs. 41% detection if contamination is 30 colony-forming units (CFUs)/collection bag and 17% vs. 9% detection if contamination is 5 CFUs/collection bag). At low levels of contamination (approx. 5 CFUs/bag), the intervention might double the number of contaminated units detected. CONCLUSION:Based on the application of the Poisson model to detection of bacteria in PLT concentrates, inoculating cultures with slightly consistent proportions of the collection volume should lead to a reduction in false negative tests and in the number of contaminated units transfused.
journal_name
Transfusionjournal_title
Transfusionauthors
Tomasulo PA,Wagner SJdoi
10.1111/j.1537-2995.2012.03821.xsubject
Has Abstractpub_date
2013-04-01 00:00:00pages
835-42issue
4eissn
0041-1132issn
1537-2995journal_volume
53pub_type
杂志文章相关文献
TRANSFUSION文献大全abstract:BACKGROUND:To facilitate volume control in neonates, platelets (PLTs) are aliquoted and stored for short periods in non-gas-permeable syringes before infusion. Although agitation of PLTs during storage in gas-permeable bags is performed to maintain their quality, the effect of syringe agitation during storage is unknow...
journal_title:Transfusion
pub_type: 临床试验,杂志文章
doi:10.1111/j.1537-2995.2011.03230.x
更新日期:2011-12-01 00:00:00
abstract:BACKGROUND:To avoid the need, in serial apheresis donors, either to delay plateletpheresis until a predonation platelet count is completed or to obtain a postdonation count after each procedure, a statistical model has been developed to predict the postdonation platelet count from the donor predonation platelet count, ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1994.34194098605.x
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:The survival of fresh and preserved platelets has been used primarily to determine their therapeutic effectiveness. The function of the fresh and preserved platelets has been difficult to assess. In stable thrombocytopenic patients, platelet function of fresh and preserved allogeneic platelets is evaluated b...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2002.00195.x
更新日期:2002-09-01 00:00:00
abstract:BACKGROUND:The RBC injury that occurs during collection of the first few milliliters of blood into the pH 5.0 ACD (NIH, Formula A) is referred to as the lesion of collection. The RBC injury was evaluated by labeling the ACD RBCs with (51)Cr and measuring the 24-hour posttransfusion survival. The effect of the acidifica...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2002.00071.x
更新日期:2002-03-01 00:00:00
abstract::The possible beneficial role of white cells (WBCs) in donor blood has been investigated with respect to their capacity to remove bacteria. Preparations of buffy coat and whole blood, containing as well as reduced of WBCs, were inoculated with Staphylococcus epidermidis, S. aureus, Escherichia coli, Pseudomonas aerugin...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1991.31791368338.x
更新日期:1991-09-01 00:00:00
abstract:BACKGROUND:Conventional platelet (PLT) storage at room temperature under continuous agitation results in a limited shelf life (5 days) and an increased risk of bacterial contamination. However, both of these aspects can be ameliorated by cold storage. Preliminary work has suggested that PLTs can be cold stored for up t...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15651
更新日期:2020-06-01 00:00:00
abstract::The development of a synthetic medium for platelet storage is an important goal in transfusion medicine. Its use would make large volumes of plasma available for fractionation and might improve the quality of platelets after storage. Several investigators have described successful storage in media containing acetate. ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1993.33493242637.x
更新日期:1993-04-01 00:00:00
abstract::A 27-year-old, gravida 3, para 2 woman experienced a delayed hemolytic transfusion reaction. She had anti-Dob in both serum and eluate 8 days after infusion of 6 units of Do(b+) red cells. No antibody had been detected prior to transfusion. By the 15th day after transfusion, there was no evidence of survival of red ce...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1985.25185116501.x
更新日期:1985-01-01 00:00:00
abstract::Human T-cell leukemia viruses (HTLV-1 and HTLV-2) are associated with a variety of human diseases, including some severe ones. Transfusion transmission of HTLV through cellular blood components is undeniable. HTLV screening of blood donations became mandatory in different countries to improve the safety of blood suppl...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/trf.13329
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:National guidance recommends planning for future blood shortages, but few studies have evaluated how reduced demand could be achieved acutely. STUDY DESIGN AND METHODS:A trained observer collected data concerning red blood cell (RBC) transfusion events outside the operating theater during 68 hours of blood ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2009.02244.x
更新日期:2009-10-01 00:00:00
abstract:BACKGROUND:The RhD blood group antigen is extremely polymorphic and the DEL phenotype represents one such class of polymorphisms. The DEL phenotype prevalent in East Asian populations arises from a synonymous substitution defined as RHD*1227A. However, initially, based on genomic and cDNA studies, the genetic basis for...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14439
更新日期:2018-03-01 00:00:00
abstract:BACKGROUND:This study investigated the effects of a 6-month deferral due to low hemoglobin (Hb) on the subsequent donation patterns of Australian whole blood donors. STUDY DESIGN AND METHODS:The study was a retrospective cohort study of the donation patterns of all whole blood donors deferred for low Hb during a 2-mon...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.02881.x
更新日期:2011-03-01 00:00:00
abstract:BACKGROUND:Guidelines for red blood cell (RBC) transfusions exist; however, transfusion practices vary among centers. This study aimed to analyze transfusion practices and the impact of patients and institutional characteristics on the indications of RBC transfusions in preterm infants. STUDY DESIGN AND METHODS:RBC tr...
journal_title:Transfusion
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1537-2995.2009.02373.x
更新日期:2010-01-01 00:00:00
abstract::Anti-Hy, in a serum of a black man who had received multiple blood transfusions, was found to be a warm reactive and polyclonal IgG antibody which did not bind complement in vitro. 51Cr-labeled Hy-positive red blood cells in the patient showed a shortened "2-component" survival curve. Hy antigens were not detected on ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1975.15676082238.x
更新日期:1975-11-01 00:00:00
abstract::Three cases are presented in which the presence of sodium caprylate dependent albumin auto-agglutinins in the patients' sera resulted in erroneous Rh-hr phenotyping of their erythrocytes. In the initial testing, the albumin control was negative in each case. Since one of the cases was an Rh0(D) negative individual who...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1975.15576082226.x
更新日期:1975-09-01 00:00:00
abstract:BACKGROUND:Qualitative RHD variants are the result of the replacement of RHD exons by their RHCE counterparts or of point mutations in RHD causing amino acid substitutions. For RHD typing, the use of at least two RHD typing polymerase chain reaction (PCR) assays directed at different regions of RHD is advised to preven...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1998.38111299056309.x
更新日期:1998-11-01 00:00:00
abstract::Blood centrifugation, with a continuous-flow or semicontinuous-flow system, was used in the treatment of 17 patients with various hematologic disorders. Total plasma exchange (TPE) controlled symptoms of hyperviscosity and arrested bleeding in three patients. In two patients, TPE made is possible to perform urgent sur...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1977.17578014576.x
更新日期:1977-09-01 00:00:00
abstract::The vasodilative effects associated with the use of sodium acetate have been reported in the literature. Preparations of plasma protein fraction (PPF) substantially free of this vasodilator were developed. This paper presents and discusses experimental work on the improved product. ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1981.21682085754.x
更新日期:1981-11-01 00:00:00
abstract::Fatal postoperative erythroderma (POE) developed in a 52-year-old woman with gallstones who underwent elective cholecystectomy. During surgery, she was transfused with 3 units of unirradiated packed red cells stored in the liquid state for at least 4 days after collection. The POE is believed to have been the result o...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1989.29689318456.x
更新日期:1989-07-01 00:00:00
abstract:BACKGROUND:Evaluation of hemostasis in bleeding patients requires both accuracy and speed. STUDY DESIGN AND METHODS:As an alternative to point-of-care testing, we developed an emergency hemorrhage panel (EHP: prothrombin time [PT], fibrinogen, platelet count, hematocrit) for use in making transfusion decisions on blee...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.02753.x
更新日期:2010-12-01 00:00:00
abstract:BACKGROUND:When bacteria are found, after a platelet transfusion, in the recipient's blood as well as in the platelet concentrate (PC), a causal relationship is normally suspected, with the PC as the causative agent. The other alternative, that the patient has bacteremia and contaminated the PC, is less well documented...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1995.351096026371.x
更新日期:1995-10-01 00:00:00
abstract:BACKGROUND:Marrow stromal cells (MSCs) for clinical trials are inevitably stored before administration, but little is known about the effects of storage on MSCs. The effects of short-term liquid storage on the in vitro function of MSCs intended for a clinical trial were studied. STUDY DESIGN AND METHODS:Early-passage ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2009.02138.x
更新日期:2009-07-01 00:00:00
abstract::Female blood donors with low hematocrit levels detected by copper sulfate screening were selected randomly to receive either 75 mg of iron per day, as ferrous gluconate, or a calcium phosphate placebo. Their ferritin, serum iron, total iron-binding capacity, zinc protoporphyrin, and hemoglobin values, as well as their...
journal_title:Transfusion
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1537-2995.1988.28588337328.x
更新日期:1988-09-01 00:00:00
abstract:BACKGROUND:Umbilical cord blood (UCB) can be used as hematopoietic stem cell source for transplantation. The success of a transplantation is highly correlated with the number of total nucleated cells (TNCs) and CD34+ cells in the UCB. Certain obstetric factors increase the yield of stem cells in the UCB. It is necessar...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2007.01617.x
更新日期:2008-05-01 00:00:00
abstract::Critically ill neonatal infants receive frequent small-volume red cell (RBC) transfusions for replacement of blood drawn for laboratory analysis or for treatment of symptomatic anemia secondary to underlying medical conditions and/or a relative bleeding diathesis. Retrospective review of transfusion practice in a hosp...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1993.33593255596.x
更新日期:1993-05-01 00:00:00
abstract:BACKGROUND:Although a recent study reported shorter survivals in human immunodeficiency virus type 1 (HIV-1)-infected patients who received transfusions, no study has analyzed the relationship in such patients between the frequency of opportunistic infection and transfusion. STUDY DESIGN AND METHODS:Records of 196 HIV...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1994.34194098603.x
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:Variant RHCE alleles with diminished expression of C, c, E, and e antigens have been described and indicate the genetic diversity of this gene locus in several populations. In this study the molecular background of variant RhCE antigens identified by standard serologic routine testing in German blood donors ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2009.02220.x
更新日期:2009-09-01 00:00:00
abstract::ALX148, a novel CD47 blocking agent, is in clinical development for the treatment of advanced solid tumors and lymphoma. Because CD47 is highly expressed on red blood cells (RBCs), its therapeutic blockade can potentially interfere with pretransfusion compatibility testing. This study describes the interference of ALX...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.16009
更新日期:2020-08-20 00:00:00
abstract:BACKGROUND:Bacterial contamination or platelet (PLT) metabolism can change the pH of stored PLT concentrates (PCs). Measurement of pH for quality control is currently done on a limited basis. An easy noninvasive method was developed to obtain sequential pH measurements over time, without risking contamination and/or co...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.02727.x
更新日期:2010-12-01 00:00:00
abstract::In therapeutic plasmapheresis, patient plasma is withdrawn and a colloid replacement solution is infused in its place. A 4% to 5% human serum albumin solution in saline is the preferred replacement solution in most instances, even though this practice causes transient mild deficiencies of most plasma proteins. Albumin...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/j.1537-2995.2012.03623.x
更新日期:2012-05-01 00:00:00