当前位置： SCI文献检索 > JOURNAL OF NEUROSCIENCE METHODS期刊下所有文献 > A novel serum free primary astrocyte culture method that mimic quiescent astrocyte phenotype.

A novel serum free primary astrocyte culture method that mimic quiescent astrocyte phenotype.

Abstract：

BACKGROUND:Primary astrocyte cultures have been used for decades to study astrocyte functions in health and disease. The current primary astrocyte cultures are mostly maintained in serum-containing medium which produces astrocytes with a reactive phenotype as compared to in vivo quiescent astrocytes. The aim of this study was to establish a serum-free astrocyte culture medium that maintains primary astrocytes in a quiescent state. NEW METHOD:Serum free astrocyte base medium (ABM) supplemented with basic fibroblast growth factor 2 (FGF2) and epidermal growth factor (EGF) (ABM-FGF2-EGF) or serum supplemented DMEM (MD-10%FBS) was used to culture primary astrocytes isolated from cerebral cortex of postnatal day 1 C57BL/6 mice. RESULTS:Compared to astrocytes cultured in MD-10%FBS medium, astrocytes in ABM-FGF2-EGF had higher process bearing morphologies similar to in vivo astrocytes. Western blot, immunostaining, quantitative polymerase chain reaction and metabolic assays revealed that astrocytes maintained in ABM-FGF2-EGF had enhanced glycolytic metabolism, higher glycogen content, lower GFAP expression, increased glutamine synthase, and glutamate transporter-1 mRNA levels as compared to astrocytes cultured in MD-10% FBS medium. COMPARISON TO EXISTING METHODS:These observations suggest that astrocytes cultured in ABM-FGF2-EGF media compared to the usual FBS media promote quiescent and biosynthetic phenotype similar to in vivo astrocytes. CONCLUSION:This media provides a novel method for studying astrocytes functions in vitro under physiological and pathological conditions.

journal_name

J Neurosci Methods

journal_title

Journal of neuroscience methods

authors

Prah J,Winters A,Chaudhari K,Hersh J,Liu R,Yang SH

doi

10.1016/j.jneumeth.2019.03.013

subject

Has Abstract

pub_date

2019-05-15 00:00:00

pages

50-63

eissn

0165-0270

issn

1872-678X

pii

S0165-0270(19)30091-3

journal_volume

320

pub_type

杂志文章

相关文献

JOURNAL OF NEUROSCIENCE METHODS文献大全
  • MicroCT optimisation for imaging fascicular anatomy in peripheral nerves.

    abstract：BACKGROUND:Due to the lack of understanding of the fascicular organisation, vagus nerve stimulation (VNS) leads to unwanted off-target effects. Micro-computed tomography (microCT) can be used to trace fascicles from periphery and image fascicular anatomy. NEW METHOD:In this study, we present a simple and reproducible ...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2020.108652

    authors： Thompson N,Ravagli E,Mastitskaya S,Iacoviello F,Aristovich K,Perkins J,Shearing PR,Holder D

    更新日期：2020-05-15 00:00:00

  • Modeling the dopaminergic nerve terminal.

    abstract：:A method is described for developing and evaluating models of neurochemical processes. Computer simulation and simplex optimization are used to examine a model of the dopaminergic nerve terminal of the rat striatum. In the model, synthesis, storage, release, uptake, and metabolism are described by a set of non-linear ...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(88)90045-3

    authors： Justice JB Jr,Nicolaysen LC,Michael AC

    更新日期：1988-01-01 00:00:00

  • The image-combining computer microscope--an interactive instrument for morphometry of the nervous system.

    abstract：:The image-combining computer microscope is a new configuration of light microscope and computer graphics instrumentation which provides remarkable morphometric capabilities over a wide range of applications. The key component is the well known camera lucida, an image-combining device that is used to superimpose the co...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(83)90048-1

    authors： Glaser EM,Tagamets M,McMullen NT,Van der Loos H

    更新日期：1983-05-01 00:00:00

  • A novel telemetry system for recording EEG in small animals.

    abstract：:It has become increasingly evident that continuous EEG monitoring is necessary to observe the development of epilepsy in animals, and to determine the effect of drugs on spontaneous seizures. Telemetric recording systems have been increasingly used to monitor EEG in freely moving animals. One challenge faced by such s...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2011.07.018

    authors： Chang P,Hashemi KS,Walker MC

    更新日期：2011-09-30 00:00:00

  • Direct interaction with no correlation: an experimental pitfall in neural systems.

    abstract：:Neural systems continuously optimize how organisms process their environment and are highly dynamic. Building predictive models of these systems is challenging due to the large number of their elements. Therefore, in experimental and descriptive neurobiology the researcher typically does not seek to catalogue all vari...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2012.02.012

    authors： Janušonis S

    更新日期：2012-01-01 00:00:00

  • Quantitative and qualitative analysis of Wallerian degeneration using restricted axonal labelling in YFP-H mice.

    abstract：:We investigated the usefulness of YFP-H transgenic mice [Neuron 28 (2000) 41] which express yellow fluorescent protein (YFP) in a restricted subset of neurons to study Wallerian degeneration in the PNS. Quantification of YFP positive axons and myelin basic protein (MBP) immunocytochemistry revealed that YFP was random...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2003.10.016

    authors： Beirowski B,Berek L,Adalbert R,Wagner D,Grumme DS,Addicks K,Ribchester RR,Coleman MP

    更新日期：2004-03-15 00:00:00

  • Somatotopic organization of rat thalamocortical slices.

    abstract：:The thalamocortical slice is widely employed for in vitro studies of cortical circuits. This preparation was developed in order to preserve anatomical and functional connectivity between the ventrobasal thalamus and somatosensory (whisker/barrel) cortex of young mice, and thalamocortical slice experiments have contrib...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/s0165-0270(02)00150-4

    authors： Land PW,Kandler K

    更新日期：2002-09-15 00:00:00

  • Trial-to-trial noise cancellation of cortical field potentials in awake macaques by autoregression model with exogenous input (ARX).

    abstract：:Gamma band synchronization has drawn increasing interest with respect to its potential role in neuronal encoding strategy and behavior in awake, behaving animals. However, contamination of these recordings by power line noise can confound the analysis and interpretation of cortical local field potential (LFP). Existin...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2010.10.029

    authors： Wang Z,Roe AW

    更新日期：2011-01-15 00:00:00

  • Flavopiridol reduces the impedance of neural prostheses in vivo without affecting recording quality.

    abstract：:We hypothesized that re-entry into the cell cycle may be associated with reactive gliosis surrounding neural prostheses, and that administration of a cell cycle inhibitor (flavopiridol) at the time of surgery would reduce this effect. We investigated the effects of flavopiridol on recording quality and impedance over ...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2009.06.026

    authors： Purcell EK,Thompson DE,Ludwig KA,Kipke DR

    更新日期：2009-10-15 00:00:00

  • A Deep Learning Approach for Automated Sleep-Wake Scoring in Pre-Clinical Animal Models.

    abstract：BACKGROUND:Experimental investigation of sleep-wake dynamics in animals is an important part of pharmaceutical development. Typically, it involves recording of electroencephalogram, electromyogram, locomotor activity, and electrooculogram. Visual identification, or scoring, of the sleep-wake states from these recording...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2020.108668

    authors： Svetnik V,Wang TC,Xu Y,Hansen BJ,V Fox S

    更新日期：2020-05-01 00:00:00

  • Hybrid wavelet and EMD/ICA approach for artifact suppression in pervasive EEG.

    abstract：BACKGROUND:Electroencephalogram (EEG) signals are often corrupted with unintended artifacts which need to be removed for extracting meaningful clinical information from them. Typically a priori knowledge of the nature of the artifacts is needed for such purpose. Artifact contamination of EEG is even more prominent for ...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2016.04.006

    authors： Bono V,Das S,Jamal W,Maharatna K

    更新日期：2016-07-15 00:00:00

  • Effect of planar microelectrode geometry on neuron stimulation: finite element modeling and experimental validation of the efficient electrode shape.

    abstract：BACKGROUND:Microelectrode arrays have been used successfully for neuronal stimulation both in vivo and in vitro. However, in most instances currents required to activate the neurons have been in un-physiological ranges resulting in neuronal damage and cell death. There is a need to develop electrodes which require less...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2015.03.024

    authors： Ghazavi A,Westwick D,Xu F,Wijdenes P,Syed N,Dalton C

    更新日期：2015-06-15 00:00:00

  • Neurophysiology of neocortical slices resected from children undergoing surgical treatment for epilepsy.

    abstract：:The recent emergence of surgical treatment of childhood epilepsy has led to the accessibility of young human cerebral tissue for electrophysiological studies of the mechanisms involved in epileptogenesis. Intracellular recordings were obtained from neurons in slices prepared from neocortical tissue resected from child...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(94)00193-k

    authors： Dudek FE,Wuarin JP,Tasker JG,Kim YI,Peacock WJ

    更新日期：1995-06-01 00:00:00

  • Isolation of neurons suitable for patch-clamping from adult mammalian central nervous systems.

    abstract：:A method is described for the isolation of neurons from defined regions of the mammalian central nervous system, by a combination of mechanical and enzymatic means. The procedure liberates neurons free of cellular debris and glial investments, allowing the formation of giga-ohm seals with patch clamp electrodes. The c...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(86)90040-3

    authors： Kay AR,Wong RK

    更新日期：1986-05-01 00:00:00

  • A combined in situ hybridization and immunofluorescence procedure allowing visualisation of peptide mRNA and serotonin in single sections.

    abstract：:We describe a novel procedure for combining immunocytochemistry with in situ hybridisation. In contrast to previously published procedures, the technique involves immunofluorescence followed by in situ hybridization and is particularly suitable for antigens which are labile or sensitive to in situ hybridization proces...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/s0165-0270(05)80011-1

    authors： Priestley JV,Wotherspoon G,Savery D,Averill S,Rattray M

    更新日期：1993-06-01 00:00:00

  • Pharmacological validation of a novel home cage activity counter in mice.

    abstract：:Many behavioural tests in rodents are based on the premise that basal locomotor activity of the animals is similar between the tested groups. The measurement of basal home cage activity is therefore an essential parameter, that should be included in all studies which employ tests of anxiety or cognition. Currently ava...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2007.01.008

    authors： Ganea K,Liebl C,Sterlemann V,Müller MB,Schmidt MV

    更新日期：2007-05-15 00:00:00

  • Vibrating glass stylets: tools for precise microsurgery on cuticular structures.

    abstract：:Precisely localized lesions in cuticular structures of insects can be produced by fine glass rods, vibrating transversally at frequencies above 100 kHz. The oscillating edge of the glass rod scrapes off the chitinous material at minimal elastic deformation during treatment. The achieved roughness height is in the 0.1 ...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(89)90111-8

    authors： Gödde J

    更新日期：1989-07-01 00:00:00

  • The use of a muscle graft to repair a segmentary nerve defect. An experimental study using the sciatic nerve of rats as model.

    abstract：:The use of a devitalized skeletal muscle graft and conventional nerve graft to repair a 5mm long segmentary sciatic nerve lesion was studied in rats by means of functional, morphometric and spinal cord motor neuron cell response evaluation. Thirty-four rats were used and divided into four groups according to the proce...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2003.09.010

    authors： Oliveira EF,Mazzer N,Barbieri CH,DelBel EA

    更新日期：2004-02-15 00:00:00

  • Relation between myelin area and axon diameter in the aortic depressor nerve of spontaneously hypertensive rats.

    abstract：:The hypothesis that the aortic depressor nerve (ADN) from spontaneously hypertensive rats (SHR) does not show the expected correlation between myelin sheath area and the axonal diameter of myelinated fibers detected in normotensive rat myelinated fibers was tested by means of regression analysis. Proximal and distal s...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2005.04.007

    authors： Fazan VP,Salgado HC,dos Reis GC,Barreira AA

    更新日期：2005-10-30 00:00:00

  • A novel imaging-compatible sciatic nerve schwannoma model.

    abstract：:Benign schwannomas are common tumors of the cranial and peripheral nerves, causing pain and loss of function. The development of effective therapy for these tumors has been hampered by the lack of relevant experimental in vivo models for convenient testing. Here, we describe a novel schwannoma model in which an immort...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2010.10.021

    authors： Saydam O,Ozdener GB,Senol O,Mizrak A,Prabhakar S,Stemmer-Rachamimov AO,Breakefield XO,Brenner GJ

    更新日期：2011-01-30 00:00:00

  • A new algorithm for drift compensation in multi-unit recordings of action potentials in peripheral autonomic nerves over time.

    abstract：BACKGROUND:Peripheral autonomic nerves control visceral organs and convey information regarding their functional states and are, therefore, potential targets for new therapeutic and diagnostic approaches. Conventionally recorded multi-unit nerve activity in vivo undergoes slow differential drift of signal and noise amp...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章,评审

    doi：10.1016/j.jneumeth.2020.108683

    authors： Davey CE,Soto-Breceda A,Shafton A,McAllen RM,Furness JB,Grayden DB,Stebbing MJ

    更新日期：2020-05-15 00:00:00

  • Multimodal vessel mapping for precise large area alignment of functional optical imaging data to neuroanatomical preparations in marmosets.

    abstract：:Imaging technologies, such as intrinsic optical imaging (IOI), functional magnetic resonance imaging (fMRI) or multiphoton microscopy provide excellent opportunities to study the relationship between functional signals recorded from a cortical area and the underlying anatomical structure. This, in turn, requires accur...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2011.07.029

    authors： Valverde Salzmann MF,Wallace DJ,Logothetis NK,Schüz A

    更新日期：2011-09-30 00:00:00

  • SWDreader: a wavelet-based algorithm using spectral phase to characterize spike-wave morphological variation in genetic models of absence epilepsy.

    abstract：BACKGROUND:Spike-wave discharges (SWD) found in neuroelectrical recordings are pathognomonic to absence epilepsy. The characteristic spike-wave morphology of the spike-wave complex (SWC) constituents of SWDs can be mathematically described by a subset of possible spectral power and phase values. Morlet wavelet transfor...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2014.12.016

    authors： Richard CD,Tanenbaum A,Audit B,Arneodo A,Khalil A,Frankel WN

    更新日期：2015-03-15 00:00:00

  • An analog-digital feedback system for measuring photoreceptor properties with an equal response method.

    abstract：:This paper describes a feedback system in which a neutral density wedge automatically controls the light flux applied to a photoreceptor, so that an intracellular recorded response is held constant. We describe here a "differential sampler" that subtracts the photoreceptors dark response from the light response induce...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(83)90093-6

    authors： Smakman JG,Pijpker BA

    更新日期：1983-08-01 00:00:00

  • Quantitative morphological study of microglial cells in the ischemic rat brain using principal component analysis.

    abstract：:Pathogenic stimuli induce alterations in the morphology of microglial cells. We analysed changes in lectin-stained cells on the 1st, 3rd, 7th or 14th day after transient global ischemia. Three areas differing in the degree of microglial reaction were selected for analysis: the upper cerebral cortex, the hippocampal CA...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2005.01.009

    authors： Soltys Z,Orzylowska-Sliwinska O,Zaremba M,Orlowski D,Piechota M,Fiedorowicz A,Janeczko K,Oderfeld-Nowak B

    更新日期：2005-07-15 00:00:00

  • Anterograde tracing of human hippocampus in vitro-a neuroanatomical tract tracing technique for the analysis of local fiber tracts in human brain.

    abstract：:Hippocampal slices were obtained from hippocampi of patients with temporal lobe epilepsy or from patients with mass lesions located in the temporal lobe. Hippocampal slices were kept alive in a slice chamber and the anterograde tracer neurobiotin was iontophoretically injected into the granule cell layer of the dentat...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/s0165-0270(02)00187-5

    authors： Freiman TM,Gimbel K,Honegger J,Volk B,Zentner J,Frotscher M,Deller T

    更新日期：2002-10-15 00:00:00

  • The interpretation of significance tests for independent and dependent samples.

    abstract：:The assumptions upon which a correct interpretation of the t-test depends are rarely fulfilled by data from the neurosciences. This applies to both independent and correlated samples. The Mann-Whitney U-test is suggested as an alternative for the t-test for independent samples. The way in which significant results fro...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章,评审

    doi：10.1016/0165-0270(83)90058-4

    authors： Krauth J

    更新日期：1983-12-01 00:00:00

  • A surgical technique of spinal cord cell transplantation in amyotrophic lateral sclerosis.

    abstract：:We report an original method for implanting bone marrow stem cells within the spinal cord parenchyma. This method was used for the experimental treatment of patients diagnosed with amyotrophic lateral sclerosis. The methodology is reproducible and devoid of major complications even in patients showing significant spin...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2010.06.014

    authors： Blanquer M,Pérez-Espejo MA,Martínez-Lage JF,Iniesta F,Martinez S,Moraleda JM

    更新日期：2010-08-30 00:00:00

  • Fast scanning and efficient photodetection in a simple two-photon microscope.

    abstract：:Two-photon laser scan microscopy carries many advantages for work on brain slices and bulk tissue. However, it has very low signal levels compared to conventional fluorescence microscopy. This is disadvantageous in fast imaging applications when photon shot noise is limiting. Working on brain slices with excitation po...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/s0165-0270(99)00103-x

    authors： Tan YP,Llano I,Hopt A,Würriehausen F,Neher E

    更新日期：1999-10-15 00:00:00

  • Development of BMP7-producing human cells, using a third generation lentiviral gene delivery system.

    abstract：:Bone morphogenetic protein 7 (BMP7), a member of the transforming growth factor β (TGF-β) superfamily, plays important roles in the development of various tissues and organs in mouse and human. In particular, BMP7 is critical for the formation of the nervous system and it is considered to have therapeutic potential in...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2011.12.007

    authors： Chitty DW,Tremblay RG,Ribecco-Lutkiewicz M,Haukenfrers J,Zurakowski B,Massie B,Sikorska M,Bani-Yaghoub M

    更新日期：2012-03-30 00:00:00