Abstract:
:Gelatinases are overexpressed in several types of maligancies and tumor stromal cells. Lidamycin is an enediyne antitumor antibiotic, which is composed of an apoprotein (LDP) and an active chromophore (AE). It is known that the heavy-chain complementarity-determining region-3 (CDR3) domain of scFv is important in antibody affinity. The aim of this study was to prepare the enediyne-energized fusion proteins with a heavy-chain CDR3 domain of anti-gelatinases scFv and lidamycin, and to evaluate their antitumor efficiency. Fusion proteins comprising the CDR3 domain and the lidamycin apoprotein were generated, and ELISA, immunofluorescence and FACS were used to analyze the binding of the fusion protein with antigen gelatinases. The purified fusion proteins were assembled with the lidamycin chromophore, and the antitumor effects were evaluated in vitro and in vivo. It was found that the CDR3-LDP and CDR3-LDP-CDR3 fusion proteins demonstrated high affinity towards antigen gelatinases. Following stimulation of CDR3-LDP with enediyne, the results of MTT showed potent cytotoxicity towards tumor cells; the IC50 values of CDR3-LDP-AE to HepG2 and Bel-7402 tumor cells were 1.05×10-11 and 6.6×10-14 M, respectively. In addition, CDR3-LDP-AE displayed a potent antitumor effect in H22 cell xenografts in mice; the combination of CDR3-LDP (10 mg/kg) and CDR3-LDP-AE (0.25 and 0.5 mg/kg) revealed that the tumor inhibitory rates were 85.2 and 92.7%, respectively (P<0.05 compared with CDR3-LDP-AE). In conclusion, these results suggest that the CDR3-LDP fusion protein and its analog CDR3-LDP-AE may both be promising candidates for tumor targeting therapy.
journal_name
Oncol Lettjournal_title
Oncology lettersauthors
Zhong GS,Wu MN,Guo XF,Xu ZS,Zhang SH,Zhen YSdoi
10.3892/ol.2013.1143subject
Has Abstractpub_date
2013-04-01 00:00:00pages
1183-1188issue
4eissn
1792-1074issn
1792-1082pii
ol-05-04-1183journal_volume
5pub_type
杂志文章相关文献
Oncology Letters文献大全abstract::The correlation between the expression levels of p65 and TNF-α in patients with acute myelocytic leukemia (AML) and AML cell lines were investigated. The bone marrow samples of 30 AML patients and 10 non-leukemia controls were studied. The mRNA expression levels of p65 and TNF-α were detected by reverse transcription-...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3720
更新日期:2015-11-01 00:00:00
abstract::Cluster of differentiation 44 (CD44), a marker for cancer stem cells, has been reported to be associated with poor prognosis in non-small cell lung cancer (NSCLC), but its involvement in tumor growth has not fully been elucidated. The present study explored the associations between CD44 expression and clinicopathologi...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8051
更新日期:2018-04-01 00:00:00
abstract::C-X-C chemokine receptor types 1/2 (CXCR1/2) may play multiple roles in the development and progression of a number of types of tumor. The abnormal expression of CXCR1/2 in various types of malignant tumors has been reported, but less is known with regard to gastric carcinoma. The present study was preliminarily condu...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.1043
更新日期:2013-02-01 00:00:00
abstract::Epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors (TKIs) are an approved first-line therapy against unresectable or advanced non-small cell lung cancer (NSCLC) harboring EGFR gene activating mutations. However, the majority of tumors develop acquired resistance against EGFR-TKIs and some tumors exhibi...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.11524
更新日期:2020-06-01 00:00:00
abstract::Peroxiredoxin 1 (Prx1) is important in the protection of cells from oxidative damage and the regulation of cell proliferation and apoptosis. Prx1 is overexpressed in oral precancerous lesions of oral leukoplakia (OLK) and oral cancer; however, the association between Prx1 expression and OLK pathogenesis remains unknow...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4659
更新日期:2016-07-01 00:00:00
abstract::Glutathione (GSH) is the keystone of the cellular response toward oxidative stress. Elevated GSH content correlates with increased resistance to chemotherapy and radiotherapy of head and neck (HN) tumors. The purpose of the present cross-sectional study was to evaluate whether the expression of glutamate-cysteine liga...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4458
更新日期:2016-06-01 00:00:00
abstract::The management of recurrent diffuse low-grade gliomas (LGGs) is controversial. In the present study, the multidisciplinary management of 35 patients with recurrent LGGs was retrospectively analyzed. Tumor progression or recurrence was defined by clinical, radiological and/or metabolic pejorative evolution. All patient...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6543
更新日期:2017-09-01 00:00:00
abstract::Hedgehog (HH) and Wnt pathway activation have been implicated in poor prognosis of breast cancer. Crosstalk between these two pathways has been demonstrated to be important in breast cancer progression, however the association between these two pathways and breast cancer survival rate is unknown. The present study com...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6874
更新日期:2017-11-01 00:00:00
abstract::The present study aimed to investigate the clinical characteristics, diagnosis and treatment of accessory breast cancer, and contribute valuable information regarding this rare tumour to the current literature, ultimately facilitating the development of improved treatment strategies. The present study reported the cas...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3388
更新日期:2015-09-01 00:00:00
abstract::Focal adhesion kinase (FAK) has long been considered to be a key regulator of growth factor receptor- and integrin-mediated signals, with pivotal roles in tumor cells through its kinase activity and scaffolding function. Increased FAK expression and activity has been observed in tumors of various origins and is often ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7676
更新日期:2018-03-01 00:00:00
abstract::Ascorbic acid (A) has been demonstrated to exhibit anti-cancer activity in association with chemotherapeutic agents. Potassium (K) is a regulator of cellular proliferation. In the present study, the biological effects of A and K bicarbonate, alone or in combination (A+K), on breast cancer cell lines were evaluated. Th...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4506
更新日期:2016-06-01 00:00:00
abstract::Notch-regulated ankyrin-repeat protein (NRARP) has recently been reported to be involved in a number of malignant cancers; however, its role in non-small lung cancer (NSCLC) remains unclear. The present study aimed to identify whether NRARP could be applied as a novel prognostic marker for NSCLC. A total of 108 NSCLC ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8826
更新日期:2018-08-01 00:00:00
abstract::The aim of the present study was to evaluate the curative effect of fludeoxyglucose-positron emission tomography/computed tomography (18F-FDG-PET/CT)-guided intensity-modulated radiotherapy (IMRT) for 42 patients with International Federation of Gynecology and Obstetrics (FIGO) stage III/IV ovarian cancer. Between Jan...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9601
更新日期:2019-01-01 00:00:00
abstract::Long non-coding RNAs (lncRNAs) are recently identified regulators in tumorigenesis and tumour progression. BRAF-activated lncRNA (BANCR) is overexpressed in melanoma and has a potential functional role in melanoma cell migration. However, little is known concerning the role of BANCR in the development of colorectal ca...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2154
更新日期:2014-08-01 00:00:00
abstract::Pulmonary inflammatory myofibroblastic tumors (PIMTs) are extremely rare in adults. If occurring in patients >40 years old, PIMT should be rapidly distinguished from lung cancer. The present study aimed to characterize the imaging features of PIMT in patients >40 years old in order to improve the diagnosis of PIMT. Th...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.2923
更新日期:2015-04-01 00:00:00
abstract::The expression of human transfer ribonucleic acid (tRNA) methyltransferase 9-like (KIAA1456) protein in lung cancer tissue and its effects on certain genes involved in the proliferation, migration and invasion of lung cancer cells were investigated. Immunohistochemistry was applied to stain lung cancer tissue and adja...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9119
更新日期:2018-09-01 00:00:00
abstract::Ovarian cancer is the most fatal gynecological cancer, with a 5-year survival rate of only 30%. Lysophosphatidic acid (LPA), which possesses growth factor-like functions, is a major regulatory factor in the peritoneal metastasis of ovarian cancer. LPA stimulates the expression of numerous genes that are associated wit...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.1926
更新日期:2014-05-01 00:00:00
abstract::Chylothorax is the accumulation of lymph fluid in the pleura. Gastric carcinoma with chylothorax and lymphedema as the initial manifestations has rarely been reported, with only 14 cases reported to date. The mechanisms of gastric carcinoma generating chylothorax have not yet been determined. The current study reports...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4285
更新日期:2016-04-01 00:00:00
abstract::Effects of different levels of PM2.5 on invasion and proliferation of HeLa cells and the expression levels of inflammatory cytokines IL-1 and IL-6 under 10 µg/ml PM2.5 were investigated. Different groups of HeLa cells were treated with PM2.5 at 0, 10, 25, 50, 100 and 200 µg/ml for 36 h, respectively. Cell proliferatio...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9516
更新日期:2018-12-01 00:00:00
abstract::The prognosis of patients with metastatic or recurrent breast cancer (MBC) is improving as novel treatments are developed. The present study compared the clinical characteristics of patients with MBC with or without a complete clinical response (cCR) and identified the survival-associated factors. This was a retrospec...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8145
更新日期:2018-05-01 00:00:00
abstract::Leiomyosarcomas account for ~24% of all adult sarcomas, and develop predominantly either in the uterus [uterine leiomyosarcoma (ULMS)] or in deep soft tissue or the retroperitoneum [non-uterine leiomyosarcoma (NULMS)]. Leiomyosarcomas are relatively chemoresistant tumors, and the prognosis of patients with leiomyosarc...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7274
更新日期:2018-01-01 00:00:00
abstract::Osteosarcoma (OS) is the third most frequent type of cancer in adolescents and represents >56% of all bone tumors. In addition, metastatic OS frequently demonstrates resistance to conventional chemotherapy; thus, the development of novel therapeutic agents for the treatment of patients with metastatic OS is warranted....
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.5953
更新日期:2017-06-01 00:00:00
abstract::Ovarian remnant syndrome (ORS) is a rare, but well-known gynecological complication, most often induced by difficult bilateral salpingo-oophorectomy (BSO) procedures that leave residual ovarian tissue on the pelvic wall. The most common preexisting conditions for this complication include endometriosis, pelvic inflamm...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2089
更新日期:2014-07-01 00:00:00
abstract::The present study investigated the value of combinations of five specific tumor biomarkers for the diagnosis of colorectal cancer (CRC): Neuron-specific enolase (NSE), carcinoembryonic antigen (CEA), cancer antigen (CA)19-9, CA125 and CA242. Associations between these markers and clinicopathological characteristics (i...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.11633
更新日期:2020-07-01 00:00:00
abstract::Non-steroidal nuclear receptors play a major role in breast cancer development. A correlation among, and possible prognostic function of, the members of the nuclear receptor superfamily has been discussed controversially over the years. Hence, we conducted a quantification of the different expression levels of the thy...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.799
更新日期:2012-10-01 00:00:00
abstract::The aim of this study was to investigate the correlation between cyclin A expression and efficacy of paclitaxel-based chemotherapy in patients with esophageal squamous cell carcinoma (ESCC). The expression of cyclin A was examined in 48 newly diagnosed ESCC patients prior to treatment using the MaxVision immunohistoch...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.814
更新日期:2012-10-01 00:00:00
abstract::The present study examined the expression of podocalyxin (PODX) in surgically-resected astrocytomas, associated the levels of PODX expression with the clinicopathological characteristics and survival outcomes of astrocytoma and assessed how PODX affected the viability of astrocytoma cells following the administration ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1556
更新日期:2013-11-01 00:00:00
abstract::Interleukin-8 (IL-8), an important inflammatory factor, is induced by Helicobacter pylori infection and is clearly associated with gastric cancer. IL-8 levels have been revealed to correlate significantly with the adhesion, migration and invasion of gastric cancer cells. However, whether IL-8 influences cell prolifera...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2531
更新日期:2014-12-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) can result from hepatitis B or C infection, fibrosis or cirrhosis. Transforming growth factor-β (TGF-β) is one of the main growth factors associated with fibrosis or cirrhosis progression in the liver, but its role is controversial in hepatocarcinogenesis. In the present study, the effec...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8441
更新日期:2018-06-01 00:00:00
abstract::Hypericum ascyron L. (Great St. Johnswort), which belongs to the Hypericaceae family, has been used for the treatment of hematemesis, metrorrhagia, rheumatism, swelling, stomach ache, abscesses, dysentery and irregular menstruation for >2,000 years in China. The aim of the present study was to clarify the anticancer a...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.7812
更新日期:2018-03-01 00:00:00