Cellular imaging and texture analysis distinguish differences in cellular dynamics in mouse brain tumors.

Abstract:

PURPOSE:The heterogeneous tumor cell population and dynamic microenvironment within a tumor lead to regional variations in cell proliferation, migration, and differentiation. In this work, MRI and optical projection tomography were used to examine and compare the redistribution of a cellular label in two mouse glioma models. METHODS:GL261 and 4C8 glioma cells labeled with iron oxide particles or with a fluorescent probe were injected into the brains of syngeneic mice and allowed to develop into ∼10-mm(3) tumors. Texture analysis was used to quantitatively describe and compare the label distribution patterns in the two tumor types. RESULTS:The label was seen to remain predominantly in the tumor core in GL261 tumors, but become more randomly distributed throughout the tumor volume in 4C8 tumors. Histologically, GL261 tumors displayed a more invasive, aggressive phenotype, although the distribution of mitotic cells in the two tumors was similar. CONCLUSION:The redistribution of a cellular label during tumor growth is characteristic of a tumor model. The label distribution map reflects more than simple differences in cell proliferation and is likely influenced by differences in the tumor microenvironment.

journal_name

Magn Reson Med

authors

Gazdzinski LM,Nieman BJ

doi

10.1002/mrm.24790

subject

Has Abstract

pub_date

2014-04-01 00:00:00

pages

1531-41

issue

4

eissn

0740-3194

issn

1522-2594

journal_volume

71

pub_type

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