Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil.


BACKGROUND:Histoplasmosis is a frequent fungal infection in HIV/AIDS patients, with high morbimortality rates when diagnosis and treatment are delayed. Antibody detection, which is faster than the gold standard culture test, hastens the laboratory investigation. OBJECTIVES:To evaluate the role of WB for antibody detection in the diagnosis of histoplasmosis among HIV/AIDS patients. PATIENTS AND METHODS:Fifty patients with proven or probable histoplasmosis were included. Clinical, epidemiological and laboratory data were described in the same population after a review of their medical records. WB was performed using deglycosylated histoplasmin. RESULTS:About 82% of patients were adult males and the mean age was 39.3 years. CD4+ T lymphocyte count less than 150 cells/mm3 was observed in 62% patients. Antibodies against Histoplasma capsulatum M antigen were detected in 62% of patients, and against both M and H antigens in 28% of individuals. Sera from 10% of patients were nonreactive. Histoplasmosis was the first opportunistic infection in 38% of the cases. Disseminated and pulmonary histoplasmosis occurred in 84% and 16% of patients, respectively. The overall mortality was 16%. CONCLUSION:WB could be useful for the histoplasmosis diagnosis in HIV/AIDS patients because of its easefulness and good sensitivity in a population where antibody production is hampered.






Almeida MA,Damasceno LS,Pizzini CV,Muniz MM,Almeida-Paes R,Zancopé-Oliveira RM




Has Abstract


2019-03-01 00:00:00














  • Genetic re-identification and antifungal susceptibility testing of Aspergillus section Nigri strains of the BCCM/IHEM collection.

    abstract::Black Aspergilli are widely distributed in the environment and are frequently reported as causative agents of different types of mycoses. Many taxonomical revisions have been made, and presently 19 different species are accepted. In this study we (re-) identified 123 strains of the Aspergillus niger group of the BCCM/...


    pub_type: 杂志文章


    authors: Hendrickx M,Beguin H,Detandt M

    更新日期:2012-03-01 00:00:00

  • [Therapy of intraabdominal fungal infections].

    abstract::Candida albicans is the fourth most germ that can be identified on surgical intensive care unit (SICU). During the course of severe peritonitis recognition of Candida is crucial for physicians but interpretation of Candida-positive microbiologic samples is difficult. The indication for antimycotic therapy requires dif...


    pub_type: 杂志文章,评审


    authors: Kujath P,Bouchard R,Nolde J,Scheele J

    更新日期:2005-01-01 00:00:00

  • Osteomyelitis due to Cryptococcus neoformans in advanced age. Case report and review of literature.

    abstract::In an 84-year-old man cryptococcosis with a pronounced osteomyelitic course (involvement of a rib, two thoracic vertebrae, the sacrum and a femur) was diagnosed histologically post mortem, thus directing attention to cryptococcosis in advanced age. The presumptive chronic course of the Cryptococcus neoformans (Cr. n.)...


    pub_type: 杂志文章,评审


    authors: Kromminga R,Staib F,Thalmann U,Jautzke G,Seibold M,Hochrein H,Witt H,Bürger H

    更新日期:1990-04-01 00:00:00

  • [Mycoses in patients with psoriasis or atopic dermatitis].

    abstract::Both, psoriasis and atopic dermatitis are multifactorial diseases with an unknown pathogenesis. To elucidate the influence of fungal infections in the onset or recurrence of those inflammatory skin disease we determined the frequencies of Candida and dermatophyte infections of the skin and orointestinal tract concomit...


    pub_type: 杂志文章


    authors: Henseler T,Tausch I

    更新日期:1997-01-01 00:00:00

  • Protective effect of human granulocyte colony-stimulating factor (hG-CSF) on Cryptococcus and Aspergillus infections in normal and immunosuppressed mice.

    abstract::Prophylactic treatment with human granulocyte colony-stimulating factor (hG-CSF) affords significant protection against systemic aspergillosis or pulmonary aspergillosis in neutropenic (cyclophosphamide-treated) mice but not in cortisone-treated animals. Cryptococcosis does not respond to hG-CSF therapy. Our data show...


    pub_type: 杂志文章


    authors: Polak-Wyss A

    更新日期:1991-05-01 00:00:00

  • Parameters for determination of Candida albicans virulence in murine peritonitis.

    abstract::Using intraperitoneal (i. p.) infection of mice with Candida albicans we determined which parameters might be useful for characterization of virulence in this model. Upon i. p. infection of mice with two reference strains striking differences in lethality were detected. These differences in virulence corresponded with...


    pub_type: 杂志文章


    authors: Kretschmar M,Bertsch T,Göller M,Schaller M,Hof H,Nichterlein T

    更新日期:1999-12-01 00:00:00

  • Evaluation of CHROMagar Candida for rapid screening of clinical specimens for Candida species.

    abstract::CHROMagar Candida is a new differential culture medium that allows selective isolation of yeasts and simultaneously identifies colonies of Candida albicans, Candida glabrata, Candida tropicalis and Candida krusei. We evaluated this medium and compared it with a reference medium, Sabouraud glucose agar, for the presump...


    pub_type: 杂志文章


    authors: Willinger B,Manafi M

    更新日期:1999-04-01 00:00:00

  • Genotyping of clinical and environmental Aspergillus flavus isolates from Iran using microsatellites.

    abstract::Aspergillus flavus is the second most important Aspergillus species causing human infections in tropical countries. Despite an increasing number of infections of A. flavus in Iran, the molecular epidemiology of clinical and environmental strains has not been well studied. We used a panel of nine microsatellite markers...


    pub_type: 杂志文章


    authors: Khodavaisy S,Badali H,Rezaie S,Nabili M,Moghadam KG,Afhami S,Hagen F,Aala F,Hashemi SJ,Meis JF

    更新日期:2016-04-01 00:00:00

  • A 7-year survey of dermatophytoses in Crete, Greece.

    abstract::Dermatophytoses are of worldwide distribution. Epidemiological studies concerning dermatophyte infections have been performed in many countries and differences in the incidence and the aetiological agents have been reported in different geographical locations. This study was undertaken to investigate the prevailing sp...


    pub_type: 杂志文章


    authors: Maraki S,Nioti E,Mantadakis E,Tselentis Y

    更新日期:2007-11-01 00:00:00

  • Comparison of 12 liquid media for germ tube production of Candida albicans and C. tropicalis.

    abstract::Infections caused by yeast of the genus Candida are the most common fungal infections, being Candida albicans the most common isolated species among them. The rapid identification of this yeast is mostly based on the production of germ tube in human or animal serum. This study describes the use of 12 different liquid ...


    pub_type: 杂志文章


    authors: Hilmioglu S,Ilkit M,Badak Z

    更新日期:2007-07-01 00:00:00

  • Inhibition of human pathogenic fungi by ethnobotanically selected plant extracts.

    abstract::In this study, 36 extracts derived from 29 plant species selected using an ethnobotanical approach were tested for antifungal activity against a taxonomically diverse group of 13 human pathogenic fungi. We compared the inhibitory characteristics of these plant extracts with those of the commonly used antifungals, amph...


    pub_type: 杂志文章


    authors: Ficker CE,Arnason JT,Vindas PS,Alvarez LP,Akpagana K,Gbéassor M,De Souza C,Smith ML

    更新日期:2003-02-01 00:00:00

  • Histoplasmosis: a review for clinicians from non-endemic areas.

    abstract::Histoplasmosis is an important systemic mycosis in the Americas, Asia, and Africa. Increasingly cases are recognised in nonendemic areas. Proper management requires recognition of the clinical syndromes caused by Histoplasma capsulatum infection, familiarity with the uses and limitations of the diagnostic tests, an un...


    pub_type: 杂志文章,评审


    authors: Wheat LJ

    更新日期:2006-07-01 00:00:00

  • Fluconazole susceptibility of Candida isolates from oropharyngeal candidosis.

    abstract::Fifty strains of Candida isolated from 38 patients with oropharyngeal candidosis were tested in vitro for fluconazole susceptibility with a disk diffusion test and for determination of minimal inhibitory concentrations (MICs). For 25 patients treated with fluconazole, the relationship between in vitro susceptibility a...


    pub_type: 杂志文章


    authors: Dannaoui E,Lacoste V,Prat C,Piens MA

    更新日期:1997-11-01 00:00:00

  • A clinical multicenter study comparing efficacy and tolerability of topical combination therapy with clotrimazole (Canesten, two formats) with oral single dose fluconazole (Diflucan) in vulvovaginal mycoses.

    abstract::Two topical formats containing clotrimazole [500 mg single dose vaginal tablet (VT) or 10% single dose vaginal cream (VC) for intravaginal use] combined with additional clotrimazole cream for topical application to the vulval area (Canesten 1 Combi, Bayer AG, Leverkusen, Germany) were compared with oral fluconazole 15...


    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Mendling W,Krauss C,Fladung B

    更新日期:2004-04-01 00:00:00

  • Fungicidal activity and morphological alterations of Candida albicans induced by echinocandins: study of strains with reduced caspofungin susceptibility.

    abstract::Caspofungin is a member of the echinocandin class of antifungal compounds that inhibit 1,3-β-d-Glucan synthase. As patient exposure to caspofungin (CAS) broadens, the number of infecting strains with reduced susceptibility to this drug is expected to rise. In the present study, the in vitro effects of varying concentr...


    pub_type: 杂志文章


    authors: Dunyach C,Drakulovski P,Bertout S,Jouvert S,Reynes J,Mallié M

    更新日期:2011-07-01 00:00:00

  • Burden of serious fungal infections in Belgium.

    abstract::We aimed to estimate the total number of serious fungal infections occurring yearly in Belgium. The number of cryptococcal infections was retrieved from the National Reference Center for Mycosis. Populations at risk and fungal infections frequencies in these populations were used to estimate incidence or prevalence of...


    pub_type: 杂志文章


    authors: Lagrou K,Maertens J,Van Even E,Denning DW

    更新日期:2015-10-01 00:00:00

  • Candida granuloma and sarcoidosis.

    abstract::Chronic mucocutaneous candidosis has been described in patients with impaired cell-mediated immunity. We describe a female patient with sarcoidosis who developed a Candida granuloma on her upper lip. Moderate impairment of the cell-mediated immunity was detected. Three months' treatment with ketoconazole was successfu...


    pub_type: 杂志文章


    authors: Mateev G,Vassileva S,Kantardjiev T,Obreshkova E,Pramatarov K

    更新日期:1993-09-01 00:00:00

  • Ocular tolerance of sertaconazole gel.

    abstract::The in vitro and in vivo tolerance of sertaconazole gel, a new topical azole antifungal, was studied. Ketoconazole gel (Panfungol) was used as a reference substance. The methods applied for tolerance assessment were the bovine corneal opacity and permeability test for the in vitro assay and a modified Draize test for ...


    pub_type: 杂志文章


    authors: Romero A,Grau MT,Villamayor F,Zapatero J,Mayordomo L,Tortajada A,Sacristán A,Ortiz JA

    更新日期:1996-01-01 00:00:00

  • EQUAL Aspergillosis Score 2018: An ECMM score derived from current guidelines to measure QUALity of the clinical management of invasive pulmonary aspergillosis.

    abstract::Invasive pulmonary aspergillosis is a serious threat to immunocompromised and critical care patients. Recent detailed guidelines and treatment algorithms lead microbiologists and clinicians in diagnosis and treatment of invasive aspergillosis. Currently, there is no tool available that allows to measure guideline adhe...


    pub_type: 杂志文章


    authors: Cornely OA,Koehler P,Arenz D,C Mellinghoff S

    更新日期:2018-11-01 00:00:00

  • Effect of reducing agents on proteolytic and keratinolytic activity of enzymes of Microsporum gypseum.

    abstract::The effect of sodium sulphite, cysteine, glutathione, mercaptoethanol and dithioerythritol (0.1-10 mmol l-1) on the activity of proteases of Microsporum gypseum was studied using azocasein, cross-linked bovine serum albumin and keratin as substrates. With the substrate without disulphide bonds (casein) no stimulation ...


    pub_type: 杂志文章


    authors: Kunert J

    更新日期:1992-11-01 00:00:00

  • Unilateral renal phaeohyphomycosis due to Bipolaris spicifera in an immunocompetent child - rare case presentation and review of literature.

    abstract::Phaeohyphomycosis refers to infections caused by phaeoid fungi that can have an aggressive course in normal hosts. We report a case of left-sided renal phaeohyphomycosis due to Bipolaris spicifera in a 7-year-old immunocompetent male child. He presented with fever, dysuria, nausea, vomiting and flank pain. Examination...


    pub_type: 杂志文章,评审


    authors: Kaur R,Bala K

    更新日期:2015-07-01 00:00:00

  • Aetiology of onychomycosis in Al Ain, United Arab Emirates.

    abstract::A 1-year study was conducted on 151 patients with finger- or toenail disease attending a dermatology clinic in Al Ain, United Arab Emirates. Nail scrapings and or clippings were collected and investigated with a KOH preparation for direct microscopy and cultured for fungi. Eighty-nine (59%) patients were females. Most...


    pub_type: 杂志文章


    authors: Nsanze H,Lestringant GG,Mustafa N,Usmani MA

    更新日期:1995-09-01 00:00:00

  • Effects of recombinant human granulocyte-colony stimulating factor on neutropenic mice infected with Candida albicans: acceleration of recovery from neutropenia and potentiation of anti-C. albicans resistance.

    abstract::The treatment of systemic candidal infection in neutropenic patients continues to be a major problem, and only 20% of patients survive despite treatment with amphotericin B (Amph B). Granulocyte colony-stimulating factor (G-CSF) is a haemopoietic glycoprotein that appears to control the survival, cycle, activation, pr...


    pub_type: 杂志文章


    authors: Hamood M,Bluche PF,De Vroey C,Corazza F,Bujan W,Fondu P

    更新日期:1994-03-01 00:00:00

  • Case report. Fatal Aspergillus flavus pericarditis in a patient with acute myeloblastic leukaemia.

    abstract::We report a case of Aspergillus flavus pericarditis treated with fluconazole for oral candidosis. The patient with acute myeloblastic leukaemia developed tachypnoea after antileukaemic chemotherapy. Pericardial effusion was seen in the echocardiogram. Aspergillus flavus was isolated from the pericardial fluid. The pat...


    pub_type: 信件


    authors: Gökahmetoğlu S,Koç AN,Patiroğlu T

    更新日期:2000-01-01 00:00:00

  • [Differentiation and characterization of yeasts pathogenic for humans (Candida albicans, Exophiala dermatitidis) and algae pathogenic for animals (Prototheca spp.) using Fourier transform infrared spectroscopy (FTIR) in comparison with conventional method

    abstract::Due to the Fourier-Transform Infrared Spectroscopy (FT-IR) of strain specific traits demonstrated to be a suitable and efficient method for diagnostic and epidemiological determinations for the yeasts Candida albicans, Exophiala dermatitidis and the chlorophylless algae of the genus Prototheca. FT-IR leads in a rapid ...


    pub_type: 杂志文章


    authors: Schmalreck AF,Tränkle P,Vanca E,Blaschke-Hellmessen R

    更新日期:1998-01-01 00:00:00

  • Adherence of Candida albicans and Candida parapsilosis to epithelial cells correlates with fungal cell surface carbohydrates.

    abstract::Many studies have described the adherence of Candida albicans to epithelial cells but little is known about Candida parapsilosis adhesion and its role in host cell surface recognition. This study was designed to evaluate the correlation between the adherence of 20 C. albicans and 12 C. parapsilosis strains to human bu...


    pub_type: 杂志文章


    authors: Lima-Neto RG,Beltrão EI,Oliveira PC,Neves RP

    更新日期:2011-01-01 00:00:00

  • Isolation of Cryptococcus neoformans from pigeon (Columba livia) droppings in Mexico City.

    abstract::To determine the ecological and epidemiological significance of pigeon excrement in cryptococcosis in Mexico City, 251 samples of pigeon droppings were studied. These were collected from houses, public buildings, churches, parks and pigeon nests. Each sample was suspended 1:10 in isotonic saline solution and then cult...


    pub_type: 杂志文章


    authors: Castanón-Olivares LR,López-Martínez R

    更新日期:1994-09-01 00:00:00

  • Dermatophytes and other fungi associated with skin mycoses in Tripoli, Libya.

    abstract::This study sought to determine the prevalence of skin infections and their causative agents in the Libyan population. Samples were collected from 2224 patients attending the Dermatology Clinics of the Tripoli Medical Centre (TMC) between August 1997 and December 1999 and were submitted to a mycology laboratory for ana...


    pub_type: 杂志文章


    authors: Ellabib MS,Khalifa Z,Kavanagh K

    更新日期:2002-04-01 00:00:00

  • The design and application of an automated microscope developed based on deep learning for fungal detection in dermatology.

    abstract:BACKGROUND:Light microscopy to study the infection of fungi in skin specimens is time-consuming and requires automation. OBJECTIVE:We aimed to design and explore the application of an automated microscope for fungal detection in skin specimens. METHODS:An automated microscope was designed, and a deep learning model w...


    pub_type: 杂志文章


    authors: Gao W,Li M,Wu R,Du W,Zhang S,Yin S,Chen Z,Huang H

    更新日期:2020-11-10 00:00:00

  • [Candida identification: experiences with the Vitek automated system].

    abstract::We report about our experiences obtained with the Vitek system (bioMerieux, Nürtingen) for identification of 1160 clinical Candida isolates. The Vitek system correctly identified 1005 (86.6%) Candida isolates (probability > or = 85%): 776 (66.9%) strains were identified after 24 h of incubation, 229 (19.7%) required 4...


    pub_type: 杂志文章


    authors: Schulz K,Bernhardt H,Schröder G

    更新日期:1997-01-01 00:00:00