Comparative stability, sensitivity and specificity studies with different lots of Blastomyces dermatitidis yeast and mycelial lysate antigens.

Abstract:

:Comparative studies were performed to assess the stability and lot-to-lot variation of Blastomyces dermatitidis yeast and mycelial phase lysate antigens. Four lots were prepared from each growth phase of B. dermatitidis strain T-58 (canine isolate) during a 14-month period. Serum specimens from dogs with blastomycosis, histoplasmosis, coccidioidomycosis, cryptococcosis and aspergillosis were assayed for antibody content using an alkaline phosphatase enzyme-linked immunosorbent assay (ELISA). The four lots of the yeast phase reagents were similar with respect to sensitivity and specificity, and the absorbance readings were approximately four times greater with sera from dogs with blastomycosis than with histoplasmosis or coccidioidomycosis. Even less cross-reactivity was evidenced when the sera from dogs with cryptococcosis and aspergillosis were assayed. In contrast, the four lots of the mycelial lysate reagents were considerably less reactive and more cross-reactive than the yeast phase antigens and, as above, the four reagents retained their activity after prolonged storage. Therefore the results indicated that the lysate antigens exhibited a great deal of stability and lot-to-lot variations in activity were not observed.

journal_name

Mycoses

journal_title

Mycoses

authors

Orr ML,Bono JL,Abuodeh RO,Williams SJ,Legendre AM,Scalarone GM

doi

10.1111/j.1439-0507.1994.tb00293.x

subject

Has Abstract

pub_date

1994-06-01 00:00:00

pages

155-60

issue

5-6

eissn

0933-7407

issn

1439-0507

journal_volume

37

pub_type

杂志文章

相关文献

MYCOSES文献大全
  • [Therapy of intraabdominal fungal infections].

    abstract::Candida albicans is the fourth most germ that can be identified on surgical intensive care unit (SICU). During the course of severe peritonitis recognition of Candida is crucial for physicians but interpretation of Candida-positive microbiologic samples is difficult. The indication for antimycotic therapy requires dif...

    journal_title:Mycoses

    pub_type: 杂志文章,评审

    doi:10.1111/j.1439-0507.2005.01115.x

    authors: Kujath P,Bouchard R,Nolde J,Scheele J

    更新日期:2005-01-01 00:00:00

  • Isolation of Cryptococcus neoformans from pigeon (Columba livia) droppings in Mexico City.

    abstract::To determine the ecological and epidemiological significance of pigeon excrement in cryptococcosis in Mexico City, 251 samples of pigeon droppings were studied. These were collected from houses, public buildings, churches, parks and pigeon nests. Each sample was suspended 1:10 in isotonic saline solution and then cult...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.1994.37.9-10.325

    authors: Castanón-Olivares LR,López-Martínez R

    更新日期:1994-09-01 00:00:00

  • Invasive aspergillosis with polyarthritis.

    abstract::We report a case of septic arthritis of multiple joints in an 18-year-old male caused by Aspergillus fumigatus. His initial presentation was of low-grade fever followed by involvement of both knee and ankle joints. Later, there was also involvement of metacarpophalangeal joints. Diagnosis was based on biopsy and cultu...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2004.01031.x

    authors: Mekan SF,Saeed O,Khan JA

    更新日期:2004-12-01 00:00:00

  • Screening examination and treatment of Trichophyton tonsurans infection in judo athletes affiliated with the University Judo Federation of Tokyo.

    abstract::In Japan, Trichophyton tonsurans infection has become an increasing problem among combat sports participants. We investigated the prevalence of T. tonsurans infection in athletes affiliated to judo clubs in the 21 First Division universities that were registered with the University Judo Federation of Tokyo in 2008. St...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2009.01823.x

    authors: Hirose N,Suganami M,Ogawa YS,Hiruma M,Ogawa H

    更新日期:2011-07-01 00:00:00

  • Anti-Cryptococcus activity of combination of extracts of Cassia alata and Ocimum sanctum.

    abstract::The paper reports the anti-Cryptococcus activity of combination of ethanolic extracts of leaves of Cassia alata and Ocimum sanctum. The activity of combination of the extracts was heat-stable and worked at acidic pH. ...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1046/j.1439-0507.2000.00581.x

    authors: Ranganathan S,Balajee SA

    更新日期:2000-09-01 00:00:00

  • [Itraconazole suspension in the treatment of HIV-infected patients with fluconazole-resistant oropharyngeal candidiasis and esophagitis].

    abstract::Forty AIDS patients suffering from fluconazole-resistant oropharyngeal candidosis were treated with oral itraconazole solution (200-800 mg/day). In 39/49 patients the disease could be initially cured or significantly improved. In contrast to the good clinical results, however, only in six patients a yeast count reduct...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1996.tb00514.x

    authors: Eichel M,Just-Nübling G,Helm EB,Stille W

    更新日期:1996-01-01 00:00:00

  • Ecthyma-like phaeohyphomycosis caused by Cladosporium cladosporioides.

    abstract::A case of cutaneous phaeohyphomycosis caused by Cladosporium cladosporioides in a 50-year-old housewife is described. The clinical presentation was an ecthyma-like crusted lesion on the back of her left hand. Scanning electron microscopy of the culture showed the conidiophores and the limoniform or ellipsoidal conidia...

    journal_title:Mycoses

    pub_type: 信件

    doi:10.1111/j.1439-0507.2009.01745.x

    authors: Duquia RP,de Almeida HL Jr,Vettorato G,Rocha NM,de Castro LA

    更新日期:2010-11-01 00:00:00

  • In vitro antifungal susceptibility of clinical isolates of Candida spp. from hospitalized patients.

    abstract::A total of 122 Candida spp. strains, isolated from a group of 100 patients hospitalized in the Santa Casa de Misericordia of Belo Horizonte were assayed for in vitro susceptibility to amphotericin B, fluconazole, itraconazole, ketoconazole and flucytosine using a microbroth technique proposed by the National Committee...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1046/j.1439-0507.1999.00522.x

    authors: de Resende JC,de Resende MA

    更新日期:1999-01-01 00:00:00

  • Trichophyton rubrum suppurative tinea of the bald area of the scalp.

    abstract::An 83-year-old man presented with an approximately 1-year history of an extensive inflammatory purulent crusted lesion in the bald area of the scalp diagnosed as tinea caused by Trichophyton rubrum. The scalp biopsy specimen showed suppurative folliculitis with perifollicular abscesses in upper dermis, and periodic ac...

    journal_title:Mycoses

    pub_type: 信件

    doi:10.1111/j.1439-0507.2009.01768.x

    authors: Kuklová I,Stork J,Lacina L

    更新日期:2011-01-01 00:00:00

  • Inhibitory effect of terbinafine on reactive oxygen species (ROS) generation by Candida albicans.

    abstract::Candida albicans, the most important opportunistic fungal pathogen, is able to generate remarkable amounts of reactive oxygen species (ROS). Since ROS are highly cytotoxic, this mechanism may contribute to the pathogenicity of this yeast, including its invasiveness and the inflammatory response of the host. Terbinafin...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1046/j.1439-0507.2002.00756.x

    authors: Sander CS,Hipler UC,Wollina U,Elsner P

    更新日期:2002-06-01 00:00:00

  • Fungicidal activity and morphological alterations of Candida albicans induced by echinocandins: study of strains with reduced caspofungin susceptibility.

    abstract::Caspofungin is a member of the echinocandin class of antifungal compounds that inhibit 1,3-β-d-Glucan synthase. As patient exposure to caspofungin (CAS) broadens, the number of infecting strains with reduced susceptibility to this drug is expected to rise. In the present study, the in vitro effects of varying concentr...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2009.01834.x

    authors: Dunyach C,Drakulovski P,Bertout S,Jouvert S,Reynes J,Mallié M

    更新日期:2011-07-01 00:00:00

  • Case of dermatophyte abscess caused by Trichophyton rubrum: a case report and review of the literature.

    abstract::A 54-year-old Japanese man without apparent immunosuppression presented with nodules with purulent drainage on the right lower leg. He had ringworm of the right leg and tinea unguium. A biopsy specimen of the nodule showed intradermal abscesses with fungal elements, and Trichophyton rubrum was cultured from both the p...

    journal_title:Mycoses

    pub_type: 杂志文章,评审

    doi:10.1111/myc.12317

    authors: Inaoki M,Nishijima C,Miyake M,Asaka T,Hasegawa Y,Anzawa K,Mochizuki T

    更新日期:2015-05-01 00:00:00

  • Invasive cutaneous infection caused by Apophysomyces elegans associated with a spider bite.

    abstract::Cutaneous infections by Zygomycetes may have underestimated clinical consequences. Apophysomyces elegans is a Zygomycete that rarely causes disease in humans. However, it has been reported with increasing frequency in warm climate zones as a result of infection in healthy patients after injury to the cutaneous barrier...

    journal_title:Mycoses

    pub_type: 信件

    doi:10.1111/j.1439-0507.2009.01698.x

    authors: Saravia-Flores M,Guaran DM,Argueta V

    更新日期:2010-05-01 00:00:00

  • Scutular tinea of the scrotum: report of two cases.

    abstract::Infections caused by dermathophytes in the scrotal skin are uncommon especially due to Microsporum gypseum, which may form scutular or favus-like lesions. We report two patients with this type of tinea: one immune suppressed by HIV infection and another immunocompetent without comorbidity. In the literature we found o...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2005.01097.x

    authors: Prochnau A,de Almeida HL Jr,Souza PR,Vetoratto G,Duquia RP,Defferrari R

    更新日期:2005-05-01 00:00:00

  • A novel dimorphic pathogen, Emergomyces orientalis (Onygenales), agent of disseminated infection.

    abstract::A novel dimorphic fungus, Emergomyces orientalis sp. nov. a close relative of systemic pathogens in the family Ajellomycetaceae (Blastomyces, Histoplasma). The fungus is reported in a 64-year-old male from Shanxi, China. The patient developed disseminated skin lesions, productive cough with fever and showed nodular op...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12583

    authors: Wang P,Kenyon C,de Hoog S,Guo L,Fan H,Liu H,Li Z,Sheng R,Yang Y,Jiang Y,Zhang L,Xu Y

    更新日期:2017-05-01 00:00:00

  • Comparative evaluation of galactomannan optical density indices and culture results in bronchoscopic specimens obtained from neutropenic and non-neutropenic patients.

    abstract::Aspergillus infections are major causes of morbidity and mortality among immunocompromised patients. This study was designed to investigate the galactomannan assay optical density (OD) indices relative to the culture results in bronchoscopic samples obtained from neutropenic and non-neutropenic patients. Galactomannan...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12126

    authors: Ağca H,Ener B,Yılmaz E,Ursavaş A,Kazak E,Özkocaman V,Çetinoğlu ED,Dilektaşlı AG,Akalın H,Özkalemkaş F,Ali R

    更新日期:2014-03-01 00:00:00

  • Case reports. Disseminated cryptococcoses without cryptococcal antigen detection.

    abstract::During the last decade cryptococcoses were most frequently diagnosed in AIDS patients, where serologically high amounts of glucoronoxylomannan (GXM) were detectable. Disseminated cryptococcoses without cryptococcal antigen detection is unusual. Between August and October 1998 disseminated cryptococcoses were diagnosed...

    journal_title:Mycoses

    pub_type: 信件

    doi:10.1046/j.1439-0507.2000.00556.x

    authors: Tintelnot K,Adler S,Bergmann F,Schönherr K,Seibold M

    更新日期:2000-01-01 00:00:00

  • RAPD differentiation of Malassezia spp. from cattle, dogs and humans.

    abstract::The molecular characterization of Malassezia spp. isolates from animals and humans has not been thoroughly studied. We have analysed the DNA profile by random amplified polymorphic DNA (RAPD)-PCR to compare the genetic diversity between isolates from the external ears of cattle, dogs and humans. The analysis of electr...

    journal_title:Mycoses

    pub_type: 杂志文章,评审

    doi:10.1111/j.1439-0507.2008.01658.x

    authors: Duarte ER,Hamdan JS

    更新日期:2010-01-01 00:00:00

  • Inhibition of dermatophytes by optical brighteners.

    abstract::The aim of this study was to investigate a possible effect of optical brighteners on the growth of dermatophytes. Typical strains of Trichophyton rubrum, T. mentagrophytes, Microsporum canis and Epidermophyton floccosum were grown on agar plates containing two different brighteners of stilbenedisulfonic acid type in c...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1046/j.1439-0507.2003.00857.x

    authors: Brasch J,Kreiselmaier I,Christophers E

    更新日期:2003-04-01 00:00:00

  • Ocular tolerance of sertaconazole gel.

    abstract::The in vitro and in vivo tolerance of sertaconazole gel, a new topical azole antifungal, was studied. Ketoconazole gel (Panfungol) was used as a reference substance. The methods applied for tolerance assessment were the bovine corneal opacity and permeability test for the in vitro assay and a modified Draize test for ...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1996.tb00085.x

    authors: Romero A,Grau MT,Villamayor F,Zapatero J,Mayordomo L,Tortajada A,Sacristán A,Ortiz JA

    更新日期:1996-01-01 00:00:00

  • Preparation of antigens from Trichophyton mentagrophytes using a new semi-solid culture medium and their characterization by SDS-PAGE and immunological techniques.

    abstract::A new semi-solid culture medium was developed by substituting the agar in Sabouraud glucose medium by Lutrol FC 127 (BASF, Ludwigshafen, Germany). This culture medium can be liquefied by cooling it down from the incubation temperature to 5-10 degrees C, thus allowing the full harvest of fungal mycelium without any con...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1992.tb00893.x

    authors: Garg AP,Müller J

    更新日期:1992-11-01 00:00:00

  • Treatment of human cutaneous sporotrichosis with itraconazole.

    abstract::Eighteen adult white male patients with cutaneous sporotrichosis were treated with itraconazole following different daily dose schemes. Cure was obtained in all cases after periods of 15-75 days (median 44 days) with total doses between 3.1 and 14.8 g (median 8.4 g). No serious side effects were observed and no relaps...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1992.tb00836.x

    authors: Conti Díaz IA,Civila E,Gezuele E,Lowinger M,Calegari L,Sanabria D,Fuentes L,Da Rosa D,Alzueta G

    更新日期:1992-05-01 00:00:00

  • Comparative studies on the detection of antibodies and delayed hypersensitivity responses with 10 Blastomyces dermatitidis lysate antigens.

    abstract::Yeast-phase lysate antigens were prepared from 10 different isolates of Blastomyces dermatitidis. Comparative studies were performed using the lysate antigens in an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in sera from dogs with blastomycosis and histoplasmosis. In order to evaluate th...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1997.tb00206.x

    authors: Wakamoto A,Abuodeh RO,Scalarone GM

    更新日期:1997-10-01 00:00:00

  • Effects of repeated low-dose UVB irradiation on the hyphal growth of Candida albicans.

    abstract::Ultraviolet B light (UVB) can have negative phototropic effects on fungi. Candida albicans is often found on human skin exposed to UVB. Therefore, it is of medical interest to know whether a negative phototropic response to UVB irradiation can support an invasive growth of this potentially dangerous agent. In our stud...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2005.01169.x

    authors: Brasch J,Kay C

    更新日期:2006-01-01 00:00:00

  • Antifungal activity of posaconazole and granulocyte colony-stimulating factor in the treatment of disseminated zygomycosis (mucormycosis) in a neutropaenic murine model.

    abstract::The aim of this study was to evaluate the pharmacokinetics and efficacy of posaconazole (PSC) in combination with granulocyte colony-stimulating factor (G-CSF) in a neutropaenic murine model of disseminated zygomycosis (mucormycosis) due to Rhizopus microsporus. Male BALB/c mice were rendered neutropaenic with cycloph...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2010.01958.x

    authors: Saoulidis S,Simitsopoulou M,Dalakiouridou M,Walsh TJ,Wheat LJ,Papaioannidou P,Roilides E

    更新日期:2011-09-01 00:00:00

  • Clinical and pharmacoeconomic evaluation of antifungal prophylaxis with continuous micafungin in patients undergoing allogeneic stem cell transplantation: A six-year cohort analysis.

    abstract:BACKGROUND:Patients undergoing allogeneic stem cell transplantation (aSCT) are at high risk to develop an invasive fungal disease (IFD). Optimisation of antifungal prophylaxis strategies may improve patient outcomes and reduce treatment costs. OBJECTIVES:To analyse the clinical and economical impact of using continuou...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.13232

    authors: Wingen-Heimann SM,Cornely OA,J G T Vehreschild M,Wisplinghoff H,Franke B,Schons M,von Bergwelt-Baildon M,Scheid C,Vehreschild JJ

    更新日期:2020-12-22 00:00:00

  • Antifungal susceptibility and genotypes of Candida albicans strains from patients with vulvovaginal candidiasis.

    abstract::Studies of the genetic diversity of Candida albicans strains and the correlation between the antifungal susceptibility and gene diversity of C. albicans were carried out and the results were found to be inconsistent. To investigate antifungal susceptibility and genotypes of C. albicans strains from patients with vulvo...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2008.01539.x

    authors: Liu XP,Fan SR,Bai FY,Li J,Liao QP

    更新日期:2009-01-01 00:00:00

  • High-dose posaconazole for azole-resistant aspergillosis and other difficult-to-treat mould infections.

    abstract:BACKGROUND:Oral follow-up therapy is problematic in moulds with reduced azole-susceptibility, such as azole-resistant Aspergillus fumigatus infection. Currently, only intravenous liposomal amphotericin B (L-AmB) is advocated by guidelines for the treatment of azole-resistant aspergillosis infections. Preclinical resear...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.13028

    authors: Schauwvlieghe AFAD,Buil JB,Verweij PE,Hoek RAS,Cornelissen JJ,Blijlevens NMA,Henriet SSV,Rijnders BJA,Brüggemann RJM

    更新日期:2020-02-01 00:00:00

  • Production of hybrid phage displaying secreted aspartyl proteinase epitope of Candida albicans and its application for the diagnosis of disseminated candidiasis.

    abstract::The secreted aspartyl proteinases (Saps) of Candida albicans have been implicated as immunodominant antigens and virulence factors associated with adherence and tissue invasion. A hybrid phage displaying the Sap epitope VKYTS was constructed by cloning the corresponding DNA fragments into the pfd88 vector. Similar to ...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2006.01349.x

    authors: Yang Q,Su QP,Wang GY,Wen DZ,Zhang YH,Bao HZ,Wang L

    更新日期:2007-05-01 00:00:00

  • Glycoconjugates and polysaccharides from the Scedosporium/Pseudallescheria boydii complex: structural characterisation, involvement in cell differentiation, cell recognition and virulence.

    abstract::Peptidorhamnomannans (PRMs), rhamnomannans and α-glucans are especially relevant for the architecture of the Scedosporium/Pseudallescheria boydii cell wall, but many of them are immunologically active, with great potential as regulators of pathogenesis and the immune response of the host. In addition, some of them can...

    journal_title:Mycoses

    pub_type: 杂志文章,评审

    doi:10.1111/j.1439-0507.2011.02105.x

    authors: Lopes LC,da Silva MI,Bittencourt VC,Figueiredo RT,Rollin-Pinheiro R,Sassaki GL,Bozza MT,Gorin PA,Barreto-Bergter E

    更新日期:2011-10-01 00:00:00