Production of hybrid phage displaying secreted aspartyl proteinase epitope of Candida albicans and its application for the diagnosis of disseminated candidiasis.

Abstract:

:The secreted aspartyl proteinases (Saps) of Candida albicans have been implicated as immunodominant antigens and virulence factors associated with adherence and tissue invasion. A hybrid phage displaying the Sap epitope VKYTS was constructed by cloning the corresponding DNA fragments into the pfd88 vector. Similar to native Sap, the phage-displayed epitope showed reactivity to sera from mice and patients with systemic C. albicans infection but not from those with oropharyngeal candidiasis and healthy individuals on Western blot. Furthermore, a new enzyme-linked immunosorbent assay was developed to detect the anti-Sap antibody with hybrid phage displaying Sap epitope VKYTS that can be recognised by anti-Sap antibodies. Sequential sera were tested from patients and mice with systemic candidiasis and oropharyngeal candidiasis, and serum samples from healthy individuals were also included. The sensitivity and specificity were 77% and 88.3% for experimental mice, respectively. These values reached 60% and 85%, respectively, for human patients. These data indicate this phage-displayed epitope as an effective and less expensive reagent would be a valuable probe for the detection of specific Sap antibody in the sera of patients and mice with systemic C. albicans infection.

journal_name

Mycoses

journal_title

Mycoses

authors

Yang Q,Su QP,Wang GY,Wen DZ,Zhang YH,Bao HZ,Wang L

doi

10.1111/j.1439-0507.2006.01349.x

subject

Has Abstract

pub_date

2007-05-01 00:00:00

pages

165-71

issue

3

eissn

0933-7407

issn

1439-0507

pii

MYC1349

journal_volume

50

pub_type

杂志文章

相关文献

MYCOSES文献大全
  • Aetiology of onychomycosis in Al Ain, United Arab Emirates.

    abstract::A 1-year study was conducted on 151 patients with finger- or toenail disease attending a dermatology clinic in Al Ain, United Arab Emirates. Nail scrapings and or clippings were collected and investigated with a KOH preparation for direct microscopy and cultured for fungi. Eighty-nine (59%) patients were females. Most...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1995.tb00075.x

    authors: Nsanze H,Lestringant GG,Mustafa N,Usmani MA

    更新日期:1995-09-01 00:00:00

  • [Mycoses in patients with psoriasis or atopic dermatitis].

    abstract::Both, psoriasis and atopic dermatitis are multifactorial diseases with an unknown pathogenesis. To elucidate the influence of fungal infections in the onset or recurrence of those inflammatory skin disease we determined the frequencies of Candida and dermatophyte infections of the skin and orointestinal tract concomit...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1997.tb00537.x

    authors: Henseler T,Tausch I

    更新日期:1997-01-01 00:00:00

  • Our recommendations for avoiding exposure to fungi outside the hospital for patients with haematological cancers.

    abstract::Despite several chemotherapeutic and preventative advances, opportunistic fungal infections remain common unintended consequences of cancer treatment. Currently, cancer patients spend most of their time between treatments at home, where they can inadvertently come across potential hazards from environmental and food s...

    journal_title:Mycoses

    pub_type: 杂志文章,评审

    doi:10.1111/myc.12167

    authors: Ariza-Heredia EJ,Kontoyiannis DP

    更新日期:2014-06-01 00:00:00

  • Effects of recombinant human granulocyte-colony stimulating factor on neutropenic mice infected with Candida albicans: acceleration of recovery from neutropenia and potentiation of anti-C. albicans resistance.

    abstract::The treatment of systemic candidal infection in neutropenic patients continues to be a major problem, and only 20% of patients survive despite treatment with amphotericin B (Amph B). Granulocyte colony-stimulating factor (G-CSF) is a haemopoietic glycoprotein that appears to control the survival, cycle, activation, pr...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1994.tb00783.x

    authors: Hamood M,Bluche PF,De Vroey C,Corazza F,Bujan W,Fondu P

    更新日期:1994-03-01 00:00:00

  • Candiduria in haematologic malignancy patients without a urinary catheter: nothing more than a frailty marker?

    abstract::There is scarcity of data regarding significance of candiduria in patients with haematologic malignancies and its association with invasive candidiasis. To that end, we retrospectively evaluated all hospitalised, non-intensive care unit patients with haematologic malignancies and candiduria during a 10-year period (20...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12024

    authors: Georgiadou SP,Tarrand J,Sipsas NV,Kontoyiannis DP

    更新日期:2013-05-01 00:00:00

  • [Molecular biological methods and their consequences in taxonomy and diagnosis of dermatophytes].

    abstract::Trichophyton rubrum, T. mentagrophytes, T. verrucosum, Microsporum canis, M. gypseum and Epidermophyton flocoosum represent the cause of human dermatomycoses isolated most often at the University Hospital of Dermatology in Graz, Austria, between 1991 and 1998. So far, identification was mainly based on the cultivation...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:

    authors: Blanz P,Buzina W,Ginter G,Gräser Y

    更新日期:2000-01-01 00:00:00

  • Fluconazole monitoring in Candida peritonitis based on histological control.

    abstract::Between May 1, 1988 and January 1, 1990, 10 patients were treated for Candida peritonitis. Origins of the infections were lesions and perforations of the gastrointestinal tract. Risk factors promoting the disease were tumours, diabetes mellitus and extensive antibiotic therapy. The mean classification by the APACHE sc...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.1990.33.9-10.441

    authors: Kujath P,Lerch K,Dämmrich J

    更新日期:1990-09-01 00:00:00

  • Usefulness of serum galactomannan in initiating and modifying antifungal therapy in children with cancer and persistent high-risk febrile neutropenia.

    abstract:BACKGROUND:Invasive fungal disease is a major cause of morbidity and mortality in children with cancer and high-risk febrile neutropenia (HRFN). Repeated serum galactomannan (sGM) measurements have been described as an effective tool to guide therapy in adults under suspicion of invasive aspergillosis. However, the uti...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.13097

    authors: Delgado-Araneda M,Valenzuela R,de la Maza V,Rabello M,Álvarez AM,Contardo V,Zubieta M,Gutierrez V,Claverie X,Torres JP,Salgado C,Tordecilla J,Varas M,Avilés CL,Venegas M,Villarroel M,Santolaya ME

    更新日期:2020-08-01 00:00:00

  • Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil.

    abstract:BACKGROUND:Histoplasmosis is a frequent fungal infection in HIV/AIDS patients, with high morbimortality rates when diagnosis and treatment are delayed. Antibody detection, which is faster than the gold standard culture test, hastens the laboratory investigation. OBJECTIVES:To evaluate the role of WB for antibody detec...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12877

    authors: Almeida MA,Damasceno LS,Pizzini CV,Muniz MM,Almeida-Paes R,Zancopé-Oliveira RM

    更新日期:2019-03-01 00:00:00

  • Genetic diversity and antifungal susceptibility of Fusarium isolates in onychomycosis.

    abstract::Fusarium species have emerged as an important human pathogen in skin disease, onychomycosis, keratitis and invasive disease. Onychomycosis caused by Fusarium spp. The infection has been increasingly described in the immunocompetent and immunosuppressed hosts. Considering onychomycosis is a difficult to treat infection...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12638

    authors: Rosa PD,Heidrich D,Corrêa C,Scroferneker ML,Vettorato G,Fuentefria AM,Goldani LZ

    更新日期:2017-09-01 00:00:00

  • Mycological survey of activated sludge in MBRs.

    abstract::The objective of this research was to conduct a survey of fungi in activated sludge plants with membrane bioreactors (MBRs). Thirty-six samples of both aerobic and anoxic activated sludge were taken from two plants with MBRs treating domestic wastewater. Over a period of 8 months, two samples from each plant were take...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2010.01959.x

    authors: Awad MF,Kraume M

    更新日期:2011-09-01 00:00:00

  • Comparison of two long-term gestagen regimens in the management of recurrent vulvovaginal candidiasis: A pilot study.

    abstract::Vulvovaginal candidiasis (VVC) is a hormonal-dependent infection but in contrast to sporadic VVC, therapy of recurrent vulvovaginal candidiasis (RVVC) is still unsolved. Long-term administration of medroxyprogesterone acetate was evaluated for the management of RVVC. Overall, 20 patients were treated with Depo-Provera...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12593

    authors: Špaček J,Kestřánek J,Jílek P,Leško D,Plucnarová S,Buchta V

    更新日期:2017-04-01 00:00:00

  • Malassezia species isolated from lesional and non-lesional skin in patients with pityriasis versicolor.

    abstract::Pityriasis versicolor (PV) is a superficial fungal infection where Malassezia species play a definite causative role, but the clinical significance of each of these species is not fully understood. The aim of our study was to analyse the prevalence of Malassezia species in PV lesions and to examine if the range of spe...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2006.01310.x

    authors: Prohic A,Ozegovic L

    更新日期:2007-01-01 00:00:00

  • Anti-Malassezia furfur antibodies in the population.

    abstract::The authors carried out research into anti-Malassezia precipitating antibodies in the population, specifying the distribution by age and sex. A total of 868 serum specimens from subjects of both sexes, aged between 0 and 80 years, were studied. An immunoelectroprecipitation reaction was used using a M. furfur culture ...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1998.tb00337.x

    authors: Faggi E,Pini G,Campisi E,Gargani G

    更新日期:1998-09-01 00:00:00

  • Biotyping of Candida strains with regard to the epidemiology of candidosis. A practical approach.

    abstract::In 62 strains of Candida albicans cultivated from specimens of patients with recurrent vaginal candidosis or with renal transplants, the biotypes were determined according to several characters: colony morphology, production of chlamydospores, auxanogram of C- and N-substances, zymogram, growth kinetics, adherence cap...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.1990.33.11-12.527

    authors: Tomsíková A,Vraná D,Kotál L

    更新日期:1990-11-01 00:00:00

  • Adherence of Malassezia furfur to human stratum corneum cells in vitro: a study of healthy individuals and patients with seborrhoeic dermatitis.

    abstract::The role of Malassezia furfur adherence to human stratum corneum cells in vitro was studied. The adherence assay was performed with one strain of M. furfur and stratum corneum cells from 30 healthy individuals and from 28 patients with seborrhoeic dermatitis. Stratum corneum cells from patients with seborrhoeic dermat...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1994.tb00303.x

    authors: Bergbrant IM,Faergemann J

    更新日期:1994-06-01 00:00:00

  • Clinical and pharmacokinetic investigations of oral terbinafine in patients with tinea unguium.

    abstract::A clinical trial of once-daily administration of a 125-mg tablet of terbinafine, an oral antimycotic agent, was performed on patients with tinea unguium to evaluate its efficacy, safety, possible side-effects and its incorporation into nails and hair. Thirty-four patients were recruited into the study. For the statist...

    journal_title:Mycoses

    pub_type: 临床试验,杂志文章

    doi:10.1111/j.1439-0507.1995.tb00037.x

    authors: Matsumoto T,Tanuma H,Kaneko S,Takasu H,Nishiyama S

    更新日期:1995-03-01 00:00:00

  • Ocular tolerance of sertaconazole gel.

    abstract::The in vitro and in vivo tolerance of sertaconazole gel, a new topical azole antifungal, was studied. Ketoconazole gel (Panfungol) was used as a reference substance. The methods applied for tolerance assessment were the bovine corneal opacity and permeability test for the in vitro assay and a modified Draize test for ...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1996.tb00085.x

    authors: Romero A,Grau MT,Villamayor F,Zapatero J,Mayordomo L,Tortajada A,Sacristán A,Ortiz JA

    更新日期:1996-01-01 00:00:00

  • How can we optimise antifungal use in a solid organ transplant centre? Local epidemiology and antifungal stewardship implementation: A single-centre study.

    abstract:PURPOSE:We aimed to implement and to assess the impact of the antifungal stewardship programme (AFSp) on prescription appropriateness of antifungals, management and outcomes of candidaemia patients, and antifungal consumption and costs at our solid organ transplant (SOT) institute. METHODS:Local epidemiology of invasi...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.13098

    authors: Mularoni A,Adamoli L,Polidori P,Ragonese B,Gioè SM,Pietrosi A,Tuzzolino F,Guadagnino G,Monaco F,Grossi PA,Conaldi PG,Luca A,Mikulska M

    更新日期:2020-07-01 00:00:00

  • Clinical presentation of invasive aspergillosis.

    abstract::Invasive aspergillosis has increasingly been recognised to cause significant morbidity and mortality in immunocompromised patients. Fever unresponsive to broad-spectrum antibiotics is the earliest and most common sign of an invasive fungal infection. As invasive Aspergillus infections are usually acquired by inhalatio...

    journal_title:Mycoses

    pub_type: 杂志文章,评审

    doi:10.1111/j.1439-0507.1997.tb00559.x

    authors: Schwartz S,Thiel E

    更新日期:1997-01-01 00:00:00

  • Inhibitory effect of terbinafine on reactive oxygen species (ROS) generation by Candida albicans.

    abstract::Candida albicans, the most important opportunistic fungal pathogen, is able to generate remarkable amounts of reactive oxygen species (ROS). Since ROS are highly cytotoxic, this mechanism may contribute to the pathogenicity of this yeast, including its invasiveness and the inflammatory response of the host. Terbinafin...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1046/j.1439-0507.2002.00756.x

    authors: Sander CS,Hipler UC,Wollina U,Elsner P

    更新日期:2002-06-01 00:00:00

  • Is the extraction by Whatman FTA filter matrix technology and sequencing of large ribosomal subunit D1-D2 region sufficient for identification of clinical fungi?

    abstract::Although conventional identification of pathogenic fungi is based on the combination of tests evaluating their morphological and biochemical characteristics, they can fail to identify the less common species or the differentiation of closely related species. In addition these tests are time consuming, labour-intensive...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12365

    authors: Kiraz N,Oz Y,Aslan H,Erturan Z,Ener B,Akdagli SA,Muslumanoglu H,Cetinkaya Z

    更新日期:2015-10-01 00:00:00

  • Detection of Candida dubliniensis in oropharyngeal samples from human immunodeficiency virus infected and non-infected patients and in a yeast culture collection.

    abstract::The incidence of Candida dubliniensis in oropharyngeal swabs of 132 human immunodeficiency virus (HIV)-positive and 89 HIV-negative patients was determined. The samples were plated onto CHROMagar Candida medium and 82 strains, presumptively identified as C. albicans or C. dubliniensis, were further investigated (tempe...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2005.01129.x

    authors: Faggi E,Pini G,Campisi E,Martinelli C,Difonzo E

    更新日期:2005-05-01 00:00:00

  • Risk factors for candidaemia: A prospective multi-centre case-control study.

    abstract:OBJECTIVES:Candidaemia carries a mortality of up to 40% and may be related to increasing complexity of medical care. Here, we determined risk factors for the development of candidaemia. METHODS:We conducted a prospective, multi-centre, case-control study over 12 months. Cases were aged ≥18 years with at least one bloo...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.13211

    authors: Keighley CL,Pope A,Marriott DJE,Chapman B,Bak N,Daveson K,Hajkowicz K,Halliday C,Kennedy K,Kidd S,Sorrell TC,Underwood N,van Hal S,Slavin MA,Chen SC

    更新日期:2020-11-13 00:00:00

  • African histoplasmosis in a Belgian AIDS patient.

    abstract::We describe an African histoplasmosis case in a 65-year-old Belgian living in Zaire for 46 years. Microscopic bone marrow examination showed Histoplasma. It was confirmed by cultures and hamster inoculation. Autopsy revealed a fungal dissemination by Histoplasma duboisii. ...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1991.tb00620.x

    authors: Arendt V,Coremans-Pelseneer J,Gottlob R,Bril T,Bujan-Boza W,Fondu P

    更新日期:1991-01-01 00:00:00

  • Genotype distribution of Candida albicans isolates by 25S intron analysis with regard to invasiveness.

    abstract::The aim of this study was to genotype Candida albicans strains isolated from patients with invasive and non-invasive deep-seated infections. For this purpose, 301 C. albicans isolates (81 invasive and 220 non-invasive) were genotyped by using specific PCR primers designed to span the transposable group I intron of the...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2004.01022.x

    authors: Karahan ZC,Güriz H,Ağirbaşli H,Balaban N,Göçmen JS,Aysev D,Akar N

    更新日期:2004-12-01 00:00:00

  • Comparison of morphotypic and genotypic methods for strain delineation in Candida.

    abstract::We compared two phenotypic methods, colony morphotyping on Sabouraudtripheniltetrazolium agar (STTZ) and serotyping, with two genotypic methods, karyotyping and Random Amplified Polymorphic DNA bands obtained by PCR amplification (RAPD-PCR), for strain delineation in 33 Candida clinical isolates and two C. albicans st...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1997.tb00182.x

    authors: Del Castillo L,Bikandi J,Nieto A,Quindós G,Sentandreu R,Pontón J

    更新日期:1997-12-01 00:00:00

  • Superficial mycoses due to Trichophyton violaceum in Athens, Greece: a 15-year retrospective study.

    abstract::This is a retrospective study on the superficial mycoses due to Trichophyton violaceum in the greater Athens area for the last 15 years - 1989-2003. During this period 104 patients were found to have an infection due to T. violaceum- an incidence rate of 0.75% of all dermatophytosis. Of the patients 59 were Greeks, 15...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2005.01159.x

    authors: Frangoulis E,Papadogeorgakis H,Athanasopoulou B,Katsambas A

    更新日期:2005-11-01 00:00:00

  • Aspergillosis of bones and joints - a review from 2002 until today.

    abstract::Poor clinical outcome and complicated neurological complications illustrate the severity of bone and joint infections with Aspergillus species. Host predisposing conditions are immunosuppression, intravenous drug use, a variety of chronic underlying diseases and prior surgical interventions. Nosocomial infections may ...

    journal_title:Mycoses

    pub_type: 杂志文章,评审

    doi:10.1111/myc.12165

    authors: Koehler P,Tacke D,Cornely OA

    更新日期:2014-06-01 00:00:00

  • Genotypic relatedness of yeast isolates from women infected with human immunodeficiency virus and their children.

    abstract::The objective of this study was to compare polymerase chain reaction (PCR) fingerprinting with other molecular typing methods as an epidemiologic tool to investigate the transmission of Candida strains between HIV-positive mothers and their children. Forty-nine yeast strains (including Candida albicans, Candida glabra...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1046/j.1439-0507.1999.00479.x

    authors: Ruhnke M,Grosch-Wörner I,Lischewski A,Neubauer A,Steinmüller A,Trautmann M,Morschhäuser J

    更新日期:1999-01-01 00:00:00