Production of extracellular collagenolytic proteinases by Histoplasma capsulatum var. duboisii and Histoplasma capsulatum var. capsulatum in the yeast phase.

Abstract:

:Yeast cultures of Histoplasma capsulatum var. duboisii and H. capsulatum var. capsulatum in collagen containing defined, semi-defined and complex media produced extracellular collagenolytic proteinases, assayed using 4-phenylazo-benzyloxycarbonyl-L-propyl-L-leucyl- glycyl-L-propyl-D-arginine, a specific collagenase substrate. Significant levels of hydroxyproline were measured in the cultures and clear zones of hydrolysis were produced in collagen buffer agar by the crude enzyme preparations. Hydrolysis of casein and bovine serum albumin at pH 8 suggests the presence, in the crude enzymes, of multiple proteinases rather than a collagenase with broad substrate specificity since collagenolytic activity was not detected at pH 5 and above. Collagenolytic activities in the crude enzymes of both fungi were optimal at pH 4, 40 degrees C and were inhibited by EDTA, phosphoramidion and aprotinin indicating a metallo-serine nature. The molecular weights, estimated by column chromatography, were both 17 kD. The enzymes probably constitute a shared antigen. A probable role in the pathogenesis of histoplasmosis is discussed.

journal_name

Mycoses

journal_title

Mycoses

authors

Okeke CN,Müller J

doi

10.1111/j.1439-0507.1991.tb00860.x

subject

Has Abstract

pub_date

1991-11-01 00:00:00

pages

453-60

issue

11-12

eissn

0933-7407

issn

1439-0507

journal_volume

34

pub_type

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