Study of the ITS region in an atypical isolate and comparison with six species of Microsporum.

Abstract:

:Microsporum species are a frequent cause of cutaneous mycoses in humans. Atypical strains of Microsporum can sometimes be difficult to identify with conventional methods. Recently, we have obtained a Microsporum isolate with atypical morphology and special nutritional requirements (Microsporum CHUS-126-02). As several molecular techniques have been developed for the identification of fungi, we analysed six Microsporum species (M. canis, M. gypseum, M. gallinae, M. nanum, M. ferrugineum and M. persicolor) in order to compare them with our isolate, by using polymerase chain reaction-restriction enzyme analysis (PCR-REA). We studied the nucleotide sequence of the internal transcribed spacer regions from the nuclear DNA encoding for the ribosomal domain. Digestion with MvaI and EcoRI endonucleases obtained specific patterns for M. gypseum, M. gallinae, M. nanum and M. persicolor. Microsporum canis, M. ferrugineum and Microsporum CHUS-126-02 yielded the same patterns. Based on these results and phenotypic criteria, we classified our atypical isolate as M. canis.

journal_name

Mycoses

journal_title

Mycoses

authors

Leon-Mateos A,Paredes-Suárez C,Pereiro M Jr,Toribio J

doi

10.1111/j.1439-0507.2006.01281.x

subject

Has Abstract

pub_date

2006-11-01 00:00:00

pages

452-6

issue

6

eissn

0933-7407

issn

1439-0507

pii

MYC1281

journal_volume

49

pub_type

杂志文章

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