Extracellular protease expression in Microsporum gypseum complex, its regulation and keratinolytic potential.

Abstract:

:Two soil isolates of Microsporum gypseum were studied for the production of extracellular proteases. Both the strains secreted protease on glucose-gelatin medium. The enzyme activity peaked on day 15 at 28 °C. Asparagine repressed protease yield. Sugars caused catabolite repression of protease formation. Protease activities of both the isolates were significantly affected by incubation period, culture media and carbohydrates used. Both the strains grew on the skin bait and caused a gravimetrically measurable loss of the substrate. Despite less pronounced differences in the keratinase levels, great variations occurred in the amount of keratin degraded by two isolates. Keratinase production as well as loss in substrate mass was better in glucose-lacking flasks than those containing the sugar. Although the rate of keratin degradation was independent of enzyme production, statistically positive correlations were recorded between loss in substrate mass: yielded dry mycelial weight and substrate degradation: keratinase levels.

journal_name

Mycoses

journal_title

Mycoses

authors

Singh CJ

doi

10.1111/j.1439-0507.2010.01871.x

subject

Has Abstract

pub_date

2011-07-01 00:00:00

pages

e183-8

issue

4

eissn

0933-7407

issn

1439-0507

journal_volume

54

pub_type

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