Abstract:
:Invasive aspergillosis is a major cause of morbidity and mortality in immunocompromised and critically ill patients. Standard culture based methods for the diagnosis of Aspergillus infections have limited sensitivity and specificity and are time consuming. The recent availability of novel molecular based diagnostic techniques offers the potential of rapid, highly sensitive and specific pathogen detection. In this study, we aimed to develop a diagnostic assay to detect simultaneously common pathogenic Aspergillus species including Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger and Aspergillus terreus in whole blood specimen. A real-time PCR/probe set assay was designed to amplify the 18sRNA genomic region of Aspergillus. In addition to a previously probe set validated for the detection of A. fumigatus ('Asp.fum'), two other probe sets ('Asp.ter'; 'Asp.nig') were synthesised with 100% sequence homology to A. terreus or A. niger. Specificity testing demonstrated amplification of Aspergillus DNA, but not DNA from a panel of other common pathogenic fungi and bacteria and/or human DNA. Sensitivity testing in serial dilution assays revealed a lower detection limit of 1-5 CFU ml(-1) whole blood. However, no single probe set was most sensitive for all Aspergillus species tested and a combination of all three probe sets was necessary to achieve maximal overall assay sensitivity. Furthermore, inclusion of a subsequent probe melting temperature (Tm) analysis demonstrated species-specific Tm-patterns, useful to differentiate simultaneously between the different Aspergillus species detected. We describe a highly specific and sensitive real-time PCR approach to detect and differentiate the most common pathogenic Aspergillus species in a rapid 'same day' assay.
journal_name
Mycosesjournal_title
Mycosesauthors
Faber J,Moritz N,Henninger N,Zepp F,Knuf Mdoi
10.1111/j.1439-0507.2008.01565.xsubject
Has Abstractpub_date
2009-05-01 00:00:00pages
228-33issue
3eissn
0933-7407issn
1439-0507pii
MYC1565journal_volume
52pub_type
杂志文章相关文献
MYCOSES文献大全abstract::The aims of the study were to define the duration of candidaemia in newborn infants and to determine the incidence of persistent hospital-acquired candidaemia and its associated morbidity and mortality compared with non-persistent candidaemia. This retrospective study, included 56 neonates admitted to the neonatal int...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2006.01231.x
更新日期:2006-05-01 00:00:00
abstract::Candida albicans was maintained in various culture media and incubated with different concentrations of the antifungal agent rilopirox. After fixation, dehydration and embedding in Spurr's medium, the cells were analysed at the ultrastructural level to investigate morphological aspects of the antifungal mode of action...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.1993.tb00727.x
更新日期:1993-11-01 00:00:00
abstract::Pomegranate is a wonderful fruit from the paradise which contains a wide variety of precious phytochemical compounds applicable in the fields of therapeutics and health care. Candida albicans is the most common etiological agent for many clinical mycoses which could lead to human and animal death. Determination of the...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2008.01681.x
更新日期:2010-03-01 00:00:00
abstract:BACKGROUND:Histoplasmosis is a frequent fungal infection in HIV/AIDS patients, with high morbimortality rates when diagnosis and treatment are delayed. Antibody detection, which is faster than the gold standard culture test, hastens the laboratory investigation. OBJECTIVES:To evaluate the role of WB for antibody detec...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.12877
更新日期:2019-03-01 00:00:00
abstract:BACKGROUND:Invasive pulmonary aspergillosis (IPA) is a complication of respiratory bacterial and viral infections such as coronavirus disease 2019 (COVID-19). PATIENTS/METHODS:In University Hospital La Paz (Madrid, Spain), we reviewed the clinical and demographic characteristics of 10 patients with positive severe acu...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.13155
更新日期:2020-08-04 00:00:00
abstract::Oral, pharyngeal and esophageal infections caused by Candida species occur very frequently when it comes to radiation therapy for head and neck cancer. In clinical terms oral candidosis is most often characterized by pseudomembranes or erythema. The erythematous form of oral candidosis has in particular to be distingu...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0507.1989.tb02308.x
更新日期:1989-01-01 00:00:00
abstract::The objective of this study was to compare polymerase chain reaction (PCR) fingerprinting with other molecular typing methods as an epidemiologic tool to investigate the transmission of Candida strains between HIV-positive mothers and their children. Forty-nine yeast strains (including Candida albicans, Candida glabra...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1046/j.1439-0507.1999.00479.x
更新日期:1999-01-01 00:00:00
abstract::An in vitro microassay for the measurement of Candida albicans hyphal-form growth inhibition by myelomonocytic cells is described. The assay is rapid, easy-to-perform and objective. A Candida strain capable of in vitro dimorphic transition from yeast to hyphal form has been employed. The assay is based on the incorpor...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.1991.tb00631.x
更新日期:1991-03-01 00:00:00
abstract::Outpatient parenteral antimicrobial therapy (OPAT) is an established treatment option for patients with a variety of infections who require a period of intravenous therapy, are clinically stable, and do not require continuous monitoring. Many patients with fungal infections require prolonged therapy due to resistance ...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/myc.12874
更新日期:2019-03-01 00:00:00
abstract::To date, there has been only one published report on the infectious sclerokeratitis caused by Metarhizium anisopliae, which is an entomopathogenic fungus. Regarding corneal infection, three reports have been published to date. Although the prognoses of the corneal infections are favourable, prognosis when scleral infe...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/myc.12279
更新日期:2015-02-01 00:00:00
abstract::Black Aspergilli are widely distributed in the environment and are frequently reported as causative agents of different types of mycoses. Many taxonomical revisions have been made, and presently 19 different species are accepted. In this study we (re-) identified 123 strains of the Aspergillus niger group of the BCCM/...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2011.02049.x
更新日期:2012-03-01 00:00:00
abstract::This retrospective study reviews the occurrence and treatment of various forms of tinea capitis at the out-patient clinic for Dermatology and Pediatric Dermatology of the University Hospital Sophia/Dijkzigt over the period 1977-1988. Tinea capitis was diagnosed in 64 children. Trichophyton violaceum was the pathogen m...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.1990.33.1.46
更新日期:1990-01-01 00:00:00
abstract::A 38-year-old woman infected with human immunodeficiency virus (HIV) presented with persistent oral candidosis in which non-albicans Candida strains were the predominant yeasts in most of the examinations performed over a period of 6 years. Oral treatment with fluconazole had no effect on clinical signs of oral candid...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.1998.tb00347.x
更新日期:1998-09-01 00:00:00
abstract::Eighteen adult white male patients with cutaneous sporotrichosis were treated with itraconazole following different daily dose schemes. Cure was obtained in all cases after periods of 15-75 days (median 44 days) with total doses between 3.1 and 14.8 g (median 8.4 g). No serious side effects were observed and no relaps...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.1992.tb00836.x
更新日期:1992-05-01 00:00:00
abstract::Due to the Fourier-Transform Infrared Spectroscopy (FT-IR) of strain specific traits demonstrated to be a suitable and efficient method for diagnostic and epidemiological determinations for the yeasts Candida albicans, Exophiala dermatitidis and the chlorophylless algae of the genus Prototheca. FT-IR leads in a rapid ...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.1998.tb00587.x
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND:The incidence of fungal keratitis has increased in recent years. While the epidemiology and clinical roles of various Candida and Fusarium species have been relatively well-identified in infections of the eye, data regarding keratitis caused by Aspergillus species are scant. Accurate and rapid diagnosis is i...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.12855
更新日期:2019-02-01 00:00:00
abstract::Despite the existence of endemic mycoses in Latin America and the Caribbean, in addition to a large population of patients at risk for invasive mycoses, the capability of medical centres to perform a proper diagnosis in mycology has not been studied in the region. Moreover, availability of antifungal drugs in the regi...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.12890
更新日期:2019-04-01 00:00:00
abstract::The incidence and prevalence of fungal infections in Tanzania remains unknown. We assessed the annual burden in the general population and among populations at risk. Data were extracted from 2012 reports of the Tanzanian AIDS program, WHO, reports, Tanzanian census, and from a comprehensive PubMed search. We used mode...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.12390
更新日期:2015-10-01 00:00:00
abstract::Forty soil samples from different desert localities in Kuwait were surveyed for keratinophilic and geophilic dermatophytic fungi. Sixteen species classified in 9 genera were recovered on wool baits. Chrysosporium species were the most common fungi: Chr. tropicum (60%), Chr. pannicola (32.5%), Chr. queenslandicum (27.5...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.1989.tb02247.x
更新日期:1989-06-01 00:00:00
abstract::Prior clinical trials have demonstrated efficacy and effectiveness of posaconazole in the prophylaxis of invasive fungal diseases in high-risk patients. Controversy exists about the cost-effectiveness of this approach. We performed an analysis comparing the direct costs of posaconazole prophylaxis against polyene mout...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.12105
更新日期:2014-02-01 00:00:00
abstract::Invasive pulmonary aspergillosis is a serious threat to immunocompromised and critical care patients. Recent detailed guidelines and treatment algorithms lead microbiologists and clinicians in diagnosis and treatment of invasive aspergillosis. Currently, there is no tool available that allows to measure guideline adhe...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.12820
更新日期:2018-11-01 00:00:00
abstract::Invasive candidiasis is a common nosocomial infection among critically ill patients, constitutes an important cause of sepsis, and is associated with significant morbidity and mortality. The Infectious Diseases Society of America (IDSA) has created evidence-based guidelines for the management of invasive candidiasis. ...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0507.2008.01571.x
更新日期:2008-09-01 00:00:00
abstract:BACKGROUND:Pulmonary cryptococcosis (PC) is not considered an rare, opportunistic infection anymore. The immunocompetent population accounts for an increasing proportion of the morbidity. OBJECTIVE:This study investigated the clinical characteristics of PC patients spanning 20 years, in a referral centre of China. PA...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.12966
更新日期:2019-10-01 00:00:00
abstract::The standard methods for yeast susceptibility testing require 24-48 h of incubation. As there has been an increase in incidence of non-albicans Candida species, the clinician is very often wary of initiating therapy with fluconazole until a final susceptibility report is generated, especially when treating very sick p...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2007.01466.x
更新日期:2008-05-01 00:00:00
abstract::The study was performed to analyse the spectrum of dermatomycoses in southwest Poland during the period 2003-2007. A total of 10,486 patients were investigated for fungal skin infections by means of native specimen and cultivating procedures. Skin scrapings, plucked hairs and nail clippings were examined and identifie...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2009.01774.x
更新日期:2011-03-01 00:00:00
abstract::Despite appropriate antimicrobial and antifungal therapy (amphotericin B), a disseminated infection with Fusarium oxysporum in a 75-year-old immunocompromised patient (acute myeloid leukaemia, minimal leucocyte count of 0.5 giga l-1) led rapidly to death. A similarly fatal course of an F. oxysporum infection has been ...
journal_title:Mycoses
pub_type: 信件
doi:10.1046/j.1439-0507.2000.00590.x
更新日期:2000-10-01 00:00:00
abstract::The objective of the present study was to report on three distinct forms of presentation of Cryptococcus neoformans infection in three cirrhotic patients. One patient had disseminated cryptococcosis with detection of the fungus in ascitic fluid, cerebrospinal fluid and blood; the second patient had pleural involvement...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2004.01064.x
更新日期:2005-01-01 00:00:00
abstract::New antifungal agents are needed to match the currently increasing rate of systemic fungal infections and the development of resistant fungal strains. This appears possible by the introduction of second generation azole antifungals which potently inhibit ergosterol synthesis, but also by a partial synthetic echinocand...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:
更新日期:2003-01-01 00:00:00
abstract::Pityriasis versicolor (PV) is a superficial fungal infection where Malassezia species play a definite causative role, but the clinical significance of each of these species is not fully understood. The aim of our study was to analyse the prevalence of Malassezia species in PV lesions and to examine if the range of spe...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2006.01310.x
更新日期:2007-01-01 00:00:00
abstract::Regulation of morphogenesis through the production of chemical signalling molecules such as isoamyl alcohol, 2-phenylethyl alcohol, 1-dodecanol, E-nerolidol and farnesol is reported in Candida albicans. The present study focuses on the effect of ethyl alcohol on C. albicans dimorphism and biofilm development. Ethyl al...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2010.02002.x
更新日期:2011-11-01 00:00:00