Rapid detection of common pathogenic Aspergillus species by a novel real-time PCR approach.

Abstract:

:Invasive aspergillosis is a major cause of morbidity and mortality in immunocompromised and critically ill patients. Standard culture based methods for the diagnosis of Aspergillus infections have limited sensitivity and specificity and are time consuming. The recent availability of novel molecular based diagnostic techniques offers the potential of rapid, highly sensitive and specific pathogen detection. In this study, we aimed to develop a diagnostic assay to detect simultaneously common pathogenic Aspergillus species including Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger and Aspergillus terreus in whole blood specimen. A real-time PCR/probe set assay was designed to amplify the 18sRNA genomic region of Aspergillus. In addition to a previously probe set validated for the detection of A. fumigatus ('Asp.fum'), two other probe sets ('Asp.ter'; 'Asp.nig') were synthesised with 100% sequence homology to A. terreus or A. niger. Specificity testing demonstrated amplification of Aspergillus DNA, but not DNA from a panel of other common pathogenic fungi and bacteria and/or human DNA. Sensitivity testing in serial dilution assays revealed a lower detection limit of 1-5 CFU ml(-1) whole blood. However, no single probe set was most sensitive for all Aspergillus species tested and a combination of all three probe sets was necessary to achieve maximal overall assay sensitivity. Furthermore, inclusion of a subsequent probe melting temperature (Tm) analysis demonstrated species-specific Tm-patterns, useful to differentiate simultaneously between the different Aspergillus species detected. We describe a highly specific and sensitive real-time PCR approach to detect and differentiate the most common pathogenic Aspergillus species in a rapid 'same day' assay.

journal_name

Mycoses

journal_title

Mycoses

authors

Faber J,Moritz N,Henninger N,Zepp F,Knuf M

doi

10.1111/j.1439-0507.2008.01565.x

subject

Has Abstract

pub_date

2009-05-01 00:00:00

pages

228-33

issue

3

eissn

0933-7407

issn

1439-0507

pii

MYC1565

journal_volume

52

pub_type

杂志文章

相关文献

MYCOSES文献大全
  • Duration and outcome of persistent candidaemia in newborn infants.

    abstract::The aims of the study were to define the duration of candidaemia in newborn infants and to determine the incidence of persistent hospital-acquired candidaemia and its associated morbidity and mortality compared with non-persistent candidaemia. This retrospective study, included 56 neonates admitted to the neonatal int...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2006.01231.x

    authors: Levy I,Shalit I,Askenazi S,Klinger G,Sirota L,Linder N

    更新日期:2006-05-01 00:00:00

  • Ultrastructural investigations of Candida albicans after in vitro treatment with the new fungicidal agent rilopirox.

    abstract::Candida albicans was maintained in various culture media and incubated with different concentrations of the antifungal agent rilopirox. After fixation, dehydration and embedding in Spurr's medium, the cells were analysed at the ultrastructural level to investigate morphological aspects of the antifungal mode of action...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1993.tb00727.x

    authors: Reitze HK,Dannhorn DR,Hänel H,Seitz KA

    更新日期:1993-11-01 00:00:00

  • Anticandidal activity of pomegranate peel extract aerosol as an applicable sanitizing method.

    abstract::Pomegranate is a wonderful fruit from the paradise which contains a wide variety of precious phytochemical compounds applicable in the fields of therapeutics and health care. Candida albicans is the most common etiological agent for many clinical mycoses which could lead to human and animal death. Determination of the...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2008.01681.x

    authors: Tayel AA,El-Tras WF

    更新日期:2010-03-01 00:00:00

  • Role of western blot assay for the diagnosis of histoplasmosis in AIDS patients from a National Institute of Infectious Diseases in Rio de Janeiro, Brazil.

    abstract:BACKGROUND:Histoplasmosis is a frequent fungal infection in HIV/AIDS patients, with high morbimortality rates when diagnosis and treatment are delayed. Antibody detection, which is faster than the gold standard culture test, hastens the laboratory investigation. OBJECTIVES:To evaluate the role of WB for antibody detec...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12877

    authors: Almeida MA,Damasceno LS,Pizzini CV,Muniz MM,Almeida-Paes R,Zancopé-Oliveira RM

    更新日期:2019-03-01 00:00:00

  • Isolation of Aspergillus spp. in respiratory samples of patients with COVID-19 in a Spanish Tertiary Care Hospital.

    abstract:BACKGROUND:Invasive pulmonary aspergillosis (IPA) is a complication of respiratory bacterial and viral infections such as coronavirus disease 2019 (COVID-19). PATIENTS/METHODS:In University Hospital La Paz (Madrid, Spain), we reviewed the clinical and demographic characteristics of 10 patients with positive severe acu...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.13155

    authors: Falces-Romero I,Ruiz-Bastián M,Díaz-Pollán B,Maseda E,García-Rodríguez J,SARS-CoV-2 Working Group.

    更新日期:2020-08-04 00:00:00

  • The role of Candida infections as an adverse effect upon head and neck cancer patients undergoing therapeutic radiation and the effect of antimycotic treatment.

    abstract::Oral, pharyngeal and esophageal infections caused by Candida species occur very frequently when it comes to radiation therapy for head and neck cancer. In clinical terms oral candidosis is most often characterized by pseudomembranes or erythema. The erythematous form of oral candidosis has in particular to be distingu...

    journal_title:Mycoses

    pub_type: 杂志文章,评审

    doi:10.1111/j.1439-0507.1989.tb02308.x

    authors: Poland JM

    更新日期:1989-01-01 00:00:00

  • Genotypic relatedness of yeast isolates from women infected with human immunodeficiency virus and their children.

    abstract::The objective of this study was to compare polymerase chain reaction (PCR) fingerprinting with other molecular typing methods as an epidemiologic tool to investigate the transmission of Candida strains between HIV-positive mothers and their children. Forty-nine yeast strains (including Candida albicans, Candida glabra...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1046/j.1439-0507.1999.00479.x

    authors: Ruhnke M,Grosch-Wörner I,Lischewski A,Neubauer A,Steinmüller A,Trautmann M,Morschhäuser J

    更新日期:1999-01-01 00:00:00

  • A rapid Candida albicans hyphal-form growth inhibition assay: determination of myelomonocytic-mediated antifungal activity.

    abstract::An in vitro microassay for the measurement of Candida albicans hyphal-form growth inhibition by myelomonocytic cells is described. The assay is rapid, easy-to-perform and objective. A Candida strain capable of in vitro dimorphic transition from yeast to hyphal form has been employed. The assay is based on the incorpor...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1991.tb00631.x

    authors: Scaringi L,Blasi E,Cornacchione P,Bietta C,Bistoni F

    更新日期:1991-03-01 00:00:00

  • Can intravenous antifungal therapy be safely used in the outpatient parenteral antimicrobial therapy (OPAT) setting?

    abstract::Outpatient parenteral antimicrobial therapy (OPAT) is an established treatment option for patients with a variety of infections who require a period of intravenous therapy, are clinically stable, and do not require continuous monitoring. Many patients with fungal infections require prolonged therapy due to resistance ...

    journal_title:Mycoses

    pub_type: 杂志文章,评审

    doi:10.1111/myc.12874

    authors: Rae N,Kenny C,Muldoon EG

    更新日期:2019-03-01 00:00:00

  • Severe fungal sclerokeratitis caused by Metarhizium anisopliae: a case report and literature review.

    abstract::To date, there has been only one published report on the infectious sclerokeratitis caused by Metarhizium anisopliae, which is an entomopathogenic fungus. Regarding corneal infection, three reports have been published to date. Although the prognoses of the corneal infections are favourable, prognosis when scleral infe...

    journal_title:Mycoses

    pub_type: 杂志文章,评审

    doi:10.1111/myc.12279

    authors: Eguchi H,Toibana T,Hotta F,Miyamoto T,Mitamura Y,Yaguchi T

    更新日期:2015-02-01 00:00:00

  • Genetic re-identification and antifungal susceptibility testing of Aspergillus section Nigri strains of the BCCM/IHEM collection.

    abstract::Black Aspergilli are widely distributed in the environment and are frequently reported as causative agents of different types of mycoses. Many taxonomical revisions have been made, and presently 19 different species are accepted. In this study we (re-) identified 123 strains of the Aspergillus niger group of the BCCM/...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2011.02049.x

    authors: Hendrickx M,Beguin H,Detandt M

    更新日期:2012-03-01 00:00:00

  • Tinea capitis in The Netherlands (Rotterdam area).

    abstract::This retrospective study reviews the occurrence and treatment of various forms of tinea capitis at the out-patient clinic for Dermatology and Pediatric Dermatology of the University Hospital Sophia/Dijkzigt over the period 1977-1988. Tinea capitis was diagnosed in 64 children. Trichophyton violaceum was the pathogen m...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.1990.33.1.46

    authors: van der Willigen AH,Oranje AP,de Weerdt-van Ameijden S,Wagenvoort JH

    更新日期:1990-01-01 00:00:00

  • Persistent oral candidosis by non-albicans Candida strains including Candida glabrata in a human immunodeficiency virus-infected patient observed over a period of 6 years.

    abstract::A 38-year-old woman infected with human immunodeficiency virus (HIV) presented with persistent oral candidosis in which non-albicans Candida strains were the predominant yeasts in most of the examinations performed over a period of 6 years. Oral treatment with fluconazole had no effect on clinical signs of oral candid...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1998.tb00347.x

    authors: Hoegl L,Thoma-Greber E,Röcken M,Korting HC

    更新日期:1998-09-01 00:00:00

  • Treatment of human cutaneous sporotrichosis with itraconazole.

    abstract::Eighteen adult white male patients with cutaneous sporotrichosis were treated with itraconazole following different daily dose schemes. Cure was obtained in all cases after periods of 15-75 days (median 44 days) with total doses between 3.1 and 14.8 g (median 8.4 g). No serious side effects were observed and no relaps...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1992.tb00836.x

    authors: Conti Díaz IA,Civila E,Gezuele E,Lowinger M,Calegari L,Sanabria D,Fuentes L,Da Rosa D,Alzueta G

    更新日期:1992-05-01 00:00:00

  • [Differentiation and characterization of yeasts pathogenic for humans (Candida albicans, Exophiala dermatitidis) and algae pathogenic for animals (Prototheca spp.) using Fourier transform infrared spectroscopy (FTIR) in comparison with conventional method

    abstract::Due to the Fourier-Transform Infrared Spectroscopy (FT-IR) of strain specific traits demonstrated to be a suitable and efficient method for diagnostic and epidemiological determinations for the yeasts Candida albicans, Exophiala dermatitidis and the chlorophylless algae of the genus Prototheca. FT-IR leads in a rapid ...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1998.tb00587.x

    authors: Schmalreck AF,Tränkle P,Vanca E,Blaschke-Hellmessen R

    更新日期:1998-01-01 00:00:00

  • Epidemiology of Aspergillus species causing keratitis in Mexico.

    abstract:BACKGROUND:The incidence of fungal keratitis has increased in recent years. While the epidemiology and clinical roles of various Candida and Fusarium species have been relatively well-identified in infections of the eye, data regarding keratitis caused by Aspergillus species are scant. Accurate and rapid diagnosis is i...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12855

    authors: Al-Hatmi AMS,Castro MA,de Hoog GS,Badali H,Alvarado VF,Verweij PE,Meis JF,Zago VV

    更新日期:2019-02-01 00:00:00

  • Clinical mycology in Latin America and the Caribbean: A snapshot of diagnostic and therapeutic capabilities.

    abstract::Despite the existence of endemic mycoses in Latin America and the Caribbean, in addition to a large population of patients at risk for invasive mycoses, the capability of medical centres to perform a proper diagnosis in mycology has not been studied in the region. Moreover, availability of antifungal drugs in the regi...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12890

    authors: Falci DR,Pasqualotto AC

    更新日期:2019-04-01 00:00:00

  • Burden of serious fungal infections in Tanzania.

    abstract::The incidence and prevalence of fungal infections in Tanzania remains unknown. We assessed the annual burden in the general population and among populations at risk. Data were extracted from 2012 reports of the Tanzanian AIDS program, WHO, reports, Tanzanian census, and from a comprehensive PubMed search. We used mode...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12390

    authors: Faini D,Maokola W,Furrer H,Hatz C,Battegay M,Tanner M,Denning DW,Letang E

    更新日期:2015-10-01 00:00:00

  • Distribution of keratinophilic fungi in desert soil of Kuwait.

    abstract::Forty soil samples from different desert localities in Kuwait were surveyed for keratinophilic and geophilic dermatophytic fungi. Sixteen species classified in 9 genera were recovered on wool baits. Chrysosporium species were the most common fungi: Chr. tropicum (60%), Chr. pannicola (32.5%), Chr. queenslandicum (27.5...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.1989.tb02247.x

    authors: al-Musallam AA

    更新日期:1989-06-01 00:00:00

  • Treatment cost development of patients undergoing remission induction chemotherapy: a pharmacoeconomic analysis before and after introduction of posaconazole prophylaxis.

    abstract::Prior clinical trials have demonstrated efficacy and effectiveness of posaconazole in the prophylaxis of invasive fungal diseases in high-risk patients. Controversy exists about the cost-effectiveness of this approach. We performed an analysis comparing the direct costs of posaconazole prophylaxis against polyene mout...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12105

    authors: Heimann SM,Cornely OA,Vehreschild MJ,Glossmann J,Kochanek M,Kreuzer KA,Hallek M,Vehreschild JJ

    更新日期:2014-02-01 00:00:00

  • EQUAL Aspergillosis Score 2018: An ECMM score derived from current guidelines to measure QUALity of the clinical management of invasive pulmonary aspergillosis.

    abstract::Invasive pulmonary aspergillosis is a serious threat to immunocompromised and critical care patients. Recent detailed guidelines and treatment algorithms lead microbiologists and clinicians in diagnosis and treatment of invasive aspergillosis. Currently, there is no tool available that allows to measure guideline adhe...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12820

    authors: Cornely OA,Koehler P,Arenz D,C Mellinghoff S

    更新日期:2018-11-01 00:00:00

  • Invasive candidiasis in the ICU: evidence based and on the edge of evidence.

    abstract::Invasive candidiasis is a common nosocomial infection among critically ill patients, constitutes an important cause of sepsis, and is associated with significant morbidity and mortality. The Infectious Diseases Society of America (IDSA) has created evidence-based guidelines for the management of invasive candidiasis. ...

    journal_title:Mycoses

    pub_type: 杂志文章,评审

    doi:10.1111/j.1439-0507.2008.01571.x

    authors: Hollenbach E

    更新日期:2008-09-01 00:00:00

  • Pulmonary cryptococcosis characteristics in immunocompetent patients-A 20-year clinical retrospective analysis in China.

    abstract:BACKGROUND:Pulmonary cryptococcosis (PC) is not considered an rare, opportunistic infection anymore. The immunocompetent population accounts for an increasing proportion of the morbidity. OBJECTIVE:This study investigated the clinical characteristics of PC patients spanning 20 years, in a referral centre of China. PA...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/myc.12966

    authors: Hou X,Kou L,Han X,Zhu R,Song L,Liu T

    更新日期:2019-10-01 00:00:00

  • Direct fluconazole susceptibility testing of positive Candida blood cultures by flow cytometry.

    abstract::The standard methods for yeast susceptibility testing require 24-48 h of incubation. As there has been an increase in incidence of non-albicans Candida species, the clinician is very often wary of initiating therapy with fluconazole until a final susceptibility report is generated, especially when treating very sick p...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2007.01466.x

    authors: Rudensky B,Broide E,Berko N,Wiener-Well Y,Yinnon AM,Raveh D

    更新日期:2008-05-01 00:00:00

  • A 5-year survey of dermatomycoses in southwest Poland, years 2003-2007.

    abstract::The study was performed to analyse the spectrum of dermatomycoses in southwest Poland during the period 2003-2007. A total of 10,486 patients were investigated for fungal skin infections by means of native specimen and cultivating procedures. Skin scrapings, plucked hairs and nail clippings were examined and identifie...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2009.01774.x

    authors: Jankowska-Konsur A,Dyląg M,Hryncewicz-Gwóźdź A,Plomer-Niezgoda E,Szepietowski JC

    更新日期:2011-03-01 00:00:00

  • Case report. Pathohistological findings in a clinical case of disseminated infection with Fusarium oxysporum.

    abstract::Despite appropriate antimicrobial and antifungal therapy (amphotericin B), a disseminated infection with Fusarium oxysporum in a 75-year-old immunocompromised patient (acute myeloid leukaemia, minimal leucocyte count of 0.5 giga l-1) led rapidly to death. A similarly fatal course of an F. oxysporum infection has been ...

    journal_title:Mycoses

    pub_type: 信件

    doi:10.1046/j.1439-0507.2000.00590.x

    authors: Peltroche-Llacsahuanga H,Manegold E,Kroll G,Haase G

    更新日期:2000-10-01 00:00:00

  • Cryptococcosis in cirrhotic patients.

    abstract::The objective of the present study was to report on three distinct forms of presentation of Cryptococcus neoformans infection in three cirrhotic patients. One patient had disseminated cryptococcosis with detection of the fungus in ascitic fluid, cerebrospinal fluid and blood; the second patient had pleural involvement...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2004.01064.x

    authors: França AV,Carneiro M,dal Sasso K,Souza Cda S,Martinelli A

    更新日期:2005-01-01 00:00:00

  • [New systemic antifungal drugs: mechanisms of action, drug interactions and side effects].

    abstract::New antifungal agents are needed to match the currently increasing rate of systemic fungal infections and the development of resistant fungal strains. This appears possible by the introduction of second generation azole antifungals which potently inhibit ergosterol synthesis, but also by a partial synthetic echinocand...

    journal_title:Mycoses

    pub_type: 杂志文章,评审

    doi:

    authors: Schäfer-Korting M

    更新日期:2003-01-01 00:00:00

  • Malassezia species isolated from lesional and non-lesional skin in patients with pityriasis versicolor.

    abstract::Pityriasis versicolor (PV) is a superficial fungal infection where Malassezia species play a definite causative role, but the clinical significance of each of these species is not fully understood. The aim of our study was to analyse the prevalence of Malassezia species in PV lesions and to examine if the range of spe...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2006.01310.x

    authors: Prohic A,Ozegovic L

    更新日期:2007-01-01 00:00:00

  • A morphogenetic regulatory role for ethyl alcohol in Candida albicans.

    abstract::Regulation of morphogenesis through the production of chemical signalling molecules such as isoamyl alcohol, 2-phenylethyl alcohol, 1-dodecanol, E-nerolidol and farnesol is reported in Candida albicans. The present study focuses on the effect of ethyl alcohol on C. albicans dimorphism and biofilm development. Ethyl al...

    journal_title:Mycoses

    pub_type: 杂志文章

    doi:10.1111/j.1439-0507.2010.02002.x

    authors: Chauhan NM,Raut JS,Karuppayil SM

    更新日期:2011-11-01 00:00:00