Abstract:
:An in vitro microassay for the measurement of Candida albicans hyphal-form growth inhibition by myelomonocytic cells is described. The assay is rapid, easy-to-perform and objective. A Candida strain capable of in vitro dimorphic transition from yeast to hyphal form has been employed. The assay is based on the incorporation of 3H-glucose by the fungus, the effect being dependent upon the time of pulse, size of the inoculum and concentration of radiolabelled metabolite. In particular, C. albicans hyphal form, obtained by a 3 h incubation in vitro in the presence of 10% fetal calf serum, is co-incubated with the effector cells. A pulse with 3H-glucose in water is then performed and the radioactivity incorporated by the residual Candida is taken as an indication of hyphal growth. We found that polymorphonuclear cells, peritoneal macrophages and the cloned GG2EE macrophage cell line significantly inhibited hyphal growth, the effects being time and effector-to-target cell ratio dependent.
journal_name
Mycosesjournal_title
Mycosesauthors
Scaringi L,Blasi E,Cornacchione P,Bietta C,Bistoni Fdoi
10.1111/j.1439-0507.1991.tb00631.xsubject
Has Abstractpub_date
1991-03-01 00:00:00pages
119-23issue
3-4eissn
0933-7407issn
1439-0507journal_volume
34pub_type
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