[Molecular biological identification of Cuninghamella spec].

Abstract:

:Among the genus Cunninghamella, so far C. bertholletiae is known to be the only clinically relevant species. Correct identification of C. bertholletiae is not possible with classical methods. PCR and sequencing of the internal transcribed spacer (ITS) region was used to identify seven of nine clinical isolates as C. bertholletiae and two as C. echinulata. Also an isolate of the surrounding area of one patient infected with C. echinulata could be identified as C. echinulata. High homology in the ITS region was found within the isolates of C. bertholletiae. Within the species C. echinulata and C. elegans a differentiation on subspecies level was achieved by an analysis of restriction fragment length polymorphism of the ITS amplicons after incubation with TaqI and HinfI. Similar results were obtained by PCR fingerprinting of the complete DNA with the single microsatellite DNA primers (GTG)5 and (GAC)5. For the first time C. echinulata could be identified as agent of zygomycosis in humans.

journal_name

Mycoses

journal_title

Mycoses

authors

Lemmer K,Losert H,Rickerts V,Just-Nübling G,Sander A,Kerkmann ML,Tintelnot K

doi

10.1111/j.1439-0507.2002.tb04543.x

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

31-6

eissn

0933-7407

issn

1439-0507

journal_volume

45 Suppl 1

pub_type

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