Abstract:
:Among the genus Cunninghamella, so far C. bertholletiae is known to be the only clinically relevant species. Correct identification of C. bertholletiae is not possible with classical methods. PCR and sequencing of the internal transcribed spacer (ITS) region was used to identify seven of nine clinical isolates as C. bertholletiae and two as C. echinulata. Also an isolate of the surrounding area of one patient infected with C. echinulata could be identified as C. echinulata. High homology in the ITS region was found within the isolates of C. bertholletiae. Within the species C. echinulata and C. elegans a differentiation on subspecies level was achieved by an analysis of restriction fragment length polymorphism of the ITS amplicons after incubation with TaqI and HinfI. Similar results were obtained by PCR fingerprinting of the complete DNA with the single microsatellite DNA primers (GTG)5 and (GAC)5. For the first time C. echinulata could be identified as agent of zygomycosis in humans.
journal_name
Mycosesjournal_title
Mycosesauthors
Lemmer K,Losert H,Rickerts V,Just-Nübling G,Sander A,Kerkmann ML,Tintelnot Kdoi
10.1111/j.1439-0507.2002.tb04543.xsubject
Has Abstractpub_date
2002-01-01 00:00:00pages
31-6eissn
0933-7407issn
1439-0507journal_volume
45 Suppl 1pub_type
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