Abstract:
:An early diagnosis of an invasive fungal infection is essential for the initiation of a specific antifungal therapy and to avoid unnecessary discontinuation of a baseline therapy for haematological or oncological diseases. A real-time PCR assay for the detection and strain identification of Aspergillus species from culture strains was evaluated. DNA preparation was evaluated in contaminated culture media, urine and serum. A LightCycler PCR to differentiate various Aspergillus species was established. A real-time PCR assay for the detection of Aspergillus species was improved and was able to detect and differentiate medically important Aspergillus spp. The sensitivity of the test was <10 plasmid equivalents/assay. The real-time PCR assay is a useful tool for the rapid identification of Aspergillus species and might be useful as an early diagnostic tool to detect an invasive fungal infection. A real-time PCR protocol was improved by generating plasmid standards, additional generation of melting curves for species identification and the correlation between the melting temperature and the nucleotide exchanges within the used 18S rRNA gene region.
journal_name
Mycosesjournal_title
Mycosesauthors
Fricke S,Fricke C,Oelkrug C,Blatz R,Schönfelder U,Niederwieser D,Hilger N,Ruhnke M,Rodloff ACdoi
10.1111/j.1439-0507.2011.02161.xsubject
Has Abstractpub_date
2012-09-01 00:00:00pages
416-25issue
5eissn
0933-7407issn
1439-0507journal_volume
55pub_type
杂志文章相关文献
MYCOSES文献大全abstract::The incidence of tinea incognito (TI) appears to have increased over recent years, although no large series of cases has been reported in children. The aim of this study was to analyse the main epidemiological, clinical and microbiological characteristics of TI diagnosed in children in comparison with other tineas. We...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2009.01810.x
更新日期:2011-05-01 00:00:00
abstract::The Ustilaginomycetous basidiomycete yeast, Pseudozyma aphidis has recently been implicated in potentially fatal disorders ranging from subcutaneous mycoses to disseminated infections. Till date a solitary case of P. aphidis fungaemia in a paediatric patient has been reported. We present a case of fungaemia due to P. ...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/myc.12098
更新日期:2014-01-01 00:00:00
abstract::The phenomenon of persistent suppression of Candida albicans yeast cell growth after short drug exposures (postantifungal effect, PAFE) was determined by performing comparative studies using different concentrations of 5-fluorocytosine (5-FC), amphotericin B (AMPH) and miconazole (MCZ). An in vitro turbidometric metho...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.1991.tb00663.x
更新日期:1991-07-01 00:00:00
abstract::The authors report a case of subcutaneous mucormycosis in a diabetic patient with verrucous lesions in the fourth finger of the right hand. Initially diagnosed as a fixed cutaneous sporotrichosis case, success was obtained with the use of potassium iodide with healing of the lesion in a period of nearly 5 months. At p...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.1990.33.5.241
更新日期:1990-05-01 00:00:00
abstract::We report on a female patient who received a polychemotherapy because of an extensive pemphigus vulgaris. Under this therapy, the general condition of the patient worsened. Noncharacteristic symptoms, such as dyspnoea and tachycardia occurred. In spite of comprehensive, also invasive diagnostics, pulmonary foci demons...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.1994.tb00393.x
更新日期:1994-11-01 00:00:00
abstract::Although case series of talaromycosis have been reported in China, their detailed clinical and microbiological characteristics have never been systematically profiled. In this study, we report the clinical characteristics, molecular epidemiology, rapid identification and antifungal susceptibilities of talaromycosis in...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.12887
更新日期:2019-05-01 00:00:00
abstract::Invasive candidiasis is a common nosocomial infection among critically ill patients, constitutes an important cause of sepsis, and is associated with significant morbidity and mortality. The Infectious Diseases Society of America (IDSA) has created evidence-based guidelines for the management of invasive candidiasis. ...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0507.2008.01571.x
更新日期:2008-09-01 00:00:00
abstract::In an 84-year-old man cryptococcosis with a pronounced osteomyelitic course (involvement of a rib, two thoracic vertebrae, the sacrum and a femur) was diagnosed histologically post mortem, thus directing attention to cryptococcosis in advanced age. The presumptive chronic course of the Cryptococcus neoformans (Cr. n.)...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/myc.1990.33.4.157
更新日期:1990-04-01 00:00:00
abstract::In an open study 31 patients undergoing bone marrow transplantation for various haematological diseases received fluconazole as prophylaxis or treatment of fungal infections. In 26 of these patients an antecedent oral prophylaxis with polyene antimycotics had failed to prevent infections with Candida species. Five of ...
journal_title:Mycoses
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::A case of tinea capitis due to Microsporum gypseum in an adult is described. An otherwise healthy 69-year-old woman presented two large patches of slightly erythematous scaling alopecia localized on the vertex and on the left parietal region of the scalp. The only subjective sign was itching. A mycological culture was...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0507.1998.tb00331.x
更新日期:1998-05-01 00:00:00
abstract::Cryptococcus neoformans is a widely distributed saprophytic fungus that may cause opportunistic infections in normal and immunocompromised individuals particularly in patients with HIV infection. Disseminated infection in HIV-negative individuals is occasionally seen: a 57-year-old HIV-negative Turkish female initiall...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2005.01136.x
更新日期:2005-07-01 00:00:00
abstract::We have investigated the course of infection of Balb/c mice with Candida albicans. After intravenous infection of mice, the yeasts were evenly distributed in the liver sections. There was a 90% reduction in the yeasts found within 7 days after infection. This reduction was accompanied by an increase in the number of p...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.1997.tb00169.x
更新日期:1997-01-01 00:00:00
abstract::Histoplasmosis is an important systemic mycosis in the Americas, Asia, and Africa. Increasingly cases are recognised in nonendemic areas. Proper management requires recognition of the clinical syndromes caused by Histoplasma capsulatum infection, familiarity with the uses and limitations of the diagnostic tests, an un...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0507.2006.01253.x
更新日期:2006-07-01 00:00:00
abstract::The work reported here is the first nationwide, multicenter surveillance study conducted in Korea to obtain data on fluconazole susceptibility of Candida albicans (C. albicans) isolates. A total of 1137 isolates of C. albicans obtained from 17 university hospitals in South Korea during the 6-month period, July through...
journal_title:Mycoses
pub_type: 信件,多中心研究
doi:10.1111/j.1439-0507.2006.01329.x
更新日期:2007-03-01 00:00:00
abstract::This retrospective study reviews the occurrence and treatment of various forms of tinea capitis at the out-patient clinic for Dermatology and Pediatric Dermatology of the University Hospital Sophia/Dijkzigt over the period 1977-1988. Tinea capitis was diagnosed in 64 children. Trichophyton violaceum was the pathogen m...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.1990.33.1.46
更新日期:1990-01-01 00:00:00
abstract::Three cases of paracoccidioidomycosis observed in 1990-1991 in Quillabamba, Peru, and its surroundings are described. The climate and the living conditions of the inhabitants favour paracoccidioidomycosis. It can be assumed that a majority of cases could not be identified, because the symptoms are disguised as other t...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.1991.tb00866.x
更新日期:1991-11-01 00:00:00
abstract::This paper reports a case of chronic necrotizing pulmonary aspergillosis in a patient without underlying disease. Aspergilluria was the starting point in the search for the origin of the pulmonary disease, later confirmed by an open lung biopsy. ...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.1997.tb00216.x
更新日期:1997-10-01 00:00:00
abstract::A review is presented of the emerging problem of resistant and chronic vaginitis due to Candida glabrata. Although accurate numbers are lacking, several investigators report encountering increased numbers of women with vaginitis due to C. glabrata. Possible reasons for this apparent increase are discussed. Risk factor...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0507.1998.tb00594.x
更新日期:1998-01-01 00:00:00
abstract::Anti-Candida antibodies were determined in 2053 sera with an indirect hemagglutination test (HAT; Labor Diagnostika, Heiden, Germany) and with an enzyme immunoassay (ELISA) which detects IgG antibodies (Institut Virion, Würzburg, Germany). Antigen diagnostics was performed with the Cand-Tec-test (Ramco, Houston, USA)....
journal_title:Mycoses
pub_type: 临床试验,杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::Accurate identification of fungal pathogens using a sequence-based approach requires an extraction method that yields template DNA pure enough for polymerase chain reaction (PCR) or other types of amplification. Therefore, the objective of this study was to develop and standardise a rapid, inexpensive DNA extraction p...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/myc.12208
更新日期:2014-10-01 00:00:00
abstract::Malassezia spp. form part of the normal human cutaneous flora and are implicated in several mild, but recurrent cutaneous diseases, such as pityriasis versicolor, Malassezia folliculitis, seborrhoeic dermatitis, and, with lesser frequency, a range of other dermatological disorders. Malassezia spp. have also been assoc...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0507.2009.01814.x
更新日期:2010-05-01 00:00:00
abstract::Eighteen adult white male patients with cutaneous sporotrichosis were treated with itraconazole following different daily dose schemes. Cure was obtained in all cases after periods of 15-75 days (median 44 days) with total doses between 3.1 and 14.8 g (median 8.4 g). No serious side effects were observed and no relaps...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.1992.tb00836.x
更新日期:1992-05-01 00:00:00
abstract::Onychomycosis is common and can mimic several different nail disorders. Accurate diagnosis is essential to choose the optimum antifungal therapy. The aim of this study was to evaluate the use of confocal laser scanning microscopy (CLSM) and optical coherence tomography (OCT) as new non-invasive diagnostic tools in ony...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2012.02198.x
更新日期:2013-01-01 00:00:00
abstract::Rhinocerebral mucormycosis is a fulminant fungal infection of the nose and paranasal sinuses in immunocompromised patients. But mucormycosis following dental manipulation in immunocompetent patients leading to orbital involvement is rare. The objective of this study was to highlight the variable presentations of mucor...
journal_title:Mycoses
pub_type: 信件
doi:10.1111/j.1439-0507.2008.01600.x
更新日期:2009-07-01 00:00:00
abstract::The purpose of this study was to investigate the interaction between intravenous ampicillin-sulbactam treatment and (1,3)-beta-D-glucan (BDG) assay. Fifteen patients with a median age of 60 (16-81) without known risk factors for invasive fungal infections who received a daily dose of 3×2g ampicillin-sulbactam monother...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1111/j.1439-0507.2011.02131.x
更新日期:2012-07-01 00:00:00
abstract::It has always been difficult to treat onychomycosis due to decrease ability of topical agents to penetrate the nail and reach the affected nail bed. Oral antifungal have shown good response but due to longer duration course it has potential to cause systemic side effects, leading to patient non-adherence and adverse e...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/myc.12225
更新日期:2014-12-01 00:00:00
abstract::Airborne invasive fungal infections in various risk groups of people suffering from immunodeficiencies are an increasing problem for modern medicine. Because of the acute and rapid course of invasive infections, prevention is of principal significance. Such prevention mainly concerns the control of fungal spores in in...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0507.1992.tb00877.x
更新日期:1992-11-01 00:00:00
abstract::In 20 HIV-patients (17 male homosexuals, 1 male and 1 female i.v. drug abuser and 1 female patient with M. Willebrand-Jürgens) Candida esophagitis was diagnosed by esophagogastroduodenoscopy. Clinically they presented retrosternal pain or an exacerbation of oral candidosis under local antimycotics. The diagnosis of Ca...
journal_title:Mycoses
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::The mould collection of the Centraalbureau voor Schimmelcultures, Baarn, The Netherlands, was screened for isolates originating from warm-blooded animals. The range of species indicates that distribution of clinically relevant, pathogenic or opportunistic strains over the fungal kingdom is non-random. Some opportunist...
journal_title:Mycoses
pub_type: 杂志文章,评审
doi:10.1111/j.1439-0507.1992.tb00849.x
更新日期:1992-09-01 00:00:00
abstract::Different commercial enzyme-linked immunsorbent assays (ELISA) were evaluated in a preliminary study for diagnosis of systemic candidosis: Biomerica and Virotech GmbH, which allowed immunoglobulin G detection, and Platelia, which associated total antibody to antigen detection. They were tested with a home-made ELISA a...
journal_title:Mycoses
pub_type: 杂志文章
doi:10.1046/j.1439-0507.2002.00775.x
更新日期:2002-12-01 00:00:00